There has been a disputation about how to achieve a pure mode I fracture and thus measure its toughness for an adhesively bonded bi-material double cantilever beam (DCB) specimen. This paper therefore develops a theoretical methodology to calculate the normal and shear stress distributions in the adhesive layer and to perform data reduction and mode partitioning for generic bi-material DCB specimens. The theoretical model is validated using experimental data. The adhesive layer in a generic bi-material DCB joint is loaded both in normal and shear based on the predicted stress distributions in the adhesive layer, resulting in a mixed mode fracture behavior. The prediction results substantiate that a strain-based design principle eliminates the shear stresses and thus leads to pure mode I fracture in bi-material DCB specimens. This paper justifies that the established data reduction method is applicable for measuring mode I fracture toughness when a bi-material DCB is designed according to the strain-based design criterion.