Abstract: The presence of PFSs in urban areas has increased significantly, leading to conflicts with existing land use planning and violations of the minimum environmental safety requirements set by the Department of Petroleum Resources (DPR) and have adverse social and environmental impacts despite their socioeconimic importance. This paper aim to investigate the spatial distribution of petrol filling stations and their impact on groundwater quality in Akure Metropolis of Ondo State. A total of 147 PFSs and 140 water wells were identified and recorded. Laboratory analysis was done purposefully on wells near PFSs that were both 26 years or older and still functional, as older underground storage tanks (USTs) pose a potential threat to groundwater safety. Findings revealed 119 functional filling stations, 28% located within residential area and 68% domiciled in commercial areas, 2% in outskirt of town and 1% in standalone environment. Findings showed all mapped filling stations were found to be within 50m from built up (residential) area, 104 mapped stations within residential and commercial areas violates the DPR standard. Using spatial analysis, it was also found that 116 of the 147 (78.9%) mapped filling stations were within 400m away from the next. 108 of the 147 (73.47%) mapped PFSs violated the standard of distance not less than 15meters from the edge of the road to the nearest pump. The findings also showed that groundwater system is contaminated by PAHs, which comprises; naphthalene.F, pyrene and acenaphthylene.F. Low mean concentrations of PAHs recorded in this study suggest that there is no major compromise in the quality of groundwater due to the operation of PFSs in Akure metropolise Hand-dug Wells in the study area. It is recommended that spatial compliance for siting PFSs should be strictly adhered to in order to avoid disaster that may occur due to the hazard they pose