Background and Study Aim. In the context of increasing stress and declining health among female college students, there is an urgent need for effective methods to enhance their physical and mental well-being. The aim of this study is to analyze the impact of Baduanjin exercise on the physical fitness and mental health of female college students. Materials and methods. Sixty female college students at University were randomly selected from 150 volunteers to participate in this study. They were equally divided into an experimental group and a control group, with 30 students in each. All 60 participants completed the experiment. The study's protocol was conducted in accordance with ethical standards and was approved by the Institutional Review Board of University. Informed consent was obtained from all individual participants involved in the study. The experimental group received a 16-week intervention of traditional Baduanjin exercise, while the control group engaged in other unfixed sports activities synchronously. Results. After the experiment, the average weight of the experimental group decreased significantly from 52.41±6.35 kg to 50.06±5.46 kg (P<0.01). Body mass index, waist circumference, and other indicators also showed significant improvements. The step test index in the experimental group increased from 45.09±4.45 to 50.72±4.46, which was significantly different from the baseline (P<0.01). Improvements were noted in vital capacity, grip strength, and sit-up performance, all showing significant differences from baseline measures (P<0.01). The standing long jump and 800 m running performances in the experimental group showed significant improvement compared to pre-experiment measurements (P<0.05). Additionally, all mental health indicators in the experimental group demonstrated a downward trend, with significant differences in 10 indicators such as somatization, obsessive-compulsive symptoms, interpersonal sensitivity, and depression (P<0.05 or P<0.01). Moreover, there was a significant inter-group difference in motor skills and physical fitness between the experimental and control groups in the 800 m run (230.78±30.61 vs. 231.32±32.15) and standing long jump (1.81±0.33 vs. 1.78±0.42) after the intervention (P<0.05). Furthermore, significant differences were observed in mental excitement and participation in the experimental group before and after the Baduanjin exercise as assessed by Baduanjin’s Self-Perception Inventory (P<0.01). Conclusions. This study demonstrates that a 16-week program of traditional Baduanjin exercise significantly improves both physical and mental health parameters among female college students. These findings suggest that Baduanjin exercise can be an effective intervention for enhancing physical fitness and alleviating psychological distress in this population. The results underscore the potential of integrating traditional physical activities into health promotion strategies for young adults in educational settings.