Buffer strips continue to feature in the management of agricultural runoff and water pollution in many countries. Existing research has explored their efficacy for reducing environmental problems in different geoclimatic settings but, the evidence on the efficacy of different vegetation treatments is less abundant than that for other buffer strip characteristics, including width, and is more contradictory in nature. With policy targets for various environmental outcomes including water or air quality and net zero pointing to the need for conversion of agricultural land, the need for robust experimental evidence on the relative benefits of different vegetation types in buffer strips is now renewed. Our experiment used a replicated plot scale facility to compare the efficacy of 12 m wide buffer strips for controlling runoff and suspended sediment loss during 15 sampled storms spanning 2017-2020. The buffer strips comprised three vegetation treatments: a deep rooting grass (Festulolium cv. Prior), a short rotation coppice willow and native broadleaved woodland trees. Over the duration of the monitoring period, reductions in total runoff, compared with the experimental control, were in the order: willow buffer strips (49%); deciduous woodland buffer strips (46%); grass buffer strips (33%). The corresponding reductions in suspended sediment loss, relative to the experimental control, were ordered: willow buffer strips (44%) > deciduous woodland buffer strips (30%) > grass buffer strips (29%). Given the 3-year duration of our new dataset, our results should be seen as providing evidence on the impacts during the establishment phase of the treatments.