Stable Isotope Approach Research Articles

Clam diet and production in relation to the spatial pattern of food source inputs and quality: A stable isotope approach

We used four stable isotopes to determine the spatial variability in the diet of Mya arenaria in the Plum Island Sound estuary, Massachusetts, USA and compared diet to the production rate of dietary food sources. Clams were collected for a year along the estuary and their diet determined at each site with four stable isotopes, δ13C, δ15N, δ34S and δ2H, using the Bayesian mixing model (MixSIAR). We compared diet composition and its quality to patterns of clam production. The results showed that M. arenaria depended on locally produced organic matter, the availability of which varied along the estuary. For the lower estuary, the dominant food source for clams was ocean/estuarine unicellular algal particulate organic matter followed by salt marsh detritus. Brackish and riverine organic matter sources were of secondary importance. For the upper estuary, the relative importance of brackish marsh and riverine organic matter increased. The net inputs of organic matter from the river, tidal marshes and unicellular algae were closely matched with the dietary composition of clams. The down estuary pattern of increasing clam productivity was best predicted by a similar pattern in suspended particulate organic matter quality as indicated by the ratio of C:N. The C:N ratio decreased from about 10.5:1 to 7.4:1 along the region of clam growth. We also found that δ2H was a useful tracer, effectively discriminating between marsh-derived and aquatic algae-derived organic matters and significantly increasing the predictive power and reliability of the mixing model. Our study provides fundamental knowledge on suitable habitats for the M. arenaria growth in the Plum Island estuary and insights into selecting stable isotopes for elucidating feeding ecology and trophic dynamics of estuarine organisms.

Hydrochemical and Isotopic Characterization of Groundwater in the Nakivale Sub-Catchment of the Transboundary Lake Victoria Basin, Uganda

This study characterized groundwater resources for the Nakivale sub-catchment of the transboundary Victoria Basin in Uganda using classical hydrochemical and stable isotopic approaches. Groundwater in the study area is essential for domestic, agricultural, and industrial uses. As a sub-domain of the larger Victoria Basin, it also plays a crucial role in shaping the hydrological characteristics of this vital transboundary basin, both in terms of quality and quantity fronts. This makes its sustainable management and development vital. The predominant groundwater type is Ca-SO4, with other types including Ca-HCO3, Na-Cl, Na-HCO3, and Ca-Mg-SO4-Cl. Hydrochemical facies analysis highlights the importance of rock–water interactions in controlling groundwater chemistry, mainly through incongruent chemical weathering of Ca-rich plagioclase feldspars and the oxidation of sulfide minerals, such as pyrite, which are prevalent in the study area. Groundwater recharge is primarily influenced by the area’s topography, with recharge zones characterized by lineament networks, located in elevated areas. Stable isotope analyses indicate that groundwater mainly originates from local precipitation, while tritium data suggest the presence of both recent and older groundwater (likely over 20 years old). The study’s comprehensive approach and findings contribute significantly to the understanding of groundwater systems in the region, thus providing valuable insights for policymakers and stakeholders involved in water resource management and development strategies.

Karst leakage analysis of Roudbal Dam, southwestern Iran, using geological, hydrogeological, hydrochemical and stable-isotopic approaches

Karst leakage analysis of Roudbal Dam, southwestern Iran, using geological, hydrogeological, hydrochemical and stable-isotopic approaches

Hydrochemical Characteristics and Formation Mechanism of Geothermal Fluids in Zuogong County, Southeastern Tibet

Zuogong County is located in the southeast of Tibet, which is rich in hot spring geothermal resources, but its development and utilization degree are low, and the genetic mechanism of the geothermal system is not clear. Hydrogeochemical characteristics of geothermal water are of great significance in elucidating the genesis and evolution of geothermal systems, as well as the sustainable development and utilization of geothermal resources. The hydrogeochemical characteristics and genesis of the geothermal water in Zuogong County were investigated using hydrogeochemical analysis, a stable isotope (δD, δ18O) approach, and an inverse simulation model for water–rock reactions using the PHREEQC. The results indicated that the Zuogong geothermal system is a deep circulation heating type without a magmatic heat source. The chemical types present in the geothermal water from the Zuogong area are HCO3 and HCO3·SO4, and the main cations are Na+ and Ca2+. The groundwater is replenished by atmospheric precipitation and glacier meltwater. The salt content of geothermal water mainly comes from the interaction between water and surrounding rocks during the deep circulation process. The reservoir temperature of geothermal water in Zuogong is 120–176 °C before mixing with non-geothermal water and drops to 62–98 °C after mixing with 58 to 79% of non-geothermal water. According to the proposed conceptual model, geothermal water mainly produces water–rock interaction with aluminosilicate minerals in the deep formation, while in shallow areas it interacts mainly with sulfate minerals. These findings contribute to a better understanding of the geothermal system in Zuogong County, Tibet.

Extreme precipitation reduces the recent photosynthetic carbon isotope signal detected in ecosystem respiration in an old-growth temperate forest.

The successful utilization of stable carbon isotope approaches in investigating forest carbon dynamics has relied on the assumption that the carbon isotope compositions (δ13C) therein have detectable temporal variations. However, interpreting the δ13C signal transfer can be challenging, given the complexities involved in disentangling the effect of a single environmental factor, the isotopic dilution effect from background CO2 and the lack of high-resolution δ13C measurements. In this study, we conducted continuous in situ monitoring of atmospheric CO2 (δ13Ca) across a canopy profile in an old-growth temperate forest in northeast China during the normal year 2020 and the wet year 2021. Both years exhibited similar temperature conditions in terms of both seasonal variations and annual averages. We tracked the natural carbon isotope composition from δ13Ca to photosynthate (δ13Cp) and to ecosystem respiration (δ13CReco). We observed significant differences in δ13Ca between the two years. Contrary to in 2020, in 2021 there was a δ13Ca valley in the middle of the growing season, attributed to surges in soil CO2 efflux induced by precipitation, while in 2020 values peaked during that period. Despite substantial and similar seasonal variations in canopy photosynthetic discrimination (Δ13Ccanopy) in the two years, the variability of δ13Cp in 2021 was significantly lower than in 2020, due to corresponding differences in δ13Ca. Furthermore, unlike in 2020, we found almost no changes in δ13CReco in 2021, which we ascribed to the imprint of the δ13Cp signal on above-ground respiration and, more importantly, to the contribution of stable δ13C signals from soil heterotrophic respired CO2. Our findings suggest that extreme precipitation can impede the detectability of recent photosynthetic δ13C signals in ecosystem respiration in forests, thus complicating the interpretation of above- and below-ground carbon linkage using δ13CReco. This study provides new insights for unravelling precipitation-related variations in forest carbon dynamics using stable isotope techniques.

Kinetic Modeling of In Vivo K+ Distribution and Fluxes with Stable K+ Isotopes: Effects of Dietary K+ Restriction.

Maintaining extracellular potassium (K+) within narrow limits, critical for membrane potential and excitability, is accomplished through the internal redistribution of K+ between extracellular fluid (ECF) and intracellular fluid (ICF) in concert with the regulation of renal K+ output to balance K+ intake. Here we present evidence from high-precision analyses of stable K+ isotopes in rats maintained on a control diet that the tissues and organs involved in the internal redistribution of K+ differ in their speed of K+ exchange with ECF and can be grouped into those that exchange K+ with ECF either rapidly or more slowly ("fast" and "slow" pools). After 10 days of K+ restriction, a compartmental analysis indicates that the sizes of the ICF K+ pools decreased but that this decrease in ICF K+ pools was not homogeneous, rather occurring only in the slow pool (15% decrease, p < 0.01), representing skeletal muscles, not in the fast pool. Furthermore, we find that the dietary K+ restriction is associated with a decline in the rate constants for K+ effluxes from both the "fast" and "slow" ICF pools (p < 0.05 for both). These results suggest that changes in unidentified transport pathways responsible for K+ efflux from ICF to ECF play an important role in buffering the internal redistribution of K+ between ICF and ECF during K+ restriction. Thus, the present study introduces novel stable isotope approaches to separately characterize heterogenous ICF K+ pools in vivo and assess K+ uptake by individual tissues, methods that provide key new tools to elucidate K+ homeostatic mechanisms in vivo.

Slow recovery in trophic structure of restored wetlands in Northeast China

Restoration measures have been widely implemented in wetland ecosystems globally to bend the curve of biodiversity loss and restore associated ecological functions. However, assessments of the effectiveness of wetland restoration have predominantly focused on the recovery of taxonomic composition, while few studies have assessed the effectiveness of these efforts from a food web perspective. Here, we incorporated stable isotope approach to investigate trophic structure in natural and restored wetlands in Northeast China. The investigated consumers, including zooplankton, macroinvertebrates, and fish, exhibited lower δ15N and higher δ13C values in restored wetlands than in natural wetlands. Natural wetlands exhibited higher trophic positions and a wider range of trophic levels compared to restored wetlands. Primary consumers in natural wetlands relied more on particulate organic matter (POM, 42.9 % ± 24.1 %), while those in restored wetlands were more dependent on substrate organic matter (SOM, 42.3 % ± 23.9 %). Compared to natural wetlands, isotopic richness was significantly lower in restored wetlands, with smaller isotopic variation (SEAs) in basal resources, aquatic invertebrates, and fish. Our findings reveal that the recovery of trophic structures in restored wetlands lags behind that of taxonomic composition. Future restoration efforts should prioritize enhancing habitat heterogeneity and resource availability to support a diverse range of trophic levels. Monitoring trophic dynamics is essential for assessing the progress of wetland restoration and should be integrated into monitoring schemes.

Drought effects in Mediterranean forests are not alleviated by diversity‐driven water source partitioning

Abstract Tree species diversity in forest ecosystems could reduce their vulnerability to extreme droughts through improved microclimate and below‐ground water source partitioning driven by contrasting species‐specific water use patterns. However, little is known about the seasonal dynamics of belowground water uptake that determine whether diversity positively or negatively impacts tree carbon assimilation and water exchange. Using a network of 30 permanent plots in Mediterranean forests with increasing tree species diversity (from monospecific to four‐species mixtures), we examined the seasonal patterns of in‐situ aboveground carbon and water relations and belowground water sources on 265 trees from four pine and oak species over 2 years using hydraulic and stable isotope approaches. We found that increasing species diversity in broadleaf and conifer mixtures induced strong soil water source partitioning between oak and pine species. As conditions became drier during the summer in mixed stands, oak species took up water from deeper soil sources, while pines were systematically limited to shallow ones. Despite significant belowground moisture partitioning, stronger drought‐induced reductions in photosynthesis, stomatal conductance, and leaf water potential were still observed in diverse compared with monospecific stands for pines but with some benefits for oaks. Synthesis: Our findings reveal that tree species diversity promoted belowground water source partitioning in mixed oak and pine stands, potentially reducing competition for water in more diverse ecosystems. Yet, our results show that it is insufficient to buffer the adverse impacts of severe droughts on aboveground tree carbon and water use, leading to higher water stress, especially for pines.

To eat or not to eat: novel stable isotope models reveal a shift in carnivory with nutrient availability for aquatic Utricularia spp.

Freshwater nitrogen inputs are increasing globally, altering the structure and function of wetland ecosystems adapted to low nutrient conditions. Carnivorous wetland plants of the genus Utricularia are hypothesized to reduce their reliance on carnivory and increase their assimilation of environmental nutrients when the supply of ambient nutrients increases. Despite success in using stable isotope approaches to quantify carnivory of terrestrial carnivorous plants, quantifying carnivory of aquatic Utricularia requires improvement. We developed stable isotope mixing models to quantify aquatic plant carnivory and used these models to measure dietary changes of three Utricularia species, Utricularia australis, U. gibba and U. uliginosa, in 11 wetlands across a 794-km gradient in eastern Australia. Diet was assessed using multiple models that compared variations in the natural-abundance nitrogen isotope composition (δ15N) of Utricularia spp. with that of non-carnivorous plants, and environmental and carnivorous nitrogen sources. Carnivory supplied 40-100 % of plant nitrogen. The lowest carnivory rates coincided with the highest availability of ammonium and dissolved organic carbon. Our findings suggest that Utricularia populations may adapt to high nutrient environments by shifting away from energetically costly carnivory. This has implications for species conservation as anthropogenic impacts continue to affect global wetland ecosystems.

Evaporation from dams governing the water cycle dynamics of a regulated river basin from the Western Ghats: Sharavati, India

Study regionSharavati River, Karnataka, India. Study focusA small mountainous river system, Sharavati, was selected to study the impact of river damming on the hydrological cycle. Sharavati river flow is regulated by two dams, Linganamakki and Gersoppa. Despite the Western Ghats' global significance in controlling local and regional climates, the effects of damming on its hydrological cycles have received limited attention. A stable water isotopic approach was employed in the study. New hydrological insights for the regionThe line-conditioned excess (lc-excess) was primarily negative across all seasons. Notably, the pre-monsoon season exhibited comparatively higher evaporation with high negative lc-excess, while the postmonsoon lc-excess values approached zero, indicating minimal evaporation. The sampling points from the dams exhibited very high evaporation signals, the evaporative loss during the pre-monsoon season from the Linganamakki reservoir was estimated as 10 %, and from the Gersoppa dam was 6 %. Consequently, groundwater sampled near the dams, plotted along the local evaporation line indicating recharge from the evaporated reservoir water. Damming has affected the hydrological cycle of the heavily regulated Sharavati River, transforming the entire catchment into a connected, narrow lake-like structure, especially during the pre-monsoon season. Since the Western Ghat river systems are regulated by many large and small dams, it is pertinent to study the impact of damming on the hydrological cycles of the entire system.

Erosion of fish trophic position: an indirect effect of fishing on food webs elucidated by stable isotopes.

Fishing has significant trophodynamic impacts on marine communities, including reductions in the mean trophic position (TP) of the ecosystem resulting from a decrease in the abundance and size of species and individuals with high TPs. This study demonstrates the erosion of fish TP, an additional process that results in lower TP of individuals of a given size, which may exacerbate the effects of fishing on the food web. A stable isotope approach based on the tRophicPosition Bayesian method was used to quantify the TP of 12 target marine species at a given length, and compare their TP between fishery-restricted areas and trawled areas. The results show a difference in the TP of six benthic and apical nekto-benthic predators, which feed in the median at about 0.5 TP lower in trawled areas. It appears that current 'fishing down marine food webs' analyses may underestimate the trophic effects of fishing. Accounting for changes in trophodynamics of individuals at a given size is important to detect indirect effects through food web interactions. The application of a trawling ban may lead to the restoration of lost trophic structure; however, trophic changes may occur more slowly than changes in biomass. This article is part of the theme issue 'Connected interactions: enriching food web research by spatial and social interactions'.

Comparison with carbonate rocks hosted iron skarn in the eastern Sakarya Zone (Gümüşhane, NE Turkey): a geomorphologic, geochemical and stable isotopic approach

Comparison with carbonate rocks hosted iron skarn in the eastern Sakarya Zone (Gümüşhane, NE Turkey): a geomorphologic, geochemical and stable isotopic approach

Iron absorption in adults with sickle cell anemia: a stable-isotope approach.

Iron absorption in sickle cell anemia (SCA) remains unclear and studies in adults with SCA are scarce. The aim of this study was to evaluate the iron absorption SCA adults and its association with iron status and hepcidin concentration. SCA patients (n = 13; SCAtotal) and control participants (n = 10) ingested an oral stable iron isotope (57Fe). Iron absorption was measured by inductively coupled plasma mass spectrometry (ICP-MS) 14 days after isotope administration. Patients with ≥ 1000 ng/mL serum ferritin were considered to present iron overload (IO) (SCAio+; n = 3) and others classified without IO (SCAio-; n = 10). Iron absorption in the control group ranged from 0.3 to 26.5% (median = 0.9%), while it varied from 0.3 to 5.4% in SCAio+ (median = 0.5%) and from 0.3 to 64.2% in the SCAio- (median = 6.9%). Hepcidin median values were 14.1 ng/mL (3.0-31.9 ng/mL) in SCAio-, 6.2 ng/mL (3.3-7.8 ng/mL) in SCAio + and 6.2 ng/mL (0.6-9.3 ng/mL) in control. Iron absorption was associated with ferritin level (r = - 0.641; p = 0.018) and liver iron concentration (LIC; r = - 0.786; p = 0.036) in the SCAtotal group. Our data suggest that SCAio- individuals may be at risk of developing primary IO. Simultaneously, secondary IO may induce physiological adaptation, resulting in reduced iron absorption. Further studies evaluating intestinal iron absorption using larger sample sizes should be conducted to help establish a safe nutrition approach to be adopted and to ensure the security of food-fortifying public policies for these patients. This trial was registered at www.ensaiosclinicos.gov.br (Identifier RBR-4b7v8pt).

Compartmental analysis: a new approach to estimate protein breakdown and meal response in health and critical illness.

This study aimed to discuss the use of the pulse stable isotope tracer approach to study changes in metabolism in healthy individuals and critically ill patients. We found that in the postabsorptive state and healthy condition, intracellular protein breakdown and net intracellular protein breakdown, when calculated using the pulse tracer approach, are about double what has previously been reported using the more traditional primed-constant and continuous stable isotope approaches (600 versus 300 grams of protein/day). In critically ill patients, protein breakdown is even higher and calculated to be approximately 900 grams of protein/day, using the pulse tracer approach. Based on these data, we hypothesize that reducing protein breakdown in the postabsorptive state is key when trying to improve the condition of critically ill patients. Moreover, we also used the pulse tracer approach during feeding to better estimate the intracellular metabolic response to feeding. Our first observation is that endogenous protein breakdown does not seem to be reduced during feeding. We also have shown that when consuming a meal with a certain amount of protein, the biological value of that protein meal can be calculated with the pulse tracer approach. In conclusion, using the pulse stable isotope tracer approach to study protein kinetics in the postabsorptive state and during feeding expands our understanding of how dietary proteins can affect human protein metabolism. The intracellular protein synthesis stimulatory effect of a meal is an important factor to consider when calculating the exact protein requirements and needs, particularly in critical illness.

Contribution of carbonate-derived dissolved inorganic carbon into autochthonous particulate organic carbon in two small temperate Korean rivers (Geum and Seomjin)

In this study, we estimated the contributions of carbonate mineral weathering to dissolved inorganic carbon (DIC) and carbonate-derived DIC to autochthonous particulate organic carbon (POC) in two temperate Korean rivers. We combined stoichiometric and stable carbon isotopic approaches to calculate the contribution of autochthonous POC, considering diverse riverine DIC sources. We collected surface water samples from May 2016 to May 2018 and analyzed the major ion composition of rivers along with the concentrations and stable carbon isotopes of DIC. Our estimates showed that the relative abundances of carbonate mineral weathering (0.41 ± 0.11 in the Geum River and 0.43 ± 0.07 in the Seomjin River) were only slightly lower than those of silicate mineral weathering (0.59 ± 0.1 in the Geum River and 0.57 ± 0.07 in the Seomjin River). The resulting percentage contributions of DIC derived from the carbonate mineral weathering to riverine autochthonous POC, if we consider the additional DIC sources of atmospheric and soil-derived CO2, were 10 ± 3 % in the Geum River and 2 ± 1 % in the Seomjin River. The calculated annual fluxes of carbonate-derived DIC for 2016–2018 were 23.2 ± 0.3 Gg C yr−1 in the Geum River and 1.1 ± 0.4 Gg C yr−1 in the Seomjin River. Moreover, the calculated annual fluxes of carbonate-derived POC were 3.6 ± 0.5 Gg C yr−1 in the Geum River and 0.1 ± 0.7 Gg C yr−1 in the Seomjin River. Accordingly, our study provides the first insight into the contribution of carbonate-derived DIC to riverine autochthonous POC in small temperate Korean river systems, dominated by silicate rocks.

Trophic ecology of the greater amberjack in relation to marine environmental characteristics in Taiwanese waters illustrated by stable isotope and stomach content approach

The greater amberjack (Seriola dumerili) holds significant commercially and ecologically important large pelagic marine species in Taiwanese waters. The aim of this study was investigates its stomach contents and stable isotope signatures (δ13C and δ15N) were used to explore the trophic ecology and to detect the dietary variations by length, sex, and season and their associations with marine environmental characteristics. Utilizing a generalized additive model, we elucidate how marine environmental variables influence greater amberjack feeding habitats. Our findings revealed that the mixed layer depth and sea surface temperature explained the highest influence in the greater amberjack feeding ground. To reveal the trophic ecology of the greater amberjack and their ecosystem trophic dynamics, we used an innovative approach combining stomach content analysis (SCA) and stable isotope analysis (SIA) of carbon and nitrogen (δ13C and δ15N) coupled with Bayesian mixing model analysis. By contrast, some of the results in SIA were different from the most important food items identified in SCA; in general, they prefer to consume pelagic prey species. The Bayesian mixing models indicated that the target predator species diet structure was dominated by Trichiuridae and Leiognathidae prey species, as indicated by the highest δ15N values. The relationships of the greater amberjack weight and length were not significant with δ13C values (p > 0.05) but were significant (p < 0.001) with δ15N values. The relationships between trophic positions (TPs) in terms of length, sex, and season suggested an ontogenetic shift, with varied TP values between 4.01 and 5.18, indicating that the greater amberjack is the top-level predator species in the pelagic marine ecosystem. These findings can guide ecosystem-based adaptive fishery management and conservation because the local prey species depletion and variations in marine environmental conditions may affect greater amberjack fisheries in Taiwanese waters.

Evapotranspiration partitioning through water stable isotopic measurements in a subtropical coniferous forest

AbstractEvapotranspiration (ET) partitioning distinguishes the soil evaporation (E) and plant transpiration (T) components and is crucial for understanding the land‐atmosphere interactions and ecosystem water budget. However, the mechanism and controls of ET partitioning for subtropical forests in heterogeneous environments remain poorly understood. Here, we present δ18O and δ2H of about 1,527 isotope samples including atmospheric water, soil and plant water during different seasons in 2 years of 2020–2021 from a coniferous forest across Southeast China. We used the isotopic mass balance of ecosystem water pools, the Craig‐Gordon model and the Keeling‐Plot method to partition T from ET (T/ET) and quantify the controls on T/ET. Results indicated that the uncertainty in the T/ET was principally from the soil water evaporation (δE) value, about 20–30 cm was found to be a reasonable evaporating front depth for estimating δE in this coniferous forest. T/ET presented a “U” shape diurnal pattern and varied from 66.7% to 89.9%. Isotope‐based T/ET in autumn with high temperatures and little rain was higher than those in the summer and winter seasons. Relative humidity (or vapour pressure deficit) dominated the diurnal T/ET variations (relative contributions of &gt; 40%) in summer and autumn, while air temperature and soil water content were the main controls in winter. Our study also showed that δ18O‐derived T/ET was consistent with that of δ2H, although δ2H was found to be more stable in ET partitioning, the dual stable isotope approach should be employed in future studies for the uncertainties brought by samplings or measurements. The agreement between the isotope‐based T/ET and ET partitioning approach that uses eddy covariance and sap flux data was stronger at midday. These isotope‐inferred ET partitioning can inform land surface models and provide more insights into water management in subtropical forests.

Combined stomach contents and stable isotope analysis reveal feeding habits of Japanese jack mackerel (Trachurus japonicus) in the East China Sea

Japanese jack mackerel (Trachurus japonicus) is an economically important pelagic fish in the East China Sea. Knowledge is still limited regarding the feeding habits of this species in this area. Based on bottom trawl surveys conducted in the southern coastal area of Zhejiang during August in 2019 and 2020, we analyzed the feeding ecology of T. japonicus using stomach contents analysis and the stable isotope approach. The results indicated that the δ13C value for T. japonicus ranged from − 17.72 % to ;−14.30 %, and the δ15N value ranged from 10.89 % to 13.03 %. Two-way analysis of variance indicated significant annual and ontogenetic variations in the δ13C value, thereby suggesting changes in the final nutrient sources for T. japonicus in different growth stages and years. The trophic level of T. japonicus was estimated as from 3.35 to 4.46. In general, the trophic level of T. japonicus increased with growth, although the ontogenetic variation was not significant. The main food groups identified for T. japonicus based on stomach contents analysis were fish, shrimp, crabs, cephalopods, and small crustaceans. The results obtained by stable isotope analysis showed that the nutrient contribution rate of small crustaceans to the diet of T. japonicus was highest in both years, followed by shrimp. The feeding habits of T. japonicus had obvious ontogenetic variations, and the average contribution of fish species increased as the size of the predator increased. The results obtained in this study provide detailed feeding information about T. japonicus in the East China Sea.

Serine synthesis and catabolism in starved lung cancer and primary bronchial epithelial cells

Serine and glycine give rise to important building blocks in proliferating cells. Both amino acids are either synthesized de novo or taken up from the extracellular space. In lung cancer, serine synthesis gene expression is variable, yet, expression of the initial enzyme, phosphoglycerate dehydrogenase (PHGDH), was found to be associated with poor prognosis. While the contribution of de novo synthesis to serine pools has been shown to be enhanced by serine starvation, the impact of glucose deprivation, a commonly found condition in solid cancers is poorly understood. Here, we utilized a stable isotopic tracing approach to assess serine and glycine de novo synthesis and uptake in different lung cancer cell lines and normal bronchial epithelial cells in variable serine, glycine, and glucose conditions. Under low glucose supplementation (0.2 mM, 3–5% of normal plasma levels), serine de novo synthesis was maintained or even activated. As previously reported, also gluconeogenesis supplied carbons from glutamine to serine and glycine under these conditions. Unexpectedly, low glucose treatment consistently enhanced serine to glycine conversion, along with an up-regulation of the mitochondrial one-carbon metabolism enzymes, serine hydroxymethyltransferase (SHMT2) and methylenetetrahydrofolate dehydrogenase (MTHFD2). The relative contribution of de novo synthesis greatly increased in low serine/glycine conditions. In bronchial epithelial cells, adaptations occurred in a similar fashion as in cancer cells, but serine synthesis and serine to glycine conversion, as assessed by label enrichments and gene expression levels, were generally lower than in (PHGDH positive) cancer cells. In summary, we found a variable contribution of glucose or non-glucose carbon sources to serine and glycine and a high adaptability of the downstream one-carbon metabolism pathway to variable glucose supply.

Mercury stable isotopes in seabirds in the Ebro Delta (NE Iberian Peninsula): Inter-specific and temporal differences

Mercury (Hg) is a global pollutant, which particularly affects aquatic ecosystems, both marine and freshwater. Top-predators depending on these environments, such as seabirds, are regarded as suitable bioindicators of Hg pollution. In the Ebro Delta (NE Iberian Peninsula), legacy Hg pollution from a chlor-alkali industry operating in Flix and located ca. 100 km upstream of the Ebro River mouth has been impacting the delta environment and the neighboring coastal area. Furthermore, levels of Hg in the biota of the Mediterranean Sea are known to be high compared to other marine areas. In this work we used a Hg stable isotopes approach in feathers to understand the processes leading to different Hg concentrations in three Laridae species breeding in sympatry in the area (Audouin's gull Ichthyaetus audouinii, black-headed gull Chroicocephalus ridibundus, common tern Sterna hirundo). These species have distinct trophic ecologies, exhibiting a differential use of marine resources and freshwater resources (i.e., rice paddies prey). Moreover, for Audouin's gull, in which in the Ebro Delta colony temporal differences in Hg levels were documented previously, we used Hg stable isotopes to understand the impact of anthropogenic activities on Hg levels in the colony over time. Hg stable isotopes differentiated the three Laridae species according to their trophic ecologies. Furthermore, for Audouin's gull we observed temporal variations in Hg isotopic signatures possibly owing to anthropogenic-derived pollution in the Ebro Delta. To the best of our knowledge this is the first time Hg stable isotopes have been reported in seabirds from the NW Mediterranean.