Standardized Ileal Digestibility Of Met Research Articles

Comparison of Amino Acid Digestibility between Commercial Crossbred Pigs and Mini-Jeju Island Native Pigs.

The objectives of this study were to determine the apparent ileal digestibility and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in feed ingredients, compare the ileal digestibility of CP and AA between commercial crossbred pigs and mini-Jeju Island native pigs (JINP), and develop models for estimating SID of CP and AA for commercial pigs using mini-JINP data. The study involved five crossbred commercial pigs (31.5 ± 1.6 kg of body weight and 11 weeks of age; Landrace × Yorkshire) and five mini-JINP (31.0 ± 3.2 kg body weight and 20 weeks of age). The pigs were surgically equipped with a T-cannula at the end of ileum. Each pig breed was assigned to 5 dietary treatments in a 5 × 10 incomplete Latin square design with 10 periods. Four experimental diets were formulated to contain each of soybean meal, corn gluten feed, copra meal, and sesame expellers as the sole source of nitrogen. A nitrogen-free diet was also prepared to determine basal endogenous losses of CP and AA. No interaction between breed and feed ingredient was observed for the digestibility of CP and all indispensable AA. The SID of CP and all indispensable AA, except Arg, His, and Lys, did not differ between the two breeds of pigs. Prediction equations were developed for SID of CP and AA of commercial pigs using the SID values of mini-JINP: SID of CP (%) = (1.02 × SID of CP in mini-JINP) - 5.20 with r2 = 0.97 and p < 0.05; SID of Lys (%) = (1.12 × SID of Lys in mini-JINP) - 9.10 with r2 = 0.98 and p < 0.05; and SID of Met (%) = (1.08 × SID of Met in mini-JINP) - 4.27 with r2 = 0.96 and p < 0.05. The digestibility for most AA in feedstuffs for commercial pigs can be estimated using data from mini-JINP.

PSVI-28 Interactive effects of different fiber and lipid sources on amino acid digestibility in growing pigs

Abstract A study was conducted to determine the effects of different fiber and lipid sources on the digestibility of amino acids (AA) in growing pigs. Five diets were fed in this study. The diets were corn-soybean meal-based diet with either soybean hulls (SBH) or sugar beet pulp (SBP) as fiber source and either soybean oil (SBO) or choice white grease (CWG) as lipid source in a 2 × 2 factorial arrangement; and a N-free diet. The diets were formulated to be similar in content of AA, calcium, available phosphorus, and total dietary fiber. The SBH and SBP were each included in their respective diets at 15%, whereas the SBO and CWG were each included in their respective diets at 4.5%. The 5 diets were fed to 10 ileal-cannulated pigs [5 barrows and 5 gilts; initial average body weight (BW) = 54.8 ± 5.6 kg)]in a 5 × 5 Latin square design to give 10 replicates per diet. On an as fed basis, SBH and SBP contained 11.3 and 8.8% crude protein, 66.1 and 49.8% total non-starch-polysaccharides (NSP), 54.3 and 29.5% insoluble NSP, and 11.8 and 20.3% soluble NSP, respectively. The SBO and CWG contained &amp;gt;98.5 and &amp;gt;98.5% crude fat, 14.7 and 35.6% saturated fatty acids, and 76.6 and 62.5% unsaturated fatty acids, respectively. Dietary fiber source and lipid sources did not interact on standardized ileal digestibility (SID) of AA. The SBH- and SBP-containing diets did not differ regarding SID of all AA except Met and Trp. Pigs fed SBP-containing diets had lower (P &amp;lt; 0.05) SID of Met and greater (P &amp;lt; 0.05) SID of Trp than those fed SBH-containing diets by 2.5 and 4.7%, respectively. The SBO- and CWG-containing diets did not differ regarding SID of all AA except Trp. The SID of Trp for SBO-containing diets was greater (P &amp;lt; 0.05) than that for CWG-containing diets by 2.6%. In conclusion, the lack of interaction between dietary fiber and lipid sources on the SID of AA implies that these two factors (fiber and lipid sources) may exert their effects on ileal digestibility of AA independently if the fiber and lipid sources are included in diets for growing pigs at same levels included in the diets fed in the current study. The dietary fiber source and dietary lipid source had limited effects on the digestibility of most AA, implying that replacement of one fiber source with one another fiber source or of one lipid source with another lipid source in diets for growing pigs may have limited effect on digestibility of AA if the fiber and lipid sources are included in diets for growing pigs at same levels included in the diets fed in the current study.

Evaluation of exogenous phytase in high-phytate diets for broiler chickens and pigs

There is a gap in the understanding of the relationship between dietary phytate levels and the relative efficacy of phytase to improve amino acid (AA) digestibility in pigs and chickens. Two experiments were conducted to investigate the effect of exogenous phytase on standardized ileal digestibility (SID) of AA and the apparent ileal digestibility (AID) of P in both standard- (SP) and high-phytate (HP) diets for broilers and swine. There were either 40 cages of Cobb 500 male broilers or 10 crossbred barrows (35 kg) fitted with ileal T-cannulas. Both studies were allotted to five dietary treatments (8 replicates). Treatments consisted of four corn-soybean meal-based diets arranged in a 2 × 2 factorial of standard or high phytate and exogenous phytase at 0 or 1 000 phytase units (FYT)/kg; and one N-free diet. Birds were fed a common starter diet from d 0 to 20 and fed experimental diets from d 20 to 25. Birds were euthanized on d 25 via CO2 asphyxiation, and digesta were collected from the terminal ileum. Pigs were fed for a total of four 7-d periods, where digesta were collected on d 6 and 7 of each period. Diet and digesta samples were analyzed for DM, N, Ti, AA, and P to determine AA and P digestibility. The SID of AA was determined by correcting the AID of AA for the basal endogenous losses estimated using the N-free diet. Main effects of the diet type (standard or HP) and phytase (0 or 1 000 FYT/kg), and the interaction of diet type and phytase were evaluated. For both experiments, the HP diets produced lower SID of AA compared to the SP (P < 0.001). For broilers, there was a phytase effect (P < 0.001) for the SID of all AAs evaluated regardless of the diet type. For pigs, phytase improved (P < 0.05) the SID of Met, Lys, Cys, Glu and Ser and tended to improve (P < 0.10) Arg, Leu, Thr, and Tyr. There were no significant interactions for either experiment. For both experiments, AID of P was lower for the HP diets (P < 0.01), and phytase produced greater AID of P for both diet types (P < 0.01). These data indicate that phytase greatly improves the digestibility of P for broilers and pigs and has the ability to significantly increase the digestibility of amino acids for these animals, regardless of the dietary phytate P.

153 Effect of Exogenous Phytase on the Digestibility of Amino Acids and Phosphorus in Standard and High Phytin Swine Diets

Abstract The objective of the following study was to investigate the effect of exogenous phytase (Hiphorius 40, DSM Nutritional Products) on the standardized ileal digestibility (SID) of amino acids (AA) and the apparent ileal digestibility (AID) of P in both standard and high phytin (HP) swine diets. The study consisted of 4 diets arranged in a 2 x 2 factorial of standard or high phytin and exogenous phytase at 0 or 1,000 FYT/kg; and one N-free diet. Ten crossbred male pigs fitted with ileal T-cannulas were allotted to the 5 dietary treatments in duplicate 5 x 4 incomplete Latin-square design. Pigs were fed for a total of four 7-d periods, where digesta was collected on d 6 and 7 of each period. Diet and digesta samples were analyzed for dry matter, N, Ti, AA, and P to determine AA and P digestibility. The SID of AA was determined by correcting the AID of AA for the basal endogenous losses estimated using the N-free diet. Data were subjected to an ANOVA where main effects of the diet type (standard vs. HP) and phytase were evaluated as well as the interaction of diet type and phytase. If the ANOVA was significant the means were separated by Tukey’s method. The basal endogenous AA losses of pigs in this study ranged from 0.115 (Met) to 0.921 (Thr) g/kg DM and 0.308 (Cys) to 9.455 (Pro) g/kg DM for indispensable and dispensable AA, respectively. The HP diets produced less SID of all AA compared with the standard diets (P &amp;lt; 0.001) with an average SID reduction of 6.5% for indispensable AA. Phytase improved (P &amp;lt; 0.05) the SID of Met, Lys, Arg, Cys, Glu and Ser and tended to improve (P &amp;lt; 0.10) Leu, Thr, and Tyr. There were no significant diet type*phytase interactions. The AID of P was less (P &amp;lt; 0.01) for the HP diets relative to the standard (39.12% vs. 45.84%), and phytase produced greater (P &amp;lt; 0.01) AID of P for both diet types (25.62% vs. 59.34%). These data indicate that phytase can improve the digestibility of many AA and P in both standard and HP diets for growing pigs.

169 Standardized ileal digestible amino acids and net energy contents in full fat and defatted black soldier fly larvae meals (Hermatia illucens) fed to growing pigs

Abstract Two experiments were conducted to determine the standardized ileal digestibility (SID) of AA (Exp. 1) and available energy (Exp. 2) in two black soldier fly larvae meal (BSFLM) sources [full fat (FF) and defatted (DF)] for growing pigs. Two corn starch-based diets were formulated with FF and DF BSFLM as the sole sources of AA. A nitrogen-free diet was also used and the corn starch:sucrose:oil ratio was kept constant among diets to calculate digestible energy. In Exp. 1, 8 ileal-cannulated barrows (25.1±0.41 kg initial BW) were used in a replicated 2 x 2 Latin square design (n = 8). In each period, pigs were adjusted to diets for 5d followed by 2d of continuous ileal digesta collection for 8 h. The SID of AA were calculated using basal endogenous losses for pigs fed a nitrogen-free diet. In Exp. 2, 8 barrows (23.4±0.54 kg initial BW) were used in a partially replicated Latin square design (n=8). In each period, pigs were adjusted to diets for 7d, followed by 5d of total urine and fecal grab sample collection, and 24hr in indirect calorimetry chambers to determine heat production. The SID of Arg, Val, Ala, and Pro tended to be less, and the SID of Met tended to be greater for the FF versus the DF BSFLM (P=0.034, 0.090, 0.053, 0.065, 0.074, respectively). Digestible, metabolizable, and net energy (4101 vs 3257±224 kcal/kg) were greater for the FF versus the DF BSFLM (P&amp;lt; 0.05). The ATTD of phosphorus, neutral detergent fiber, and acid detergent fiber were greater for the FF versus the DF BSFLM (P&amp;lt; 0.05). In conclusion, the SID AA in FF and DF BSFLM were high and similar to those of soybean meal. The FF BSFLM is a better source of available energy for growing pigs compared to DF BSFLM.

PSIV-24 Comparison of amino acid digestibility in feed ingredients for mini and commercial pigs

Abstract The objectives were to determine the apparent ileal digestibility and standardized ileal digestibility (SID) of CP and AA in feed ingredients and to develop prediction equations for estimating SID of feed ingredient for commercial pigs using data from mini pigs. Six 11-week-old crossbred commercial pigs (31.5 ± 1.6 kg of BW; Landrace × Yorkshire) and six 20-week-old mini pigs (31.0 ± 3.2 kg BW; Jeju Island’s native pig) were individually housed in pens and all pigs were surgically fitted with a T-cannula at the distal ileum. Each pig breed was assigned to 5 dietary treatments in a 5 × 10 incomplete Latin square design involving 10 periods, respectively. Four diets were prepared to contain soybean meal, corn gluten feed, copra meal, and sesame expellers as the sole source of N. An N-free diet was formulated to estimate basal ileal endogenous losses of CP and AA. There were no interactions between breed and feed ingredient for the digestibility of CP and AA. The digestibility of CP and AA, except Arg, His, Val, and Pro, were not different between mini pigs and commercial pigs. Prediction equations for SID CP and AA of commercial pigs using SID value of mini pigs were: SID of CP (%) = (1.02 × SID of CP in mini pigs) – 5.20 with r2 = 0.97 and P &lt; 0.05; SID of Lys (%) = (1.12 × SID of Lys in mini pigs) – 9.10 with r2 = 0.98 and P &lt; 0.05; and SID of Met (%) = (1.08 × SID of Met in mini pigs) – 4.27 with r2 = 0.96 and P &lt; 0.05. In conclusion, Jeju Island’s native mini pigs can be used for estimating AA digestibility in feed ingredients for commercial pigs.

Effect of keratinase on ileal amino acid digestibility in five feedstuffs fed to growing pigs

ObjectiveThis study was conducted to evaluate the effect of keratinase (KE) on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in rice bran, cottonseed meal (CSM), rapeseed meal (RSM), corn distillers dried grains with solubles (DDGS), and peanut meal (PNM).MethodsTwelve crossbred barrows (Duroc×Landrace×Yorkshire, 50.5±1.4 kg body weight [BW]) fitted with T-cannulas at the terminal ileum were allotted to a 12×6 Youden Square design with 12 diets and 6 periods. The treatment diets included rice bran, CSM, RSM, corn DDGS, PNM, or corn-soybean meal (cSBM) supplemented with 0.05% KE or not. Diets were given to pigs at a level of 3% BW in two equal meals. The endogenous AA losses were the mean results of three previously experiments determined by a same nitrogen-free diet fed to pigs. Pigs had free access to water during the experiment.ResultsThe KE supplementation improved (p<0.05) the AID and SID of Met, Thr, Val, Asp, Cys, and Tyr in rice bran. Inclusion of KE increased (p<0.05) the AID and SID of Met and Val in CSM. The KE supplementation decreased (p<0.05) the AID and SID of His in RSM and all measured AA except for Arg, Met, Trp, Val, Gly, and Pro in corn DDGS. There was an increase (p<0.05) in AID and SID of Leu, Ile, Met, Ala, Cys, Ser, and Tyr in PNM supplemented with KE compared with that without KE. Inclusion of KE increased (p<0.05) the AID and SID of crude protein, Leu, Ile, Phe, Thr, Asp, and Ser in cSBM.ConclusionThis study indicated that KE had different effects on ileal AA digestibility of feedstuffs for growing pigs, which can give some usage directions of KE in swine feed containing those detected feedstuffs.

The ileal digestibility of most amino acids is greater in red dog than in wheat middlings when fed to growing pigs

The objective of this experiment was to determine the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of CP and AA in 10 sources of wheat middlings and in 1 source of red dog. Ten diets that each contained 1 of the 10 sources of wheat middlings and 1 diet that contained red dog as the only source of protein and AA were formulated. An N-free diet was used to determine basal endogenous losses of CP and AA. Chromic oxide (0.4%) was added to all diets as an indigestible marker. Twelve pigs (BW: 29.23 ± 1.5 kg) were fitted with a T-cannula in the distal ileum. Pigs were allotted to a 12 × 8 Youden square design with 12 diets and eight 7-d periods. The initial 5 d of each period was the adaptation period, but ileal digesta were collected on the last 2 d of each period. Results indicated that the AID of CP in wheat middlings was 31.1 ± 6.9%, but the AID of CP in red dog (47.0%) was greater ( < 0.05) than the average AID of CP for wheat middlings. The AID of indispensable AA in wheat middlings ranged from 30.1 ± 5.4% for Lys to 67.7 ± 2.2% for His, and the AID of indispensable AA in red dog ranged from 53.7% in Val to 86.2% for Met. The average SID of CP in wheat middlings was 61.5 ± 5.1%, and there were no differences among the 10 sources of wheat middlings, but the SID of CP in red dog (78.5%) was greater ( < 0.05) than in wheat middlings. The SID of Arg, His, and Asp in wheat middlings was 81.4 ± 2.7%, 77.7 ± 2.1%, and 66.4 ± 2.7%, respectively, and no differences among sources of wheat middlings were observed for these AA. The SID of Met (73.6 ± 1.8%) and the SID of Ala (54.8 ± 4.9%) tended ( = 0.071 and = 0.090, respectively) to be different among sources of wheat middlings, and the SID of all other AA was different ( < 0.05) among the 10 sources of wheat middlings. There were no differences between red dog and wheat middlings for the SID of Arg, His, and Ser, and the SID of Cys was less ( < 0.05) in red dog than in wheat middlings, but for all other AA, the SID in red dog was greater ( < 0.05) than in wheat middlings. In conclusion, wheat middlings and red dog contain approximately the same quantities of AA, but the AID and SID of CP and most AA in red dog are greater than in wheat middlings.

Nutritive value of cold-pressed camelina cake with or without supplementation of multi-enzyme in broiler chickens

The objectives were to determine the standardized ileal digestibility (SID) of amino acids (AA) and AMEn value of cold-pressed camelina cake (CPCC) and the effect of adding multi-enzyme to a corn-CPCC diet for broilers. The 600 male broiler chicks were divided into 40 groups and fed 5 diets in a completely randomized design (8 groups per diet) from d 15 to d 21 of age. A corn basal diet and the basal diet with 30% of it replaced by CPCC were used in a 2 × 2 factorial arrangement with or without multi-enzyme (2,800 U of cellulase, 1,800 U of pectinase, 400 U of mannanase, 50 U of galactanase, 1,000 U of xylanase, 600 U of glucanase, 2,500 U of amylase, and 200 U of protease/kilogram of diet; Superzyme OM, 1 g/kg). The fifth diet was N-free. The corn basal diet was fed to determine nutrient digestibility and retention for CPCC by substitution. The N-free diet was fed to estimate basal endogenous AA losses for determining SID of AA. Diets contained TiO2 as indigestible marker. On a DM basis, CPCC contained 39.8% CP, 38.3% neutral detergent fiber, 12.7% ether extract, 1.89% Lys, 0.70% Met, 1.56% Thr, and 0.45% Trp. The SID of Lys, Met, Thr, and Trp for CPCC were 76.5, 85.5, 72.8, and 84.1%, respectively. The AMEn value for CPCC was 1,671 kcal/kg of DM. Multi-enzyme supplementation increased (P < 0.05) the SID of Met and Thr and the AMEn value of the corn-CPCC-based diet by 1.4, 1.3, and 3.0%, respectively. The multi-enzyme increased (P = 0.026) the AMEn value of CPCC from 1,671 to 1,941 kcal/kg of DM. In conclusion, the CPCC evaluated in the present study can be included in poultry diets as a source of energy and AA. Multi-enzyme supplementation increased the AMEn value of CPCC for broilers.

Concentration of digestible, metabolizable, and net energy and digestibility of energy and nutrients in fermented soybean meal, conventional soybean meal, and fish meal fed to weanling pigs

Two experiments were conducted to determine the digestibility of energy and nutrients and the concentration of DE, ME, and NE in fermented soybean meal (FSBM), conventional soybean meal (SBM-CV), and fish meal fed to weanling pigs. In Exp. 1, 36 barrows (initial BW: 22.0 ± 3.85 kg) were placed in metabolism cages and allotted to a randomized complete block design with 4 diets and 9 pigs per diet. Feces and urine were collected for 5 d after a 5 d adaptation period. Four diets including a corn-based diet and 3 diets consisting of corn and each of the experimental ingredients (FSBM, SBM-CV, and fish meal) were formulated. Results indicated that the apparent total tract digestibility (ATTD) of GE in corn, FSBM, and SBM-CV was 88.6, 88.2, and 90.3%, respectively, but the ATTD of GE in fish meal (84.0%) was less (P < 0.01) than in the other ingredients. The concentrations of DE, ME, and NE in SBM-CV were 4,553, 4,137, and 2,972 kcal/kg DM. These values were greater (P < 0.01) than the DE, ME, and NE in FSBM (4,296, 3,781, and 2,710 kcal/kg DM), corn (3,951, 3,819, and 2,791 kcal/kg DM), and fish meal (3,827, 3,412, and 2,450 kcal/kg DM). However, FSBM contained more (P < 0.01) DE, ME, and NE than fish meal and more (P < 0.01) DE than corn. The biological value of the protein in fish meal (75.4%) was greater (P < 0.05) than in corn (34.8%) and FSBM (62.8%), and the biological value of protein in SBM-CV (67.1%) was greater (P < 0.05) than in corn but not different from FSBM and fish meal. In Exp. 2, 8 barrows (initial BW: 10.4 ± 0.47 kg) were equipped with a T-cannula in the distal ileum and randomly allotted to a replicated 4 × 4 Latin square design with 4 diets and 4 periods per square. Three diets containing FSBM, SBM-CV, or fish meal as the sole source of AA and a N-free diet were formulated. The standardized ileal digestibility (SID) of all indispensable AA except Lys, Thr, and Trp was greater (P < 0.01) in FSBM than in fish meal. The SID of Met and Val was also greater (P < 0.05) in FSBM than in SBM-CV, but for the remaining indispensable AA, no difference between FSBM and SBM-CV was observed. In conclusion, the concentration of DE, ME, and NE is less in FSBM than in SBM-CV. However, DE, ME, and NE are greater in FSBM than in fish meal, but the SID of most AA is not different between FSBM and SBM-CV although they are greater than in fish meal.

Ileal digestibility of sunflower meal, pea, rapeseed cake, and lupine in pigs

The standardized ileal digestibility (SID) of CP and AA was evaluated in soybean (Glycine max) meal, sunflower (Helianthus annuus) meal, rapeseed cake, and field pea (Pisum sativum) using 10 pigs and in lupine (Lupinus angustifolius) using 7 pigs. Pigs were fitted with either a T-cannula or a steered ileocecal valve-cannula. Diets contained 170 to 186 g CP/kg DM. Endogenous losses of CP and AA were estimated by feeding a N-free diet. The SID was calculated using the average of Cr(2)O(3) and TiO(2) as indigestible markers and corrected for type of cannula. The SID of CP was greater (P < 0.05) for soybean meal and pea compared to sunflower meal, rapeseed cake, and lupine. The SID of Lys and His were lowest (P < 0.05) in sunflower meal, and the SID of Met and Val were lowest (P < 0.05) in lupine. These results imply soybean meal and pea to be a high-digestible protein source relative to sunflower meal, rapeseed cake, and especially lupine, although all tested feedstuffs seem appropriate for inclusion in diets for organic pigs.

Amino acid digestibility in canola, cottonseed, and sunflower products fed to finishing pigs

Our objective was to determine the standardized ileal digestibility (SID) of CP and AA in soybean meal (SBM) and canola, cotton, and sunflower products fed to finishing pigs. Each of 8 barrows (average initial BW = 106.6 ± 5.5 kg) were surgically fitted with a T-cannula in the distal ileum. Pigs were allotted to an 8 × 8 Latin square design with 8 diets and 8 periods. The 7 protein ingredients were canola seeds (CS), canola meal (CM), cottonseed meal (CSM), sunflower seeds (SFS), sunflower meal (SFM), dehulled sunflower meal (SFM-DH), and SBM, with each ingredient included as the sole source of AA in the diet. A N-free diet was used to estimate basal endogenous losses of AA. Among tested ingredients, SBM had the greatest (P < 0.05) SID of Lys, and CS had the least (P < 0.05) SID of Phe, Thr, and Tyr. The SID of all indispensable AA except Trp was less (P < 0.05) in CS than SBM, and CM had a greater (P < 0.05) SID of all indispensable AA except Arg, His, Lys, and Trp than CS. However, the SID of all indispensable AA except Arg and Trp were less (P < 0.05) in CM than in SBM. The SID of all indispensable AA except Arg and Trp also were less (P < 0.05) in CSM than in SBM, and the SID of Met was less (P < 0.05) in CSM than in all other ingredients. Among sunflower products, the SID of His, Leu, Phe, and Thr were less (P < 0.05) in SFM-DH than in SFS and SFM, and the SID of Ile, Met, and Val were less (P < 0.05) in SFM-DH than in SFS; however, for CP, Arg, Lys, and Trp, no differences among SFS, SFM, and SFM-DH were observed. The SID of all indispensable AA except Trp were less (P < 0.05) in SFM-DH than SBM, and the SID of His, Ile, Lys, Thr, and Val in SFM were also less (P < 0.05) than in SBM. Except for Lys, no differences between SBM and SFS were observed. In conclusion, the SID of most AA in CS, CM, CSM, SFM, and SFM-DH are less than in SBM.