Stretch Tension Research Articles

Exosomes from Tension Force-Applied Periodontal Ligament Cells Promote Mesenchymal Stem Cell Recruitment by Altering microRNA Profiles.

To investigate the role of exosomes from periodontal ligament cells (PDLCs) in bone marrow mesenchymal stem cell (BMSC) migration. Human PDLCs were applied cyclic tension stretching. Exosomes were extracted from cultured PDLCs by ultracentrifugation, then characterized for their size, morphology and protein markers by NTA, TEM and western blotting. The process that PKH26-labeled exosomes taken up by BMSCs was assessed by confocal microscope. BMSC migration was examined by Transwell assay. Exosomes derived from PDLCs were identified. Cyclic tension stretch application on PDLCs can enhance the migration ability of BMSCs through exosomes. The exosomal miRNA expression profiles of unstretched and stretched PDLCs were tested by miRNA microarray. Four miRNAs (miR-4633-5p, miR-30c-5p, miR-371a-3p and let-7b-3p) were upregulated and six (miR-4689, miR-8485, miR-4655-3p, miR-4672, miR-3180-5p and miR-4476) were downregulated in the exosomes after stretching. Sixteen hub proteins were found in the miRNA-mRNA network. Gene Ontology and KEGG pathway analyses demonstrated that the target genes of differentially expressed exosomal miRNAs closely related to the PI3K pathway and vesicle transmission. The exosomes derived from cyclic tension-stretched PDLCs can promote the migration of BMSCs. Alternation of microRNA profiles provides a basis for further research on the regulatory function of the exosomal miRNAs of PDLCs during orthodontic tooth movement.

P‐13.8: The Effect Analysis of FMM Tension Wrinkle According to the Shape Parameters of Pixel Slot Arrangement

P‐13.8: The Effect Analysis of FMM Tension Wrinkle According to the Shape Parameters of Pixel Slot Arrangement

Tunneled superior naso-labial island flap in post mucor infraorbital defect

<p class="abstract">Reconstruction of facial infraorbital defects, an untoward sequel of mucormycosis, is an issue confronting the rhinologist.<strong> </strong>Tunneled island flap was used in a patient with a wide defect involving the infraorbital region. Inspite of intricate pedicle dissection and likelihood of a trapdoor deformity these flaps facilitate repair in a single-staged procedure. The flap is so sited that its vascularity is not compromised neither by stretch tension or overlying tissue compression; while in the tunnel. The occasional, slight bulging is observed which is due to the pedicle; just over the undermined tunnel.<strong> </strong>This effect settles down with time and doesn’t need corrective intervention<strong>.</strong> Moreover<strong>,</strong> the donor site scars are merged in the margins of the aesthetic units thereby retaining central facial symmetry.</p>

3D in situ study of damage during a ‘shear to tension’ load path change in an aluminium alloy

A load path change (LPC) from shear to tension has been studied for a recrystallized 2198 T8 aluminium alloy sheet material by three-dimensional (3D) X-ray imaging combined with image correlation and interpreted by complementary 3D finite element (FE) simulations. A cross-shaped specimen was designed for the non-proportional loading and multiscale study. The effect of the LPC on the formability and related strain localisation, damage and failure was investigated and damage mechanisms could be clearly identified. The macroscopic tension stretch to fracture, measured by an optical extensometer during the shear to tension test, was reduced by about 20% compared to the proportional tension test. Damage, measured by in situ laminography imaging at μm-scale resolution, has interestingly already been found under shear, and was quantified as surface void fraction during the LPC. Strain was measured inside the material and at the mesoscale by 2D digital image correlation (DIC) on projected volume data, using the (natural) intermetallic particle contrast present in the 3D laminographic data. An accumulated equivalent strain definition, suited for the description of non-proportional loading, has been applied to the DIC data and FE simulations, indicating good agreement between both. On the microscopic scale, damage was seen to nucleate under shear load in the form of flat cracks, of similar width as the grain size, and in the form of cracks inside intermetallic particles. This damage subsequently grew and coalesced during tensile loading which in turn led to final fracture.

Acute Effects of Kinesiology Taping Stretch Tensions on Soleus and Gastrocnemius H-Reflex Modulations

This study examined the acute effects of stretch tensions of kinesiology taping (KT) on the soleus (SOL), medial (MG), and lateral (LG) gastrocnemius Hoffmann-reflex (H-reflex) modulation in physically active healthy adults. A cross-over within-subject design was used in this study. Twelve physically active collegiate students voluntarily participated in the study (age = 21.3 ± 1.2 years; height = 175.6 ± 7.1 cm; body weight = 69.9 ± 7.1 kg). A standard Y-shape of KT technique was applied to the calf muscles. The KT was controlled in three tension intensities in a randomised order: paper-off, 50%, and 100% of maximal stretch tension of the tape. The peak-to-peak amplitude of maximal M-wave (Mmax) and H-reflex (Hmax) responses in the SOL, MG, and LG muscles were assessed before taping (pre-taping), taping, and after taping (post-taping) phases in the lying prone position. The results demonstrated significantly larger LG Hmax responses in the pre-taping condition than those in the post-taping condition during paper-off KT (p = 0.002). Moreover, the ΔHmax/Mmax of pre- and post-taping in the SOL muscle was significantly larger during 50%KT tension than that of paper-off (p = 0.046). In conclusion, the stretch tension of KT contributes minor influence on the spinal motoneuron excitability in the triceps surae during rest.

Fate of a bulge in an inflated hyperelastic tube: theory and experiment

Mechanical instability in a pre-tensioned finite hyperelastic tube subjected to a slowly increasing internal pressure produces a spatially localized bulge at a critical pressure. This instability is studied in controlled experiments on inflated latex rubber tubes, from the perspective of buckling observed in aneurysms and their rupture risk. The fate of the bulge under continued inflation is governed by the end-conditions and the initial tension in the tube. In a tube with one end fixed and a weight attached to the other freely moving end, the bulge propagates axially at low initial tension, growing in length, and the tube relaxes by extension without buckling. Rupture occurs when the tension is high. By contrast, the bulge formed in an initially stretched tube held fixed at both its ends can buckle or rupture, depending on the amount of initial tension. Experiments are reported for different initial tensions and boundary conditions (BCs). Failure maps in the stretch parameter space and in stretch–tension space are constructed by extending existing theories for bulge formation and buckling analyses to the experimentally relevant BCs. Failure maps deduced from the theory are compared against experiments, and the underlying assumptions are critically assessed. Experiments reveal that buckling provides an alternative route to relieve the stress built up during inflation. Hence, buckling, when it occurs, can be a protective fail-safe mechanism against the rupture of a bulge in an inflated elastic tube.

Moderate kinesio taping stretch tension produced good inter-day reliability of dorsiflexion joint position sense measurement

Moderate kinesio taping stretch tension produced good inter-day reliability of dorsiflexion joint position sense measurement

Mathematical modeling in the problems of the geodesic control of the stressed-formated state of the gas pipelines at above-ground parts

Geodetic methods of monitoring the stress-strain state of gas pipelines in the areas of overhead pipeline crossing involve obtaining discrete data on the deformation of its axis in the form of a set of three coordinates (x, y, z). For their elaboration a mathematical model of the experimental-theoretical method of geodetic control of gas pipelines’ SSS is proposed. The simulation process was performed on the basis of geodetic discrete data, based on which, using the Lagrange interpolation polynomial, the equation of the line of bending of the axis of the gas pipeline was obtained, which allowed to pass further to the bending moments along the entire span. The accuracy of the proposed method has been estimated, which showed that the obtained bending moment diagrams reflect the SSS of gas pipelines with an accuracy of 1.5% in the central part of its span, and with the approach to the peripheral sections the error increases to 6%. It is shown that the obtained expression of the maximum bending moment allows us to form the required number of additional equations for the disclosure of a statically indeterminate system of "gas pipeline-support", taking into account the possibility of fastening on stretch marks. The mathematical model is presented aimed at decomposition of overburden gas pipeline structures, which allows to model the SSS at each of the spans separately within the integral structure.. It is established that additional stretch marks reduce the maximum bending stress by 22%, and in case of loss of one of the stretches of the bearing capacity of the gas pipeline SSS increases by 11.1-14.8% depending on the previous stretch tension. The proposed mathematical model for the processing of discrete data forms the basis of the experimental-theoretical method of geodetic control of the gas pipelines’ SSS, taking into account their structural and technological factors.

Intramural Injection of Botulinum Toxin A in Surgical Treatment of a Long Gap Esophageal Atresia-Rat Model.

Anastomosis with minimal tension is desirable in long-gap esophageal atresia. Prior studies in piglets showed that intraesophageal injection of botulinum toxin type A (BTX-A) results in significant esophageal elongation. Our aim was to determine the BTX-A dose, number of injections, and time necessary to elicit maximal response. Adult male Wistar rats (n = 48) were randomly assorted into five groups. Four treatment groups received 2 or 4 U/kg of BTX-A, delivered using two or four injections, and a control group received 0.9% NaCl. Esophagus was removed 6 or 24-hours postinjection and tested ex vivo using a stretch tension device. Subsequently, an optimal dose and time following injection was used to study the effects of BTX-A on anastomotic healing in vivo. Rats (n = 12) received an intraesophageal injection of BTX-A or 0.9% NaCl, followed by resection of 0.5 cm of esophagus and end-to-end anastomosis. Rats were observed for 9 days, and esophagus was removed for gross and histological evaluation. The largest effect on elongation was recorded in the BTX-A (2 U/kg) 24 hour, four injection group. In the anastomosis study, stricture formation was observed in all animals in the control group. Absence of esophageal stricture was found in three out of four animals in the treatment group macroscopically and histologically. We found that BTX-A exerts a positive effect on stretch characteristics of esophageal tissue in rats at 2 U/kg via four-injection delivery and 24-hour waiting period. This study suggests that BTX-A might improve anastomotic healing.

Poly-ε-caprolactone scaffold for the reinforcement of stapled small intestinal anastomoses: a randomized experimental study.

Anastomotic leakage is a severe complication in gastrointestinal surgery. Different methods have been evaluated for anastomotic reinforcement to prevent anastomotic leakage. The aim of this study was to investigate the effect of a poly-ε-caprolactone (PCL) scaffold incorporated in the staple-line, on the anastomotic strength and histological wound healing, of small intestinal anastomoses in piglets. This randomized experimental trial included 17 piglets. In each piglet, three end-to-end anastomoses were performed in the small intestine with a circular stapler, i.e. one control and two interventional anastomoses. On postoperative day 5, the anastomoses were resected and subjected to tension stretch test and histological examination. No anastomotic leakage occurred. In the interventional anastomoses, the mean value for maximal tensile strength was 15.7 N, which was significantly higher than control anastomoses 12.7 N (p = 0.01). No statistically significant differences were found between the two groups in the histopathological parameters. To conclude, this study has shown that the incorporation of a PCL scaffold in the staple-line was feasible and significantly increased the maximal tensile strength of small intestine anastomoses in piglets on postoperative day 5. The difference in histological parameters was not significantly distinct.

Fgfr2 is integral for bladder mesenchyme patterning and function.

While urothelial signals, including sonic hedgehog (Shh), drive bladder mesenchyme differentiation, it is unclear which pathways within the mesenchyme are critical for its development. Studies have shown that fibroblast growth factor receptor 2 (Fgfr2) is necessary for kidney and ureter mesenchymal development. Our objective was to determine the role of Fgfr2 in bladder mesenchyme. We used Tbx18cre mice to delete Fgfr2 in bladder mesenchyme (Fgfr2BM-/-). We performed three-dimensional reconstructions, quantitative real-time PCR, in situ hybridization, immunolabeling, ELISAs, immunoblotting, void stain on paper, ex vivo bladder sheet assays, and in vivo decerebrated cystometry. Compared with controls, embryonic (E) day 16.5 (E16.5) Fgfr2BM-/- bladders have thin muscle layers with reduced α-smooth muscle actin levels and thickened lamina propria with increased collagen expression that intrudes into muscle. From postnatal (P) day 1 (P1) to P30, Fgfr2BM-/- bladders demonstrate progressive muscle loss and increased collagen expression. Postnatal Fgfr2BM-/- bladder sheets exhibit decreased contractility and increased passive stretch tension compared with controls. In vivo cystometry revealed high baseline and threshold pressures and shortened intercontractile intervals in Fgfr2BM-/- bladders compared with controls. Mechanistically, while Shh expression appears normal, mRNA and protein readouts of hedgehog activity are increased in E16.5 Fgfr2BM-/- bladders compared with controls. Moreover, E16.5Fgfr2BM-/- bladders exhibit higher levels of Cdo and Boc, hedgehog coreceptors that enhance sensitivity to Shh, than controls. Fgfr2 is critical for bladder mesenchyme patterning by virtue of its role in modulation of hedgehog signaling.

A discrete network model to represent the deformation behavior of human amnion

A discrete network model (DNM) to represent the mechanical behavior of the human amnion is proposed. The amnion is modeled as randomly distributed points interconnected with connector elements representing collagen crosslinks and fiber segments, respectively. This DNM is computationally efficient and allows simulations with large domains. A representative set of parameters has been selected to reproduce the uniaxial tension–stretch and kinematic responses of the amnion. Good agreement is found between the predicted and measured equibiaxial tension–stretch curves. Although the model represents the amnion phenomenologically, model parameters are physically motivated and their effect on the tension–stretch and in-plane kinematic responses is discussed. The model is used to investigate the local response in the near field of a circular hole, revealing that the kinematic response at the circular free boundaries leads to compaction and strong alignment of the network at the border of the defect.

Fgfr2 is integral for bladder mesenchyme patterning and function.

While urothelial signals, including sonic hedgehog (Shh), drive bladder mesenchyme differentiation, it is unclear which pathways within the mesenchyme are critical for its development. Studies have shown that fibroblast growth factor receptor (Fgfr)2 is necessary for kidney and ureter mesenchymal development. The objective of the present study was to determine the role of Fgfr2 in the bladder mesenchyme. We used Tbx18cre mice to delete Fgfr2 in the bladder mesenchyme (Fgfr2(BM-/-)). We performed three-dimensional reconstructions, quantitative real-time PCR, in situ hybridization, immunolabeling, ELISAs, immunoblot analysis, void stain on paper, ex vivo bladder sheet assays, and in vivo decerebrated cystometry. Compared with control bladders, embryonic day 16.5 (E16.5) Fgfr2(BM-/-) bladders had thin muscle layers with less α-smooth muscle actin and thickened lamina propria with increased collagen type Ia and IIIa that intruded into the muscle. The reciprocal changes in mutant layer thicknesses appeared partly due to a cell fate switch. From postnatal days 1 to 30, Fgfr2(BM-/-) bladders demonstrated progressive muscle loss and increased collagen expression. Postnatal Fgfr2(BM-/-) bladder sheets exhibited decreased agonist-mediated contractility and increased passive stretch tension versus control bladder sheets. Cystometry revealed high baseline and threshold pressures and shortened intercontractile intervals in Fgfr2(BM-/-) versus control bladders. Mechanistically, whereas Shh expression appeared normal, mRNA and protein readouts of hedgehog activity were increased in E16.5 Fgfr2(BM-/-) versus control bladders. Moreover, E16.5 Fgfr2(BM-/-) bladders exhibited higher levels of Cdo and Boc, hedgehog coreceptors that enhance sensitivity to Shh, compared with control bladders. In conclusion, loss of Fgfr2 in the bladder mesenchyme leads to abnormal bladder morphology and decreased compliance and contractility.

Inferring coarse-grain histone-DNA interaction potentials from high-resolution structures of the nucleosome

The histone-DNA interaction in the nucleosome is a fundamental mechanism of genomic compaction and regulation, which remains largely unknown despite increasing structural knowledge of the complex. In this paper, we propose a framework for the extraction of a nanoscale histone-DNA force-field from a collection of high-resolution structures, which may be adapted to a larger class of protein-DNA complexes. We applied the procedure to a large crystallographic database extended by snapshots from molecular dynamics simulations. The comparison of the structural models first shows that, at histone-DNA contact sites, the DNA base-pairs are shifted outwards locally, consistent with locally repulsive forces exerted by the histones. The second step shows that the various force profiles of the structures under analysis derive locally from a unique, sequence-independent, quadratic repulsive force-field, while the sequence preferences are entirely due to internal DNA mechanics. We have thus obtained the first knowledge-derived nanoscale interaction potential for histone-DNA in the nucleosome. The conformations obtained by relaxation of nucleosomal DNA with high-affinity sequences in this potential accurately reproduce the experimental values of binding preferences. Finally we address the more generic binding mechanisms relevant to the 80% genomic sequences incorporated in nucleosomes, by computing the conformation of nucleosomal DNA with sequence-averaged properties. This conformation differs from those found in crystals, and the analysis suggests that repulsive histone forces are related to local stretch tension in nucleosomal DNA, mostly between adjacent contact points. This tension could play a role in the stability of the complex.

Material Parameters Determination and Simulation of Rubber Bushing

It is important for vehicle controllability and comfort to reliably determine the material parameters and accurately simulate the mechanical properties of rubber components. To meet the requirements of the analysis of rubber components in car industry,material test is designed and uniaxial stretch, uniaxial compression, and planer tension are performed to the rubber material of the control arm. The influences of sample difference, precondition, loading speed, and clamping method on the test results are analyzed.A reliable test method is proposed. The experimental data are processed and used to fit material parameters. The static rigidity test of the rubber bushing is performed and simulated with the determined material parameters. The simulation agrees well with the test. The results show that the material test method and the experimental data fitting method can reliably determine the rubber material parameters for finite element simulation.

The Determination of Optimal Initial Tension in Rat Coronary Artery Using Wire Myography

The aim of the present study was to determine the optimal initial tension, i.e. initial stretch for rat coronary artery when using the multi-wire myograph system. We used the normalization procedure to mimic physiological conditions and to stretch the coronary arterial segments to normalized internal circumference (IC(1)). It is determined the internal circumference when the vessel relaxed under a transmural pressure of 100 mm Hg (IC(100)), and the IC(1) is calculated by multiplying the IC(100) by a factor k. The impact of different factor k on the initial stretch and agonist-induced tension of coronary arteries were investigated. The results showed that the maximal agonist-induced tension was achieved at the factor k value of 0.90 and the initial stretch tension was given 1.16+/-0.04 mN/mm. The most appropriate factor k value was 0.90-0.95 and the most appropriate initial tension was 1.16-1.52 mN/mm. The equilibration time of the coronary artery segments should be at least 1.0 h. In the same optimal initial tension, the agonist-induced tension increased as equilibration time lengthened.

Passive stretch reduces calpain activity through nitric oxide pathway in unloaded soleus muscles

Unloading in spaceflight or long-term bed rest induces to pronounced atrophy of anti-gravity skeletal muscles. Passive stretch partially resists unloading-induced atrophy of skeletal muscle, but the mechanism remains elusive. The aims of this study were to investigate the hypotheses that stretch tension might increase protein level of neuronal nitric oxide synthase (nNOS) in unloaded skeletal muscle, and then nNOS-derived NO alleviated atrophy of skeletal muscle by inhibiting calpain activity. The tail-suspended rats were used to unload rat hindlimbs for 2 weeks, at the same time, left soleus muscle was stretched by applying a plaster cast to fix the ankle at 35° dorsiflexion. Stretch partially resisted atrophy and inhibited the decreased protein level and activity of nNOS in unloaded soleus muscles. Unloading increased frequency of calcium sparks and elevated intracellular resting and caffeine-induced Ca(2+) concentration ([Ca(2+)]i) in unloaded soleus muscle fibers. Stretch reduced frequency of calcium sparks and restored intracellular resting and caffeine-induced Ca(2+) concentration to control levels in unloaded soleus muscle fibers. The increased protein level and activity of calpain as well as the higher degradation of desmin induced by unloading were inhibited by stretch in soleus muscles. In conclusion, these results suggest that stretch can preserve the stability of sarcoplasmic reticulum Ca(2+) release channels which prevents the elevated [Ca(2+)]i by means of keeping nNOS activity, and then the enhanced protein level and activity of calpain return to control levels in unloaded soleus muscles. Therefore, stretch can resist in part atrophy of unloaded soleus muscles.

The role of neural tension in hamstring flexibility

Resistance to stretch, electromyographic (EMG) response to stretch, stretch discomfort and maximum range of motion (ROM) were measured during passive hamstring stretches performed in the slump test position (neural tension stretch) and in the upright position (neutral stretch) in eight healthy subjects. Stretches were performed on an isokinetic dynamometer at 5°/s with the test thigh flexed 40° above the horizontal, and the seat back at 90° to the horizontal. Surface EMG signals were recorded from the medial and lateral hamstrings during stretches. Knees were passively extended to maximum stretch tolerance with test order (neural tension vs neutral) alternated between legs. For neural tension stretches, the cervical and thoracic spine were manually flexed. Maximum ROM was 8° less for the neural tension stretch vs the neutral stretch (P<0.01). Resistance to stretch was 14-15% higher for the neural tension stretch vs the neutral stretch (P<0.001) at common joint angles in the final third of ROM. Stretch discomfort and EMG response were unaffected by neural tension. In conclusion, an increased passive resistance to stretch with the addition of neural tension during passive hamstring stretch despite no change in the EMG response indicates that passive extensibility of neural tissues can limit hamstring flexibility.

The Contribution of Calcium and Stretch-Activated Tension to Power Generation by Drosophila Indirect Flight Muscle

Recent studies suggest small insects might regulate muscle power output by operating their stretch activated indirect flight muscles (IFM) at less than saturating calcium levels during flight instead of varying the number of muscle fibers recruited. Calcium levels have also been found to correlate with wing beat frequency, suggesting calcium concentration might influence aerodynamic power. To test the effects of calcium concentration on muscle power generation, we used the work loop technique to measure Drosophila indirect flight muscle (IFM) oscillatory power generation under optimal length change parameters. Maximum power was 2.2±0.4 nW/mm3 at pCa = 4.7±0.09. The threshold for muscle power generation occurs at ∼ pCa 6.1, with pCa50 = 5.8±0.05 and the Hill coefficient = 7.2±3.7. Over the steepest portion of the curve, and above the estimated minimum power required for flight, about pCa 6.0 to 5.5, muscle power increases 2.5-fold. We compared the contributions to muscle power from calcium activated isometric tension to the contribution from the tension generated by stretch-activation during flight by imposing a 2.5% ML, 1.5 ms stretch. The pCa50 for calcium activated tension was 6.1±0.05 and Hill coefficient was 4.6±1.3. The pCa50 for stretch activated tension was 5.8±0.07 and Hill coefficient was 3.8±1.3. Following a stretch at pCa 4.5, total isometric tension increased from 2.5±0.3 mN/mm2 to 16.4±1.2 mN/mm2 of which 11.4±1.1 mN/mm2 was contributed by passive elastic elements. As calcium is increased from pCa 6.0 to 5.5, stretch-activated tension contributes 1.0±0.3 mN/mm2 of additional tension while calcium tension contributes 0.4±0.2 mN/mm2. We conclude that if calcium levels vary between pCa 6.0 to 5.5 during flight, then the contribution of stretch tension to additional power is about twice that of calcium tension.

サポーターの装着圧と伸縮性が上腕の筋疲労に及ぼす生理的効果

The purpose of this study is to examine tightening pressure of the supporter and effect of the elastic property on muscle fatigue through electromyogram measurement. Wearing a magic tape style taping supporter, several healthy students carried out the flexion experiment of the elbow. At this time, the electromyogram of biceps brachii was measured, and MFCV(Muscle Fiber Conduction Velocity) by which, the muscle fatigue index was calculated. As a result, it became clear that the tightening pressure of the supporter which relieves fatigue was 21-28gf/cm2. The elastic property of the supporter which relieved muscle fatigue was almost 0.8kgf/cm as a tension stretch. Local clothes pressure seems to affect that the supporter with the moderate elastic property is fixed in the appropriate tightening pressure in muscle contraction and muscle blood flow. This was assumed to be the reason why muscle fatigue is reduced.