Water quality is one of the topics of greatest concern worldwide, as it is a fundamental resource for life, whose constant monitoring is necessary. The objective of this work was to carry out seasonal monitoring of water quality in the Urutu-MS stream, occupied by more than 50% of eucalyptus silviculture forests. Water samples were collected from five points seasonally between autumn 2019 and summer 2020. Twenty-three parameters included such as temperature, pH, Dissolved oxygen, Conductivity, Alkalinity, Organic matter, Chloride, Sulfate, Total iron, Color, Turbidity, Total dissolved solids, Total suspended solids, Chemical Oxygen Demand, Total phosphorus, Hardness, Aluminum, Manganese, Magnesium, Sodium, Calcium, Copper and Dissolved Iron. The results showed that there is a difference in water quality between the seasons due to the different concentrations of physicochemical parameters. Spring was in class II, while the others were in class I, showing the influence of the onset of rain after the dry winter. Interactions between parameters followed conventional associations such as Alkalinity and Conductivity, SDT and Conductivity, Color and Turbidity, SST and Turbidity, Conductivity and Calcium, Conductivity and Magnesium. Alkalinity and TDS being the main parameters influenced by seasonality. Keywords: Paraná Watershed, Land use and Land Cover, Cerrado, Silviculture, Seasonality.