States In Peninsular Malaysia Research Articles

Strong association between high burden of soil-transmitted helminthiasis and household transmission among Malaysian Negritos, urging anthelmintic treatment beyond children

The Orang Asli (OA), Malaysia’s indigenous minority, continue to experience ongoing soil-transmitted helminth (STH) infections despite efforts including the Resettlement Programme (RPS). This study explores STH epidemiology and associated risks of moderate-to-heavy infections among 259 indigenous Negritos across four states in Peninsular Malaysia. Stool samples, anthropometrics, haemoglobin levels (finger-prick) and sociodemographic and environmental-sanitation factors via pretested questionnaires were collected. Parasitological examinations (direct faecal smear, formol-ether concentration, and Kato-Katz techniques) were thoroughly performed. The study revealed a 63.3% overall STH prevalence, with Trichuris trichiura (57.9%) being the most common, followed by Ascaris lumbricoides (35.5%) and hookworm (8.9%). Moderate-to-heavy infections were observed in 39.3% for T. trichiura and 48.9% for A. lumbricoides, with mixed infections of these two species being the most prevalent (50.6%). The risk for moderate-to-heavy infection increased significantly if other household members were infected (P < 0.001). Adults and adolescents also showed high infection rates, with no significant differences with children’s groups. These findings emphasize the need for anthelmintic treatment across all age groups and strategize local policies targeting older groups of the OA community to control ongoing transmission and reduce the STH burden and protect the future well-being of OA children, who deserve a healthier future.

Two new species of Austrelatus diving beetles from continental Southeast Asia, linking the distribution area of the genus (Coleoptera: Dytiscidae: Copelatinae)

Two new species of predominantly Australasian genus Austrelatus Shaverdo et al., 2023 are described and illustrated: Austrelatus mirai sp. nov. from Selangor State in Peninsular Malaysia is similar in habitus to the South Indian species A. boukali (Hendrich &amp; Balke, 1998) and A. davidi (Wewalka, 2017); however, the shape of the male genitalia suggests that it is closely related to Austrelatus species from Kalimantan. Austrelatus riberai sp. nov. from Shan State in Myanmar is most similar to Sino-Japanese A. parallelus (Zimmermann, 1920). Findings of Austrelatus species in continental Southeast Asia fill the gap in the distribution area of the genus between India, China and Japan on one side, and the Sunda Islands on the other side.

Rare occurrence of Blastocystis in sea turtles and insects (cockroaches, houseflies, and crickets) from several states in Peninsular Malaysia.

Blastocystis a single-celled eukaryotic protist, is known to inhabit the intestines of various hosts, including humans, and has been implicated in a wide spectrum of symptoms, ranging from gastrointestinal issues to skin disorders, thereby establishing its status as an emerging infectious agent. In this study, the prevalence of Blastocystis infection was investigated in insects, including cockroaches, houseflies, and crickets, as well as sea turtles. Additionally, the genotypic characteristics of the isolated Blastocystis strains were examined, and the evolutionary relationships between Blastocystis species found in sea turtles, and animals/humans were determined. Microscopic techniques and molecular methods were utilized in this study. The results showed that four out of 90 insects (4.44%) and one out of 13 sea turtles (7.7%) were infected by Blastocystis. Furthermore, detailed observations revealed the presence of characteristic morphological features, such as vacuolar forms in the cockroach, cricket and sea turtle samples and binary fission from cockroach samples, indicative of Blastocystis' mode of reproduction. While the ST8 of Blastocystis in sea turtles were successfully identified, no subtyping was achieved for the infected insects. This study not only establishes the occurrence of Blastocystis infection in sea turtles but also uncovers its ability to infect insects, suggesting a potential reservoir role for these organisms. Overall, this research emphasizes the significance of comprehending the prevalence, genotypic diversity, and evolutionary relationships of Blastocystis across various hosts. Such insights are instrumental in developing effective control measures and public health interventions to mitigate the associated symptoms and prevent future outbreaks.

Prospective associations between an empirically derived dietary pattern and life satisfaction in Malaysian adolescents

BackgroundLittle is known on the prospective associations between an empirically derived dietary pattern (DP) and life satisfaction among adolescents. This PUTRA-Adol follow-up study aimed to assess the prospective associations between the empirically derived DP and life satisfaction during adolescence.MethodsA total of 585 and 262 adolescents participated in the baseline PUTRA-Adol study in 2016 and PUTRA-Adol follow-up study in 2019–2020, respectively. These adolescents were recruited from three southern states in peninsular Malaysia, namely Negeri Sembilan, Melaka and Johor. Dietary assessments were estimated using a validated food frequency questionnaire (FFQ) while a Multidimensional Students’ Life Satisfaction Scale (MSLSS) was used to measure life satisfaction at baseline in 2016 as well as at follow-up in 2019–2020. A DP characterised by foods high in free sugar and energy dense was identified using reduced rank regression (RRR), cross-sectionally at baseline and was reported elsewhere. Similar RRR analysis was used to find a DP that best explained the variation in response variables linked to poorer life satisfaction, including dietary energy density (DED), fiber density, and percentage of energy from total fat and sugar at the PUTRA-Adol follow-up study. Prospective relationship between adherence to the identified DPs and overall life satisfaction scores as well as its domains between 2016 and 2019–2020 were evaluated using generalized estimating equation models (GEE).ResultsA DP characterized by high intakes of percentage energy from sugar, fibre and low in DED and percentage energy from total fat was identified at the 2019–2020 follow-up. The identified DP explained 11% of total variations in the response variables and was characterized by high intakes of sweets, sugar sweetened beverages (SSB) and fruits. Female adolescents [67.6(8.9)] had a mean (SD) of life satisfaction that was higher than male adolescents [67.5(10.8)] p < 0.05 in 2019–2020. Prospective analysis found a significant positive association between the identified DP and school domain, in male adolescents (β = 0.117; 95% CI 0.001, 0.234) and an inverse relationship between the DP z-score and self-domain in all adolescents (β = − 0.060; 95% CI − 0.115, − 0.005) from 2016 to 2019–2020.ConclusionsAn increasing score for the predominant ‘High sugar and High fibre’ DP was prospectively associated with increasing life satisfaction score for the school domain in male adolescents and decreasing score for self-domain in both male and females during adolescence. A lifestyle intervention targeting reduced dietary intakes, particularly sugar, may improve life satisfaction in adolescents and promote healthier future without compromising dietary intakes for chronic disease prevention later in life.

Multicentre case–control study of pneumococcal infections among children with pneumonia in Peninsular Malaysia (MY-Pneumo): a study protocol

BackgroundS. pneumoniae (SPN) is the most common cause of pneumonia. The disease can be effectively prevented through immunisation. Since December 2020, the Malaysian Government has included the 10-valent pneumococcal conjugate vaccine (PCV10) for all infants born on or after 1 January 2020 as part of the National Immunisation Programme (NIP). However, the epidemiology of pneumonia remains poorly understood. To fill the knowledge gap, we established a multicentre surveillance study to understand the burden of pneumococcal pneumonia among young children in Peninsular Malaysia.MethodsMY-Pneumo is a multicentre prospective case–control study conducted in three sentinel sites located in three different states of Peninsular Malaysia – Kuala Lumpur, Pahang, and Kelantan. A cohort of at least 500 incident cases and 500 controls is enrolled beginning in October 2021 and matched for age. Cases are hospitalised children < 5 years with radiologically confirmed pneumonia, and the controls are children without any features suggestive of pneumonia. Clinical samples, including nasopharyngeal swabs (NPS) and urine, are collected according to the study protocol. Biological fluids such as blood, cerebrospinal fluid (CSF) and pleural fluid are obtained from invasive pneumonia disease (IPD) patients, if available. All children are tested for SPN using polymerase chain reaction (PCR) and pneumococcal urine antigen test (PUAT) using BinaxNow.DiscussionSurveillance data, including carriage rate, serotype variations and the phylogeny data structure of SPN among young children in Malaysia during PCV implementation, will be generated from this study. Trends and patterns of pneumococcal serotypes by different regions are important for targeted public health strategies. Our data will provide baseline information for estimating the impact of PCV10 implementation and will influence policymakers' decisions regarding the upgrade from PCV10 to a higher-valency conjugate vaccine in Malaysia.Trial registrationThis project was registered at ClinicalTrials.gov (NCT04923035) on 2021, June 11. The study protocol was approved by the International Medical University Joint-Committee on Research & Ethics (4.15/JCM-216/2021) and the Institutional Review Board at sentinel sites (USM/JEPeM/21020190, IREC 2021–114, MREC ID No: 2021128–9769) and University of Southampton's Ethics and Research Governance (ERGo II 64844).

A randomised controlled trial comparing online versus face-to-face smoking cessation course for Malaysian dental officer

Oral health professionals play a vital role in tobacco cessation efforts, but there is a notable gap in their training and skills in this area. Online Smoking Cessation Course was developed by UiTM to benefit the oral health professionals however it has not been evaluated yet. Thus, the aim of this study is to evaluate the effectiveness of online smoking cessation module in comparison with the face-to-face method, in term of the level knowledge. This randomized controlled trial involved 185 new dental officers from four states in Peninsular Malaysia, divided into online and face-to-face (F2F) delivery groups. They were assessed using validated questionnaire, both pre- and post-intervention. The results showed that both the control (F2F) and intervention (online) groups demonstrated significant improvements in knowledge post-training. The control group's average score increased from 6.05 to 7.99, while the intervention group saw an increase from 6.22 to 8.20, with both groups showing statistically significant improvements (p&lt; 0.001). This indicates the effectiveness of the smoking cessation course in enhancing knowledge. The study concluded that the online module is suitable for new dental officers, equipping them with knowledge and skills for smoking cessation counselling as it found that online delivery is as effective as F2F training. This suggests that online training could provide a more cost-effective and accessible method for future smoking cessation training, reaching a broader audience.

Seroprevalence and contributing factors of transboundary animal diseases in sheep and goats: a study in Peninsular Malaysia.

Diseases caused by small ruminant lentiviruses, Mycobacterium avium ssp. paratuberculosis (MAP), Schmallenberg virus, and peste des petits ruminants virus (PPR) is globally recognised as serious threats to the ruminant industry due to their potential to spread rapidly across boundaries. Despite their global distribution and negative impacts on ruminant production, there is a gap in knowledge of the current trends in their epidemiology among sheep and goat populations in Peninsular Malaysia. This study was therefore designed to fill the gap of knowledge concerning the seroprevalence and contributing factors of CAEV, paratuberculosis, SBV, and PPRV among small ruminants from selected flocks in Selangor, Negeri Sembilan, and Pahang states in Peninsular Malaysia. A cross-sectional study design was used to collect animal data and blood samples for serological assays simultaneously. The ID Screen (ID.VET, France) indirect ELISA screening tests were used to detect serum antibodies directed against CAEV/MVV (VISNAS Ver 0922), paratuberculosis (PARAS Ver 0516), SBV (SBVC Ver 1114) and PPRV (PPRC Ver 0821). There was 45.4% (95% CI = 40.74-50.74), 6.8% (95% CI = 4.66-9.69), 27.8% (95% CI = 23.35-32.77), and 2.6% (95% CI = 1.11-0.51) true seroprevalence for CAEV, paratuberculosis, SBV, and PPR, respectively. Geographical location and species were the risk factors for CAEV and paratuberculosis, while the management system and age of small ruminants were the risk factors for SBV. The present study is the first to document a large-scale seroprevalence of MAP and PPR infection among sheep and goat flocks in Peninsular Malaysia. The presence of PPRV and MAP antibodies among small ruminant flocks is signalling current or previous exposure to the pathogens or cross reactions with similar antigens. This finding further suggests the potential for future outbreaks of these devastating diseases among sheep and goats in Malaysia. The high seroprevalence of CAEV and SBV among small ruminants indicates high levels of exposure to the viruses in the environment, which is a potential threat to production.

Normalized Difference Vegetation Index (NDVI) marked reliable value of Ulu Muda Forest Reserve

Water resources must be preserved because they are vital for agricultural, industrial, and domestic use, especially in tropical forest regions. Given the importance of the Ulu Muda Forest Reserve to the Kedah state in sustaining paddy cultivation for the country, this study aimed to provide reliable forest classification method and produce Normalized Difference Vegetation Index-Maximum Likelihood Classification of forest density map for Ulu Muda Forest Reserve. Here, the study reports measurements of the satellite remote sensing index for the forest based the image date. The forest recorded NDVI index of 0.72 to 0.98 for high density, NDVI index of 0.61 to 0.71 for moderate and NDVI index of 0.26 to 0.60 for low density area. The index showed higher value, which is comparable with reports of other tropical forest elsewhere. The results were influenced using satellite remote sensing pre-processing, processing, and classification. As a result, based on the satellite image indices approach, upper threshold of the higher density, the forest can exhibit higher capability for sustaining its ecological and hydrological functions. Given that most of the tropical forest biome has sustained this threshold, forest management should be sustained at this state, and more measures should be taken for example increase of protection forest classification in Rancangan Pengurusan Hutan (RPH) that is one of yearly planning report in states of Peninsular Malaysia. In the meantime, Geographical Information System (GIS) capacity should be mandate looking forwards for more utilisation in mapping for planning and projection of forest resource management activities.

The prevalence of simian malaria in wild long-tailed macaques throughout Peninsular Malaysia

The parasite Plasmodium knowlesi has been the sole cause of malaria in Malaysia from 2018 to 2022. The persistence of this zoonotic species has hampered Malaysia’s progress towards achieving the malaria-free status awarded by the World Health Organisation (WHO). Due to the zoonotic nature of P. knowlesi infections, it is important to study the prevalence of the parasite in the macaque host, the long-tailed macaque (Macaca fascicularis). Apart from P. knowlesi, the long-tailed macaque is also able to harbour Plasmodium cynomolgi, Plasmodium inui, Plasmodium caotneyi and Plasmodium fieldi. Here we report the prevalence of the 5 simian malaria parasites in the wild long-tailed macaque population in 12 out of the 13 states in Peninsular Malaysia using a nested PCR approach targeting the 18s ribosomal RNA (18s rRNA) gene. It was found that all five Plasmodium species were widely distributed throughout Peninsular Malaysia except for states with major cities such as Kuala Lumpur and Putrajaya. Of note, Pahang reported a malaria prevalence of 100% in the long-tailed macaque population, identifying it as a potential hotspot for zoonotic transmission. Overall, this study shows the distribution of the 5 simian malaria parasite species throughout Peninsular Malaysia, the data of which could be used to guide future malaria control interventions to target zoonotic malaria.

Anticancer agents and genetic identification of Pterospermum, an indigenous plant of Sarawak, Malaysia

In Malaysia, Pterospermum is distributed in the states of Peninsular Malaysia, Sarawak, and Sabah. For centuries, the Bidayuh tribe in Sarawak has been using an indigenous plant to cure patients diagnosed with breast cancer. The plant is called Bayur by the tribe, which is the similar local name known for several Pterospermum species in Sarawak, Sabah, and Indonesia. This study was carried out to genetically identify the indigenous plant using the core barcode loci, rbcL and matK, as well as profiling anticancer agents from this plant. Phylogenetic analyses based on both rbcL and matK loci did not show clear species resolution for the indigenous plant, however, the analyses showed consistent relationships among congeneric species of Pterospermum. Interestingly, UHPLC-QQQ/MS analysis showed the presence of anti-cancer agents in the bark extract, thus supporting the potential of the indigenous plant for breast cancer treatment.

Assessing the effect of cardiovascular disease on work productivity and financial loss among school teachers in Peninsular Malaysia: a nested case-control study.

School teachers may have an increased risk of cardiovascular disease (CVD), potentially affecting their work productivity. However, limited data exists on the impact of CVD on teachers' productivity in Malaysia. Our objectives were to assess work productivity loss (absenteeism and presenteeism) as well as to determine the associated annual monetary loss among school teachers who experienced incident CVD in Peninsular Malaysia. We adopted a nested case-control design within a cohort of school teachers. Working teachers from six states of Peninsular Malaysia, and had experienced incident CVD before a right-censored date (31st December 2021) were defined as cases. Incident CVD was operationally defined as the development of non-fatal acute coronary syndrome (ACS), stroke, congestive cardiac failure, deep vein thrombosis or peripheral arterial disease before the censored date. Controls were working teachers who did not acquire an incident CVD before the similar right-censored date. All controls were randomly selected, with a ratio of one case to four controls, from among the working teachers in one of the states in Peninsular Malaysia. We used a shortened version of the Malay-validated World Health Organization-Health and Work Performance Questionnaire (WHO-HPQ) to estimate the workplace productivity effect among teachers with incident CVD (cases). The same questionnaire was distributed to teachers in a single state of Peninsular Malaysia who did not experience incident CVD (controls). Absenteeism, presenteeism and annual monetary loss were computed based on the scoring rules in the WHO-HPQ. Analysis of covariance was performed with covariate adjustment using propensity scores. The bootstrapping method was applied to obtain better estimates of marginal mean differences, along with standard errors (SE) and appropriate effect sizes. We recruited 48 cases (baseline mean age = 42.4 years old, 54.2% females) and 192 randomly selected controls (baseline mean age = 36.2 years old, 99.0% females). The majority of the cases had ACS (73.9%). No significant difference was observed in absenteeism between cases and controls. The mean self-rated job performance score was lower for cases (7.63, SE = 0.21) compared to controls (8.60, SE = 0.10). Marginal mean scores of absolute presenteeism among cases (76.30) were lower (p<0.05, eta squared = 0.075) than controls (85.97). The marginal mean annual cost of presenteeism was higher in cases (MYR 21,237.52) compared to controls (MYR 12,089.74) (p<0.05, eta squared = 0.082). Absolute presenteeism was lower among school teachers who experienced incident CVD and the annual cost of presenteeism was substantial. Implementing supportive work strategies in school settings is recommended to increase absolute presenteeism, which can lead to a reduction in the annual cost of presenteeism among teachers experiencing incident CVD.

Dairy farmers' knowledge, awareness and practices regarding bovine lameness in Malaysian dairy farms.

Given the data paucity on dairy farmers' perspectives regarding bovine lameness and hoof diseases, particularly in South East Asian countries, this study was conducted to assess the knowledge, attitude and practices toward lameness and hoof health among dairy cattle farmers in Malaysia. An online-based and face-to-face survey was conducted among 114 dairy farmers from four states in Peninsular Malaysia. Data were analysed using descriptive statistics, principal component analysis and an independent sample t-test. Overall, farmers demonstrated satisfactory knowledge and attitude regarding lameness and its impact on dairy cattle welfare and production. Lameness was ranked the second most important health issue in dairy farms after mastitis. Notably, 90% reported the presence of at least one lame cow on their farms, and 55% stated lameness as the reason for culling their cows. While sole ulcer was the hoof lesion mostly identified by farmers, 75% of them underestimated lameness prevalence on their farms and rarely implemented management strategies such as preventive hoof trimming and footbath. Farmers' educational qualification influenced their understanding of the impact of lameness on dairy cattle production. Despite reflecting satisfactory knowledge and attitude towards lameness in dairy cows, farmers in this study need to improve their current management practices to address lameness problem in their herds. Educating farmers on the importance of early detection and prompt treatment, and preventive measures are crucial for lameness control and improving hoof health in these dairy farms.

The Ongoing Battle: Understanding the Population Trends of Green Turtles in Peninsular Malaysia

Sea turtles are crucial marine animals. They act as indicator to a healthy marine ecosystem. However, they are subjected to different challenges and threats which results of their declining population. This study looks at the population trends of sea turtles in Malaysia by examining the numbers of nests, eggs, and hatchlings in six states in Peninsular Malaysia which are Perak, Pahang, Melaka, Johor, Terengganu, and Negeri Sembilan. Statistical distribution, hatching success percentage, trend landing and hatching, and linear regression analysis are implemented. The results show that Terengganu, on the east coast, consistently has the highest numbers of nests, eggs, and hatchlings, highlighting its key role in sea turtle conservation. In contrast, Melaka has the lowest numbers, pointing where conservation efforts could be increased. This study provides valuable insights into the differences in green turtle populations across states and identifies critical areas for focused conservation strategies to better protect and help these endangered species recover in Malaysia.

Screening of Streptococcus suis in swine workers of selected states in Peninsular Malaysia.

Streptococcus suis is a zoonotic pathogen that is highly associated with contact between live pigs and raw pig material. In view of the recent reports of human infections in Malaysia, epidemiological data on the status of S. suis in the human population, especially among people working closely with pigs and/or raw pork, should be provided. The aim of this study was to detect S. suis among individuals working in the swine industry in several major pig production areas in Peninsular Malaysia. Demographic information, exposure determinants, and oral swabs were collected from swine personnel, including farmers, butchers, and veterinarians. Oral swabs were subjected to bacterial isolation and conventional polymerase chain reaction (PCR) assays for S. suis detection. The study included 40 participants working in the swine industry, with a predominant representation of males (62.5%) and Malaysian Chinese individuals (60.0%) who consumed pork (92.5%). Notably, none of the participants reported consuming raw or partially cooked pork. In spite of their occupational exposure risk, none of the oral swabs showed positive results for S. suis infection. To the best of our knowledge, this is the first report and detection study of S. suis using oral swabs obtained from swine personnel in Peninsular Malaysia.

COMMUNITY-BASED EYE HEALTH SCREENING REPORT IN MALAYSIA: URBAN-RURAL REFERRAL COMPARISON

This study compared urban and rural referrals from a community-based eye health screening program named 'Jom Check Mata Malaysia (JCMM)'. The program was carried out in nine different states in Peninsular Malaysia—a total of 18 screening locations with a mix of urban and rural. Two mobile eye clinics were mobilised for community-based eye health screenings. This study involved 3,139 participants (1,158 and 1,981 participants from rural and urban, respectively). Visual complaints were collected via interviews using an ocular symptom checklist. The visual acuity was screened using a Snellen Chart. Ocular health screening, including anterior and posterior eye segments, was screened using slit-lamp bio-microscopy and fundus camera. Overall referrals were 2,139 (68%), 846 (27%) and 362 (12%) based on visual complaints, visual acuity and ocular health screening, respectively. Rural locations had significantly higher referral rates for visual complaint screening (χ2 = 422.53, p&lt;0.05), visual acuity screening (χ2 = 20.20, p&lt;0.05) as well as ocular health screening (χ2 = 64.70, p&lt;0.05) compared to the urban locations. There was a consistent pattern of higher rural referrals for all types of vision screening. Information from our three-category screening analysis could help to design a vision screening for our rural and urban populations.

Changes in Kidney Function Among Malaysian Adolescents and Its Determinants

The health and wellbeing of adolescents are often neglected, including the knowledge of chronic kidney disease (CKD), especially in its early stages. A total of 607 adolescents were recruited from the Malaysian Health and Adolescents Longitudinal Research Team (MyHeART) study, a prospective cohort study conducted from March 2012 to May 2016 that explored the noncommunicable diseases (NCDs) risk factors among 13 to 17 years old students in 3 states of Peninsular Malaysia. Students who participated in all 3 data collection periods in 2012, 2014, and 2016 with kidney function assessment across all 3-time points were included in the current study. The students' estimated glomerular filtration rate (eGFR) was calculated from isotope-dilution mass spectrometry-traceable Schwartz's equation and categorized based on Kidney Disease: Improving Global Outcomes (KDIGO) classification. Changes in kidney function were examined, and the longitudinal relationship between eGFR and multiple NCD risk factors was analyzed using the generalized estimating equation (GEE). The prevalence of decreased eGFR (60-89 ml/min per 1.73 m2) among the students increased from 6.1% (2012) to 30.0% (2014) and 40.2% (2016). Based on the GEE, the student's eGFR decreased over time, with a steeper decline during early to midadolescence. Males and rural students had lower eGFR compared to their counterparts. Students who are morbidly obese had lower eGFR than those with normal body mass index (BMI). Protein consumption also has a potential moderating effect on eGFR in adolescents. Kidney function changes can be detected as early as adolescence and are likely attributable to multiple NCD risk factors. Therefore, more comprehensive prevention efforts from various stakeholders are needed to identify health issues like CKD.

Comparative Metabolomics Analysis of Weedy Rice (Oryza spp.) across Peninsular Malaysia

Weedy rice (Oryza spp.) is a notorious weed that invades paddy fields and hampers the rice’s production and yield quality; thus, it has become a major problem for rice farmers worldwide. Weedy rice comprises a diverse morphology and phenotypic variation; however, the metabolome and chemical phenotypes of weedy rice grains have not been explored. Therefore, this study is aimed to investigate the metabolite profiles and chemical diversity of Malaysian weedy rice. Thirty-one biotypes of weedy rice grains were collected from selected rice granaries in different states of Peninsular Malaysia, including Selangor, Perak, Penang, Kedah, Perlis, Kelantan, and Terengganu. In addition to the weedy rice samples, four cultivated rice varieties (MR219, MR220, MR220 CL2, and MARDI Siraj 297) were subjected to nuclear magnetic resonance-based metabolomics. The PLS-DA and OPLS-DA models revealed a clear separation between the weedy rice and cultivated rice, which was contributed by the higher level of γ-aminobutyric acid (GABA), α-glucose, fumaric acid, and phenylalanine in the weedy rice, whilst valine, leucine, isoleucine, fatty acids, 2,3-butanediol, threonine, alanine, butyric acid, choline, γ-oryzanol, fructose, β-glucose, sucrose, ferulic acid, and formic acid were found dominant in the cultivated rice. Interestingly, the models also showed a separation between the weedy rice samples collected from the west coast and east coast regions of Peninsular Malaysia. The metabolites responsible for the separation, i.e., threonine, alanine, butyric acid, fructose, β-glucose, and formic acid, were found higher in the west coast samples, and the east coast samples were discriminated by higher levels of valine, leucine, isoleucine, fatty acids, 2,3-butanediol, choline, GABA, γ-oryzanol, α-glucose, sucrose, fumaric acid, ferulic acid, and phenylalanine. This study is the first to provide insights into the metabolite profiles and chemical phenotypes of Malaysian weedy rice that could be influenced by genotype and environmental conditions. The information on the weedy rice metabolome and omics data is important for further research on weed management and crop improvement.

IMPACT OF FOOT AND MOUTH DISEASE ON CATTLE PRODUCTION IN PENINSULAR MALAYSIA

Foot and mouth disease is vital in Malaysia with increasing yearly cases. Quantitative information about the impact of the disease on the cattle in the country, however, is scarce. This study quantitatively assesses the direct economic impacts of foot and mouth disease outbreaks on cattle production in Peninsular Malaysia. The impacts were assessed based on the data obtained from case outbreaks of cattle in 2017 and 2018. Data were collected using a questionnaire administered to 349 cattle farmers in six states of Peninsular Malaysia from April to October 2018. Collected data were analyzed using a t-test (i.e., to compare between gender within the age of breed) and one-way ANOVA (i.e., to compare between breeds and between ages within the breed). Results demonstrate that the breed of Limousine, Charolaise and Kedah-Kelantan have been proven affected due to the FMD outbreak. The economic losses of foot and mouth disease outbreaks due to mortality and weight loss reached the figure of MYR390.24 per herd. To reduce the impact of the loss of cattle production and achieve Malaysia food security target, it is vital for the government to surveillance and eradicate the FMD outbreak.

Analysis of issues and solutions on the implementation of istibdal waqf property in states of Peninsular Malaysia

Purpose This paper aims to analyse the implementation of istibdal waqf property in several states of Peninsular Malaysia. To achieve this objective, this study identified the amount of waqf property ‘am (general) and waqf property khas (special) which were involved in the process of istibdal in each state, the factors that have caused the istibdal to be implemented, the rate of istibdal involved for each lot of waqf lands and the type of property replacement performed. Design/methodology/approach This study used the qualitative method, whereby the researchers had collected secondary data consisting of documents related to waqf from the State Islamic Religious Council (MAIN). The type of documents obtained were public records. The data collected were analysed using the content analysis techniques. Findings The results showed that there are several factors involved in the implementation of istibdal, namely, the acquisition of waqf land by the State Authority, applications by external parties for a particular interest and the initiatives taken by the MAIN on waqf property which is problematic and uneconomical to generate waqf fund. This study also found some Shariah and management issues, which were identified in the implementations of istibdal, whereas some cases of istibdal were only carried out on a small part of the waqf lands and there were also cases of the implementation of istibdal which have not been replaced with fixed assets as authorised by the istibdal parameter. This study suggests some improvements to the issues identified in the implementation of istibdal for waqf managers in Malaysia. Research limitations/implications The limitation of this study is that it analyses only the documents that record the implementation of istibdal in the states managed by MAIN. Thus, the analysis performed was limited to the documents obtained without involving empirical data. Practical implications This study suggests some improvements to the implementation of istibdal waqf in the states studied. Therefore, these recommendations can be used by waqf property managers to improve the process of implementing waqf property istibdal so that it can be used to its maximum potential. Social implications If the recommendations in this study can be implemented, the Muslim community will benefit greatly from the waqf property because it is being developed through the method of istibdal. This impact can increase the confidence of the Muslim community towards MAIN in managing waqf property and encouraging the Muslim community to contribute to waqf property for the welfare of the ummah. Originality/value This study involved data on a larger waqf istibdal implementations that involved several states in Peninsular Malaysia, which to the best of the authors’ knowledge, the analysis involving such large research data have not been implemented before.

Assessments of Heavy Metals Accumulation, Bioavailability, Mobility, and Toxicity in Serpentine Soils

Accumulation of heavy metals is a concerning issue due to their known persistence in the ecosystem, and there are standard limits established for their maximum allowable concentrations in soils. However, heavy metal accumulation coming from serpentinite soils often exceeds the regulatory values, and there is a lack of knowledge regarding their bioavailability, mobility, and toxicity in the environment. This research applied novel selective sequential extraction and leaching procedures to assess the gaps in knowledge regarding heavy metals accumulation on serpentinite topsoil derived from a few states in Peninsular Malaysia. Based on the total digestion method, the concentration of all studied heavy metals except Mn exceeded the site screening levels issued by the Department of Environment, Malaysia (DOEM). The Geo-accumulation Index categorized Cr, Cd, Ni, and Co as extreme contamination and Cu, Pb, Zn, and Mn as unpolluted to moderate contamination. From the extraction results, Cd was found bounded 100% to a residual fraction. Meanwhile, Ni, Co, and Cr were mostly (≥92%) found to be bound to a residual fraction, with the remaining percentages distributed within non-bioavailable fractions (crystalline Fe oxides, poorly crystalline Fe oxides, and Mn oxides). Nevertheless, Cu, Pb, Zn, and Mn contaminants showed an increase (1–9%) in bioavailability and mobility fractions (soluble–exchangeable, surface-adsorbed, and organic matter) which pose a threat to the environment. The toxicity of the heavy metals greatly surpassed the DOEM standards; however, it was still below the global USEPA toxicity control. This research concluded that, even though the toxicity level of the topsoils had not exceeded the global toxicity limit, the accumulation of heavy metals in the serpentinite soils needs to be addressed due to its high concentration and its being potentially bioavailable and mobile in the environment.