To address the challenges posed by inadequate data and data utilization in multiple scenarios of fatigue loading, a Physics-informed Transfer Learning (PITL) model has been developed to predict the fatigue life of IN718 superalloy. Strain-controlled low-cycle fatigue tests were carried out at 400 °C with three distinct strain ratios, which were subsequently segmented for individual transfer learning tests. PITL models with significant engineering value were built by integrating transfer learning methodologies rooted in TrAdaBoost with a physics-based model that hinges on the principles of equivalent strain theory. The findings suggest that PITL models exhibit improved accuracy and greater robustness compared to both transfer learning and physics models.