Plant Stevia Rebaudiana Research Articles

Methyl jasmonate mitigates biochemical and phytochemical changes in salt stressed Stevia rebaudiana plants

This study explored the effects of salt stress on growth, oxidative stress, and steviol glycoside content in Stevia rebaudiana Bertoni plants cultivated in soil supplemented with methyl jasmonate (MeJA). Stevia plants were cultivated under normal and salt stress conditions, with and without MeJA supplements of 30 ?M, 60 ?M, and 120 ?M. Samples were harvested after the 1st, 3rd, 5th, 10th, and 15th day of treatment. The levels of chlorophyll (p<0.0001), carotenoids (p<0.0001), and antioxidant enzymes such as catalase (p<0.0001), superoxide dismutase (p<0.0001), APX (p<0.0001), and glutathione reductase (p<0.0001) were observed. The quantification of steviol glycosides, including stevioside (p<0.0001) and rebaudioside-A (p<0.0001), was studied by the most advanced hyphenated technique, LC-MS/MS. The study revealed that the oxidative stress responses were significantly improved in MeJA-treated plants compared to salt-stress control plants. The level of production of phenols (p<0.0001), flavonoids, total sugar, reducing sugar, and steviol glucosides was significantly altered in salt-stress plants. MeJA showed a dose- and time-dependent significant effect on the improvement of these factors over salt stress. In conclusion, MeJA not only improves the growth of plants but also reduces oxidative stress and enhances the level of phytochemicals under saline stress.

In vitro Molecular Mechanisms of Anticancer Activity of Stevioside in Human Osteosarcoma Cell Lines (Sarcoma Osteogenic).

Osteosarcoma (OS) is a rare and aggressive form of bone cancer that primarily affects the long bones of the body, such as the arms and legs. It is characterized by the uncontrolled growth of malignant cells in the bone tissue, leading to the formation of abnormal and painful bone masses. Steviol glycosides have been widely used as natural noncalorie sweeteners and are the collective name of the sweet substances found naturally in the plant Stevia rebaudiana, which is commonly called Stevia. Our study aimed to analyze the anticancer activity of Stevioside in OS. Stevioside was applied to OS cells, and the levels of Bcl xL, Bcl-2, and Bax were then estimated. The results of three separate studies, each carried out in triplicate, were expressed as the mean ± standard errors of the mean (SEM). One-way ANOVA was used for statistical analysis. The findings showed that the effect of Stevioside on sarcoma osteogenic cells with mean ± SEM as 0.74 ± 0.05, 0.69 ± 0.09, 0.46 ± 0.09 for Bcl-xL gene, 0.98 ± 0.06, 0.58 ± 0.07, 0.5 ± 0.07 for Bcl-2 gene, and 1.2 ± 0.08, 1.45 ± 0.11, 1.67 ± 0.12 for Bax gene, respectively, when treated with untreated control cells. The study concludes its action against bone OS cells was significant with apoptotic induction. Stevia has a wide range of health benefits as well as being a plant-based diet it has less of side effects and promoting features even by intaking it daily along with other medicines.

Ent-kaurane glycosides from Stevia rebaudiana and their α-glucosidase inhibition

Phytochemical investigation of the water soluble fraction of the plant Stevia rebaudiana led to isolation of 10 ent-kaurane glycosides, including two new derivatives namely stevibiosides A and B (1 and 2). Their structures were elucidated by extensive analysis of their 1D, 2D NMR, and HRQTOF mass spectra as well as quantum chemical calculations. Conformation studies and DFT-calculated chemical shifts combined with simple (R2, MAE) and DP4+ statistical analyses allow for the complete characterization of compounds 1 and 2. Among isolates, stevibioside A (1) exhibited potent α-glycosidase inhibitory effects with IC50 value of 8.9 ± 0.45 μM. Inhibitory kinetics analysis indicated that 1 had effect with an uncompetitive inhibition type and the Ki value of 2.18 ± 0.81 μM. In addition, docking studies were then used to analysis of the binding modes of the compounds 1 and 2 over the crystallographic structure of yeast α-glucosidase (pdb 3A4A) active site in comparison with acarbose as a standard inhibitor of this enzyme.

Identifying Potential Sources of Phthalate Contamination in the Leaves of Stevia Rebaudiana (Bertoni) and the Development of Removal Technology.

Steviosides extracted from the leaves of the plant Stevia rebaudiana are increasingly used in the food industry as natural low-calorie sweeteners. Phthalates in food are often assumed to arise from food containers or packaging materials. Here, experiments were carried out to identify the potential sources of DMP, DBP, DIBP, and DEHP in the leaves of stevioside through investigation of their content in native stevioside tissues, soils, and associated agronomic materials. The results show that phthalate contamination was present in all the samples tested, and the influence of regional factors at the provincial level on the content of plasticizers in stevia leaves was not significant. Phthalates in stevia leaves can be absorbed into the plant body through leaves and roots. Using resin removal, the phthalate content in stevioside glycosides was reduced to less than 0.05 ppm, and some indicators were far lower than the limit standard in EU food.

Exploring Stevia: A Natural Sweetener with Multifaceted Health Benefits

Natural sweeteners, especially those derived from plants, have become popular as low-calorie options compared to traditional sweeteners. Such discussion explores the world of natural sweeteners, emphasizing the growing demand for alternatives to artificial ones like saccharin and cyclamates. These sweeteners, sourced from plants, fruits, and honey, provide a healthier choice with fewer calories. Stevia, extracted from the Stevia rebaudiana plant, is a notable natural sweetener due to its zero-calorie content and strong sweetness. The review explores the background of Stevia, its glycoside compounds, and how it attains its sweetness. The pharmacological aspects of Stevia are also explored, highlighting its potential benefits in managing Type 2 diabetes, reducing blood pressure, alleviating inflammation, acting as an antioxidant, and showing anticancer properties. Studies suggested that Stevia’s active ingredients, are primarily Stevioside and Rebaudioside A, contribute to its sweetness. The chemical composition of these active ingredients is influenced by factors like geographical location, cultivars, and processing methods. Stevia offers advantages such as low caloric content, suitability for diabetics, and benefits for dental health, making it an appealing sugar substitute for those focused on weight management and a health-conscious lifestyle. However, it is important to note potential drawbacks, including a bitter aftertaste, individual sensitivities, and regulatory considerations. Some commercial Stevia products may contain additives that impact their nutritional profile. Despite these factors, high-purity Stevia extracts have received safety approvals from global food safety authorities. In summary, Stevia emerges as a natural sweetener with the potential to address health-related concerns linked to traditional sugar consumption.

Navigating Weight Management with Stevia: Insights into Glycemic Control

Stevia is a natural sweetener that has gained attention as a potential sugar substitute for glycemic control and weight management. Extensive research has shown that high sugar consumption is linked to obesity, dental caries, and other health issues. Stevia, derived from the Stevia rebaudiana plant, offers sweetness without calories and has a favorable glycemic profile. Studies have demonstrated its effectiveness in regulating blood glucose levels and reducing overall sugar and caloric intake. Consumer attitudes suggest a positive reception of Stevia as a sugar substitute among health-conscious individuals. However, further research is needed to understand its long-term effects and consumer sentiments. Future investigations should prioritize human clinical trials, targeted studies in diabetic populations, and exploration of Stevia’s interaction with gut microbiota, among other aspects. Stevia shows promise as a health-conscious alternative in glycemic control and weight management.

Molecular Approach to Identify Anti-inflammatory Potential of Stevioside in HFD-induced Type 2 Diabetic Rats: Evidence From in Vivo Study

Stevioside is a natural sweetener derived from the leaves of the Stevia rebaudiana plant. It has gained popularity as a sugar substitute due to its intense sweetness without adding calories or affecting blood sugar levels, making it a suitable option for people with diabetes or those looking to reduce their sugar intake. Studies have shown that stevioside has glucose lowering effects. Previous studies have shown that it has significant role in skeletal muscle but its role on expression of inflammatory signaling molecules in adipose tissue against high diet and sucrose-induced type-2 diabetes in experimental rats is yet to be done. The current research was undertaken to investigate if stevioside could also exert its antidiabetic effects by circumventing adipocyte induced inflammation, a key driving factor for insulin resistance in obese individuals. Effective dose of stevioside (20 mg/kg b.wt) was administered orally for 45 days to high fat diet and sucrose induced type-2 diabetic rats. Interestingly, stevioside treatment restores the elevated serum levels of proinflammatory cytokines including tumor necrosis factor-α (TNF-α) and sterol regulatory element binding protein-1c (SREBP-1c) and enhances Peroxisome Proliferator–activated receptor-γ (PPAR-γ) in adipocytes of diabetic rats. The gene expression of IR, GLUT4 and PPAR-γ mRNA were also significantly activated in stevioside treated groups but reduced IL-1 beta, IlL-6, IKKB, TNF-alpha and NFkB mRNA expression in diabetic adipose tissue. More importantly, stevioside acts very effectively as metformin to circumvent inflammation and insulin resistance in diabetic rats. Our results clearly show that stevioside inhibits obesity induced insulin resistance by ameliorating the inflammatory events and upregulating insulin signalling molecules. Keywords: Stevioside, HFD-T2DM, pro inflammation, insulin signalling; adipose tissue, obesity; signaling pathways; Therapautics.

IN VITRO EVALUATION OF ANTIOXIDANT AND CYTOTOXIC ACTIVITIES ON HUMAN CERVICAL CANCER CELLS IN THREE DIFFERENT PLANT EXTRACTS FROM TURKEY

The objective of this investigation was to assess the total phenolic and flavonoid content within acetone and water extracts derived from Erica manipuliflora, Ferula communis, and Stevia rebaudiana plants. Additionally, the in vitro antioxidant and cytotoxic effects of these extracts were evaluated using the human cervical cancer (HeLa) cell line as a model. Among the three plant species examined, the highest concentrations of total phenols and flavonoids were found in the acetone extract of E. manipuliflora, measuring 365.29 mg GAE/g DW and 105.42 mg QE/g DW, respectively. Acetone and water extracts of E. manipuliflora showed higher DPPH scavenging activities (IC50: 37.57 and 33.27 µg/mL, respectively) compared to other plants. furthermore, E. manipuliflora acetone extract (IC50: 97.35 µg/mL) exhibited the highest inhibition in the HeLa cell line treated with the extracts, thus being the most effective extract on this cell line. As a result of the analyzes, it has been shown that F. communis and S. rebaudiana and especially E. manipuliflora are important natural antioxidant sources with their strong radical scavenging effects, as well as the anticancer potential of these plants.

Potential Effects of Steviol Glycosides from Stevia Rebaudiana Leaf on Marine Life: A Mini Review

Steviol glycosides, extracted from the leaves of the Stevia rebaudiana plant, have gained widespread acceptance as a non-caloric sweetening alternative used in the food industry. Despite their growing popularity, the potential consequences of these sweeteners on marine ecosystems have not been thoroughly examined. This review aims to outline current knowledge and identify areas for future research.

A New Healer in the Treatment of Diabetes and Diabetic Wounds: Stevia rebaudiana

Diabetes, one of the most common diseases in the world, causes a decrease in the formation of macrophages, endothelial cells, keratinocytes, platelets, and, fibroblasts, which play a role in the cellular response to tissue damage in wound healing, and a decrease in the cytokines they secrete, inflammatory mediators, or growth factors, making wound healing difficult. As the healing period of the wounds increases, the psychology of the patients is adversely affected by the inability to return to their daily routines, the loss the of workforce increases, and the country's economy is adversely affected. In the last decades, because of the increase in many diseases such as diabetes and diabetes-related wounds, the use of calorie-free foods and herbal treatment methods has been preferred. Plants are increasingly being used as a source of pharmaceutical medications since they are simple to obtain, have no negative side effects, are affordable, and are simple to utilize. In order to meet these needs, sweeteners containing stevia extracts or components, obtained from the Stevia rebaudiana plant, that are calorie-free and 300 fold sweeter than cane sugar, have been on the market for some time. Despite the fact that Stevia rebaudiana, often known as sugar grass, is not a commonly utilized plant around the globe, it is possible to find literature that claims it is used as a preventive and curative for a variety of diseases. Although Stevia rebaudiana has various biological effects such as antioxidant, antidiabetic, antitumor, and antimicrobial, knowledge of diabetes and its effects on diabetic wound healing are limited. The purpose of this review is to evaluate how effectively Stevia rebaudiana treats diabetes and diabetic wounds.

Effects of polystyrene nanoplastics exposure on in vitro-grown Stevia rebaudiana plants

Nanoplastics (NPs) as environmental contaminants have received increased attention in recent years. Numerous studies have suggested possible negative effects of plants exposure to NPs, but more data are needed with various plants under different exposure conditions to clarify the underlying phytotoxicity mechanisms. In this study, we investigated the effect of polystyrene nanoplastics (PSNPs; 28.65 nm average diameter) exposure (10, 100 and 250 mg/L) on plant morphology and production of relevant metabolites (steviol glycosides, chlorophylls, carotenoids, and vitamins) of in vitro-grown Stevia rebaudiana plantlets. Additionally, we used dark field microscopy combined with fluorescence hyperspectral imaging for the visualization of internalized PSNPs inside plant tissues. At higher concentrations (>100 mg/L), PSNPs were shown to aggregate in roots and to be transported to leaves, having a significantly negative impact on plant growth (reduced size and biomass), while increasing the production of metabolites compared to controls, most probably because of response to stress. The production of steviol glycosides presented a biphasic dose-response suggestive of hormesis, with the highest values at 10 mg/L PSNPs (1.5–2.2-fold increase compared to controls), followed by a decline in production at higher concentrations (100 and 250 mg/L), but with values comparable to controls. These results are promising for future in vivo studies evaluating the effect of NP exposure on the production of steviol glycosides, the natural sweeteners from stevia.

The Noncaloric Sweetener Rebaudioside A Stimulates Glucagon-Like Peptide 1 Release and Increases Enteroendocrine Cell Numbers in 2-Dimensional Mouse Organoids Derived from Different Locations of the Intestine

Background: Glucagon-like peptide 1 (GLP-1) contributes to satiety and plays a pivotal role in insulin secretion and glucose homeostasis. Similar to GLP-1, peptide YY (PYY) and cholecystokinin also influence food intake. The secretion of these hormones by enteroendocrine cells along the intestine is modulated by nutrients. Preparations from the Stevia rebaudiana plant, including rebaudioside A, are increasingly being used as noncaloric sweeteners.Objective: We investigated the effects of rebaudioside A on enteroendocrine cells by assessing both cell numbers as well as their secretory capacity in an organoid model.Methods: A 2-dimensional organoid model derived from duodenal, jejunal, and ileal crypts of a C57BL/6J mouse was developed and characterized with the use of gene expression and immunofluorescence. We stimulated these organoids with 10 mmol/L rebaudioside A for 1 h and measured their GLP-1, PYY, and cholecystokinin release. We also analyzed the effects of rebaudioside A on gene expression in enteroendocrine cells after an 18-h incubation.Results: The 2-dimensional organoids contained crypt cells and differentiated villus cells, including enterocytes and goblet and enteroendocrine cells. These enteroendocrine cells stained positive for GLP-1, PYY, and serotonin. The cultured 2-dimensional organoids maintained their location-specific gene expression patterns. Compared with the control, rebaudioside A induced GLP-1 secretion 1.7-fold in the duodenum (P < 0.01), 2.2-fold in the jejunum (P < 0.01), and 4.3-fold in the ileum (P < 0.001). PYY release was increased by rebaudioside A 3-fold in the ileum compared with the control (P < 0.05). Long-term (18-h) stimulation with the sweetener induced the expression of the enteroendocrine-specific markers chromogranin A, glucagon, Pyy, and cholecystokinin 3.5- (P < 0.001), 3.5- (P < 0.001), 3.8- (P < 0.05), and 6.5-fold (P < 0.001), respectively.Conclusions: These results show novel ex vivo effects of rebaudioside A on enteroendocrine cells of the mouse small intestine and highlight potentially new applications for rebaudioside A in metabolic diseases.

Transglycosylation of Stevioside by a Commercial β-Glucanase with Fungal Extracted β-Glucans as Donors

Alternative sweeteners, such as steviol glucosides from the plant Stevia rebaudiana Bertoni, are becoming increasingly popular for the design of next-generation foodstuffs. However, the bitter aftertaste of native steviol glucosides is one of the main reasons behind consumer reluctance towards stevia-containing products. Biocatalysis could be a sustainable solution to this problem, through addition of glucosyl moieties to the molecule. Glycoside hydrolases are enzymes performing transglycosylation reactions, and they can be exploited for such modifications. In the present work, the commercial β-glucanase Finizym 250L® was employed for the transglycosylation of stevioside. After optimization of several reaction parameters, the maximal reaction yield obtained was 19%, with barley β-glucan as the glycosyl donor. With the aim to develop a sustainable process, β-glucan extracts from different fungal sources were prepared. Pulsed Electric Field pretreatment of mycelial biomass resulted in extracts with higher β-glucan content. The extracts were tested as alternative glucosyl donors, reaching up to 15.5% conversion yield, from Pleurotus-extracted β-glucan. Overall, in the present work a novel enzymatic process for the modification of stevioside is proposed, with concomitant valorization of β-glucans extracted from fungal biomass, potentially generated as a byproduct from other applications, in concert with the principles of circular economy.Graphical

Biosynthesis of silver nanoparticles using extracts of Stevia rebaudiana and evaluation of antibacterial activity.

The present study reveals a simple, non-toxic and eco-friendly method for the "green" synthesis of Ag-NPs using hydroponic and soil medicinal plant Stevia rebaudiana extracts, the characterization of biosynthesized nanoparticles, as well as the evaluation of their antibacterial activity. Transmission electronic microscopy (TEM) and Dynamic Light Scattering (DLS) analysis confirmed that biosynthesized Ag-NPs are in the nano-size range (50-100nm) and have irregular morphology. Biogenic NPs demonstrate antibacterial activity against Escherichia coliBW 25,113, Enterococcus hirae ATCC 9790, and Staphylococcus aureus MDC 5233. The results showed a more pronounced antibacterial effect on E. coli growth rate, in comparison with Gram-positive bacteria, which is linked to the differences in the structure of bacterial cell wall. Moreover, the Ag-NPs not only suppressed the growth of bacteria but also changed the energy-dependent H+-fluxes across the bacterial membrane. The change of H+-fluxes in presence of H+-translocating systems inhibitor, N,N'-dicyclohexylcarbodiimide (DCCD), proves the effect of Ag-NPs on the structure and permeability of the bacterial membrane. Overall, our findings indicate that the Ag-NPs synthesized by medicinal plant Stevia extracts may be an excellent candidate as an alternative to antibiotics against the tested bacteria.

Inisiasi, pertumbuhan, dan perkembangan kalus embriogenik tanaman stevia (Stevia rebaudiana)

The leaves of sweetener plant Stevia rebaudiana contain secondary metabolites of steviol glycosides which are very sweet, with no calorie and zero glycemic index. Propagation of stevia by seeds is ineffective due to its low germination rate and diverse progenies. The tissue culture of stevia can be used to mass propagate rapidly and is commonly conducted by shoot multiplication. Up to now, the technology of somatic embryogenesis (SE) in stevia has not been successful yet. SE is developed to increase the production scale, rejuvenate clonal-propagated plants, and plant genetic transformation. The research objective was to develop protocols for the initiation, proliferation, and development of embryogenic calli of stevia as potential materials for SE. The explants used were young leaves, nodes, and internodes of axenic plantlets of stevia BX clone. The explants were cultured on MS solid media containing different concentrations of auxin and cytokinin for callus initiation. Callus emerged after 2-3 weeks of culture. The calli obtained were proliferated by subculturing several times as material stocks for indirect SE. MS solid media added with 1 µM 3,4-D and 16 mM CaCl2 gave the highest callus multiplication rate (4.7 times in 3 weeks). The selection of embryogenic calli was made continuously to obtain a pure line of embryogenic calli. Three types of calli attained were friable, fast-growing, yellowish calli, shiny nodular calli, and greenish nodular calli. Histological studies revealed that cells of the nodular calli had been differentiated to potentially formed somatic embryos.

Study The Anti –Inflammatory Effect Of Stevia Rebaudiana Sweetnessin Vitro

Because of the high rates of diabetes, overweight disorders and fatness, the need and interest in finding alternatives to sugar has increased, especially in the industrial sector in parallel with reducing the daily intake of sugar by human. The idea of this research was developed to identify the active ingredients which include in the plant aqueous extract of Stevia rebaudiana. Chemical examination showed the glycosides and phenolic compounds presence of in the Stevia rebaudiana plant. The effect of Stevia rebaudiana leaves products aqueous extract as anti-inflammation measured in vitro with two procedures , The repression of protein denaturation and hemolysis stimulated by heating and results showed Stevia rebaudiana efficiency with compared to typical medicine (Aspirin) and negative control groups.

Stevioside Potentiates Calcium Activity and Insulin Secretion in Human Pancreatic Islets Through Potentiation of TRPM5

TRPM5 is a calcium-activated monovalent cation channel that is expressed in human and murine islets. Ion channels of the TRP family are multimodal sensors and are activated by different stimuli such as voltage, temperature and ligands, including natural compounds. Previous work showed that mice lacking TRPM5 have increased postprandial blood glucose levels and an impaired insulin secretion. There are increased observations of TRPM5 mutations in patients suffering from diabetes or metabolic syndrome. Stevioside or stevia is a sweet-tasting organic compound isolated from the plant Stevia rebaudiana, and is widely used as a low-caloric sweetener. Despite the use of stevioside in consumer products, there is limited knowledge about its molecular interaction in the body. It was previously shown in mice that stevioside potentiates TRPM5, which leads to increased insulin secretion and ultimately better glycemic control in diabetic animal models. In this work, we translate these observations to freshly isolated human islets. We show that TRPM5 expression is limited to a subset of the insulin-expressing beta-cells in islets from non-diabetic donors. In islets isolated from diabetic donors, we also see distinct TRPM5 positive cells that are insulin negative. There are substantial differences observed in the microanatomy of the islets and the functional synchronization of calcium signaling in beta cells between mouse and man. In perifusion studies, there is a potentiation of the glucose-induced insulin secretion with the application of stevioside. These studies provide insight in the molecular mechanism of the action of stevioside in the human islets. Furthermore, we confirm the importance of TRPM5 in modulating the glucose-stimulated insulin secretion in human islets. Modulating TRPM5 with stevioside constitutes a potential novel

Allometric models for non-destructive leaf area measurement of stevia: an in depth and complete analysis

ABSTRACT Leaf area measurement is pivotal for plant physiologists. Hence, accurate measurement of their leaf area is incredibly relevant in agronomic terms. The plant Stevia rebaudiana is a sucrose-free plant species that is now vital to the global production of sucrose-free foods. Here, we estimated S. rebaudiana leaf area using a nondestructive methodology comprising allometric equations. Through leaf length (L), leaf width (W), and/or their product (LW) the leaf area was determined. One thousand leaves were sampled from four distinct S. rebaudiana genotypes for model construction. Linear or power models were generated, and the best equation was selected using a statistical criterion. The statistical criteria indicated that the linear models best suited all genotypes tested, included a function of LW, exhibited increased stability, and precisely estimated coefficients. ANOVA revealed that both generalized and combined equations were feasible. Nevertheless, grouping all genotypes into a single model was not possible as the genotype leaf architectures were very dissimilar.

Lack of potential carcinogenicity for steviol glycosides - Systematic evaluation and integration of mechanistic data into the totality of evidence

Steviol glycosides are present in the leaves of the Stevia rebaudiana plant, have a sweet taste, and have been used as a sweetener for centuries. To build on previous authoritative safety assessments of steviol glycosides, a systematic assessment of mechanistic data related to key characteristics of carcinogens (KCCs) was conducted. Over 900 KCC-relevant endpoints from peer-reviewed literature and high-throughput screening data (ToxCast/Tox21) were identified across individual steviol glycosides and derivatives, metabolites, and whole leaf extracts. Most data (both in vivo and in vitro, including human cells), showed inactivity. Studies were weighted according to quality and relevance. Although data were available for eight of the ten KCC, genotoxicity, oxidative stress, inflammation, and cell proliferation/cell death represent the KCCs with the most data. The data for these KCC primarily show beneficial activity (anti-inflammatory, antioxidant, and anti-proliferative). Following integration across all data, and accounting for study quality and relevance, the totality of the evidence demonstrated an overall lack of genotoxic and carcinogenic activity for steviol glycosides. This is in agreement with previous regulatory decisions, and is consistent with the lack of tumor response in two-year rodent cancer bioassays. The findings support prior conclusions that steviol glycosides are unlikely to be carcinogenic in humans.

Phytochemical and Biological Investigation of Stevia rebaudiana (Bert.) Leaves Grown in Bangladesh

Three fractions obtained by Kupchan partitioning of 70% methanol extract of leaves of Stevia rebaudiana with n-hexane, dichloromethane and ethyl acetate designated by HSR, DSR and ESR were investigated for their antioxidant and antimicrobial activities. HSR and ESR exhibited prominent free radical scavenging activity having IC50 value of 11.59 and 10.38 μg/ml compared to standard, ascorbic acid (IC50 value 6.05 μg/ml). HSR and ESR also demonstrated significant antibacterial activity by disc diffusion method on some Gram-positive and Gramnegative bacteria. Among the fractions ESR showed the highest antioxidant and antibacterial activity. No significant antifungal activity was observed for any fraction. Preliminary phytochemical screening of all fractions showed the presence of alkaloids, saponin, flavonoids, tannin, carbohydrate and triterpenoids. Total three compounds have been isolated from ESR fraction among which two known compounds (1-2) were characterized as quercitrin (1) and physcion (2) through 1D NMR spectroscopic technique and other one could be a 9,10- anthraquinone derivative which was not characterized yet. The compound physcion (2) is first time report from the plant Stevia rebaudiana.&#x0D; Dhaka Univ. J. Pharm. Sci. 19(2): 191-197, 2020 (December)