Application Of Static Magnetic Field Research Articles

Low-intensity magnetization pretreatment to enhance biomethane generation and the abundance of key microorganisms for anaerobic digestion of sewage sludge

This work explores the impact of static magnetic field (SMF) intensity on biomethane production from anaerobic digestion (AD) of sewage sludge. Two different SMF intensities (20 mT and 1.5 T) were applied to magnetize the sludge destined to the AD process. The magnetic pretreatment at 20 mT was particularly effective, as it increased biomethane production by 12.7 % compared to the control test. On the contrary, exposing the sludge to 1.5 T adversely affected biomethane production, resulting in a 15.1 % decrease. The positive correlation observed between low-intensity SMF exposure and enhanced biomethane yield, in contrast to the inhibitory effect of high-intensity SMF, suggests the existence of an optimal intensity threshold within the lower range for maximizing methane production. The impact of magnetic pretreatment on the anaerobic microbial community was investigated through high-throughput sequencing analysis of magnetized sludge samples. This approach enabled the identification of specific shifts in microbial populations associated with SMF exposure, thereby elucidating the role of SMF in modulating key microbial communities for the AD process. The findings of this study provide insights into the potential mechanisms underlying these responses and underscore the potential of SMF application for improving the anaerobic valorization of sewage sludge.

Static magnetic fields in regenerative medicine.

All organisms on Earth live in the weak but ubiquitous geomagnetic field. Human beings are also exposed to magnetic fields generated by multiple sources, ranging from permanent magnets to magnetic resonance imaging (MRI) in hospitals. It has been shown that different magnetic fields can generate various effects on different tissues and cells. Among them, stem cells appear to be one of the most sensitive cell types to magnetic fields, which are the fundamental units of regenerative therapies. In this review, we focus on the bioeffects of static magnetic fields (SMFs), which are related to regenerative medicine. Most reports in the literature focus on the influence of SMF on bone regeneration, wound healing, and stem cell production. Multiple aspects of the cellular events, including gene expression, cell signaling pathways, reactive oxygen species, inflammation, and cytoskeleton, have been shown to be affected by SMFs. Although no consensus yet, current evidence indicates that moderate and high SMFs could serve as a promising physical tool to promote bone regeneration, wound healing, neural differentiation, and dental regeneration. All in vivo studies of SMFs on bone regeneration and wound healing have shown beneficial effects, which unravel the great potential of SMFs in these aspects. More mechanistic studies, magnetic field parameter optimization, and clinical investigations on human bodies will be imperative for the successful clinical applications of SMFs in regenerative medicine.

Static Magnetic Field Stimulation Enhances Shunting Inhibition via a SLC26 Family Cl- Channel, Inducing Intrinsic Plasticity.

Magnetic fields are being used for detailed anatomical and functional examination of the human brain. In addition, evidence for their efficacy in treatment of brain dysfunctions is accumulating. Transcranial static magnetic field stimulation (tSMS) is a recently developed technique for noninvasively modifying brain functions. In tSMS, a strong and small magnet when placed over the skull can temporarily suppress brain functions. Its modulatory effects persist beyond the time of stimulation. However, the neurophysiological mechanisms underlying tSMS-induced plasticity remain unclear. Here, using acute motor cortical slice preparation obtained from male C57BL/6N mice, we show that tSMS alters the intrinsic electrical properties of neurons by altering the activity of chloride (Cl-) channels in neurons. Exposure of mouse pyramidal neurons to a static magnetic field (SMF) at a strength similar to human tSMS temporarily decreased their excitability and induced transient neuronal swelling. The effects of SMF were blocked by DIDS and GlyH-101, but not by NPPB, consistent with the pharmacological profile of SLC26A11, a transporter protein with Cl- channel activity. Whole-cell voltage-clamp recordings of the GlyH-101-sensitive Cl- current component showed significant enhancement of the component at both subthreshold and depolarized membrane potentials after SMF application, resulting in shunting inhibition and reduced repetitive action potential (AP) firing at the respective potentials. Thus, this study provides the first neurophysiological evidence for the inhibitory effect of tSMS on neuronal activity and advances our mechanistic understanding of noninvasive human neuromodulation.

MEDICINAL BIOMAGNETISM FOR ANALGESIA - APPLICATION OF STATIC MAGNETIC FIELD THROUGH MODERN TRAUMA PROTOCOL

Pain is one of the most common reasons people seek medical care and is related to most disease states. It is estimated that the prevalence of pain varies from 37% to 70%, generating functional limitations and negative impacts on quality of life. Medicinal Biomagnetism (MB) is a therapeutic approach that corrects bioelectromagnetic dysfunctions through the application of Static Magnetic Fields (SMF), acting on the most varied pathological states. SMF can be applied in a controlled manner, locally to specific parts of the human body, aiming to improve inflammatory, infectious and dysfunctional conditions that can lead to pain. Objective: To evaluate the analgesic effects of applying one of the MB protocols, the Modern Trauma Pair (MTP), in participants with pain. Methodology: A cross-sectional observational study was carried out, where the MTP was applied to 30 participants who had some type of pain, using the Visual Analogue Scale, to assess the intensity of subjective pain. Results: A statistically significant difference (p<0.001) was observed between the times before applying the protocol (average of 6.87), and 15 minutes after applying the MTP. There was a progressive decline in reported pain perception, reaching an average of 4.74 for acute pain and 4.84 for chronic pain, after 60 minutes of use. Conclusion: MTP can be an important therapeutic approach for pain relief. The Medicinal Biomagnetism protocol, MTP can contribute quickly and with minimal side effects to analgesia in different types of pain.

Piggery wastewater treatment with a Chlorella microalgae-bacteria consortium and static magnetic field

Wastewater discharges into the environment without adequate treatment is a problem still unresolved. That is why alternative technologies, such as applying static magnetic field (SMF), are currently being sought. The objective of this research was to evaluate the effect of a 47.23 mT SMF on the removal of nitrogen (N), phosphorus (P), and the growth of a Chlorella microalgae-bacteria consortium grown in piggery wastewater under different photoperiods. SMF application increased culture productivity, with maximum values of 0.030 g/ Ld−1 in cultures exposed to SMF in a 16:8 h light/dark photoperiod and 0.038 g/ Ld−1 for the 24:0 h light/dark photoperiod. Furthermore, SMF application increased the microorganisms' photosynthetic efficiency (QY), regardless of the photoperiod used. No significant differences were observed on day 21 regarding nutrient removal between the treated and control cultures. Removal percentages of 93.41 % N and 95.56 % P were obtained for the cultures exposed to SMF in the 16:8 h light/dark photoperiod, and 94.78 % N and 95.59 % P for cultures exposed to SMF during the 24:0 h light/dark photoperiod. In general, SMF acts mainly on the growth of microorganisms.

Handwritten digit recognition by spin waves in a Skyrmion reservoir

By performing numerical simulations for the handwritten digit recognition task, we demonstrate that a magnetic skyrmion lattice confined in a thin-plate magnet possesses high capability of reservoir computing. We obtain a high recognition rate of more than 88%, higher by about 10% than a baseline taken as the echo state network model. We find that this excellent performance arises from enhanced nonlinearity in the transformation which maps the input data onto an information space with higher dimensions, carried by interferences of spin waves in the skyrmion lattice. Because the skyrmions require only application of static magnetic field instead of nanofabrication for their creation in contrast to other spintronics reservoirs, our result consolidates the high potential of skyrmions for application to reservoir computing devices.

A Novel Magnetic Manipulation Promotes Directional Growth of Periodontal Ligament Stem Cells.

Periodontium is the rally of soft and hard tissues, which will be devastated continuously by the compromise of periodontitis. Current periodontal therapeutic methods cannot effectively reconstruct periodontal ligament (PDL), which is oriented at an angle with tooth root and combined hard tissues to form cementum-PDL-alveolar bone complex. Hence, it is urgent to find new techniques for PDL reconstruction to achieve functional regeneration of periodontium. Herein, we developed a novel method to manipulate the distribution and growth of periodontal ligament stem cells (PDLSCs) by utilizing highly paralleled static magnetic field (SMF) and magnetic nanoparticles (MNPs). PDLSCs were incubated with MNPs in vitro to label with them. Meanwhile, CCK8 and live/dead cell staining assay were used to detect the impact of SMF and MNPs on cell viability. The directional migration and growth of PDLSCs were visualized under microscope. Furthermore, real-time quantitative PCR and western blot were utilized to calculate the expression level of PDL-related genes. The results showed that PDLSCs could rapidly take up MNPs without compromising cell proliferation and viability, consequently endowed with the ability to respond via magnetic force. The cell migration analysis indicated that PDLSCs could move along the magnetic induction line, testifying that SMF exerted forces on PDLSCs that labeled with MNPs. It was demonstrated that collective application of SMF and MNPs not only induced PDLSCs organized and grew directionally, but also initiated elongation of cells and nucleus. Furthermore, the morphological alteration of the nucleus could also effectively enhance the gene and protein expression of Collagen Ⅰα2, Collagen Ⅲ, and Periostin, suggesting the capability of PDLSCs to differentiate into PDL. In conclusion, this study exhibits a new approach for directional reconstruction of PDL to obtain physiological and functional regeneration of periodontium. The Clinical Trial Registration number: WCHSIRB-D-2022-458.

Application of Static Magnetic Field to Modify Heat and Mass Transfer during Welding of Shipbuilding Steel

Welding process is very important in numerous industries ranging from automotive and aviation to shipbuilding and pressure vessel production. Nowadays new methods to improve the productivity and quality of various welding techniques are searched. In many industrial applications even small process optimizations may lead to significant cost and energy savings and new applications. Large plate welding is particularly important in shipbuilding industry. It is common to weld multiple times to join thick plates, but this approach is not optimal from energy and time effectiveness and outcome quality is limited. Alternative is single high heat input welding, which causes various problems related to rapid local overheating and the formation of inhomogeneous post-weld microstructure. There are several heat affected zones near the weld pool, which has different properties and microstructure due to different cooling rates and heat flux orientation during solidification. Since welding is a complex multiphysical process there are various parameters such as electric current, oxygen presence, heat flow and weld pool flow which influence the quality of welding joint and efficiency of the process. In this paper we aim to experimentally and theoretically investigate how to modify heat and mass transfer in the weld pool and heat affected zone by static magnetic fields. Electromagnetic force is one of the ways how to affect the weld pool flow and to influence the heat and mass transfer from the weld pool to the base metal. Our research demonstrates that moderate DC magnetic field can cause various effects on the post-weld morphology, depending on the magnetic field direction. Analytical estimates and similarity analysis for high heat input welding on EH36 shipbuilding steel shows that electromagnetic methods, like application of DC magnetic field can be promising approach for improved welding outcome in some cases.

Insights into the response of anammox process to oxytetracycline: Impacts of static magnetic field

The long-term effects of oxytetracycline (OTC) with a high concentration on the anaerobic ammonium oxidation (Anammox) process were evaluated, and the role of static magnetic field (SMF) was further explored. The stress of OTC at 50 mg/L had little effect on the nitrogen removal of anammox process at the first 16 days. With the continuous addition of OTC and the increase of nitrogen loading, the OTC inhibited the nitrogen removal and anammox activity severely. During the 32 days of recovery period without OTC addition, the nitrogen removal was further deteriorated, indicating the inhibition of OTC on anammox activity was irreversible and persistent. The application of SMF alleviated the inhibition of OTC on anammox to some extent, and the specific anammox activity was enhanced by 47.1% compared to the system without SMF during the OTC stress stage. Antibiotic efflux was the major resistance mechanism in the anammox process, and tetA, tetG and rpsJ were the main functional antibiotic resistance genes. The addition of OTC weakened the metabolic interactions between the anammox bacteria and the symbiotic bacteria involved in the metabolism of cofactors and secondary metabolites, leading to the poor anammox activity. The adaptability of microbes to the OTC stress was improved by the application of SMF, which can enhance the metabolic pathways related to bacterial growth and resistance to environmental stress.

Application of high-intensity static magnetic field as a strategy to enhance the fertilizing potential of sewage sludge digestate

Anaerobic digestion (AD) is a sustainable and well-established option to handle sewage sludge (SS), as it generates a methane-rich biogas and a digestate with potential fertilizing properties. In the past, different strategies have been proposed to enhance the valorization of SS. Among these, the application of a static magnetic field (SMF) has been poorly evaluated. This study aims to determine the effects of a high-intensity SMF (1.5 and 2 T) on the chemical composition of SS anaerobic digestate. Several strategies (i.e., number of magnetization cycles, addition of different sources and quantities of magnesium, and digestate aeration) have been applied to evaluate the possible formation of compounds with valuable fertilizing properties in the digestate. Experimental results showed that by combining different strategies promoting digestate exposure to the magnetic field it is possible to favour the reduction in the liquid phase of NH4+, NO3−, PO43−, SO42− and Mg2+ concentrations up to 28%, 38%, 34%, 39% and 31%, respectively. The XRD analyses conducted on the solid phase of the same magnetized digestate samples showed an increase in crystalline and amorphous phases of nitrogen and phosphorus compounds with fertilizing value, such as struvite. These results highlight that SMF application can increase the fertilizing potential of sewage sludge digestate and promote its valorization in a sustainable and circular perspective.

Design of 3D printed stage for in situ magnetic field application in magnetic force microscopy

We propose a design of 3D printed magnetic stage that allows application of static magnetic fields during magnetic force microscopy measurements. The stage utilizes permanent magnets providing spatial homogeneous magnetic fields. The design, assembly, and installation are described. Numerical calculations of the field distribution are used to optimize the size of magnets and the spatial homogeneity of the field. The stage offers a compact and scalable design, which can be adapted as an accessory onto several commercially available magnetic force microscopy platforms. The stage’s utility for in situ magnetic field application during magnetic force microscopy measurements is demonstrated on a sample of thin ferromagnetic strips.

Effect of static magnetic field assisted freezing on the product quality of Korla fragrant pear

AbstractFreezing is a good method of preserving the sensory and nutritional quality of fresh fruits and vegetables. However, big ice crystals always form in a common freezing process which causes mechanical damage to the fruit and vegetable cells and results in low‐quality products. The external magnetic field can interfere with the state of the internal water, thus changing the ice crystal formation process accordingly. It was suggested that suitable magnetic field treatments can affect the biological characteristics of fruits and vegetables and improve the quality of the frozen products. In this experiment, Korla fragrant pear was freezing processed at the different static magnetic field (SMF) strengths (0, 2, 4, 6, 8, and 10 mT), and the pear quality was evaluated by juice loss, color, texture, total soluble solid content and total acid content, water state, and distribution. The results showed that the utilization of SMF in freezing reduced the loss of juice and soluble solids, and maintained the titratable acidity. However, SMFs performed poorly in color and texture protection. This research suggested that magnetic field‐assisted freezing had some positive effects on the quality of frozen Korla fragrant pear, but more works need to be done to further improve the product quality.Practical applicationsThis research emphasizes the positive effect of static magnetic field treatment on improving the freezing rate and optimizing the freezing process of Korla fragrant pear, which also provides a new idea and practical reference for the potential application of static magnetic field in freezing preservation of fruits and vegetables.

Effectuating tunable valley selection via multiterminal monolayer graphene devices

Valleytronics using two-dimensional materials opens unprecedented opportunities for information processing using a valley polarizer as a basic building block. Various methodologies, such as strain engineering, the inclusion of line defects, and the application of static magnetic fields, have been widely explored for creating valley polarization. However, these methods suffer from low transmission or lack of polarization directionality. To overcome the above limitations, we propose an all-electrical valley polarizer using zigzag edge graphene nanoribbons in a multiterminal device geometry. The proposed device can be gate-tuned to operate along two independent regimes: (i) a terminal-specific valley filter that utilizes band-structure engineering, and (ii) a parity-specific valley filter that exploits the parity selection rule in zigzag edge graphene. We show that the device exhibits intriguing physics in the multimode regime of operation affecting the valley polarization; we investigate various factors influencing polarization in wide-device geometries, such as optical analogs of graphene Dirac fermions, angle-selective transmission via $p\ensuremath{-}n$ junctions, and the localization of edge states. Furthermore, we evaluate the performance of the proposed device structures in the presence of Anderson short-range disorder at the edges and the bulk, and we find it to be resilient to edge disorder even for a higher disorder strength. The device geometry is optimized to achieve maximum valley polarization, thereby paving the way for a physics-based tunable valleytronic device.

PROTOCOLO DE BIOMAGNETISMO MEDICINAL NO TRATAMENTO DE INSUFICIÊNCIA VENOSA CRÔNICA DE MEMBROS INFERIORES

Magnetic therapies have been the focus of studies due the positive results observed in clinical practice. The application of Static Magnetic Fields affects human body cells. This interaction has shown good results in the treatment of various symptoms and diseases. Based on studies by Dr. Richard Broeringmeyer, Dr. Isaac Goiz Durán developed a new therapy: Medicinal Biomagnetism (BM). Changes in body flows, such as the vascular change, is the base of one of Dr. Goiz findings and can be influenced by Biomagnetic Pairs that can sustain pathogenic microorganisms or generate tissue changes that can hinder venous return. Chronic Venous Insufficiency (CVI) is the deficiency of blood flow in the deep veins of the lower limbs, resulting from dysfunctions of venous return from the limbs to the heart, causing blood reflux, venous hypertension sustained by blood accumulation in the deep veins. BM is a non-invasive, low-cost, painless, easy-to-apply and minimally contraindicated complementary practice, which aims the prevention of various pathologies, including CVI, as an alternative with great therapeutic potential. The purpose of this bibliographical review study of an exploratory and qualitative analytical method is to present a BM protocol that can be applied to assist in CVI`s treatment. As a result of this study, the Treatment Protocol for Lower Limbs Blood Flow (FSMI) is presented. It concludes that this might help in CVI`s treatment, signs and symptoms, facilitating venous return, deserving to be measured in larger studies.

MEDICAL BIOMAGNETISM AND DETOXIFICATION

Medical Biomagnetism (MB) is an integrative technique developed by Isaac Goiz Durán. This technique involves the application of Static Magnetic Fields (SMF) on bioelectromagnetic dysfunctions called Biomagnetic Pairs (BMP). The technique identifies pH imbalances in anatomical areas of the body in order to correct them through the application of medium-intensity magnets. In this way, it influences the electrical and biochemical activity of the cell membrane aiming to restore metabolic balance benefiting the elimination of toxins through the body. It leads to prevent and recover Normal Energy Level (NEL) promoting homeostasis. We are increasingly exposed to large amounts of toxins from natural and processed foods, medications, environmental pollutants which overload our organ systems. The body is responsible for detoxifying naturally through cellular metabolism. However, when the body has supra-physiological levels of toxins, this system becomes weakened for this function presenting symptoms. The aim of this study is to present a protocol for MB detoxification of the human body, through descriptive, qualitative, and narrative literature review. A MB detoxification protocol was selected which can be applied by healthcare professionals, therapists, and self-applied as a possible complement to allopathic medicine deserving to be studied.

Enhancing anammox nitrogen removal by static magnetic field exposure: Performance, microbial community and symbiotic relationship analysis

Anammox batch reactors were operated with different static magnetic field (SMF) intensities (0 mT, 40 mT, and 100 mT). The impacts of SMF and the influent nitrogen concentration on nitrogen removal were analyzed. Sludge characteristics, microbial community structure, and metabolism pathways were explored. The results show that a high nitrogen load greatly decreased the nitrogen removal efficiency. Still, applying SMF with appropriate intensity enhanced the nitrogen removal performance, especially under high nitrogen loading conditions. Systems with 40 mT SMF at normal nitrogen loading conditions had the highest total nitrogen removal efficiency. In contrast, a higher SMF intensity was conducive to the anammox process with a high nitrogen load. Candidatus Kuenenia was fast enriched under high nitrogen loading conditions in the control systems. However, Candidatus Brocadia was more abundant than Ca. Kuenenia in the systems with SMF application. SMF was the main factor affecting the microbial metabolism, enhancing the metabolic pathways related to bacterial growth and resistance to environmental stress. The metabolic interactions between the anammox bacteria and other bacteria were enhanced by applying SMF to the anammox process, especially for the cross-feeding of cofactors and vitamins in the anammox consortia.

The best location for the application of static magnetic fields based on biokinetic coefficients in complete-mix activated sludge process

The use of the kinetic coefficients for the mathematical expression of the biochemical processes and the relationship between the effective parameters is importance. Change of the biokinetic coefficients in the complete-mix activated sludge processes were calculated for 1 month operation of the activated sludge model (ASM) in a Lab-scale in three series. 15 mT intensity of static magnetic fields (SMFs) applied on the aeration reactor (ASM 1), clarifier reactor (ASM 2) and, sludge returning systems (ASM 3) for 1 h, daily. During the operation of the systems, five basic biokinetic coefficients such as maximum specific substrate utilization rate (k), heterotrophic half-saturation substrate concentration (Ks), decay coefficient (kd), yield coefficient (Y) and, maximum specific microbial growth rate (μmax) were determined. The rate of k (g COD/g Cells.d) in ASM 1 was 2.69% and, 22.79% higher than ASM 2 and, ASM 3. The value of Ks (mg COD/L) was 54.44 and, 71.13 (mg/L) lower than the ASM 2 and, ASM 3. The rate of kd ASM 1, ASM 2 and, ASM 3 was 0.070, 0.054 and, 0.516 (d−1). The value of Y (kg VSS/kg COD) in ASM 1 was 0.58% and, 0.48% lower than ASM 2 and, ASM 3. The rate of μmax (d−1) in ASM 1 was 0.197, this value for ASM 2 and ASM 3 were 0.324 and 0.309 (d−1). Related to the biokinetic coefficients analyses the best location for the application of 15 mT SMFs was the aeration reactor, where the present of oxygen, substrate and, SMFs have the greatest impact on the positive changes of these coefficients.

A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis.

To investigate the therapeutic effects of static magnetic field (SMF) and its regulatory mechanism in the repair of osteoarthritic cartilage. Fourteen-week-old female C57BL/6 mice were randomly divided into the sham operation group and the osteoarthritis (OA) groups with and without SMF application. SMF was applied at 200​mT for two consecutive weeks. Changes in knee cartilage were examined by histomorphometry, and the chondrogenesis and migration of endogenous stem cells were assessed. The expression of SRY-related protein 9 (SOX9), Collagen type II (COL2), matrix metallopeptidase 13 (MMP13), stromal cell-derived factor 1/C-X-C chemokine receptor type 4 (SDF-1/CXCR4), Piezo1 and other genes was evaluated, and the mechanism of SMF's action was tested using the CXCR4 inhibitor, AMD3100, and Piezo1 siRNA. SMF significantly decreased the OARSI scores after induction of OA. SMF was beneficial to chondrogenesis by elevating SOX9. In the OA mouse model, an increase in MMP13 with a decrease in COL2 led to the destruction of the cartilage extracellular matrix, which was suppressed by SMF. SMF promoted the migration of cartilage-derived stem/progenitor cells and bone marrow-derived mesenchymal stem cells (MSCs). It increased SDF-1 and CXCR4, while the CXCR4 inhibitor significantly suppressed the beneficial effects of SMF. The application of Piezo1 siRNA inhibited the SMF-induced increase of CXCR4. SMF enhanced chondrogenesis and improved cartilage extracellular matrices. It activated the Piezo1-mediated SDF-1/CXCR4 regulatory axis and promoted the migration of endogenous stem cells. Collectively, it attenuated the pathological progression of cartilage destruction in OA mice. The findings in this study provided convincing evidence that SMF could enhance cartilage repair and improve OA symptoms, suggesting that SMF could have clinical value in the treatment of OA.

Effects of gradient high-field static magnetic fields on diabetic mice.

Although 9.4 T magnetic resonance imaging (MRI) has been tested in healthy volunteers, its safety in diabetic patients is unclear. Furthermore, the effects of high static magnetic fields (SMFs), especially gradient vs. uniform fields, have not been investigated in diabetics. Here, we investigated the consequences of exposure to 1.0-9.4 T high SMFs of different gradients (>10 T/m vs. 0-10 T/m) on type 1 diabetic (T1D) and type 2 diabetic (T2D) mice. We found that 14 h of prolonged treatment of gradient (as high as 55.5 T/m) high SMFs (1.0-8.6 T) had negative effects on T1D and T2D mice, including spleen, hepatic, and renal tissue impairment and elevated glycosylated serum protein, blood glucose, inflammation, and anxiety, while 9.4 T quasi-uniform SMFs at 0-10 T/m did not induce the same effects. In regular T1D mice (blood glucose ≥16.7 mmol/L), the >10 T/m gradient high SMFs increased malondialdehyde ( P<0.01) and decreased superoxide dismutase ( P<0.05). However, in the severe T1D mice (blood glucose ≥30.0 mmol/L), the >10 T/m gradient high SMFs significantly increased tissue damage and reduced survival rate. In vitro cellular studies showed that gradient high SMFs increased cellular reactive oxygen species and apoptosis and reduced MS-1 cell number and proliferation. Therefore, this study showed that prolonged exposure to high-field (1.0-8.6 T) >10 T/m gradient SMFs (35-1 380 times higher than that of current clinical MRI) can have negative effects on diabetic mice, especially mice with severe T1D, whereas 9.4 T high SMFs at 0-10 T/m did not produce the same effects, providing important information for the future development and clinical application of SMFs, especially high-field MRI.

Origin of static magnetic field induced quality improvement in sea bass (Lateolabrax japonicus) during cold storage: Microbial growth inhibition and protein structure stabilization.

To explore the potential application of static magnetic field (SMF) treatment in marine fish preservation, the sea bass (Lateolabrax japonicus) was exposed to SMF (5 mT) and its quality changes during cold storage were evaluated by total viable counts, water holding capacity, pH, color, and textural properties. Characteristics of the protein in the presence of SMF were investigated by measuring total sulfhydryl (SH) content, Ca2+-ATPase activity, secondary structure, and muscle microstructure. SMF treatment exhibited positive effects on fish quality, showing favorable performance on the most quality indicators, especially a significant reduction in the Microbial Counts. Furthermore, higher total SH content and Ca2+-ATPase activity were observed in SMF-treated samples, demonstrating that the oxidation and denaturation of myofibrillar protein (MP) were delayed due to SMF treatment. The transformation of α-helix to random coil was prevented in SMF-treated samples, indicating that the secondary structure of MP was stabilized by SMF treatment. The above changes in protein structures were accompanied by changes in muscle microstructure. More intact and compact structures were observed in SMF-treated samples, characterized by well-defined boundaries between myofibers. Therefore, our findings suggest that under the conditions of this article, SMF treatment could maintain the quality of fish mainly by inhibiting the growth of microorganisms and enhancing the stability of protein structures, and could be a promising auxiliary technology for preservation of aquatic products.