Streptozotocin-induced Diabetes Mellitus Rats Research Articles

Phytochemical Profile and Antidiabetic, Antioxidant, and Anti-Inflammatory Activities of Gypsophila paniculata Ethanol Extract in Rat Streptozotocin-Induced Diabetes Mellitus.

The present study aimed to investigate the effects of the Gypsophila paniculata ethanol extract (GPEE) on oxidative stress, inflammation, and metabolic markers in a rat model of streptozotocin-induced diabetes mellitus (DM). Phytochemical analysis using high-performance liquid chromatography coupled with mass spectrometry was performed to measure the total phenolic and flavonoid contents. In vitro antioxidant activity was evaluated through DPPH, FRAP, H2O2, and NO scavenging tests, and the in vivo effects of the GPEE were assessed in streptozotocin-induced DM rats. Treatments with the GPEE, metformin, and Trolox were administrated by gavage for 10 days. On day 11, blood was collected, and serum oxidative stress (total oxidative status, oxidative stress index, malondialdehyde, advanced oxidation protein products, 8-hydroxydeoxyguanosine, nitric oxide, 3-nitrotyrosine, advanced glycation end-products, total antioxidant reactivity, total thiols), inflammatory (IL-1β, NF-κB, IL-18, and gasdermin D), metabolic (fasting glucose, total cholesterol, triglycerides, and triglyceride-glucose index), and liver injury (AST, ALT, and AST:ALT ratio) markers were measured. The GPEE was found to have a significant polyphenols content and a moderate in vitro antioxidant effect. In vivo, the GPEE lowered oxidants and increased antioxidants, decreased inflammatory markers and blood glucose, and improved lipid profiles and transaminases in a dose-dependent manner, with higher doses having a better effect, being comparable to those of metformin and Trolox.

Role of serotonin on pituitary-thyroid functions and antioxidants in diabetic male rats

Diabetes mellitus causes an increase in oxidative stress. Serotonin is a hormone its level in circulation reduced in diabetes; recently, it has been found to possess antioxidant qualities. The purpose of the study was to look at the impact of diabetes on pituitary-thyroid functioning, and serotonin role in modulating these effects in a streptozotocin-induced diabetes mellitus rat model. The thirty-tow healthy male rats with a body weight of 180-200 gm were split equally into 4 groups, group [1[: control, group [2]: Serotonin, group [3]: diabetic, and group [4]: Serotonin-diabetic. The rats were weighed and serum levels of glucose, insulin, malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, serotonin, thyroid stimulating hormone, thyroxine, and triiodothyronine were measured. Our results presented that there was a significant increase in T3, T4, glucose, and MDA in the diabetic group compared to the control, while a significant decrease in T3, and T4, glucose, and MDA in the Serotonin with diabetic group compared to diabetic group. Furthermore, a significant decrease in TSH, serotonin, insulin and GPX, CAT, and SOD antioxidants in the diabetic group compared to control, while a significant increase in serum TSH, serotonin, insulin and CAT, GPX and SOD antioxidants in the Serotonin+diabetic group compared to diabetic group as well as a significant increase in serum insulin in the Serotonin group compared to all other groups. Serotonin plays a protective role against diabetes-induced and pituitary-thyroid disorder caused by diabetes which might be because of its antioxidant qualities and ability to maintain glucose and insulin balance.

Anti-Diabetic Effects of Pomegranate Peel Extract and L-Carnitine on Streptozotocin Induced Diabetes In Rats

Type 2 diabetes mellitus is a far reaching ongoing metabolic problem portrayed by hyperglycemia and related with a few intricacies like hyperlipidemia. The current study aimed to study the anti-diabetic efficacy of pomegranate peel extract and L-carnitine on streptozotocin-induced diabetes mellitus in rats. A total of 70 male rats were divided into 7 groups (normal rats treated with the pomegranate peel extract and L-carnitine; rats given a high-fat diet to cause hyperlipidemia, this rats given low-dose intraperitoneal streptozotocin injections to cause type II diabetes; diabetic rats given PPE and L-carnitine orally every day for 12 weeks. Measurements of body mass, blood sugar, lipid profile and antioxidant enzyme activity were made.Results: The treatment group that received PPE + L-carnitine showed a significant decrease in weight, blood glucose, cholesterol, triglycerides and low-density lipoprotein were significantly reduced, while high-density lipoprotein levels were significantly increased. Superoxide dismutase levels were increased, catalase and Nuclear Factor Erythroid-derived 2 (Nfe2) in diabetic rats treated with PPE and L-carnitine, while Malondialdehyde levels decreased significantly. According to the results of the study, PPE and L-carnitine had significant anti-hyperglycemic, hypolipidemic, and antioxidant benefits after 12 weeks of treatment in streptozotocin-induced diabetic rats.

Impacts of trelagliptin and remogliflozin alone and in combination with Alpha Lipoic Acid on cardiac function in streptozotocin-induced diabetes mellitusinrats.

This study investigated the effects of trelagliptin and remogliflozin, alone and in combination with alpha lipoic acid (ALA), on cardiac biomarkers in diabetic cardiomyopathy (DCM). We aimed to assess the management of glucotoxicity consequences in streptozotocin-induced diabetic rats by measuring serum levels of pharmacologically active endogenous ligands. Forty-eight male rats were divided into different treatment groups, including negative control, positive control, and four experimental groups. After inducing diabetes, the rats were treated for 28 days, and serum levels of biomarkers associated with oxidative stress (malondialdehyde and thioredoxin-interacting protein), inflammation (nuclear factor NF-kappa-B p105 and lipoprotein-associated phospholipase A2), and myopathy (neprilysin and high selective cardiac troponin T) were measured. Immunohistochemical analysis of heart cells was also performed. The results showed that inducing hyperglycemia increased serum glucose levels and biomarkers associated with DCM. However, all treatment groups exhibited a significant decrease in these biomarkers and an increase in insulin levels compared to the diabetic control group. The groups receiving combination therapy with ALA showed greater improvements in cardiac biomarkers compared to the individual treatments. The immunohistochemical analysis supported these findings by demonstrating a reduction in the percentage area of cathepsin B, a protein involved in DCM pathophysiology. In conclusion, supplementing the base treatments with ALA showed promise in enhancing cardiac biomarkers associated with DCM. The combination of trelagliptin, remogliflozin, and ALA may have additional clinical value in managing DCM by targeting oxidative stress, inflammation, and glucotoxicity. However, further research is needed to validate these findings and explore their potential clinical applications.

Ameliorative Effect of Combined Treatment of Blueberry Fruit Extract and Voglibose on Streptozotocin-Induced Diabetes Mellitus in Rats

Ameliorative Effect of Combined Treatment of Blueberry Fruit Extract and Voglibose on Streptozotocin-Induced Diabetes Mellitus in Rats

Green decorated gold nanoparticles on magnetic nanoparticles mediated by Calendula extract for the study of preventive effects in streptozotocin-induced gestational diabetes mellitus rats

Green decorated gold nanoparticles on magnetic nanoparticles mediated by Calendula extract for the study of preventive effects in streptozotocin-induced gestational diabetes mellitus rats

Effect of near-infrared laser treatment on improving erectile function in rats with diabetes mellitus.

Diabetes mellitus-induced erectile dysfunction is difficult to treat. The oxidative stress created by diabetes mellitus is a major cause of injuries to the corpus cavernosum, thereby resulting in erectile dysfunction. Near-infrared laser has already been shown to be effective in treating multiple brain disorders because of its antioxidative stress effect. To investigate whether a near-infrared laser improves the erectile function of diabetes mellitus-induced erectile dysfunction rats through its antioxidative stress effect. Knowing its advantage of reasonable deep tissue penetration and good photoactivation on mitochondria, a near-infrared laser with wavelength of 808nm was used in the experiment. Since the internal and external corpus cavernosum were covered by different tissue layers, the laser penetration rates of the internal and external corpus cavernosum were measured separately. Different radiant exposure settings were applied: in the initial experiment, 40 male Sprague-Dawley rats were randomly assigned to five groups, normal controls, and streptozotocin-induced diabetes mellitus rats that 10weeks later received various radiant exposures (J/cm2 ) from the near-infrared laser (DM0J(DM+NIR 0 J/cm2 ), DM1J, DM2J, and DM4J) in the subsequent 2weeks. Erectile function was then assessed 1week after near-infrared treatment. It was found that the initial radiant exposure setting was not optimal according to the Arndt-Schulz rule. We performed a second experiment using a different radiant exposure setting. Forty male rats were randomly divided into five groups (normal controls, DM0J, DM4J, DM8J, and DM16J), and the near-infrared laser was again applied according to the new setting, and erectile function was assessed as in the first experiment. Histologic, biochemical, and proteomic analyses were then conducted. Recovery of erectile function of varying degrees was observed in the near-infrared treatment groups, and radiant exposure of 4 J/cm2 achieved optimal results. The DM4J group showed improvement in mitochondrial function and morphology in diabetes mellitus rats, and it was found that oxidative stress levels were significantly reduced by near-infrared exposure. The tissue structure of the corpus cavernosum was also improved by near-infrared exposure. The proteomics analysis confirming multiple biologic processes were changed by diabetes mellitus and near-infrared. Near-infrared laser activated mitochondria, improved oxidative stress, repaired the damage to penile corpus cavernosum tissue structures caused by diabetes mellitus, and improved erectile function in diabetes mellitus rats. These results thus raise the possibility that human patients with diabetes mellitus-induced erectile dysfunction may respond to near-infrared therapy in a manner that parallels the responses we observed in animal study.

Berberine Suppresses Gestational Diabetes in Streptozotocin-induced Diabetes Mellitus Rats by Suppression of Inflammatory Mediators

Abstract: Background: Gestational diabetes mellitus (GDM) is a serious health condition witnessed among females who experience insulin resistance and glucose intolerance with the onset of pregnancy. Multiple risk factors prevail both to the gestating mother and the growing fetus at the time of pregnancy which may prolong even postpartum. Aim: Berberine, a natural plant extract known for its anti-inflammatory and potent antidiabetic activity was used to clinically suppress the risk factors involved in Gestational diabetes mellitus. Materials and Methods: Female Wistar rats were used as the models for this study. Streptozotocin was administered to induce diabetes in the female rat model. Berberine was administered to the test animals and monitored with regular analysis of body weight, fetal-placental weight and index, Foetal Blood Glucose (FBG), Serum advanced glycation end products (AGEs), and antioxidant enzyme concentrations. Biochemical parameters, lipids, and pro-inflammatory cytokine levels were assayed to study the influence of Berberine. Results: Upon investigating, it was observed that Berberine produced remarkable activity in suppressing glucose intolerance and insulin resistance by targeting multiple criteria, including inflammatory mediators enlisted above. Conclusion: From this study, it is evident that Berberine can be used as a therapeutic agent to treat gestational diabetes. Keywords: Gestational diabetes mellitus, Inflammation, Berberine, Antioxidant, Antidiabetic.

LC-MS-based metabolite profiling of aqueous extract of Pergularia tomentosa L. and its anti-hyperglycemic effect.

In this study, to find scientific evidence for the traditional use of Pergularia tomentosa as an anti-diabetic remedy, the effects of its aqueous extract on streptozotocin-induced diabetes mellitus in rats were evaluated. Wistar rats were fasted overnight and diabetes mellitus was induced using streptozotocin (50 mg/kg body weight). The rats were randomly and equally divided into four groups (n=5): group I (normoglycaemic control), group II (diabetic rats), group III (diabetic rats treated with 200 mg/kg BW of an aqueous extract of P. tomentosa), group IV (normoglycemic rats treated with 200 mg/kg BW of an aqueous extract of P. tomentosa). Chemical profiling of the aqueous extract was carried out using liquid chromatography coupled with electrospray ionization and multiple-stage linear ion-trap and orbitrap high-resolution mass spectrometry (LC-ESI/LTQOrbitrap/MS/MS). In addition, the quantitative determination of the main cardenolides in the extract was carried out by an analytical approach based on LC coupled to tandem mass spectrometry with ESI source and hybrid triple quadrupole-linear ion trap mass analyzer (LC-ESI/QTrap/MS/MS). Aqueous extract of P. tomentosa showed a reasonable reduction in blood glucose level. Probably, the P. tomentosa effect on hyperglycemic and hyperlipidemic diabetic animals was associated with antioxidant properties, triglyceride levels, as well as liver enzymes. Meanwhile, LC-ESI/LTQOrbitrap/MS/MS analysis led to identification of double-linked cardenolides along with cardenolides and flavone glycosides as the main bioactive compounds. The extract decreased the glucose level and induced a beneficial effect on the lipid profile, probably due to the presence of cardenolide glycosides.

Ketogenic diet modulates cardiac metabolic dysregulation in streptozocin-induced diabetic rats

The ketogenic diet (KD) might improve cardiac function in diabetic cardiomyopathy, but the mechanisms remain unclear. This study investigated the effects of KD on myocardial fatty acid (FA), glucose, and ketone metabolism in diabetic cardiomyopathy. Echocardiograms, biochemistry, and micro-positron emission tomography were performed to evaluate cardiac function and glucose uptake in control rats and streptozotocin-induced diabetes mellitus (DM) rats with normal diet (ND) or KD for 6 weeks. Histopathology, adenosine triphosphate measurement, and Western blot were performed in the ventricular myocytes to analyze fibrosis, FA, ketone body, and glucose utilization. The ND-fed DM rats exhibited impaired left ventricular systolic function and increased chamber dilatation, whereas control and KD-fed DM rats did not. The KD reduced myocardial fibrosis and apoptosis in the DM rats. Myocardial glucose uptake in the micro-positron emission tomography was similar between ND-fed DM rats and KD-fed DM rats and was substantially lower than the control rats. Compared with the control rats, ND-fed DM rats had increased phosphorylation of acetyl CoA carboxylase and higher expressions of CD-36, carnitine palmitoyltransferase-1β, tumor necrosis factor-α, interleukin-1β, interleukin6, PERK, and e-IF2α as well as more myocardial fibrosis and apoptosis (assessed by Bcl-2, BAX, and caspase-3 expression); these increases were attenuated in the KD-fed DM rats. Moreover, ND-fed DM rats had significantly lower myocardial adenosine triphosphate, BHB, and OXCT1 levels than the control and KD-fed DM rats. The KD may improve the condition of diabetic cardiomyopathy by suppressing FA metabolism, increasing ketone utilization, and decreasing endoplasmic reticulum stress and inflammation.

Extracellular vesicles derived from human umbilical cord mesenchymal stem cells relieves diabetic retinopathy through a microRNA-30c-5p-dependent mechanism

AimsExtracellular vesicle (EV)-transferred microRNAs (miRNAs) are proved to be potentially therapeutic candidates. Here, we attempted to unveil the role of delivery of miR-30c-5p by human umbilical cord mesenchymal stem cells (hUCMSCs)-derived EVs in diabetic retinopathy (DR). MethodsmiR-30c-5p and PLCG1 expression in streptozotocin-induced diabetes mellitus (DM) rats and high glucose (HG)-treated human retinal endothelial cells (HRECs) was quantified, followed by analysis on their interaction. EVs were isolated from hUCMSCs and co-cultured with HRECs. Through gain- and loss-of-function assays, the role of hUCMSCs-derived EV containing miR-30c-5p in DR involving PLCG1 and NF-κB pathway was analyzed in vitro and in vivo. ResultsElevated PLCG1 was found in DM rats and HG-treated HRECs where miR-30c-5p was reduced while increased in hUCMSC-derived EVs. PLCG1 was pinpointed as a target gene of miR-30c-5p, which consequently disrupted the PKC/NF-κB pathway. hUCMSC-derived EVs decreased inflammation reaction by transferring miR-30c-5p in DM rats and HG-treated HRECs. Furthermore, similar changing tendency was observed in HG-treated HRECs induced by overexpressed miR-30c-5p through downregulation of PLCG1 in vivo. ConclusionOverall, our findings underlined delivery of miR-30c-5p by hUCMSC-derived EVs as a novel suppressor in the inflammatory response following DR.

Sequential and Dynamic Variations of IL-6, CD18, ICAM, TNF-α, and Microstructure in the Early Stage of Diabetic Retinopathy.

Objective The purpose of this project is to make sequential and indepth observation of the variations of retinal microvascular, microstructure, and inflammatory mediators at the early stage of diabetic retinopathy (DR) in streptozotocin-induced diabetes mellitus (DM) rats. Methods DM was induced by a single intraperitoneal injection of 60 mg/kg body weight streptozotocin (STZ). The fluorescein fundus angiography, hematoxylin and eosin staining, periodic acid-Schiff staining, fluorescence imaging techniques, quantitative real-time PCR, and vascular endothelial growth factor- (VEGF-) A ELISA were performed on the 8th day, at the 4th week, 6th week, 8th week, and 10th week after DM induction, respectively. Results In this study, we observed not only the decrease of retinal ganglion cells (RGCs) and the increase of endotheliocytes to pericytes (E/P) ratio, acellular capillaries, and type IV collagen-positive strands began to occur on the 8th day after induction but the vascular permeability and new vessel buds began to appear in the diabetes group at the 8th week, while the expression of VEGF-A, VEGF mRNA, IL-6 mRNA, ICAM mRNA, and TNF-α mRNA were significantly higher in the diabetes group compared with the normal group(P < 0.01) on the 8th day after induction and maintained a high expression level throughout the 10-week observation period. However, the expression of CD18 mRNA began to increase significantly at the 4th week after induction and reached a peak at the 6th week. Conclusion Our study indicated the abnormal alterations of microvessels, microstructure, and inflammatory mediators at the early stage of DR, which confirms and supplements the previous research, and also promotes an indepth understanding and exploration of the pathophysiology and underlying pathogenesis of DR.

The effects of Origanum onites in streptozotocin-induced diabetes mellitus in rats

The effects of Origanum onites in streptozotocin-induced diabetes mellitus in rats

Astaxanthin-s-allyl cysteine diester against high glucose-induced neuronal toxicity in vitro and diabetes-associated cognitive decline in vivo: Effect on p53, oxidative stress and mitochondrial function

Neuroprotective effect of astaxanthin-s-allyl cysteine diester (AST-SAC) against high glucose (HG)-induced oxidative stress in in vitro and cognitive decline under diabetes conditions in in vivo has been explored. Pretreatment of AST-SAC (5, 10 and 15 μM) dose-dependently preserved the neuronal cells (SH-SY5Y) viability against HG toxicity through i) decreasing oxidative stress (decreasing reactive oxygen species generation and increasing endogenous antioxidants level); ii) protecting mitochondrial function [oxidative phosphorylation (OXPHOS) complexes activity and mitochondrial membrane potential (MMP)]; and iii) decreasing p53 level thereby subsequently decreasing the level of apoptotic marker proteins. Male Spraque-Dawley rats were orally administered AST-SAC (1 mg/kg/day) for 45 days in streptozotocin-induced diabetes mellitus (DM) rats. AST-SAC administration prevented the loss of spatial memory in DM rats as determined using the novel object location test. AST-SAC administration alleviated the DM-induced injury in brain such as increased cholinesterases activity, elevated oxidative stress and mitochondrial dysfunction. Altogether, the results from the present study demonstrated that AST-SAC averted the neuronal apoptosis and preserved the cognitive function against HG toxicity under DM conditions.

Pharmacokinetics and Pharmacodynamics of Curcumin-Loaded Solid Lipid Nanoparticles in the Management of Streptozotocin-Induced Diabetes Mellitus: Application of Central Composite Design

Due to poor bioavailability and chemical instability, the effectiveness of curcumin is negligible in the treatment of numerous diseases. Solid lipid nanoparticles (SLNs) increase the bioavailability of lipophilic compounds and protect the drug from gastrointestinal degradation. The objective of our study is the utilization of SLNs to improve the pharmacokinetics and pharmacodynamics of curcumin in the management of diabetes mellitus. Central composite design was used to prepare curcumin-loaded SLNs (Cur-SLN). The analysis of independent variables like drug concentration, lipid concentration, and surfactant concentration was carried out using analysis of variance (ANOVA) to obtain the optimized batch (optimized Cur-SLN) having the desired values of dependent variables particle size and entrapment efficiency. In vitro release, differential scanning calorimeter (DSC), transmission electron microscopy (TEM), and Fourier Transform Infra-Red (FTIR) studies of optimized Cur-SLN were carried out and then its pharmacokinetic and pharmacodynamic studies were performed. The model was found to be significant for particle size and entrapment efficiency based on F-value and p-value. The optimized batch's predicted values were in close agreement with the actual values of particle size and entrapment efficiency. TEM results confirm mono-dispersion and spherical shape of particles in the formulation. The DSC results confirmed the changing of drug from crystalline to amorphous form. Burst release followed by the sustained release was obtained in the in vitro release studies. The pharmacokinetic study shows enhanced bioavailability of optimized Cur-SLN compared with a plain drug suspension. The optimized Cur-SLN achieved higher antidiabetic activity in streptozotocin-induced diabetes mellitus rats than the plain drug suspension. SLNs can be used as a promising technique for delivering curcumin in the management of diabetes mellitus.

DIFFERENTIAL EXPRESSION OF VASCULAR DYSFUNCTION OF SMALLER DIAMETER ARTERIES IN A RODENT MODEL OF METABOLIC SYNDROME

Objective: Metabolic syndrome, driven by obesity and diabetes, is a major contributor to cardiovascular disease. While large arteries vascular dysfunction is a well-documented phenomenon of metabolic syndrome, vascular disease of smaller diameter arteries, which are key contributors to peripheral vascular resistance and blood pressure control, remains uncertain. Using in-vitro organ-bath preparation, this study, therefore, investigated functional responses of the superior mesenteric and right iliac arteries in a high fat diet (HFD)/streptozotocin-induced diabetes mellitus rat model. Design and method: Five-week-old male Wistar Albino rats (n = 24) were fed with either HFD (45 kcal% fat) or control diet (10 kcal% fat) for 10 weeks. At 10 weeks of age, 40 mg/kg streptozotocin and saline were injected intraperitoneally into the HFD and control groups, respectively. Results: Diabetic HFD rats displayed a time-dependent increase (p &lt; 0.01) in water intake, urine output, fasting blood glucose and tailcuff systolic blood pressure compared to controls (154 ± 6 vs. 110 ± 4 mmHg). Both mesenteric and iliac vasoconstrictor responses (N/g) to norepinephrine (1E-9 – 3E-5 M), but not to the depolarizing signals of high potassium (5 – 120 mM), were greater (p &lt; 0.01) in the HFD group relative to controls. Mesenteric, but not iliac, endothelium-dependent vasorelaxation to acetylcholine (1E-10 – 1E-5 M) was blunted (p &lt; 0.05) in the HFD rats compared with controls. In contrast, mesenteric and iliac endothelium-independent vasorelaxation responses to sodium nitroprusside (1E-11 – 1E-6 M) remained comparable between groups. Conclusions: Vascular functional responses in smaller diameter arteries are differentially expressed in metabolic syndrome, demonstrating upregulated vasoconstriction to adrenergic stimuli and/or impaired endothelium-dependent relaxation. These vascular abnormalities align with those previously described in larger arteries and could therefore further promote the development of cardiovascular disease in metabolic syndrome.

Effects of renal ischemia injury on brain in diabetic and non-diabetic rats: Role of angiotensin II type 2 receptor and angiotensin-converting enzyme 2

Clinically, patients with diabetes mellitus (DM) are more susceptible to ischemic renal injury (IRI) than non-diabetic (ND) patients. Besides, IRI predisposes distant organ dysfunctions including, neurological dysfunction, in which the major contributor remains renin-angiotensin system (RAS). Interestingly, the role of depressor arm of RAS on IRI-associated neurological sequalae remains unclear. Hence, this study aimed to delineate the role of angiotensin II type 2 receptor (AT2R) and angiotensin-converting enzyme 2 (ACE2) under the same. ND and Streptozotocin-induced DM rats with bilateral IRI were treated with AT2R agonist-Compound 21 (C21) (0.3 mg/kg/day, i.p.) or ACE2 activator-Diminazene Aceturate (Dize), (5 mg/kg/day, p.o.) either alone or as combination therapy. Effect of IRI on neurological functions were assessed by behavioural, biochemical, and histopathological analysis. Immunohistochemistry, ELISA and qRT-PCR experiments were conducted for evaluation of the molecular mechanisms. We found that in ND and DM rats, IRI causes increased hippocampal MDA and nitrite levels, augmented inflammatory cytokines (granulocyte-colony stimulating factor, glial fibrillary acidic protein), altered protein levels of Ang II, Ang-(1–7) and mRNA expressions of At1r, At2r and Masr. Treatment with C21 and Dize effectively normalised above-mentioned pathological alterations. Moreover, the protective effect of C21 and Dize combination therapy was better than respective monotherapies, and more likely, exerted via augmentation of protein and mRNA levels of depressor arm components. Thus, AT2R agonist and ACE2 activator therapy prevents the development of IRI-associated neurological dysfunction by attenuating oxidative stress and inflammation, upregulating depressor arm of RAS in brain under ND and DM conditions.

Assessment of the Antioxidant Potential of Hypoestes rosea Leafin Streptozotocin-induced Diabetic Albino Rats

Aims: The present paper was to investigate possible antioxidant actions of Hypoestes rosea leaf extracts in streptozotocin-induced diabetes mellitus in rats.&#x0D; Study Design: This study is an interventional study.&#x0D; Place and Duration of Study: This study was conducted in the animal house unit of the Department of Anatomy and physiology, University of Port Harcourt, Rivers State. between February, 2019 and September, 2019.&#x0D; Methodology: A total of hundred and sixteen (116) albino rats were assigned by weight into Eighteen (18) groups. The duration of the study was fifteen (15) days for acute and thirty (30) days sub-chronic. The study groups comprised of two (2) treatment phases each (prophylactic and therapeutic) with nine (9) experimental groups in each of the study groups. Ten (10) rats each were assigned to the two (2) positive control groups and six (6) rats each were assigned to the other groups. These groups of rats with an average body weight of 201±.65.20 to 232 ± 16.23 g were treated as follows: Healthy rats received de-ionized water (Negative Control); diabetic rats administered with de-ionized water (Positive Control); healthy rats received aqueous extract orally (EC 100 mg/kg body weight) and diabetic rats administered with aqueous extract orally, daily for fifteen (15) days and thirty (30) days (AEHR 100, 200 and 300 mg/kg body weight; Diabetic Treated group). Animals were fasted for 16 hrs, weighed and painlessly sacrificed and blood was collected through the jugular vein on day sixteen (16) and thirty-one (31) after the experimental phases. Blood sample was collected for the determination of SOD and TAC through colorimetric method.&#x0D; Results: Results showed that the plasma antioxidant enzymes (superoxide dismutase and total antioxidant capacity) activities were increased in the diabetic treated group compared to the diabetic control groups.&#x0D; Conclusion: Therefore, we conclude that AEHR can offer protection against diabetic-induced oxidative stress.

Pengaruh Pemberian Koro Benguk (Mucuna pruriens L) terhadap Kadar Gula Darah Tikus Diabetes Melitus yang Diinduksi Streptozotocin

Diabetes which is well-known in the community as diabetes in Indonesia is a chronic disease, which occurs when the pancreas does not produce enough insulin or when the body cannot utilize the insulin produced by its own products. The Provision of antioxidants in DM mice can reduce blood sugar levels. One of the herbs that can be used for control and management of blood sugar in diabetes is swollen koro. Koro Benguk (Mucuna pruriens L) is a plant that can be used as an alternative treatment because it contains antioxidants that can maintain health without causing toxic effects. The purpose of this study was to determine whether there is an effect of giving koro benguk coffee (Mucuna pruriens L) on blood sugar levels of Streptozotocin-induced Diabetes Mellitus Rats. This study is an experimental study of Sprague Dawley mice. A total of 35 male wistar rats were divided into 5 groups each: normal control (K1); diabetes control (K2); diabetic rats were given a large coffee extract 0.63 mg / g BW rat (P1); diabetic rats were given a large infusion of coffee koro 1.26 mg / g BW rats (P2). Diabetic rats were given an infusion of coffee koro benguk20,52 mg / g BW rats. Fasting blood glucose (GDP) levels were analyzed weekly for 3 weeks using the GOD-PAP method. The results of the study showed a decrease in blood sugar for 4 times the observation time in almost all treatment groups except the positive control group. The conclusions in this study were the provision of related coffee (Mucuna pruriens L) can reduce fasting blood glucose levels in Sprague Dawley rats with diabetes models significantly compared to controls.

Over-expression of arginine vasopressin in magnocellular neurosecretory cells of hypothalamus and its potential relationship with development of diabetic nephropathy

IntroductionWe aimed to assess our hypothesis that the expression changes of arginine vasopressin (AVP) in the magnocellular neurosecretory cells (MNCs) of hypothalamus and V2 receptor for AVP (RVP) in kidney may contribute to the pathogenesis of diabetic nephropathy (DN).Material and methodsTwenty-five male Wistar rats were randomly assigned to the control group and the diabetes mellitus (DM) group. Periodic acid-Schiff (PAS) staining and electron microscopy were used for morphological studies. Immunohistochemical staining for glial fibrillary acidic protein (GFAP) is standard for visualization of reactive astrocytes in the hypothalamus. Hypothalamus was used for immunofluorescence of AVP. Kidney was used for immunohistochemical staining of RVP. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used for quantitative determinations of AVP mRNA in hypothalamus and RVP mRNA in kidney. Western blot was used to measure the protein expression of AVP in hypothalamus and RVP in kidney.ResultsMorphological studies showed abnormalities in kidney and hypothalamus in the DM group. The number of neurons and the gray value of astrocytes in hypothalamus in the DM group were markedly decreased. The expression level of AVP in hypothalamus and the expression level of RVP in kidney of DM rats were significantly increased. The positive correlations between the proteinuria and expression (mRNA and protein) of AVP, proteinuria and expression (mRNA and protein) of RVP, and the expression of AVP and RVP levels were found.ConclusionsAVP was upregulated in the MNCs of hypothalamus and RVP was upregulated in kidney in streptozotocin-induced DM rats, indicating their potential roles in the development of DN.