The layered nature of the parts produced by 3D printing makes them susceptible to freeze-thaw damage. This research investigates the effect of the freeze-thaw cycles on the tensile, bending, and fracture resistance of samples made of Polylactic acid (PLA) material. For this purpose, the samples with 100, 75, 50, and 25 infill percentages were subjected to 4, 8, and 12 freeze-thaw cycles. The results show that the infill percentage and cycle affect freeze-thaw resistance. So, although for 100% infill samples, the 4, 8, and 12 cycles averagely reduce the tensile strength by 5, 15, and 25. The same trends can also be seen for flexural strength and, more severely, fracture resistance. Reviewing the microstructure with a Scanning electron microscopy (SEM) device shows freeze-thaw's destructive effect (both the strand's surface and their joints). In the end, simple statistical analyses were presented to evaluate a model for anticipating the effect of freeze-thaw on mechanical resistance.