Starch Paste Research Articles

낱장문서 메움용 접착제 활용 연구

A study on adhesives for document mending was conducted by blending pulgasari(funori) with wheat starch paste. Pulgasari is usually used for the ‘facing’ process in paper conservation. 7 types of samples, shinpul, gopul, pulgasari blended adhesives were prepared and were applied on Hanji and their effect was assessed and quantified with instrumental methods. Adding pulgasari was shown effective to lower the artifact’s acidity and to increase the document’s flexibility. Adhesive force and viscosity and the degree of greenness and yellowness tended to rise as the ratio of pulgasari blended became higher. In microscopic observation, it showed less void and thicker film as the pulgasari ratio became higher. In the document mending experiment, the higher the ratio of pulgasari, the less stain and less contraction were shown. Blending pulgasari with wheat starch paste can be used as an alternative method to overcome the drawbacks of gopul and shinpul in document mending.

Impact of hydrothermal treatments on the functional, thermal, pasting, morphological and rheological properties of underutilized yam starches

Impact of hydrothermal treatments on the functional, thermal, pasting, morphological and rheological properties of underutilized yam starches

Exploring starches from varied sorghum genotypes compared to commercial maize starch

AbstractSorghum has been lately attracted the attention for human consumption as a gluten‐free cereal rich in bioactive compounds. Sorghum starches isolated from brown (BRS305), red (BRS308), and white (CMSXS180) genotypes were compared to commercial maize starch, based on proximate composition, particle size distribution, microstructure, X‐ray diffraction, amylose and total starch content, solubility index, swelling power, pasting, and thermal properties. The carbohydrate content of sorghum starch samples ranged from 98.24 to 99.23 g/100 g (dry basis). The particle size distribution of the CMSXS180 sorghum genotype was very similar to commercial maize starch. The relative crystallinity values varied from 29.83% to 30.39%. The water solubility index and swelling power of sorghum starches were lower than those of maize starch. Sorghum genotypes paste profiles were similar, but greatly differed from maize showing the highest final viscosity (4042.0–4444.5 cP) compared to maize starch (3767.5 cP) and lower gelatinization enthalpy (∆H = 9–10.5 J/g) than maize starch (11.8 J/g). The results showed some distinct properties of sorghum starches when compared to maize starch, which may contribute to provide alternative uses, particularly in food products requiring very high viscosity and retrogradation.Practical ApplicationsSorghum pericarp is rich in bioactive compounds, which can be used as a main source of ingredients, for example, to the nutraceutical industry. The sorghum grain without pericarp (decorticated), used to produce purified starch (without chemical treatment), may contribute to the total use of this cereal by limiting chemical residues. In addition, sorghum starch has shown particular technological properties different from commercial maize starch, providing alternative uses in the food industry, for example, in products that require high viscosity from hot to cold processes.

Pasting and Rheological Properties of Starch Paste/Gels in a Sugar-Acid System.

This study was to investigate the impact of granule size, amylose content, and starch molecular characteristics on pasting and rheological properties of starch paste/gels in neutral (water) and sugar-acid systems. Normal rice starch (RS), waxy rice starch (WRS), normal tapioca starch (TS), and waxy tapioca starch (WTS) representing small-granule starches and intermediate-granule starches respectively, were used in the study. Impacts of granule size, AM content, and their synergistic effects resulted in different starch susceptibility to acid hydrolysis and interactions between starch and sucrose-water, yielding different paste viscosities in both systems. The high molecular weight (Mw¯) and linearity of amylopectin and amylose molecules increased the consistency of starch pastes. RS produced a stronger and more brittle gel than other starch gels in both neutral and sugar-acid systems. The results indicated the impact of the effect of granule size and amylose content on starch gel behaviors. Properties of waxy starch gels were mainly governed by amylopectin molecular characteristics, especially in the sugar-acid system. Adding sugar and acid had minor impacts on starch gel behaviors in the linear viscoelastic (LVE) region but were most evident in the nonlinear response regime of starch gels as shown in the Lissajous curves at large oscillatory strain.

Correlation between chain structures of corn starch and properties of its film prepared at different degrees of disorganization

This study investigated the relationship between the chain structure of corn starch and the properties of corn starch-based films formed with starch pastes with different degrees of disorganization (70, 80, and 90 °C). The degree of gelatinization, chain length distribution, amylose content, and molecular weight of the corn starch were determined by the water absorption index, ion chromatography, spectrophotometry, and gel chromatography, respectively. The thickness, surface roughness, solubility, water content, water vapor permeability, mechanical properties, and maximum thermal degradation rate of corn starch-based films formed with starch pastes with different degrees of disorganization were evaluated. The moisture content, thickness and surface roughness of films formed with the starch pastes decreased. At the same time, the solubility, elongation at break, water vapor permeability, and molecular weight distribution increased with increasing heat treatment temperature. The maximum thermal degradation rate and tensile strength of the corn starch-based films formed with the starch pastes decreased with increasing heat treatment temperature. The gradual decrease in the amylose content of corn starch-based films formed with starch paste with increasing heat treatment temperature led to a change in the performance of the corn starch-based films.

Mechanisms of the different effects of sucrose, glucose, fructose, and a glucose-fructose mixture on wheat starch gelatinization, pasting, and retrogradation.

The gelatinization, pasting, and retrogradation of starch influence texture, quality, and shelf-life attributes of many foods. The purpose of this work was to document the effects of a 50:50 glucose:fructose (glc:fru) mixture and sucrose solutions on these starch traits to provide a fundamental basis to explain the different texture and shelf-life attributes of baked goods formulated with these sugars. Differential scanning calorimetry, rapid visco analyzer, and oscillatory rheometry were used to quantify the effects of glucose, fructose, glc:fru mixture, and sucrose at different concentrations (0% to 60% w/w), on the gelatinization temperature, pasting, and retrogradation properties of wheat starch. Distinct differences were found between the effects of sucrose and those of the monosaccharides including the glc:fru mixture. Sucrose elevated Tgel and pasting temperature most and decreased other RVA parameters compared to the monosaccharides as concentration increased. Fructose and the glc:fru mixture promoted amylopectin retrogradation, while retrogradation was inhibited in sucrose and glucose solutions. The glc:fru mixture had similar effects on starch properties compared to fructose under static measurement conditions (DSC), and the effects were in between those of glucose and fructose under dynamic conditions when shear was applied (RVA and rheology). These effects are explained by the phase separation and/or solute partitioning of the monosaccharide constituents of the glc:fru mixture. Sugar solution physicochemical properties correlated strongly with starch gelatinization and retrogradation. The results substantiate the important relationship between sugar physicochemical properties and solution dynamics with starch thermal properties, which in turn affect the texture and structure of starch-containing food products. PRACTICAL APPLICATION: The quality attributes of starch-containing baked goods are influenced by how different amounts and types of sugars affect starch cooking properties. The underlying mechanisms of the different sugar effects involve solution viscosity, intermolecular hydrogen bonding, and phase separation. Substituting one sugar for another has less effect on these starch properties in products with lower sugar concentrations than in products with more sugar. Mixtures of sugars behave differently than single sugars in different conditions due to phase separation. Baked goods made with glucose:fructose mixtures in place of sucrose likely have higher amounts of gelatinized starch and increased firmness (i.e., staling or retrogradation) over time.

Effects of selenium solution on the crystalline structure, pasting and rheological properties of common buckwheat starch.

Selenium is an important element that affects human growth and development, and also affects the yield and quality of common buckwheat. In our study, two common buckwheat varieties were sprayed with different concentrations (0 g/hm2, 5 g/hm2, 20 g/hm2) of sodium selenite solution at the initial flowering period and the full flowering period, respectively, to determine the effects of selenium solution on the physicochemical properties of common buckwheat starch. With increasing selenium levels, the amylose content, peak viscosity, breakdown, relative crystallinity, pasting temperature and gelatinization enthalpy first decreased and then increased, while the transparency showed a trend of increasing and then decreasing. All samples exhibited a typical A-type pattern, while at high selenium level, the degree of short-range order of common buckwheat starches changed. From the rheological properties, it can be seen that the starch paste is dominated by elastic properties, while the low selenium treatment decreases the viscosity of the starch paste. These results showed that spraying different concentrations of selenium solutions at different periods significantly affected the physicochemical properties of common buckwheat starch.

Extraction and characterization of waxy and normal barley β-glucans and their effects on waxy and normal barley starch pasting and degradation properties and mash filtration rate

Interactions between β-glucan and starch influence the health benefits of barley-based foods and barley brewing performance. Here, we characterized β-glucans from waxy and normal barley varieties and compared the effects of different β-glucans on the pasting and degradation of waxy and normal barley starches as well as the filterability of mashes from unmalted waxy and normal barley. Waxy barley Zangqing18 β-glucan displayed more compact micrographic features, higher molecular weight, larger particle size, higher thermal decomposition temperature and lower rheological viscosity than normal barley Zangqing2000 β-glucan. β-Glucan not only significantly decreased the pasting viscosities of waxy and normal starches but also lowered the pasting temperatures and peak times of normal starch, likely by inhibiting granule swelling and disrupting the integrity of the continuous phase. β-Glucan also decreased in vitro digestion extent of starch and increased the resistant starch. The unmalted waxy barley had a mash filtration rate much faster than normal barley because starch and β-glucan in waxy barley were rapidly and completely digested and formed more open filter passages. The effects of β-glucan on starch properties varied with the types and contents of β-glucans, whilst the types of starches showed more significant effects. Chemical compounds studiedβ-Glucan (Pubchem CID: 439262); Amylopectin (Pubchem CID: 439207); Starch (Pubchem CID: 156595876).

Characterisation of Pasting, Structural and Volatile Properties of Potato Flour

Potato flour is an important raw material for potato staple food products; nevertheless, the quality and flavor vary significantly due to process changes. In this study, the physicochemical features of fresh and five different dehydration temperature potato samples, including the degree of starch gelatinization (DG), pasting, structure properties and volatile components, were compared to investigate the effect of hot air drying (HAD) on potato flour. The results showed that the degree of pasting, viscosity and volatile aroma components changed significantly with differences in drying temperature. With the increase in drying temperature, the gelatinization degree and peak viscosity of potato powder increased or decreased, the breakdown viscosity of HAD-50 was higher, the setback viscosity of HAD-90 was higher, while the crystallization zone of HAD-90 was destroyed due to the high temperature. The flavor components of potato flour are increased during processing due to lipid oxidation, Maillard reaction and thermal degradation. The level of aldehydes,3,5-Octadien-2-one and E,E)-3,5-Octadien-2-one gradually reduced as the processing temperature increased, while the content of furans grew and then decreased, nonanal and 2-Penty-l-Furan increased. Overall, lower HAD temperatures are beneficial for the quality and flavor of potato flour. The information presented here will be useful for the further development of potato flour products.

A study on the structural, physicochemical, rheological and thermal properties of high hydrostatic pressurized pearl millet starch

Starch in native form has limited application due to functional and physicochemical characteristics. To overcome these limitations, starch can be modified by non-thermal technologies such as high hydrostatic pressure (HHP). This study investigates high-pressure-induced gelatinization and the effect of this process on the structural, functional, morphological, pasting, thermal, physical and rheological properties of millet starch. The suspension of millet starch and water was pressurized at 200, 400 and 600 MPa for 10, 20 and 30 min to modify the starch in terms of structure, morphology, some physicochemical and rheological properties. Swelling strength and starch solubility decreased as a result of treatment with HHP. All treatments caused to increase in water holding capacity of the starch (from 0.66 % for native starch to 2.19 % for 600 MPa-30 min). Thermal analysis showed a decrease in gelatinization temperature and enthalpy of gelatinization and the pasting properties showed a decrease in the peak viscosity after HHP treatment. In addition, HHP treatment caused to increase in the hydration ability of starch by creating porosity and gaps in the granule surface and increasing the specific surface area. HHP application resulted in an increase in the peak time and pasting temperature and a decrease in breakdown and peak viscosities, final viscosity and setback viscosity in comparison with native starch of millet. The starch sample treated with 600 MPa for 30 min had the lowest syneresis and retrogradation ability. Increasing pressure and the time led to an increase in the elastic nature of the starch samples. According to the results, it is possible to increase usage area of starches in the food industry by improving its technological with HHP. This green physical technology can influence the quality parameters of starch, which can provide benefits for product machining and economic purposes.

Chemical Modifications of Normal and Waxy Potato Starches Affect Functional Properties of Aerogels.

Aerogels are of increasing interest because of their exceptionally large surface area, porous structure, and low weight. Despite the significant increase in interest in the subject of starch-based aerogels, the number of detailed studies is rather scarce, which is especially evident in the case of chemically modified derivatives. Therefore, the study aims to evaluate the physicochemical properties of aerogels from chemically modified potato starch preparations (E 1422 and E 1450) obtained both from normal and waxy starches. Aerogels were prepared through the retrogradation of starch pastes followed by the gradual replacement of water with ethyl alcohol. The obtained preparations were characterized in terms of their bulk density, oil-binding capacity, as well as the texture and rheological properties of the formed pastes. Moreover, their usefulness was evaluated in an emulsion system employing rheological and low-field NMR methods. The obtained aerogels were characterized by a lower bulk density of 0.18-0.59 g/cm3 and 5.4-6.6 times higher oil-binding capacity compared to native potato starch. The chemical modification of starch helped to further alter the functional properties of the obtained aerogels, making them more effective oil binders, emulsifiers, and stabilizers (increasing the stability from 55 to 90%), which was especially evident for E 1450 preparation. Amylose content improved the aerogel properties, as waxy preparations were characterized by worse functional properties with the only exception of improved thickening ability. The most beneficial properties for the preparation of emulsions were observed for the aerogel obtained based on E 1450 normal potato starch.

Sulphated and carboxymethylated polysaccharides from Lycium barbarum L. leaves suppress the gelatinisation, retrogradation and digestibility of potato starch

SummaryProcessing starch with polysaccharides promotes broader applications, and it is important to consider all possible structural interactions among the molecular ingredients. In this study, polysaccharides isolated from Lycium barbarum L. were chemically modified by sulphation (SLP) and carboxymethylation (CLP), and the effects on the gelatinisation, retrogradation, and digestibility properties of the potato starch were investigated. After chemical modification, the slope of the curve between the molar mass and molecular radius of polysaccharides decreased, revealing that the relatively rigid rod‐like structure of native polysaccharide (0.78) changed to SLP's random linear chain (0.53) and CLP's spherical conformation (0.23), and the water solubility increased from 63.6% to 70.4% (SLP) and 84.1% (CLP) in distilled water at 25 °C. Moreover, testing polysaccharides as an additive indicated that both derivatives significantly decreased the peak viscosity, breakdown, and setback viscosity during potato starch pasting. Furthermore, the possible interactions could be further examined by structure analyses, suggesting the polysaccharide and SLP hydrogen bonds interacted with the surfaces of starch granules, while CLP penetrated the interiors of pasted starch granules, destroyed the helixes and backbone structure of starch, and formed type V crystallinity with amylose. The results of in vitro digestion analysis revealed that polysaccharides could decrease the amount of resistant starch and increase the amount of slowly digestible starch, which might be related to the inhibition of starch gelatinisation. These results facilitate the application of chemical derivatives in starch‐based functional foods and understand the interaction mechanism between potato starch and polysaccharides.

Studies on rheological behavior of native and octenyl succinic anhydride modified buckwheat (Fagopyrum esculentum) starch gel and improved flow properties thereof

AbstractThe effect of octenyl succinic anhydride (OSA) esterification (1%–3% of dry starch) on powder flowability, functionality, pasting, and gel rheological properties of buckwheat starch (BS) was examined. The physical and flow properties like bulk density, tapped density, Hausner's ratio, Carr's index, cohesion index, and caking strength of starch improved significantly after esterification, indicating better flow behavior and storage stability. The solubility and swelling power were significantly increased, leading to a significant increase in peak viscosity and breakdown, while pasting temperature, setback, and final viscosity decreased. The esterification also restricted the retrogradation, which appeared as a lower hardness of retrograded gel. Starch gel prepared at 5%, 10%, and 15% concentrations showed shear thinning behavior. The dynamic shear properties obtained within the linear viscoelastic region showed weak gel behavior with a more elastic nature and were also demonstrated by the Power law.Practical applicationThe OSA modifies the structure and surface of starch granules to change the physical properties which help in better powder flowability of the dried starch. The findings on powder flowability of BS may help powder manufacturers to control and design powder handling and transportation equipment by preparing non‐cohesive, non‐sticky, and flowable dried starch powder. The findings of the rheological characteristics of BS gels can be exploited in the preparation and optimization of soup premix, thickening agents, food additives, and gravies. Moreover, the OSA modified gel has a low retrogradation rate, which could be used in a product like bread and cakes.

The interaction of pectin with wheat starch and its influence on gelatinization and rheology

Adsorption isotherms have been determined for pectin obtained from citrus peel, apple pomace and creeping fig seeds onto wheat starch granules at room temperature. The results show that the amount adsorbed was significantly higher than can be accommodated solely on the surface of the granules and is, therefore, evidence that the pectin molecules must penetrate into the interior of the granules through the surface pores. The amount of pectin adsorbing/absorbing into the granules was found to decrease with increasing pectin molar mass. The adsorption/interpenetration of the pectin molecules reduced the amount of starch molecules leaching from the starch granules at room temperature. The transition temperatures and enthalpy of the gelatinization process were determined by differential scanning calorimetry and found to slightly increase in the presence of the pectin samples and with the degree of pectin interpenetration. The viscosity of starch – pectin dispersions on heating and cooling under constant shear increased with increasing pectin concentration. The final viscosity values of the starch pastes decreased with increasing pectin molar mass. The study provides a clearer understanding of the nature of starch–pectin interactions which will be of significant importance in food formulation.

Enhancement of starch-hydrocolloid synergism via the construction of an interchain entanglement

The effect of an interchain entanglement on the enhancement of starch and hydrocolloid synergism and their changes in structural and functional properties was investigated. Potato starch (PS) and xanthan gum (XG) were taken as research materials, and critical melting at the onset melting temperature of starch combined with freeze-thawing (CMFT) was used to promote interchain entanglement of PS/XG. The work suggested that CMFT effectively stabilized the aqueous mixture system of PS/XG, showing no phase separation in the water phase system, even after 12 h of storage. The PS/XG composite prepared by CMFT had significantly improved stability against acid, salt, and shear stress during the thermal process DSC demonstrated that CMFT raised the To of composite to ∼67 from 62.79 °C for native PS. RVA suggested that when pasted in distilled water, acid, and salt solution media, the breakdown viscosity of PS/XG composite declined to ∼380, 660, and 240 from 3615, 1541, and 1114 mPa s for native PS, respectively. CMFT significantly affected the apparent dropping properties of the starch paste, displaying a shorter and more cohesive paste drop against gravity. CMFT effectively improved the gelling properties of starch, with significant improvement in apparent surface adhesion properties and texture of gels, as well as freeze-thawing stability.

Physicochemical, Pasting, and Thermal Properties of Native Corn Starch-Mung Bean Protein Isolate Composites.

Starch is widely used in food and non-food industries because of its unique characteristics. However, native starch shows some weaknesses that restrict its applications. Recently, some studies have demonstrated the benefits of using protein to overcome these limitations. Therefore, the aim of the present study was to investigate the effect of mung bean protein isolate (MBPI) (2%, 4%, 6%, and 8%) on the physicochemical, pasting, and thermal properties of native corn starch (NCS), as a novel starch-protein composite. Higher swelling power (SP), water absorbance capacity (WAC), and solubility values of NCS were observed with increasing MBPI concentration. Additionally, by the addition of MBPI, the rapid visco analyzer (RVA) showed a reduction in pasting temperature (77.98 to 76.53 °C), final viscosity (5762 to 4875 cP), and setback (3063 to 2400 cP), while the peak viscosity (4691 to 5648 cP) and breakdown (1992 to 3173 cP) increased. The thermal properties of NCS/MBPI gels investigated by differential scanning calorimetry (DSC) showed higher onset, peak, and conclusion temperatures (69.69 to 72.21 °C, 73.45 to 76.72 °C, and 77.75 to 82.26 °C, respectively), but lower gelatinization enthalpy (10.85 to 8.79 J/g) by increasing MBPI concentration. Fourier transform infrared spectroscopy (FT-IR) indicated that the addition of MBPI decreased the amount of hydrogen bonds within starch. Furthermore, after three cycles of freeze-thaw shocks, the syneresis of NCS-MBPI composites decreased from 38.18 to 22.01%. These results indicated that the MBPI could improve the physicochemical properties of NCS, especially its syneresis and retrogradation characteristics.

Impact of Low‐Pressure Argon Plasma on Structural, Thermal, and Rheological Properties of Corypha umbraculifera L. Starch: A Non‐Conventional Source of Stem Pith Starch

AbstractThe impact of low‐pressure argon plasma on structural, pasting, rheological, and thermal characteristics of starch from talipot palm (Corypha umbraculifera L.) is analyzed. Talipot palm is a non‐conventional source of starch. The starch is subjected to a different plasma power with different time duration. Exposure of talipot starch to plasma with different power (7.5 and 15 W) and time (30 min and 1 h) significantly affects the physicochemical and functional properties. SEM micrographs show that the plasma treatment causes severe damage to the granule surface. The pasting profile of talipot starch is considerably decreased by plasma treatment. The relative crystallinity and thermal properties are also decreased by plasma treatment. Plasma‐treated talipot starches exhibit a decreased storage and loss modulus indicating weak gel formation. However, the treatment considerably increases the paste clarity of the starch gel.

Structure, in vitro starch digestibility and physicochemical properties of starch isolated from germinated Bambara groundnut.

This study investigated the effect of germination time (0, 24, 48 and 72h) on the physicochemical characteristics, in vitro starch digestibility and microstructural changes in Bambara groundnut starch. The starch yield, lightness (L*) value, amylose content and resistant starch contents of isolated starches decreased significantly (p ≤ 0.05) with increasing germination time. Scanning electron microscopy revealed that starch from raw and germinated Bambara grains were smooth with no evidence of starch degradation and were mainly oval shaped, with some granule's irregular and kidney shaped. Water absorption capacity (1.33-1.90g/g), swelling power (2.12-16.53g/g), solubility index (1.14-13.04g/g), and dispersibility (75.92-86.47%) greatly increased as germination timed increased. Germination did not alter the X-ray diffraction pattern (Type-A) but increased the relative crystallinity of the starches. The peak gelatinization temperatures (73.23-73.91°C) of starch from germinated Bambara were significantly higher than native starch (72.81°C). Native starch and starch from germinated Bambara grains had substantially high proportion of resistant starch (approx. 73%) and high pasting temperatures (approx. 88°C). Conclusively, germination significantly changed starch structure at molecular level and impacted functionality.

Physicochemical properties of a new starch from turion of Spirodela polyrhiza

Starch from the turion of Spirodela polyrhiza ZH0196 is a new resource with great development values. The solubility, swelling power, thermogravimetric analysis (TGA), enzyme susceptibility, and in vitro digestibility of Spirodela starch, paste clarity, settling volume, and the rheology property of Spirodela starch paste, strength, elasticity, and freeze-thaw stability of Spirodela starch gel were studied. The properties of Spirodela starch were compared with those of normal corn starch and rice starch. The possible relationship between properties and structure of three kinds of starch was analyzed by principal component analysis (PCA). The results showed that the rapidly digested starch, slowly digested starch and resistant starch were 86.63 %, 4.43 %, and 8.94 % respectively. Spirodela starch has higher strength (17.77 g), elasticity (4.98 g/s) of starch paste, and freeze-thaw stability of starch gel than those of normal corn starch and rice starch. The rheological results showed that the stickiness maintainability of starch paste was normal corn >Spirodela > rice and Spirodela starch paste had compact paste structures with the tan δ between 0.33 and 0.38. This study has significant value in the application of Spirodela starch.

Natural Variation of Fatty Acid Desaturase Gene Affects Linolenic Acid Content and Starch Pasting Viscosity in Rice Grains.

Rice, as one of the main food crops, provides a vital source of dietary energy for over half the world’s population. The OsFAD3 gene encodes fatty acid desaturase, catalyzing the conversion of linoleic acid (LA) to alpha-linolenic acid (ALA) in rice. However, the genetic characterization of OsFAD3 and its role in the conversion of LA to ALA remains elusive. Here, we validated the effects of two homologous genes, OsFAD3-1 and OsFAD3-2, on the ALA and LA/ALA ratio in rice grains using near-isogenic lines. Two major haplotypes of OsFAD3-1 are identified with different effects on the ALA and LA/ALA ratio in rice germplasm. High expression of OsFAD3-1 is associated with high ALA accumulation and eating quality of rice grains. Overexpression of OsFAD3-1 driven by a seed-specific promoter increases the ALA content up to 16-fold in the endosperm. A diagnostic marker is designed based on an 8-bp insertion/deletion in the OsFAD3-1 promoter, which can recognize OsFAD3-1 alleles in rice. These results indicate that OsFAD3-1 is a useful target gene in marker-assisted breeding programs to improve varieties with high ALA and appropriate LA/ALA ratio in brown rice.