Strict Interdependence Research Articles

"Metabolight": how light spectra shape plant growth, development and metabolism.

Innovations in light technologies (i.e. Light Emitting Diodes; LED) and cover films with specific optical features (e.g. photo-selective, light-extracting) have revolutionized crop production in both protected environments and open fields. The possibility to modulate the light spectra, thereby enriching/depleting cultivated plants with targeted wavebands has attracted increasing interest from both basic and applicative research. Indeed, the light environment not only influences plant biomass production but is also a pivotal factor in shaping plant size, development and metabolism. In the last decade, the strict interdependence between specific wavebands and the accumulation of targeted secondary metabolites has been exploited to improve the quality of horticultural products. Innovation in LED lighting has also marked the improvement of streetlamp illumination, thereby posing new questions about the possible influence of light pollution on urban tree metabolism. In this case, it is urgent and challenging to propose new, less-impacting solutions by modulating streetlamp spectra in order to preserve the ecosystem services provided by urban trees. The present review critically summarizes the main recent findings related to the morpho-anatomical, physiological, and biochemical changes induced by light spectra management via different techniques in crops as well as in non-cultivated species. This review explores the following topics: (1) plant growth in monochromatic environments, (2) the use of greenhouse light supplementation, (3) the application of covering films with different properties, and (4) the drawbacks of streetlamp illumination on urban trees. Additionally, it proposes new perspectives offered by in planta photomodulation.

A Multi-Domain Model for Variable Gap Iron-Cored Wireless Power Transmission System

Wireless power transfer (WPT) devices represent one of the most efficient and increasingly used technologies for the transfer of data and power in the near-field range. This work analyzes and describes a new type of device: a ferrite-cored, variable gap, high-frequency power and data transfer system. The classic theoretical models existing in the literature for near-field communication (NFC) and WPT devices have foreseen a lumped-parameters characterization based on the representation of an equivalent circuit model (ECM). The strict interdependence between the different physical domains has clearly increased the difficulty in predicting the behavior of the device, due to the unwanted continuous and chaotic variation of the parameters. The proposed paper aims to provide a general and reliable multi-physics model based on the co-simulation of a Spice®-based ECM analysis and the ESRF Radia®-based 3D finite volume methodology (3DFVM), placing particular emphasis on the intrinsic sensitivity with respect to variables that cannot be directly controlled, such as the variation of the air gap between the coupled coils interfaces. Furthermore, this work outlines a detailed and effective experimental methodology for the estimation of static and dynamic electro-magnetic parameters and the validation of the numerical models in both the time and frequency domain, through the analysis of a real coupled WPT device.

Lactobacillus Biofilms Influence Anti-Candida Activity.

Lactobacilli are the dominant members of the healthy human vaginal microbiota and represent the first defense line from pathogen infection, including vulvovaginal candidiasis. Biofilm is the predominant microbial growth form in nature, and the formation of biofilms inside the human body has important implications in health and disease. In particular, the formation of biofilm by members of the human resident microbiota is desirable, as it can improve microbial persistence and influence functionality. In the present study, we investigated the capability of 16 vaginal Lactobacillus strains (belonging to Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus vaginalis, and Lactobacillus plantarum species) to form biofilms, and we correlated their mode of growth to anti-Candida activity. L. plantarum strains were the best biofilm producers, and high variability was registered in the level of biofilm formation among L. crispatus and L. gasseri strains. Culture supernatants derived from Lactobacillus biofilm and planktonic growth were tested toward a panel of Candida clinical isolates (Candida albicans, Candida glabrata, Candida lusitaniae, Candida tropicalis, Candida krusei, and Candida parapsilosis) and their metabolome assessed by 1H-NMR. L. crispatus and L. plantarum strains exhibited the best fungistatic profile, and biofilms enhanced their anti-Candida activity; on the contrary, L. gasseri strains were more effective when grown in a planktonic mode. Biofilm/planktonic mode of growth also affects Lactobacillus metabolism, mainly influencing nitrogen and amino acid pathways, and anti-Candida activity is instead strictly related to carbohydrate metabolism. The present study underlined the strict interdependence between microbial mode of growth, metabolism, and functional properties. Biofilm formation by members of the healthy human microbiota represents a crucial issue in the field of microbial physiology and host–microbiota interactions, beyond supporting the development of new antimycotic strategies based on probiotics grown in adherence.

Recognising, Safeguarding, and Promoting Food Heritage: Challenges and Prospects for the Future of Sustainable Food Systems

The safeguarding and promotion of food heritage are often considered as a possible way for achieving social and cultural sustainability objectives. This literature review investigates some of the dynamics underlying the heritagisation of food and explores the risks of this process. It focuses mainly on anthropological, geographical, and sociological publications. Overall, it aims to shed light on the strengths and limitations of food heritagisation regarding the improvement of the socio-cultural sustainability of the food system. The analysis highlights cross-cutting risks, namely the omission of tangible and intangible elements of the local food system, and the exclusion of key stakeholders from the recognition and institutionalisation of food heritage. The review highlights the strict interdependence between intangible and tangible elements during food heritagisation, and assesses how local and global interactions can activate and shape this process. It sheds light on the need to pay more attention to the factors, actors, and relationships underpinning the emergence and recognition of food and food-related elements as part of the local heritage.

Modeling and assessing the effectiveness of investment projects in the agricultural sector

The problems of complementarity of the subordinate elements of the system of modeling and improving the mathematical accuracy of assessing the effectiveness of capital-intensive and long-term investment projects in the agricultural sector are presented. The solution to the problem of strict interdependence of model blocks is to create a rearrangeable and complemented cognitive optimization system for the target base center. The model consists of eight blocks, four of which are resource modeling blocks, and four are organizational and economic modeling blocks. The basis of the target base center is a cognitive system based on the work of the stochastic and deterministic method, which allows smoothing out complementarity and at the same time not going beyond the boundaries of established efficiency. In an unsteady economy, investment analysis must take into account the factors that change the value of money over time, uncertainty and risk. This requires a comprehensive and systematic analysis based on the logical and mathematical concept of the time value of money and discounting when forming the appraisal apparatus of investment projects. The principle of a logical and mathematical concept is proposed, which consists in the fact that only the accumulated amount can be discounted, not the capital gain. Arguments are presented in favor of the fact that it is mathematically incorrect to compensate for the effect of inflation, risk and the alternative cost of capital on cash flows of a real investment project by discounting cash inflows.

Modeling parameters of the production project

This article presents the problem of modeling and optimization of large-scale production project parameters. The creation of model structures and algorithms is associated with a strict interdependence of their elements. The authors propose the creation of the target base center of the model, which is characterized by multi-aspect study, dynamism and flexibility. The model consists of eight blocks, four of which – blocks of resource modeling, and four others – blocks of organizational and economic modeling. The basis of the target base center is a cognitive system based on the work of the stochastic and deterministic method, which allows one to smooth complementarity and not to go beyond the established efficiency.

Regression by Integration demonstrated on Ångström-Prescott-type relations

Abstract We present a novel approach for the determination of the relationship between two random variables, which we call Regression by Integration. The resulting curve is a least absolute error estimate. Compared to other regression methods, it has the advantage that, instead of a sample of simultaneously taken pairs of the two random variables, only a separate sample of each of the random variables is required. We demonstrate the practicability of the method on Angstrom-Prescott-type relations and compare the results with those obtained by least square error fits. We present supporting theoretical background information based on copulas. We show that Regression by Integration leads to the strict interdependence of the two random variables; Spearman's rho is equal to one.

The logical and pragmatic structure of arguments from analogy

The reasoning process of analogy is analyzed as a strict interdependence between a process of abstraction of a common feature and the transfer of an attribute of the Analogue to the Primary Subject. The first reasoning step is regarded as an abstraction of a generic characteristic that is relevant for the attribution of the predicate. The abstracted feature can be considered from a logic-semantic perspective as a functional genus, in the sense that it is contextually essential for the attribution of the predicate, i.e. that is pragmatically fundamental (i.e. relevant) for the predication, or rather the achievement of the communicative intention. While the transfer of the predicate from the analogue to the analogical genus and from the genus to the primary subject is guaranteed by the maxims, or rules of inference, governing the genus-species relation, the connection between the genus and the predicate can be complex, characterized by various types of reasoning patterns. The relevance relation can hide an implicit argument from classification, or an evaluation based on values, consequences or rules, or a causal relation, or an argument from practical reasoning.

Bridging the boundaries between scientists and clinicians-mechanistic hypotheses and patient stories in risk assessment of drugs.

The cultural divide between scientists and clinicians has been described as undermining the advance of medical science, by hindering the production of practice-relevant research and of research-informed clinical decisions. Here, I consider the field of post-marketing risk assessment of drugs as an example of strict interdependence between basic biomedical research, clinical research, and clinical evaluation and show how it would benefit from a closer collaboration between scientists and clinicians. The risk assessment of drugs after their marketing relies on spontaneous adverse effect reports to drug agencies and on peer-reviewed case reports. I emphasize the importance of qualitative analysis of such reports for the improvement of mechanistic understanding of harmful effects of drugs. I argue that mechanistic explanations of drug effects are at least as important as determination of their frequency, in order to establish causation. An ideal risk assessment, then, verifies not only the frequency of undesired effects but also why and how the harm happens. For this purpose, the frequency or novelty of the unintended outcome, although contextually indicative, should not determine the epistemic value of a report. Details about the context that generated an unexpected outcome, instead, can offer the chance of improving causal understanding about how the intervention works. This is illustrated through examples from medical research. Mechanistic understanding is a domain of joint collaboration among (1) clinicians, in charge of detailed, qualitative reporting of patient stories about side effects, (2) qualitative clinical researchers, in charge of analyzing clinical contexts or harmful effects and formulating explanatory hypotheses, and (3) basic biomedical researchers, in charge of verifying such hypotheses. In addition, direct information flow can on one side focus clinicians' attention on knowledge gaps about drugs/effects where more research is needed, while on the other side create a more contextualized concept of mechanism among scientists.

Body shape variation and colour change during growth in a protogynous fish

Protogynous sequential hermaphroditism is very common in marine fish. Despite a large number of studies on various aspects of sequential hermaphroditism in fish, the relationship between body shape and colour during growth in dichromatic species has not been assessed. Using geometric morphometrics, the present study explores the relationship between growth, body shape and colouration in Coris julis (L. 1758), a small protogynous labrid species with distinct colour phases. Results show that body shape change during growth is independent of change in colour phase, a result which can be explained by the biology of the species and by the social control of sex change. Also, during growth the body grows deeper and the head has a steeper profile. It is hypothesized that a deeper body and a steeper profile might have a function in agonistic interactions between terminal phase males and that the marked chromatic difference between colour phases allows the lack of strict interdependence of body shape and colour during growth.

The accessory subunit B of DNA polymerase is required for mitochondrial replisome function

The mitochondrial replication machinery in human cells includes the DNA polymerase γ holoenzyme and the TWINKLE helicase. Together, these two factors form a processive replication machinery, a replisome, which can use duplex DNA as template to synthesize long stretches of single-stranded DNA. We here address the importance of the smaller, accessory B subunit of DNA polymerase γ and demonstrate that this subunit is absolutely required for replisome function. The duplex DNA binding activity of the B subunit is needed for coordination of POLγ holoenzyme and TWINKLE helicase activities at the mtDNA replication fork. In the absence of proof for direct physical interactions between the components of the mitochondrial replisome, these functional interactions may explain the strict interdependence of TWINKLE and DNA polymerase γ for mitochondrial DNA synthesis. Furthermore, mutations in TWINKLE as well as in the catalytic A and accessory B subunits of the POLγ holoenzyme, may cause autosomal dominant progressive external ophthalmoplegia, a disorder associated with deletions in mitochondrial DNA. The crucial importance of the B subunit for replisome function may help to explain why mutations in these three proteins cause an identical syndrome.

L’épreuve de l’autre — Testing the other

Testing the other. It is nowadays a commonplace of academic discourse on social sciences, especially when it comes to such disciplines as anthropology and semiotics, to oppose the old (and old-fashioned) methods of the “structuralists” to post-modern and post-structural epistemological attitudes. Structuralism, it is said, was based on the idea that it is possible to apprehend the meaning of cultural productions from an exterior and therefore objective standpoint, just by making explicit their immanent principles of organization. Today, on the contrary, a totally distinct approach of cultural productions would stem from the consciousness of a strict interdependence, or even of an identity in nature between subject and object at all levels of the process of knowledge, at least in the area of the humanities. However, such a crude opposition proves insufficient when one observes the effective practices of current research. The example here analysed is the account given by the American anthropologist Paul Rabinow of his first mission abroad: Reflections on Fieldwork in Morocco. The analysis, based on the use of a semiotic modelling of interaction, consists in exploring the variety of positions respectively adopted by the anthropologist and his informants according to circumstances and contexts. Four regimes are in principle distinguishable: programmation, based on regularity and predictability of the actors’ behaviour, manipulation, based on some kind of contractualization of their relationships, adjustment, based upon reciprocal sensitivity and various strategies permitting to both partners of the interaction to test one another, and a regime of consent to the unexpected or the unforeseeable. The main result of the analysis resides in the possibility of showing that at each of these styles of pragmatic interaction corresponds a specific regime at the cognitive level as well. This leads to stressing the complexity, if not heterogeneity, of the strategies of knowledge involved at various stages of anthropological research, from the collection of data to the cooperative production of new forms of understanding. Taking the risk of generalization, one might also consider the interactional device, which is here tested through the reading of P. Rabinow’s report as a metatheoretical model describing the various epistemological stances at work and at stake in the practices of research in social sciences at large.

Dissociation of Corticothalamic and Thalamocortical Axon Targeting by an EphA7-Mediated Mechanism

Molecular mechanisms generating the topographic organization of corticothalamic (CT) circuits, which comprise more than three-quarters of the synaptic inputs onto sensory relay neurons, and their interdependence with thalamocortical (TC) axon development are unknown. Using in utero electroporation-mediated gene transfer, we show that EphA7-mediated signaling on neocortical axons controls the within-nucleus topography of CT projections in the thalamus. Notably, CT axons that mis-express EphA7 do not shift the relative positioning of their pathway within the subcortical telencephalon (ST), indicating that they do not depend upon EphA7/ephrin-A signaling in the ST for establishing this topography. Moreover, mis-expression of cortical EphA7 results in disrupted topography of CT projections, but unchanged inter- and intra-areal topography of TC projections. Our results support a model in which EphA/ephrin-A signaling controls independently the precision with which CT and TC projections develop, yet is essential for establishing their topographic reciprocity.

The Paradox of Self-Consciousness

In this book, Jose Luis Bermudez addesses two fundamental problems in the philosophy and psychology of self-consciousness: (1) Can we provide a noncircular account of fully fledged self-conscious thought and language in terms of more fundamental capacities? (2) Can we explain how fully fledged self-conscious thought and language can arise in the normal course of human development? Bermudez argues that a paradox (the paradox of self-consciousness) arises from the apparent strict interdependence between self-conscious thought and linguistic self-reference. The paradox renders circular all theories that define self-consciousness in terms of linguistic mastery of the first-person pronoun. It seems to follow from the paradox of self-consciousness that no such account or explanation can be given. Drawing on recent work in empirical psychology and philosophy, the author argues that any explanation of fully fledged self-consciousness that answers these two questions requires attention to primitive forms of self-consciousness that are prelinguistic and preconceptual. Such primitive forms of self-consciousness are to be found in somatic proprioception, the structure of exteroceptive perception, and prelinguistic forms of social interaction. The author uses these primitive forms of self-consciousness to dissolve the paradox of self-consciousness and to show how the two questions can be given an affirmative answer.

Use of a cell cycle mutant to delineate the critical period for the control of histone mRNA levels in the mammalian cell cycle.

Temporal analysis of DNA replication and histone mRNA accumulation in a hamster fibroblast cell cycle mutant (K12) showed that histone mRNA accumulates periodically during the cell cycle and reaches its highest level in the S phase. The direct correlation between the initiation of DNA synthesis and the accumulation of histone mRNA to high levels in S phase demonstrated the strict interdependence of these two events. Moreover, a critical period necessary for histone mRNA accumulation occurred late in G1 phase. If cells were incubated at the nonpermissive temperature during this critical period, the amount of histone mRNA remained at the basal level. Transcription rate measurements indicated that the triggering of histone mRNA synthesis occurred in late G1 and this mRNA was synthesized at its maximal rate 3 to 5 h before its peak of accumulation. However, if cells were prohibited from synthesizing DNA as a consequence of the temperature-sensitive block in G1, the synthesis of histone mRNA was not initiated.

Cladocere remains in recent sediments as indices of cultural eutrophication of Lake Como

Cladocera remains, organic matter, nitrogen, chlorophyll derivatives have been examined in the sediments to follow the trophic evolution of Lake Como. Trophic levels result to be increased in these last ten years in the four basins considered;Bosmina remains register recent and significant increases everywhere, whileSida, Acroperus, Daphnia andChydorus seem to disappear or on the strong decrease at present in the southern basins, more subject to the human pressure. Correlation coefficients are highly significant among organic matter, nitrogen, chlorophyll derivatives and Cladocera remains, giving stronger evidence for the strict interdependence among nutrients, producers and consumers.