Stress Measurements Research Articles

Idiopathic male infertility – what are we missing?

ABSTRACT Couple’s infertility is a rising issue worldwide affecting approximately 15% of couples. In 50% of the couples, a male factor infertility can be identified. Moreover, 30% of the men exhibit reduced sperm quality without any identifiable reason, thereby delineating the condition of idiopathic male infertility (IMI). Despite numerous improvements in the diagnosis and treatment of male infertility over the last decades, idiopathic forms are still the most challenging clinical dilemmas. The aim of this article is to describe the comprehensive diagnostic work-up that each idiopathic infertile man should follow. Moreover, potential new pathophysiological mechanisms and suggested treatment options are discussed. A detailed medical history and an extensive physical examination are mandatory to investigate potential treatable causes of MFI. Similarly, standard semen analysis has been proven to be limited in capturing the fecundability of the spermatozoa itself, therefore more advanced examinations, such as sperm DNA fragmentation (SDF) and oxidative stress measurement, are becoming important in clinical practice for IMI. In terms of diagnostic tools, imaging and genetic investigations are useful to classify idiopathic infertile men, however, epigenetic changes have demonstrated to have a role in sperm production and a prognostic value in fertility outcomes. Antioxidant treatment for IMI has been found to be a valid option to counteract ROS action, while gonadotropins are used to improve sperm quality and SDF. Artificial intelligence is promising to better manage idiopathic infertile men in terms of diagnosis and treatment options.

Cardiovascular physiology of decapod crustaceans: from scientific inquiry to practical applications.

Until recently, the decapod crustacean heart was regarded as a simple, single ventricle, contraction of which forces haemolymph out into seven arteries. Differential tissue perfusion is achieved by contraction and relaxation of valves at the base of each artery. In this Review, we discuss recent work that has shown that the heart is bifurcated by muscular sheets that may effectively divide the single ventricle into 'chambers'. Preliminary research shows that these chambers may contract differentially; whether this enables selective tissue perfusion remains to be seen. Crustaceans are unusual in that they can stop their heart for extended periods. These periods of cardiac arrest can become remarkably rhythmic, accounting for a significant portion of the cardiac repertoire. As we discuss in this Review, in crustaceans, changes in heart rate have been used extensively as a measurement of stress and metabolism. We suggest that the periods of cardiac pausing should also be quantified in this context. In the past three decades, an exponential increase in crustacean aquaculture has occurred and heart rate (and changes thereof) is being used to understand the stress responses of farmed crustaceans, as well as providing an indicator of disease progression. Furthermore, as summarized in this Review, heart rate is now being used as an effective indicator of humane methods to anaesthetize, stun or euthanize crustaceans destined for the table or for use in scientific research. We believe that incorporation of new biomedical technology and new animal welfare policies will guide future research directions in this field.

The impact of cognitive fusion on stress among nurses: Exploring gender as a moderator controlling for depressive symptoms.

To investigate the influence of cognitive fusion on stress among nurses, with a focus on exploring gender as a moderator among Jordanian nurses controlling for depressive symptoms. Stress is a significant concern among healthcare workers, particularly nurses, given the escalating work demands and organizational constraints. Data were collected from 206 nurses using a cross-sectional design. Participants completed surveys that included measures for cognitive fusion, stress, and depressive symptoms. Statistical analysis included bivariate correlations, independent sample t-tests, and moderation analysis using Hayes PROCESS. The results were reported using The Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines. The findings revealed a moderate correlation among stress, cognitive fusion, and depressive symptoms. The regression model explained 42% of the variance in stress, with significant contributions from cognitive fusion, gender, depressive symptoms, and the interaction between cognitive fusion and gender. Gender moderated the relationship between cognitive fusion and stress, indicating differential effects for males and females. This study underscores the importance of cognitive fusion in understanding and managing stress among nurses. The moderating effect of gender highlights the need for tailored interventions. Reducing stress among nurses may benefit their mental health and potentially enhance patient care and treatment outcomes. IMPLICATIONS FORNURSING PRACTICE AND POLICY: The study highlights crucial implications for nursing policy. Addressing cognitive fusion is vital to reducing nurse stress, directly impacting patient care quality and healthcare outcomes. Policymakers should prioritize gender-aware strategies within nursing policies, fostering a less stressful and more supportive work environment. By doing so, healthcare institutions can enhance the well-being of nurses and ultimately improve patient care, aligning with broader healthcare policy objectives of patient safety and quality improvement.

Temperature calibration of sample rods for neutron diffraction apparatus in the range of 6–473 K

Abstract Internal stresses play a crucial part in determining a material’s properties, which emphasizes the significance of accurate stress measurement and analysis. Neutron diffraction technology is a very promising approach to studying the complex microstructural properties of many materials, especially with the growing demand for cryogenic studies. This new device has a robust load capacity of 50 kN and works smoothly in a wide temperature range of 6 K–473 K. Furthermore, the diffraction angle is 47 degrees broader. Regardless of the samples, we observed that a thermal balance point existed close to 180 K. Importantly, thermal resistance was eliminated by the use of an analytical method, which has an extraordinarily small error of 2%. Using this exact calibration methodology ensures that the sample temperature is accurate during the experiments. This work presents a crucial apparatus for investigating the complexity of internal stresses in materials and offers an effective method for estimating and managing sample temperatures while doing research.

Examining the association of familial and social stress, trauma, and support on mood, anxiety, and behavioral symptoms and diagnoses in youth at risk for bipolar disorder and controls

IntroductionYouth with a family history of bipolar disorder (At-Risk) have a higher risk of developing psychiatric disorders and experiencing environmental stressors than youth without such family history (Control). We studied the differential associations of familial and environmental factors on developing psychiatric diagnoses and symptoms, in At-Risk and Control youth. MethodsAt-Risk and Control youth (N = 466, ages 9–22) were systematically assessed for severity of symptoms, psychiatric diagnoses, and self-reported measures of stress and social support. We tested the association of family history and measures of stress or support with symptom severity and diagnoses. ResultsAt-Risk youth had higher symptom severity scores and were more frequently diagnosed with psychiatric disorders (all p values < 0.001). When predicting mood symptom severity, family history had an interaction effect with stressful life events (p < 0.001) and number of distinct traumatic events (p = 0.001). In multivariate models, At-Risk status predicted anxiety disorders (OR = 2.7, CI 1.3–5.4, p = 0.005) and anxiety severity (Coefficient = 0.4, CI 0.2–0.7, p < 0.001) but not mood or behavioral disorder diagnoses or severity. LimitationsMeasures of stress and social support were based on self-report. Not all participants had passed through the period of risk for developing the outcomes under study and the follow up period was variable. We could not fully study the differential impact of physical or sexual abuse due to low frequency of occurrence in controls. ConclusionAt-Risk youth exhibit more severe mood symptoms compared to Controls when exposed to similar levels of stress or trauma. At-Risk youth are also more prone to develop anxiety which may be a precursor for bipolar disorder.

Critical consciousness among undocumented immigrant college students: Responding to cultural stress and psychological distress.

Cultural stress is experienced by undocumented students when they encounter discrimination in higher education and society. In response, undocumented students engage in critical consciousness, while simultaneously experiencing psychological distress. A conceptual model is introduced to describe the links between cultural stress, critical consciousness development, and psychological distress. One hundred seventy-one undocumented college students were recruited to participate in this study and completed measures of cultural stress (discrimination). They also completed measures of psychological distress, critical consciousness, and social justice self-efficacy and outcome expectations. The proposed model was tested for global fit and path analysis in structural equation modeling. Indirect effects were probed to assess the mediating role of critical consciousness and psychological distress. The model fit the data excellently. Greater discrimination was associated with higher critical consciousness, which in turn was associated with elevated social justice self-efficacy and outcome expectations. Additionally, higher discrimination was also linked to greater psychological distress. The link between cultural stress and critical consciousness was not explained by psychological distress. This research expands theory by articulating the adaptive ways undocumented students respond to the conditions that cause cultural stress and psychological distress. This aligns with a growing literature documenting the psychology of undocumented immigrants' activism and advocacy. Findings may inform interventions by higher education institutional agents and research to offset cultural stress and psychological distress. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

A Preliminary Analysis of Stress Burden and Cognitive Function and Clinically-Adjudicated Cognitive Outcomes in Black American Adults.

The combination of exposure to multiple stressors and psychological distress may contribute to the disproportionate burden of dementia risk among Black Americans. This study estimates the effect of an index of stress and psychological distress (i.e., "stress burden") on cognitive function and clinically-adjudicated cognitive outcomes among older Black American adults, and examines sleep as a mediator. The sample included 204 Black adults (79% female; mean age=64 years) from Pittsburgh, PA. Stress burden comprised three self-reported stress and distress measures assessed in 2016: discrimination, psychological distress, and post-traumatic stress. Potential mediators included actigraphy-assessed sleep duration and efficiency from 2018. Cognitive battery and clinical adjudication in 2019 assessed cognitive function and clinically-adjudicated outcomes. Causal mediation analysis estimated the direct effect between stress burden and cognitive outcomes, and indirect effects through sleep, after adjusting for socio-demographics and hypertension. Higher stress burden had a significant direct effect on lower executive functioning and visuospatial performance. However, there were no significant indirect effects (i.e., mediation) by sleep disturbances on any domain of cognitive function assessed. Also, there were no significant direct or indirect effects on clinically-adjudicated outcomes. Multiple stressors often co-occur and may contribute to racial disparities in cognitive health. Findings suggest that higher stress burden had negative effects on functioning in executive and visuospatial domains in this community-based sample of older Black American adults. However, there was no evidence of mediation by sleep. Findings highlight the importance of continued work to identify modifiable pathways between stress burden and cognitive health disparities.

A network model of stress contagion: evidence from the vocational classroom

PurposeOur study focuses on stress contagion in vocational school classes, examining how students’ stress experiences affect their spatial classmates. For this purpose, we apply a novel formal network model that allows us to differentiate between endogenous and exogenous peer effects in the stress contagion process. Using the network model, we investigate effects on students’ stress levels, considering the stress and coping experiences of spatial peers as well as didactic-methodological context factors.MethodsWe test our statistical model using secondary data collected in a study involving 53 students from two classes at a public German vocational training school. The students’ experiences of stress (time pressure, pressure to succeed) and coping strategies (understanding of the subject matter, self-confidence) were captured using the experience sampling method, while classroom characteristics (e.g., teacher instruction, cooperative work) were recorded through video-based analysis of lessons. Utilizing the panel data, we employ maximum likelihood estimation to assess the spatial peer effects model for both classrooms.ResultsAmong other findings, all model specifications revealed significant peer effects for both stress measures, indicating that the higher the stress experience of immediate peers in the classroom, the higher the individual stress experienced by the students. Concerning the considered context factors, we found, for example, that increased cooperative work leads to higher levels of stress experience.ConclusionFrom a substantive perspective, our results underscore the role of peer-to-peer contagion in the vocational classroom and thus suggest a nuanced examination of cooperative practices. From a research methodology perspective, our approach illustrates how various methods (such as experience sampling, video-based classroom observation, and spatial network information) complement and enrich each other, highlighting the added value of our network analytical approach

Interpass temperature strategies for compressive residual stresses in cladding low-transformation-temperature material 16Cr8Ni via wire arc additive manufacturing

Low-transformation-temperature (LTT) materials induce compressive residual stresses within an effective depth in wire arc additive manufacturing (WAAM), which is well suited for cladding applications. However, this role was found to be sensitive to the phase transformation sequence. We developed an LTT material—16Cr8Ni (16Cr8Ni-LTT)—and to explore its potential, it was clad onto a KA36 substrate via WAAM. Three different interpass temperature strategies were designed as follows: (1) no interpass temperature control; (2) interpass temperature controlled at approximately 270 °C above the martensite transformation start temperature (Ms = 200 °C) for simultaneous phase transformation; and (3) interpass temperature controlled at approximately 50 °C below the martensite transformation finish temperature (Mf = 60 °C) for sequential phase transformation. A thermal elastic-plastic numerical model considering the phase transformation-induced plasticity (TRIP) was developed using in-house software, JWRAIN-Hybrid, to reproduce the temperature field and residual stresses of 16Cr8Ni-LTT cladding. X-ray diffraction (XRD) and contour methods were employed for residual stress measurements. The high accuracy of the model was validated in terms of the temperature, deformation, and residual stress. The reproduced maximum historical temperature distributions were combined with hardness tests to identify the depths and temperatures of the heat-affected zone (HAZ). The measured maximum compressive residual stresses induced by the three interpass temperature strategies for cladding 16Cr8Ni-LTT were −503, −420, and −720 MPa. Moreover, irrespective of the adopted interpass temperature strategy, both longitudinal and transverse residual stresses were compressive in the 16Cr8Ni-LTT cladding. This study provides scientific insights into LLT-induced compressive residual stresses and highlights the superiority and flexibility of cladding LTT materials via WAAM for improving the resistance of large metal components to fatigue and corrosion.

Predictive enrichment for the need of renal replacement in sepsis-associated acute kidney injury: combination of furosemide stress test and urinary biomarkers TIMP-2 and IGFBP-7

BackgroundIn sepsis, initial resuscitation with fluids is followed by efforts to achieve a negative fluid balance. However, patients with sepsis-associated acute kidney injury (SA-AKI) often need diuretic or renal replacement therapy (RRT). The dilemma is to predict whether early RRT might be advantageous or diuretics will suffice. Both the Furosemide Stress Test (FST) and measurements of the urinary biomarkers TIMP-2*IGFBP-7, if applied solely, do not provide sufficient guidance. We tested the hypothesis that a combination of two tests, i.e., an upstream FST combined with downstream measurements of urinary TIMP-2*IGFBP-7 concentrations improves the accuracy in predicting RRT necessity.MethodsIn this prospective, multicenter study 100 patients with sepsis (diagnosed < 48h), AKI stage ≥ 2, and an indication for negative fluid balance were included between 02/2020 and 12/2022. All patients received a standardized FST and urinary biomarkers TIMP-2*IGFBP-7 were serially measured immediately before and up to 12 h after the FST. The primary outcome was the RRT requirement within 7 days after inclusion.Results32% (n = 32/99) of SA-AKI patients eventually required RRT within 7 days. With the FST, urine TIMP-2*IGFBP-7 decreased within 2 h from 3.26 ng2/mL2/1000 (IQR: 1.38–5.53) to 2.36 ng2/mL2/1000 (IQR: 1.61–4.87) in RRT and 1.68 ng2/mL2/1000 (IQR: 0.56–2.94) to 0.27 ng2/mL2/1000 (IQR: 0.12–0.89) and non-RRT patients, respectively. While TIMP-2*IGFBP-7 concentrations remained low for up to 12 h in non-RRT patients, we noted a rebound in RRT patients after 6 h. TIMP-2*IGFBP-7 before FST (accuracy 0.66; 95%-CI 0.55–0.78) and the FST itself (accuracy 0.74; 95%-CI: 0.64–0.82) yielded moderate test accuracies in predicting RRT requirement. In contrast, a two-step approach, utilizing FST as an upstream screening tool followed by TIMP-2*IGFBP-7 quantification after 2 h improved predictive accuracy (0.83; 95%-CI 0.74–0.90, p = 0.03) compared to the FST alone, resulting in a positive predictive value of 0.86 (95%-CI 0.64–0.97), and a specificity of 0.96 (95%-CI 0.88–0.99).ConclusionsThe combined application of an upstream FST followed by urinary TIMP-2*IGFBP-7 measurements supports highly specific identification of SA-AKI patients requiring RRT. Upcoming interventional trials should elucidate if this high-risk SA-AKI subgroup, identified by our predictive enrichment approach, benefits from an early RRT initiation.

Treatment of type 2 diabetes and stress using neuro-emotional technique: case report

This case report presents a novel, non-pharmacological treatment of Type 2 Diabetes in a 46-year-old male, demonstrating improvements in blood chemistry and psychometric markers after 8 treatments using a Mind-Body Intervention (MBI) called Neuro-Emotional Technique (NET). The patient presented with a diagnosis of Type 2 Diabetes (T2D), pain, psychosocial indicators of stress and anxiety, and a score of 4 on the ACE-Q (Adverse Childhood Experiences Questionnaire) that is consistent with a predisposition to chronic disease and autoimmune disorders. Glucose levels for this patient were above normal levels (typically between 10-15mmol/L where optimal range is between 4-10mmol/L) for at least two months prior to the 4-week NET intervention period, despite the standard use of conventional antidiabetic medications (insulin injections). The patient exhibited numerous indictors of chronic stress that were hypothesised to be underlying his medical diagnosis and a series of 8 NET treatments over a period of 4 weeks was recommended. Psychometric tests and glucose measurements were recorded at baseline (prior to treatment), 4 weeks (at the conclusion of treatment) and at 8 weeks (4 weeks following the conclusion of treatment). Results show that glucose levels were reduced, and self-reported measures of depression, anxiety, stress, distress and pain all decreased from high and extreme levels to within normal ranges after 4 weeks, with ongoing improvement at 8 weeks. McEwen described the concept of allostatic load and the disruptive effects that cumulative stress can have on both mental and physical health. It is hypothesized that NET reduces allostatic load thereby fortifying homeostasis and the salutogenic stress response mechanisms involved in recovery from chronic illness, possibly via the Psycho-Immune-Neuroendocrine (PINE) network. Further studies with larger sample sizes are required to establish whether these results could be extrapolated to a wider population, however the results of this case suggest that it may be beneficial to consider co-management of T2D with an MBI such as NET.

Procrastination and Health in Nurses: Investigating the Roles of Stress, Health Behaviours and Social Support

Objectives: Evidence linking chronic procrastination to a range of poor health outcomes and trajectories continues to build. Yet, much of this research has been conducted in academic contexts or in non-student samples. Despite theory indicating that high-stress contexts increase vulnerability for procrastination, the pathways linking chronic procrastination to health outcomes proposed by the procrastination–health model have not been examined in a high stress environment. Accordingly, we tested the contribution of procrastination to health in nurses and whether social support was a protective factor. Design: Pre-registered cross-sectional study using a random sample of nurses recruited from the membership of a regional nursing association, supplemented by nurses and nurse trainees recruited from online nursing associations, conferences and forums. Methods: Nurses and nurse trainees (N = 597) completed measures of chronic procrastination, stress, health behaviours, social support and self-rated health. Results: Chronic procrastination was associated with perceived stress, health behaviours, self-rated health and social support in the expected directions. Consistent with the procrastination–health model, structural equation modelling revealed significant indirect effects linking chronic procrastination to poor self-rated health through higher stress and fewer health behaviours. Contrary to our hypotheses, social support did not moderate these pathways. Conclusions: This study is the first to demonstrate the relevance of procrastination for health in high-stress, non-academic contexts and to find support for both the stress and behavioural pathways linking procrastination to poor health outcomes. Findings further highlight the importance of addressing chronic procrastination as a vulnerability factor for poor health in nurses.

Measurement of stress characteristics in flexible multi-thin-film substrates using tomography common optical path interferometry

Flexible displays are lightweight, thin, flexible, and impact-resistant, making them a major trend in the display industry. However, their short lifespan is a significant challenge. Thus, improving process parameters and extending product life through advanced measurement technology is crucial. Therefore, this study proposes using tomography common optical path interferometry (TCOPI) to measure and analyze the tomography stress characteristics of multi-thin-film flexible substrates in the folding direction. For polyethylene terephthalate (PET) and polyimide (PI), three thicknesses of indium tin oxide (ITO) were coated on the PET, graphene/PET, and graphene/PI flexible film substrates, and an automatic sliding-folding test platform was used to fold each flexible film substrate. Moreover, this study used TCOPI to measure and analyze the change in residual stress and the sheet resistance of the samples before and after folding. Additionally, this study conducted inward and outward folding tests on graphene/PET/graphene and graphene/PI/graphene substrates to analyze tomographic stress and sheet resistance in the folding direction. After 11,000 folding tests, the graphene/PET/graphene sample exhibited smaller stress and sheet resistance change rates compared to the graphene/PI/graphene sample, as measured by TCOPI. Consequently, the graphene/PET/graphene sample demonstrated better resistance to folding stress in this batch manufacturing process. Specifically, for the graphene layer of the graphene/PET/graphene sample, the minimum absolute value of the stress change rate before and after folding reached 5%. At the same time, the minimum sheet resistance value change rate was 8%. Based on the tomography stress measurements, suggestions for fine-tuning process parameters are provided to improve the flexible film substrate’s folding endurance.

Bolt axial stress measurement method based on ultrasound multi-feature fusion

ABSTRACT There exist issues of ill-adaptability and low-accuracy in ultrasonic bolt stress measurement based on acoustic elasticity and scattering attenuation. Therefore, a bolt axial stress measurement method based on ultrasound multi-feature fusion is proposed in this paper. Firstly, ultrasound characteristic parameters based on the theory of acoustic elasticity are extracted, including the difference of time-of-flight (TOF) and the longitudinal wave attenuation coefficient in polycrystalline materials within the Rayleigh scattering range. Then, following the dimension reduction principle, the difference of TOF, attenuation coefficient, and clamping length are chosen as the input feature vectors for the fusion model. Subsequently, the SVR model is employed to establish a multi-feature fusion model for bolt axial stress measurement. Calibration and measurement experiments are conducted using the constructed experimental platform. Comparative analysis between the traditional measurement methods and the proposed method reveals that the SVR model can adapt to measurements of bolts with different lengths, overcoming the limitations of traditional methods. Furthermore, the measurement results of different fusion algorithms show that SVR yields significantly lower average relative errors in the measurement of the three bolts (3.35%, 4.44%, and 4.48%) compared to BP and RBF algorithms. This indicates higher measurement accuracy and immunity to low-stress effects.

Prenatal stress and gestational epigenetic age: No evidence of associations based on a large prospective multi-cohort study.

Psychological stress during pregnancy is known to have a range of long-lasting negative consequences on the development and health of offspring. Here, we tested whether a measure of prenatal early-life stress was associated with a biomarker of physiological development at birth, namely epigenetic gestational age, using foetal cord-blood DNA-methylation data. Longitudinal cohorts from the Netherlands (Generation R Study [Generation R], n = 1,396), the UK (British Avon Longitudinal Study of Parents and Children [ALSPAC], n = 642), and Norway (Mother, Father and Child Cohort Study [MoBa], n1 = 1,212 and n2 = 678) provided data on prenatal maternal stress and genome-wide DNA methylation from cord blood and were meta-analysed (pooled n = 3,928). Measures of epigenetic age acceleration were calculated using three different gestational epigenetic clocks: "Bohlin", "EPIC overlap" and "Knight". Prenatal stress exposure, examined as an overall cumulative score, was not significantly associated with epigenetically-estimated gestational age acceleration or deceleration in any of the clocks, based on the results of the pooled meta-analysis or those of the individual cohorts. No significant associations were identified with specific domains of prenatal stress exposure, including negative life events, contextual (socio-economic) stressors, parental risks (e.g., maternal psychopathology) and interpersonal risks (e.g., family conflict). Further, no significant associations were identified when analyses were stratified by sex. Overall, we find little support that prenatal psychosocial stress is associated with variation in epigenetic age at birth within the general paediatric population.

Direct Losses and Media Exposure to Death: The Long-Term Effect of Mourning during the COVID-19 Pandemic.

Background: The social distancing policies adopted during the COVID-19 pandemic forced many individuals to confront their mortality and worry about losing loved ones, making it impossible to say goodbye to them properly. Those not directly experiencing loss were inundated with information about COVID-19-related deaths throughout social media, leading to vicarious grief. This study delved into the long-term effects of direct and vicarious mourning on people's mental health during the COVID-19 pandemic. Method: A sample of 171 adults (65% female) aged 19-66 years (Mage = 25.8, SD = 8.57) voluntarily participated in an online survey assessing self-reported psychological measures of complicated grief, stress, depression, dispositional neuroticism, trait anxiety, and situational anxiety. Results: MANOVAs revealed that direct mourning experiences had an extremely severe impact on anxiety, stress, and fear of COVID-19, and a moderate effect on those without personal losses. Indeed, participants reporting high media exposure showed higher scores of depression and stress. Conclusions: Findings from the current study displayed that during the COVID-19 pandemic, people engaged more in proximal defenses than distal ones, taking health-protective measures, experiencing increased anxiety levels toward virus infection, and feeling distressed. Additionally, vicarious mourning was more strongly associated with depression due to emotional empathy with others.

P-094 Genetic variations of antioxidant genes may coordinate with lifestyle to define male fertility: A cross-sectional study with control group

Abstract Study question Are polymorphisms of the oxidative stress-related genes different between infertile men and reproductively healthy men? Does lifestyle contribute to fertility outcomes? Summary answer A strong association between the polymorphisms of two antioxidant genes and sperm quality was identified. The infertile men had higher BMI and alcohol usage. What is known already Antioxidant genes including catalase (CAT), superoxide dismutase (SOD), and nitric oxide synthase (NOS) play a critical role in spermatogenesis and sperm functions, especially in stress-response cell signaling pathways. Polymorphisms of antioxidant genes have been reported to be associated with gene function, hence sperm quality, ultimately affecting male fertility in various cohorts. Body mass index (BMI) is universally considered a marker of health reflecting whole health, nutrition, and lifestyle. Smoking and alcohol consumption expose the body to harmful chemicals and oxidative stress. Here, we examined the polymorphisms of antioxidant genes in Vietnamese men and the link with lifestyle to regulate fertility. Study design, size, duration This cross-sectional study with control group recruited from Hanoi Medical University Hospital from 2018-2020. The participants were divided into two groups regarding semen analysis based on WHO 2010 criteria. The participants gave consent, then were clinically examined, interviewed for lifestyle, and genetically analyzed for antioxidant gene variations. An extra inclusion criterion for the control men was having at least one healthy baby. The exclusion criteria were chromosome aberrations and Y chromosome microdeletion. Participants/materials, setting, methods The control group (N = 85) and infertile group (N = 107) were recruited. Semen analysis was carried out by the CASA instrument based on WHO 2010 criteria. Lifestyle characteristics including exercise (per week), smoking (yes/no), alcohol consumption (the threshold for “yes” was five standard drinks/occasion, at least one occasion/month) were recorded. Four variants SOD1:7958G&amp;gt;A, SOD2:c.47T&amp;gt;C, CAT:-262C&amp;gt;T, and NOS3:-786C&amp;gt;T were analyzed by Sanger sequencing. Pearson’s, Chi-squared (χ2), Wilcoxon rank-sum, ANOVA test, and Bonferroni correction were applied where specifically required. Main results and the role of chance The average age of the two groups was not different (31.96 ± 4.87 vs 31.93 ± 6.3 years old, p = 0.92). The body mass index (BMI) of infertile men was significantly higher than the control (24.84 ± 2.31 vs 23.53 ± 2.55 kg/m2, p = 0.00026). No significant difference was identified regarding exercise and smoking. Notably, 96.26% of infertile men consumed alcohol in comparison with 88.23% of the control (p = 0,034). Genetically, SOD1:7958GA genotype and NOS3:-786CT genotype in the infertility group were significantly higher than in the control, OR = 2.191 (95% CI: 1.226–3.915, p = 0.004) and OR = 3.135 (95% CI: 1.591–6.180, p &amp;lt; 0.001), respectively. The frequency of the SOD2:c.47TC genotype was significantly higher in the male infertility group than in the control (OR = 1.941, 95% CI: 1.063–3.595, p = 0.029). Individuals carrying the SOD1:GA+AA, SOD2:TC+CC, and CAT:CT/TT genotype pattern had an increased risk of male infertility (OR = 7.614, p = 0.007), suggesting that gene-gene interaction plays an important role in male reproduction. To our knowledge, this is the first report worldwide on the association between the SOD1:7958G&amp;gt;A polymorphism and male infertility. Together, the data suggest that antioxidant gene polymorphisms and lifestyle, especially alcohol usage, coordinate to regulate male fertility. Limitations, reasons for caution Direct measurement of oxidative stress in the semen of men having different gene variations would be required to ascertain the link between the polymorphisms of antioxidant genes and fertility. The sample size was relatively small, a larger sample size would be required to accurately represent the Vietnamese population. Wider implications of the findings The findings can assist in infertility diagnosis focusing on the group of higher infertile risk genetic variations. Interventions such as antioxidant supplements would be developed and applied, lifestyle advice would be given specifically for a group having high-risk gene variations to accurately target fertility issues. Trial registration number Not applicable

P-482 Construct validity of the donor conception identity questionnaire and its association with searching for and finding donor connections

Abstract Study question Is there an association between donor conception identity, psychological and social wellbeing, and searching for and finding donor connections? Summary answer Donor conceived individuals differ on key domains that tap into aspects of their donor conception identity. These relate to searching for and finding donor connections. What is known already There is variation in the ways that donor conceived individuals feel about their conception. Factors such as the age of disclosure are associated with how donor conceived individuals feel about being donor conceived. Associations have been found between donor conception identity and parent-child relationship quality among adolescents, suggesting that curiosity and negativity about donor conception are influenced by the valence of the parent-child relationship. The Donor Conception Identity Questionnaire (DCIQ) has not been previously validated. Study design, size, duration 88 donor conceived adults in the UK participated in a cross-sectional study using an online multi-method survey. Data were collected between January and August 2022. The survey was advertised by the Donor Conception Network and Donor Conceived Register Registrants’ Panel via mailing lists and social media and circulated by the research team and others on social media and university mailing lists. The present findings draw from a subsection of the data collected from this survey. Participants/materials, setting, methods Most participants identified as female (n = 65,74%) and were conceived using donor sperm (n = 79,90%). Participants completed the 25-item DCIQ (adapted from studies of donor conception and adoption identity), and the Mental Health Continuum, the Satisfaction with Life Scale, and adapted versions of the community connectedness and pride subscales of the Gender Minority Stress and Resilience Measure and the HIV Stigma Scale. Participants reported on whether they had searched for and/or found donor connections. Main results and the role of chance Factor analysis of items on the DCIQ identified four domains: 1. Concern and centrality, 2. Internalised stigma, 3. Pride and acceptance, and 4. Openness and understanding. ‘Concern and centrality’ correlated positively with community connectedness (r(86)= 283, p=.008), and stigma (r(86)= 280, p= .008), and negatively with pride (r(86) =-.398, p&amp;lt;.001). ‘Internalised stigma’ was positively correlated with stigma (r(86)=.858, p&amp;lt;.001) and negatively correlated with psychological wellbeing (r(85)=-.378, p &amp;lt;.001), satisfaction with life (r(86)= -.263, p=.013) and pride (r(86)=-716, p&amp;lt;.001). ‘Pride and acceptance’ was positively correlated with psychological wellbeing (r(85) = .276, p = .010), satisfaction with life (r(86)=.329, p =.002) and pride (r(86)=.800, p&amp;lt;.001), and negatively correlated with stigma (r(86)=-.396, p &amp;lt;.001). ‘Openness and understanding’ was positively correlated with psychological wellbeing (r(85)=.304, p=.004), satisfaction with life (r(86)=.316, p=.003), and pride (r(86)=.584, p&amp;lt;.001) and negatively correlated with stigma (r(86)=-.614, p&amp;lt;.001). Searchers scored higher than non-searchers on ‘concern and centrality’ F(1, 79) = 7.543, p= .007 and ‘internalised stigma’ (F(1, 79)=4.355, p=.040). Donor conceived individuals who had found donor connections scored lower on ‘internalised stigma’ F(1, 79) = 7.071, p=.009 and higher on ‘openness and understanding’ (F(1, 79)=6.083, p=0.016) compared to those who had not found their donor connections. Limitations, reasons for caution Lack of diversity within the sample with regards to participant gender, age, and donation type means that findings may not apply across donor conceived populations. The sample were self-selected. Causal relationships between variables cannot be established. Wider implications of the findings The DCIQ may be useful to understand variation in donor conceived people’s psychological health and interest in their donor. The value of the measure in a practical context (e.g., in counselling) should now be evaluated. Further work to validate the scale across different contexts and with different populations is recommended. Trial registration number the UK Economic and Social Research Council [New Investigator Award ES/S015426/1

Determinants of the inheritance behaviors among FELDA communities in Malaysia: A study using the PLS-SEM approach

This study aims to identify the impact of inheritance literacy, inheritance socialization, inheritance stress, and peer influence on the inheritance behaviors among FELDA communities in Malaysia. Inheritance literacy pertains to individuals’ comprehension of wealth transfer and estate planning, while peer influencer evaluates friends’ impact on inheritance attitudes; inheritance socialization explores family interactions’ role in shaping inheritance attitudes, and inheritance stress measures emotional strain in inheritance matters, with inheritance behaviors encompassing asset management and wealth transfer decisions for future generations by individuals and families. Understanding inheritance behaviors is crucial, as it helps individuals depict their inheritance knowledge and attitudes toward FELDA inheritance better, fostering a more favorable inheritance attitude. Through self-administered survey questionnaires, data related to FELDA communities are obtained using convenience sampling from 413 respondents. This study applies Partial Least Squares Structural Equation Modeling (PLS-SEM) technique to test the research hypotheses. The present study’s outcome confirms that two determinants, which are inheritance literacy and inheritance socialization significantly influence the inheritance behavior of FELDA communities. However, inheritance stress and peer influence determinants have statistically insignificant influence inheritance behavior. This study’s theoretical framework enriches the discussions on wealth management and financial behavior by refining and expanding upon existing financial behavior theories to incorporate inheritance-specific behaviors. The present study is exclusive in its effort to ascertain the relative importance of both inheritance behavior and the FELDA communities. This paper will assist the government, inheritance service providers, and policymakers in offering innovative economic schemes and designing policies that may enhance the inheritance behavior wellbeing of FELDA communities. This article also provides a roadmap to guide future research in this area.

Optimizing guided wave propagation for sensitive axial stress measurement in steel pipes

Steel pipe structures are commonly used in the industrial field. Stress-induced structural failures can have a significant impact on equipment safety. Therefore, effective stress monitoring is a crucial area of research. Constrained by the multi-frequency and multi-modal characteristics of ultrasonic guided waves, the limitations of traditional stress measurement methods based on these waves lie in the lack of a systematic analysis of the impact of multi-modal fused signals on stress measurement. To explore the optimal guided wave stress measurement strategy, a mathematical model for the propagation of longitudinal guided waves in prestressed steel pipes, based on the principles of acoustoelasticity, is proposed. Using this model, the sensitivity of stress to each sub-mode of the longitudinal guided waves is analyzed, leading to the identification of the optimal guided wave mode (L(0,2) mode) and frequency range. In order to avoid the excitation of other low-sensitivity modes, further analysis of the optimal parameters for the piezoelectric array is conducted. Simulation results indicate that the designed transducer can effectively excite the target mode with high purity. The stress sensitivity of the target mode was experimentally determined to be −2.5×10−5MPa−1, which closely aligns with the theoretical results. Comparative analysis of the experiments emphasizes the influence of modal control on measurement outcomes. By selecting and controlling the appropriate mode, the maximum relative error in stress measurement is observed to be 5%.