In the present study, the occurrence of microplastics (MPs) in the gut, gill, and muscle of edible fish Stolephorus indicus sampled from Tuticorin coastal regions of Tamilnadu, India was investigated. We recorded a total of 689 MPs which includes 510 and 179 MPs from males and females respectively. The total abundance of MPs was significantly (P < 0.05) higher in the gut followed by gills and muscle. The sex-wise distribution of average MPs showed high in the females' gut and compared to that in males. Further, the length wise distribution of MPs was higher in the muscle in both male and female fish, followed by other organs. The predominance of MPs in tissues were transparent and blue colour with fibers and fragments in both males and females. Besides, polyethylene terephthalate and nylon were evidenced by the Fourier-transform infrared spectroscopy spectrum in all organs of fishes.