Starch-based Feedstocks Research Articles

Fermentative valorisation of xylose-rich hemicellulosic hydrolysates from agricultural waste residues for lactic acid production under non-sterile conditions

Lactic acid (LA) is a platform chemical with diverse industrial applications. Presently, commercial production of LA is dominated by microbial fermentation using sugary or starch-based feedstocks. Research pursuits emphasizing towards sustainable production of LA using non-edible and renewable feedstocks have accelerated the use of lignocellulosic biomass (LCB). The present study focuses on the valorisation of xylose derived from sugarcane bagasse (SCB) and olive pits (OP) through hydrothermal and dilute acid pretreatment, respectively. The xylose-rich hydrolysate obtained was used for LA production by homo-fermentative and thermophilic Bacillus coagulans DSM2314 strain under non-sterile conditions. The fed-batch mode of fermentation resulted in maximum LA titers of 97.8, 52.4 and 61.3 g/L with a yield of 0.77, 0.66 and 0.71 g/g using pure xylose, xylose-rich SCB and OP hydrolysates, respectively. Further, a two-step aqueous two-phase system (ATPS) extraction technique was employed for the separation and recovery of LA accumulated on pure and crude xylose. The LA recovery was 45 – 65% in the first step and enhanced to 80–90% in the second step.The study demonstrated an efficient integrated biorefinery approach to valorising the xylose-rich stream for cost-effective LA production and recovery.

Integrating 1G with 2G Bioethanol Production by Using Distillers’ Dried Grains with Solubles (DDGS) as the Feedstock for Lignocellulolytic Enzyme Production

First-generation (1G) bioethanol is one of the most used liquid biofuels in the transport industry. It is generated by using sugar- or starch-based feedstocks, while second-generation (2G) bioethanol is generated by using lignocellulosic feedstocks. Distillers’ dried grains with solubles (DDGS) is a byproduct of first-generation bioethanol production with a current annual production of 22.6 million tons in the USA. DDGS is rich in fiber and valuable nutrients contents, which can be used to produce lignocellulolytic enzymes such as cellulases and hemicellulases for 2G bioethanol production. However, DDGS needs a pretreatment method such as dilute acid, ammonia soaking, or steam hydrolysis to release monosaccharides and short-length oligosaccharides as fermentable sugars for use in microbial media. These fermentable sugars can then induce microbial growth and enzyme production compared to only glucose or xylose in the media. In addition, selection of one or more suitable microbial strains, which work best with the DDGS for enzyme production, is also needed. Media optimization and fermentation process optimization strategies can then be applied to find the optimum conditions for the production of cellulases and hemicellulases needed for 2G bioethanol production. Therefore, in this review, a summary of all such techniques is compiled with a special focus on recent findings obtained in previous pieces of research conducted by the authors and by others in the literature. Furthermore, a comparison of such techniques applied to other feedstocks and process improvement strategies is also provided. Overall, dilute acid pretreatment is proven to be better than other pretreatment methods, and fermentation optimization strategies can enhance enzyme production by considerable folds with a suitable feedstock such as DDGS. Future studies can be further enhanced by the technoeconomic viability of DDGS as the on-site enzyme feedstock for the manufacture of second-generation bioethanol (2G) in first-generation (1G) ethanol plants, thus bridging the two processes for the efficient production of bioethanol using corn or other starch-based lignocellulosic plants.

Trends of Progress in Setting up Biorefineries in Developing Countries: A Review of Bioethanol Exploration in Nigeria

In recent times, limitations and adverse effects of fossil fuels have significantly attracted researchers' attention to green fuels worldwide, especially in developed nations. As a way of assessing this actualization of biorefineries establishment in developing nations, this report surveys the works done by various researches towards this great course in terms of promoting and gaining the attention of both government and private investors about the technical and economic feasibility of embracing the use of biofuels, a case of bioethanol in Nigeria. Different classes of feedstocks were reviewed for the laboratory-scale, process scale-up, pilot plant, and techno-economic studies regarding ascertaining the technical and economic feasibility of local setup of a functional biorefinery in Nigeria, which would be beneficial environmentally and economically. The literature survey unveiled that the Bioethanol yield obtained from sugarcane-juice (72.7 %), banana-stems (84.0 %), and cassava (92.0 %) were found to be of highest potential amongst other sugar-based, lignocellulosic, and starch-based feedstock, respectively. The survey further unveils that the volume of process scale-up and economic feasibility studies does not correlate well with the laboratory-scale studies. The bulk of the research works on bioethanol has given preferential attention to laboratory studies. Only a few studies have looked into the commercialization (i.e., scale-up) of the laboratory findings and the economic implications. Presently, only sugarcane and a few cassavas are reported in the literature so far. It is, therefore, necessary for further studies to give attention to the investigation of the commercializing locally developed technologies and the exploration of their economic benefits.

Land use mediated GHG emissions and spillovers from increased consumption of bioplastics

Bioplastic production is a small but fast growing sector in the global bioeconomy, which may benefit from public support measures in the future as governments seek to promote more sustainable consumption patterns. Here we assess the potential net economy-wide impacts of a 5% bioplastic target relative to current plastic consumption in the main producing regions. We compare two alternative policy strategies to achieve the target in a general equilibrium framework that allows for substitution between conventional and bio-based plastics: a subsidy on bioplastics versus a tax on fossil-based plastic consumption. Our study is the first to quantify global greenhouse gas (GHG) emissions from an increased demand for bioplastics on a global scale, produced from arable crops, considering both direct and indirect land use change (LUC). The tax provokes a contraction of all sectors that employ plastics, which leads to a drop of 0.07% in global real GDP, whereas the subsidy has no significant effect on the global economy. Both tax and subsidy reduce world demand for petroleum products, by 0.37% and 0.07%, respectively, boosting demand for sugar- and starch-based feedstock in the bioplastic industry. This leads to emissions from LUC globally, which correspond to a carbon payback time of 22 years on average, with the associated annual abatement costs of over US$2000 per tonne of CO2-eq. The tax has greater GHG reduction potential in bioplastic producing regions but generates greater economic and environmental spillover effects in countries that do not enforce the target. Results show that promoting bioplastic consumption is not a cost-effective strategy for climate change mitigation if based on conventional feedstock, due to market-mediated GHG emissions from LUC. Bioplastics are not necessarily more sustainable than conventional polymers just because they are bio-based, although further assessment of potential environmental gains associated with biodegradability and recyclability is desirable.

Land use mediated GHG emissions and spillovers from increased bioplastic consumption

Bioplastic production is a small but fast growing sector in the global bioeconomy that may benefit from future public support measures as governments seek to promote more sustainable consumption patterns. Here we assess the potential net economy-wide impacts of a 5% bioplastic target relative to current plastic consumption in leading producing regions. We compare two alternative policy strategies to achieve the target in a general equilibrium framework that allows for substitution between conventional and bio-based plastics: a subsidy on bioplastics vs. a tax on fossil-based plastic consumption. Our study is the first to quantify global greenhouse gas (GHG) emissions from an increased demand for bioplastics produced from conventional crops, considering both direct and indirect land use change (LUC). The tax generates a contraction of all sectors that employ plastics and hence to a 0.07% drop in global real GDP, whereas the subsidy has no significant effect on the global economy. Both policy scenarios lead to a reduction in the worldwide demand for petroleum products, by 0.08% and 0.40% respectively, although a substantial increase in the demand for sugar- and starch-based feedstock is observed, driven by the bioplastic industry. Due to direct and indirect LUC that occur at global scale, GHG emissions increase rather than decrease, by 0.96% with the subsidy and 5.01% with the tax. Trade mediated effects also generate land and GHG spillovers in countries that do not enforce the target. Results show that promoting bioplastic consumption is not effective for climate change mitigation if based on conventional biomass feedstock when emissions from LUC are taken into account, although it can contribute to oil security in the main plastic producing regions. A biomass resource base does not guarantee the sustainability of bioplastics vis-a-vis fossil-based polymers, although further assessment of potential environmental gains associated with biodegradability and recyclability is desirable.

Enzymatic conversion of sweet potato granular starch into fermentable sugars: Feasibility of sweet potato peel as alternative substrate for α-amylase production

Abstract Currently, the conventional process for bioethanol production from starch-based feedstocks incurs high costs. Alternatively, the granular starch hydrolysis process makes starch gelatinization unnecessary and reduces energy requirement, thereby conferring economic advantages. The aim of this study was to establish an effective approach for enzymatic conversion of sweet potato granular starch into fermentable sugars. The effects of some factors on α-amylase production by A. niger were evaluated using a Central Composite Rotational Design (CCRD) combined with Response Surface Methodology (RSM). An improved yield of 214.28 U mL−1 was achieved after 6.5 days of cultivation, at pH 6.0, using 2.0% sweet potato peel as substrate. The α-amylases produced were more active at 60 °C at pH 4.5. Hydrolysis of sweet potato granular starch was also optimized by RSM and CCRD, using A. niger crude extract. It proceeded optimally at pH 4.0 at 50 °C, with an α-amylase loading value of 31.25 U g−1 dry feedstock and a reaction time of 37 h. Under these conditions, a saccharification rate of 78.33% was obtained. This work reports, for the first time, the production of remarkable yields of α-amylase through the use of sweet potato peel. In addition, it describes the achievement of expressive levels of glucose from sweet potato granular starch hydrolysis. These results open up new possibilities for rendering bioethanol production from sweet potato economically competitive.

Biobutanol from cheese whey.

At present, due to environmental and economic concerns, it is urgent to evolve efficient, clean and secure systems for the production of advanced biofuels from sustainable cheap sources. Biobutanol has proved better characteristics than the more widely used bioethanol, however the main disadvantage of biobutanol is that it is produced in low yield and titer by ABE (acetone-butanol-ethanol) fermentation, this process being not competitive from the economic point of view. In this review we summarize the natural metabolic pathways for biobutanol production by Clostridia and yeasts, together with the metabolic engineering efforts performed up to date with the aim of either enhancing the yield of the natural producer Clostridia or transferring the butanol production ability to other hosts with better attributes for industrial use and facilities for genetic manipulation. Molasses and starch-based feedstocks are main sources for biobutanol production at industrial scale hitherto. We also review herewith (and for the first time up to our knowledge) the research performed for the use of whey, the subproduct of cheese making, as another sustainable source for biobutanol production. This represents a promising alternative that still needs further research. The use of an abundant waste material like cheese whey, that would otherwise be considered an environmental pollutant, for biobutanol production, makes economy of the process more profitable.

Consolidated bioprocessing strategy for ethanol production from Jerusalem artichoke tubers by Kluyveromyces marxianus under high gravity conditions

Developing an innovative process for ethanol fermentation from Jerusalem artichoke tubers under very high gravity (VHG) conditions. A consolidated bioprocessing (CBP) strategy that integrated inulinase production, saccharification of inulin contained in Jerusalem artichoke tubers and ethanol production from sugars released from inulin by the enzyme was developed with the inulinase-producing yeast Kluyveromyces marxianus Y179 and fed-batch operation. The impact of inoculum age, aeration, the supplementation of pectinase and nutrients on the ethanol fermentation performance of the CBP system was studied. Although inulinase activities increased with the extension of the seed incubation time, its contribution to ethanol production was negligible because vigorously growing yeast cells harvested earlier carried out ethanol fermentation more efficiently. Thus, the overnight incubation that has been practised in ethanol production from starch-based feedstocks is recommended. Aeration facilitated the fermentation process, but compromised ethanol yield because of the negative Crabtree effect of the species, and increases the risk of contamination under industrial conditions. Therefore, nonaeration conditions are preferred for the CBP system. Pectinase supplementation reduced viscosity of the fermentation broth and improved ethanol production performance, particularly under high gravity conditions, but the enzyme cost should be carefully balanced. Medium optimization was performed, and ethanol concentration as high as 94·2 g l(-1) was achieved when 0·15 g l(-1) K(2) HPO(4) was supplemented, which presents a significant progress in ethanol production from Jerusalem artichoke tubers. A CBP system using K. marxianus is suitable for efficient ethanol production from Jerusalem artichoke tubers under VHG conditions. Jerusalem artichoke tubers are an alternative to grain-based feedstocks for ethanol production. The high ethanol concentration achieved using K. marxianus with the CBP system not only saves energy consumption for ethanol distillation, but also significantly reduces the amount of waste distillage discharged from the distillation system.

Production of ethanol from winter barley by the EDGE (enhanced dry grind enzymatic) process

BackgroundUS legislation requires the use of advanced biofuels to be made from non-food feedstocks. However, commercialization of lignocellulosic ethanol technology is more complex than expected and is therefore running behind schedule. This is creating a demand for non-food, but more easily converted, starch-based feedstocks other than corn that can fill the gap until the second generation technologies are commercially viable. Winter barley is such a feedstock but its mash has very high viscosity due to its high content of β-glucans. This fact, along with a lower starch content than corn, makes ethanol production at the commercial scale a real challenge.ResultsA new fermentation process for ethanol production from Thoroughbred, a winter barley variety with a high starch content, was developed. The new process was designated the EDGE (enhanced dry grind enzymatic) process. In this process, in addition to the normal starch-converting enzymes, two accessory enzymes were used to solve the β-glucan problem. First, β-glucanases were used to hydrolyze the β-glucans to oligomeric fractions, thus significantly reducing the viscosity to allow good mixing for the distribution of the yeast and nutrients. Next, β-glucosidase was used to complete the β-glucan hydrolysis and to generate glucose, which was subsequently fermented in order to produce additional ethanol. While β-glucanases have been previously used to improve barley ethanol production by lowering viscosity, this is the first full report on the benefits of adding β-glucosidases to increase the ethanol yield.ConclusionsIn the EDGE process, 30% of total dry solids could be used to produce 15% v/v ethanol. Under optimum conditions an ethanol yield of 402 L/MT (dry basis) or 2.17 gallons/53 lb bushel of barley with 15% moisture was achieved. The distillers dried grains with solubles (DDGS) co-product had extremely low β-glucan (below 0.2%) making it suitable for use in both ruminant and mono-gastric animal feeds.

Utilization and biodegradation of starch stillage (distillery wastewater)

Stillage (distillery wastewater) is the main by-product originating in distilleries, and its volume is approximately 10 times that of ethanol produced. It is not surprising that the utilization of the stillage raises serious problems, and that many attempts have been made all over the world to solve them. In Poland most of the ethanol (about 90%) is produced from starch-based feedstocks, i.e. grains and potatoes. Starch feedstocks are widely used for spirit production also in other European countries, as well as outside Europe. The manuscript provides an overview of global fuel ethanol production and information on methods used for starch-based stillage biodegradation and utilization. The methods presented in this paper have been classified into two major groups. One of these includes the mode of utilizing starch stillage, the other one comprises methods, both aerobic and anaerobic, by which the stillage can be biodegraded.

Progress in the production of bioethanol on starch-based feedstocks

Bioethanol produced from renewable biomass, such as sugar, starch, or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally friendly. Although, the priority in global future ethanol production is put on lignocellulosic processing, which is considered as one of the most promising second-generation biofuel technologies, the utilization of lignocellulosic material for fuel ethanol is still under improvement. Sugar-based (molasses, sugar cane, sugar beet) and starch-based (corn, wheat, triticale, potato, rice, etc.) feedstock are still currently predominant at the industrial level and they are, so far, economically favorable compared to lingocelluloses. Currently, approx. 80 % of total world ethanol production is obtained from the fermentation of simple sugars by yeast. In Serbia, one of the most suitable and available agricultural raw material for the industrial ethanol production are cereals such as corn, wheat and triticale. In addition, surpluses of this feedstock are being produced in our country constantly. In this paper, a brief review of the state of the art in bioethanol production and biomass availability is given, pointing out the progress possibilities on starch-based production. The progress possibilities are discussed in the domain of feedstock choice and pretreatment, optimization of fermentation, process integration and utilization of the process byproducts.

Screening Extremophiles for Bioconversion Potentials

A screening protocol was developed and implemented to evaluate extremophiles, in particular hyperthermophiles and thermoacidophiles, for their capacity to transform starch-based feedstocks to high-value organic acids and solvents. Screening results of 14 extremophiles showed promising growth and biotransformation potentials. In particular, Hyperthermus butylicus, Thermococcus litoralis, and Sulfolobus acidocaldarius were identified as producers of both organic acids and solvents under the screening protocol. The screening effort presented here represents an important step toward realization of biotransformation potentials of extremophiles, potentially improving upon biomass-based processes.