In areas of low disturbance, such as the Aysén Region of Chile, the presence of roads can inadvertently facilitate the spread of invasive species. To address this issue, it is imperative to maintain up-to-date biological inventories, as they serve as a primary source of information for the conservation of species and ecosystems. However, the maintenance of systematic inventories of vascular plants in Chile is virtually non-existent, especially outside protected wilderness areas. The data we have come from an inventory of vascular plant species along a stretch of coastal road in Puerto Cisnes (Aysén Region), characterised by a cut slope in the rock. The site is located between mountain ranges, in a region known for its protected wilderness areas and low levels of anthropogenic alteration. The study adopted an observational sampling design, using the road as a transect. For each species identified, the growth substrate, habit and dispersal mode were recorded. A total of 70 species (36 herbs, 23 shrubs and 11 trees) belonging to 42 families were found. The most represented families were Hymenophyllaceae (nine species) and Myrtaceae (four species). We recorded nine introduced species belonging to seven botanical families (Cirsiumvulgare (Savi) Ten., Crocosmiacrocosmiiflora (Lemoine ex Burb. & Dean) N.E.Br., Cytisusscoparius (L.) Link, Digitalispurpurea L., Lotuspedunculatus Cav., Plantagolanceolata L., Polygonumcampanulatum Hook. f., Prunellavulgaris L., Rubusconstrictus Lefèvre & P.J.Müll). Of these nine species, seven are invasive, while the remaining two species have not been assessed for invasive potential (i.e. Crocosmiacrocosmiiflora and Polygonumcampanulatum). In particular, Crocosmiacrocosmiiflora and Rubusconstrictus are new regional records. The majority of species were found growing on the ground (44 species), while a significant proportion were found exclusively on rocky slopes (17 species). According to their seed dispersal mechanism, the most common syndromes were anemochory (32 species) and ornithochory (20 species). Other mechanisms such as mammalochory, ballochory or myrmecochory were less common (less than four species). This study provides valuable data on the vascular flora of Puerto Cisnes, Chile, a modest human settlement in a minimally altered landscape. The region, dominated by native forests and a burgeoning salmon farming industry, has few inventories, so the database presented here adds significantly to local botanical knowledge. The main novelty of this research is that it is the first inventory carried out on a road in a slightly altered area surrounded by protected wilderness areas (such as Magdalena Island National Park and Queulat National Park). The study systematically categorises species according to substrate, habitat and dispersal mode, dimensions that are rarely combined in a single database.The inventory identifies 70 species (36 herbs, 23 shrubs and 11 trees) in 42 families. The most represented families were Hymenophyllaceae (with nine species) and Myrtaceae (with four species). Additionally, we recorded, two introduced species (Crocosmiacrocosmiiflora and Rubusconstrictus) at least 100 km south of their known distribution.
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