Strains Of Cellulolytic Bacteria Research Articles

Exploitation of cellulose degrading bacteria in bioconversion of agro-wastes

The issue of solid waste management has become worse as a result of rapid demographic growth and rising urbanisation. Therefore economical, efficient and quick yielding techniques are essential. In this study, composting of agro-wastes i.e., paddy straw, maize stover and vegetable waste with three strains of cellulolytic bacteria (CBC9, CBD4 and CBG2) was performed. The results showed that the C:N ratio of matured compost produced from bacterial inoculation ranged from 14–17:1, nitrification index (NI) ranged from 0.35 to 0.45, Cation exchange capacity (CEC) ranged from 76 to 88 cmol (P+) kg−1, whereas, total N, P and K content of composts increased by 75–127 %, 20–175% and 42–94%, respectively. The recovery of compost was highest in case of paddy straw compost (39%) produced from CBG2 inoculation which was followed by vegetable waste (38%) and maize stover compost (36%) produced from same bacterial inoculation. FT-IR, SEM and EDS studies suggested that final compost produced from inoculation of bacterial strain was more stabilized as compared to un-inoculated control, characterized by a decrease in aliphatic materials and an increase in aromatic groups, presumably related to bio-synthesis of humic substance.

Isolation, Screening and Identification of Cellulolytic Streptomyces corchorusii (Mn244066) From Soil Sample of Visakhapatnam

Fifty one isolates of cellulolytic bacteria were isolated from cow dung and two soil samples were obtained from Kambalakonda Wildlife Sanctuary and RCD Biodiversity Park in Visakhapatnam, Andhra Pradesh by Enrichment method in basal salt medium with cellulose as substrate for degradation. The cellulolytic activity of the isolated bacteria was determined by the diameter of the zone of hydrolysis by Gram’s iodine dye staining method. After primary screening, a total of fifty one isolates showed cellulolytic activity. Out of fifty one strains of cellulolytic bacteria, twenty three isolates from Kambalakonda Wildlife Sanctuary, seventeen isolates from RCD Biodiversity Park and Eleven isolates from cow dung sample obtained from cattle ranch, Visakhapatnam showed cellulase activity. Seven strains showed maximum hydrolytic value greater than 4.0 cm, nineteen strains showed average hydrolytic value between 3.0 and 3.9 cm and twenty one strains showed minimum hydrolytic value between 1.5 and2.9 cm. The potential isolates were obtained from Kambalakonda Wildlife Sanctuary and RCD Biodiversity Park than cow dung sample. The 13 C strain exhibited maximum hydrolytic value of 5.6 cm which was designated as KKV1. The strain KKVI was identified as Streptomyces corchorusii (MN244066) by morphological, cultural, biochemical and 16S rRNA sequence. The CMCase and FPase activity of the crude sample were examined by DNS method and found to be 0.21 U/ml and 0.041 U/ml respectively and the specific activity was 4.38 U/mg proteins and 0.86 U/mg proteins respectively. The present study emphasizes that the Streptomyces corchorusii have a higher cellulase activity and the soils of bio reserves have a lot of scope for isolating high cellulolytic bacteria which can be exploited for different industrial purposes.

The use of the enzymativeprobiotic additive "Rumit" in feeding lactating cows in the breeding factories of the Vologda region

Relevance and methodology. Milk production is effective for producers only in case of high productivity of the farmed livestock. In the prime cost of raw milk, feed costs account for over 60%, in connection with which the main task of modern science is the development of additives and preparations that can improve the use of feed by animals, which will ensure an increase in livestock productivity. The research team of OOO Biotrof has created an experimental preparation "Rumit" — a complex of live bacteria based on cultured strains of cellulolytic bacteria of deer rumen. The effectiveness of the enzymeprobiotic supplement was studied in the breeding farms of the SKHPK collective farm "Peredovoy" and JSC "Plemzavod Rodina" of the Vologda region on lactating cows with a productivity of over 8000 kg of milk per lactation. To conduct the experiment by the method of balanced groups for a complex of economically useful traits and genetic potential, a control and experimental group of 10–12 animals each were formed. Experienced groups were fed with the drug "Rumit" 50 g per head per day during the day.Results. For 90 days of the experiment, the total density of ciliates in the contents of the rumen of the experimental groups increased by 155.9–173.0 thousand ops / ml, and the significant difference with the control was 13.5.9–171.7 thousand ops / ml. The emergence of new genera of ciliates was noted: in the agricultural production complex the collective farm "Peredovoy" — Epidinium, in the JSC "Plemzavod Rodina" — Isotricha and Ophryoscolex. The increase in the average daily milk yield in the experimental groups was from 6.3 to 9.0% in the context of enterprises, an increase in the fat content in milk by 0.05–0.07% and protein by 0.03–0.07% was noted. As a result, in the SKHPK collective farm "Peredovoy" in the experimental group, 283 kg of milk of basic fat content were additionally obtained, and in JSC Plemzavod Rodina — 112 kg. In terms of per day from one head, it is possible to receive additional profit when feeding "Rumit" in the amount of 24.50 rubles. up to 120.15 rubles.

Biochemical Characterization of Cellulolitic Bacteria From Mangroves Weathered Wood In Muntok Sub district, West Bangka Regency

Acceleration of wood weathering in the mangrove area is possible with the role of cellulolytic bacteria. Cellulolytic bacteria producing cellulase enzymes are needed by the animal feed and agriculture industries. Mangroves in Muntok Subdistrict undergo a process of adaptation to environmental changes due to tin mining, including microorganisms. Exploration of new species and strains of cellulolytic bacteria in miningaffected mangroves is needed to enrich the collection and its potential use for human needs. This study aims to identify cellulolytic bacteria in mangrove weathered wood in Muntok District, West Bangka Regency through isolation, screening, and biochemical characterization. Three sampling locations, namely Sukal Mangrove, Peltim Mangrove, and Tembelok Mangrove result 22 bacterial isolates with 11 isolates showing the ability of cellulose degradation in qualitative tests using Lugol. The biochemical characterization of bacterial isolates with the greatest cellulose degradation was directed towards Citrobacter freundii and Vibrio alginolyticus in samples of Sukal Mangrove and Actinomyces bovis in isolates from Peltim Mangrove.

Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil

Lignocellulolytic enzyme production from submerged fermentation of paddy straw.

Five strains of cellulolytic bacteria and four strains of Phanerochaete chrysosporium were evaluated for the lignocellulolytic enzyme production during submerged fermentation (SmF) of paddy straw. Extra-cellular enzyme assay for CMCase, FPase, Cellobiase, Xylanase, Lignin peroxidase and Laccase enzymes was performed after 7 and 15 days of submerged fermentation. Cellulomonas cellulans MTCC 23, Cytophaga hutchinsonii NCIM 2338 and Phanerochaete chrysosporium MTCC 787 were found to produce higher lignocellulolytic enzyme activities than rest of the cultures after 15 days of fermentation.