δ13C Stable Isotope Analysis Research Articles

At the base of deep-sea food webs: Assemblage and trophic structure of suprabenthos and zooplankton in submarine canyons

Submarine canyons act as hotspots of biodiversity, hosting vulnerable marine ecosystems, and playing a fundamental role in bridging coastal zones with deeper areas. Here, we investigated the suprabenthic and Deep Scattering Layer (DSL) zooplankton fauna, that play a key role in deep-sea food webs, as main resources for both mobile and sessile megafauna, in two submarine canyons (Squillace and Amendolara) of the Ionian Sea (Central Mediterranean Sea). Our results highlighted different taxonomic and functional diversity between the two adjacent canyons: i) biomass and abundance of suprabenthos followed an opposite trend in the two canyons, increasing both with depth in Amendolara (higher abundance and biomass in the lower part of the canyon), and decreasing with depth in Squillace (greater in the head of the canyon); ii) DSL zooplankton abundance and biomass followed a spatial distribution, decreasing with increasing distance from the coast for both canyons (i.e. lower offshore than at the head of the canyon). Food-web structure investigated by means of stable isotope analysis of δ13C and δ15N showed a more diverse trophic niche for suprabenthos than for zooplankton. Furthermore, possible feeding modes of species with unknown feeding behaviour have been proposed. The results of the current article highlight the different ecological processes occurring within each canyon. Understanding the spatial variations of communities inhabiting submarine canyons, especially those at the base of deep-sea food webs which can act as driver of megafaunal communities (both sessile and mobile-commercial species), is essential to focalise future conservation efforts.

Complex genesis of multiperiod fractures in the Middle Triassic Leikoupo Formation in the Pengzhou gas field, western Sichuan Basin, China

The cumulative expression of multistage deformation is complex multiperiod fractures, which are commonly seen in tectonic zones. The Middle Triassic Leikoupo Formation in the western Sichuan Basin Depression, China, is a typical marine carbonate reservoir with natural fractures caused mainly by tectonic movements. According to outcrops, drill cores, image logging, and fluid inclusions, the fracture characteristics, types of natural fractures, and interactions of fractures are determined. In total, 419 natural fractures in 493.2 m of cores from 7 wells are investigated, which are mainly shear and tensile fractures with a small number of weathering generated fractures. Meanwhile, the results of the stable isotope analysis of δ13C and δ18O, as well as the flow fluid inclusion data, reveal four tectonic periods of fractures with different occurrences. Based on the history of regional tectonic evolution, indicating one period of weathering fractures ascribable to stratal uplift and three periods of structural fractures related to the sequential tectonic movements of the Longmenshan fault belt. By analyzing the interaction relationships of fractures, three types of fracture interaction relationships are observed: cutting, restraining, and overlapping. The four stages fractures are chronologically assigned to (1) the early Indosinian N–S trending compression, (2) the late Indosinian NW-SE compression, (3) the middle Yanshanian NE-SE compression, and (4) the early Himalayan E-W compression. The influence of natural fractures on gas migration and well production in marine carbonates is discussed, and indicates that tectonic fractures could provide seepage channels for gas migration and accumulation from near or distant hydrocarbon source rocks into the Middle Triassic Leikoupo Formation. This study utilizes a pragmatic approach for understanding the fracture genesis mechanism in oil and gas field with multiperiod fractures.

Determination of the geographical origin of Trachinotus ovatus and Pampus argenteus in China by multi-element and stable isotope analysis

This study aimed to determine whether Trachinotus ovatus and Pampus argenteus could be differentiated by multi-element composition or stable isotope ratio analysis of δ13C and δ15N. The multi-element analysis values of a total of 60 Pampus argenteus samples from three sites and 60 Trachinotus ovatus samples from four sites around China were determined, and the other 60 samples of Pampus argenteus and 51 samples of Trachinotus ovatus were tested by principal component analysis (PCA) to estimate the accuracy of origin identification. The results showed that the cross-validation accuracy rate is 92.2% for the Pampus argenteus and 98.3% for the Trachinotus ovatus. Stable isotope analysis of δ13C and δ15N also can distinguish Trachinotus ovatus from different geographical origins. These results showed that the usefulness of multi-element and stable isotope analysis as indicators for authenticating the geographical origin of two pomfrets in China.

Contribution of emergent aquatic insects to the trophic variation of tropical birds and bats

Food webs are spatially connected by cross-ecosystem fluxes of resources, especially in aquatic-terrestrial boundaries. Generalist consumers are often supported by allochthonous resources, which can influence their density, biomass, and distribution. In this study, we investigated the influence of allochthonous aquatic resources on the foraging activity of bats (by ultrasound emissions) and richness of birds (by birdsong records). We also used stable isotope analysis of δ13C and δ15N to determine their diet and trophic space using bayesian Standard Ellipse Area (SEA). We evaluated these responses in near-site (0-1 m from stream margin) and far-site (200 m from stream margin) from six streams in the Atlantic and the Amazon Forest. Foraging activity of bats and richness of Amazonian birds were higher in near-sites compared to far-sites, while Atlantic bird richness was higher in far-sites. We found that emergent aquatic insects and spiders contributed to 46–45% and 49–36% of the diet of insectivorous bats and birds, respectively, regardless of the distance from streams. In Atlantic Forest, SEA of both birds and bats were not compared between plots because of the low sample size. In Amazon Forest, the far-site trophic space of birds showed a higher δ15N position in the bi-plot. These findings suggest that allochthonous resources are important for areas near and far from streams. Our study underscores the importance of preserving both near and adjacent far habitats in the maintenance of trophic interaction involving flying vertebrates and highlights the interdependence of those areas via allochthonous subsidies.

Inter-Specific and Intra-Specific Competition of Two Sympatrically Breeding Seabirds, Chinstrap and Gentoo Penguins, at Two Neighboring Colonies.

Simple SummaryUnder limited resources, different species may coexist by segregating their food items and foraging time, but neighboring colonies within the same species may have highly overlapping foraging habits. Thus, it is hypothesized that intra-specific competition is more intense than the inter-specific competition. To test this hypothesis, we chose sympatrically breeding Chinstrap and Gentoo penguins at two neighboring colonies and tracked their foraging space and dive depths during chick-rearing. Here we report that there was a larger overlap in the foraging space between the two species than within each species, whereas there was lower inter-specific isotopic niche overlap than intra-specific isotopic niche overlap. Despite the low intra-specific spatial overlap, diets of conspecifics remained similar, resulting in the higher isotopic niche overlaps. Our results suggest that intra-specific competition is higher than inter-specific competition due to a lack of niche partitioning.Theory predicts that sympatric predators compete for food under conditions of limited resources. Competition would occur even within the same species, between neighboring populations, because of overlapping foraging habits. Thus, neighboring populations of the same species are hypothesized to face strong competition. To test the hypothesis that intra-specific competition is more intense than inter-specific competition owing to a lack of niche partitioning, we estimated the foraging area and diving depths of two colonial seabird species at two neighboring colonies. Using GPS and time-depth recorders, we tracked foraging space use of sympatric breeding Chinstrap and Gentoo penguins at Ardley Island (AI) and Narębski Point (NP) at King George Island, Antarctica. GPS tracks showed that there was a larger overlap in the foraging areas between the two species than within each species. In dive parameters, Gentoo penguins performed deeper and longer dives than Chinstrap penguins at the same colonies. At the colony level, Gentoo penguins from NP undertook deeper and longer dives than those at AI, whereas Chinstrap penguins did not show such intra-specific differences in dives. Stable isotope analysis of δ13C and δ15N isotopes in blood demonstrated both inter- and intra-specific differences. Both species of penguin at AI exhibited higher δ13C and δ15N values than those at NP, and in both locations, Gentoo penguins had higher δ13C and lower δ15N values than Chinstrap penguins. Isotopic niches showed that there were lower inter-specific overlaps than intra-specific overlaps. This suggests that, despite the low intra-specific spatial overlap, diets of conspecifics from different colonies remained more similar, resulting in the higher isotopic niche overlaps. Collectively, our results support the hypothesis that intra-specific competition is higher than inter-specific competition, leading to spatial segregation of the neighboring populations of the same species.

Investigating Neolithic caprine husbandry in the Central Pyrenees: Insights from a multi-proxy study at Els Trocs cave (Bisaurri, Spain).

Sheep remains constitute the main archaeozoological evidence for the presence of Early Neolithic human groups in the highlands of the Southern Pyrenees but understanding the role of herding activities in the Neolithisation process of this mountain ecosystem calls for the analysis of large and well-dated faunal assemblages. Cova de Els Trocs (Bisaurri, Huesca, Spain), a cave located at 1564 m a.s.l on the southern slopes of the Central Pyrenees, is an excellent case study since it was seasonally occupied throughout the Neolithic (ca. 5312-2913 cal. BC) and more than 4000 caprine remains were recovered inside. The multi-proxy analytical approach here presented has allowed us to offer new data elaborating on vertical mobility practices and herd management dynamics as has not been attempted up until now within Neolithic high-mountain sites in the Iberian Peninsula. For the first time, δ18O and δ13C stable isotope analyses offer direct evidence on both the regular practice of altitudinal movements of sheep flocks and the extended breeding season of sheep. Autumn births are recorded from the second half of the fifth millennium cal. BC onwards. Age-at-death distributions illustrate the progressive decline in caprine perinatal mortality together with the rising survival rate of individuals older than six months of age and the larger frequency of adults. This trend alongside the 'off-season' lambing signal at the implementation of husbandry techniques over time, probably aiming to increase the size of the flocks and their productivity. Palaeoparasitological analyses of sediment samples document also the growing reliance on herding activities of the human groups visiting the Els Trocs cave throughout the Neolithic sequence. In sum, our work provides substantial arguments to conclude that the advanced herding management skills of the Early Neolithic communities arriving in Iberia facilitated the anthropisation process of the subalpine areas of the Central Pyrenees.

European lobsters utilise Atlantic salmon wastes in coastal integrated multi-trophic aquaculture systems

In this study, we investigated if juvenile European lobstersHomarus gammaruswould eat waste from Atlantic salmonSalmo salarcages in a coastal integrated multi-trophic aquaculture (IMTA) setup and if there were any impacts on growth. Trophic interactions between salmon and lobsters were assessed using δ15N and δ13C stable isotope analysis and fatty acid profiling from fish feed as indicators of nutrient flow. Analysis revealed that lobsters directly utilised particulate waste from salmon production, as levels of indicator fatty acids from salmon feed were significantly higher in lobster tissues near the fish cages compared to the control site. Route of uptake may have been direct consumption of waste feed or faecal material or indirectly through fouling organisms. Stable isotope analysis did not indicate nutrient transfer to lobsters, suggesting that the duration of the study and/or the amount of waste consumed was not sufficient for stable isotope analysis. Lobsters grew significantly over the trial period at both sites, but there was no significant difference in lobster growth between the sites. Our results show a trophic relationship between salmon and lobsters within this IMTA system, with no apparent advantage or disadvantage to growth.

Feeding preferences in aquatic invertebrates associated to Egeria densa in a tropical high-mountain lake

Introduction: The benthic fauna of the littoral zone in lakes is important in transferring energy to other trophic levels, habitat coupling, and helping to keep habitat stability. The study of this type of interactions in lentic systems is priority, since functional aspects about biological communities are unknown. Objective: Describing the trophic relations of aquatic invertebrates of the littoral zone in a tropical high mountain lake by characterizing gut content and stable isotope analysis of δ13C and δ15N. Methods: Samples of benthic invertebrates were collected in the littoral zone of the Lago de Tota where Egeria densa was present using a handheld net method. Samples were processed in the laboratory, organisms were identified, counted and weighed. Gut contents and δ13C and δ15N stable isotopes analysis were performed as well. Results: Records of the trophic relations of aquatic invertebrates, which live in the littoral in high mountain lentic ecosystem in the Neotropics are shown. The analysis of gut contents distinguished seven food items, microphytes and MOPF were the most frequent, and through the stable isotopes analysis (δ15N) we identified four trophic levels, among resources (macrophytes and POM), and consumers (detritivores, herbivores, and predators). The two upper trophic levels concentrated greater diversity and biomass. Conclusion: Most of the organisms studied were linked to omnivorous habits, evidenced in a wide spectrum of food items in their diet. The δ15N values show an enrichment, which occurs due to the effects of the tendency towards eutrophication of the system or due to unknown values of protozoa and bacteria that plays a fundamental role in the diet of these organisms, besides the δ13C values reported in organisms consumes, allows us to suggest an affinity with native resources of the littoral zone.

Wait and snap: eastern snapping turtles (Chelydra serpentina) prey on migratory fish at road-stream crossing culverts.

There is growing evidence that culverts at road-stream crossings can increase fish density by reducing stream width and fish movement rates, making these passageways ideal predator ambush locations. In this study, we used a combination of videography and δ13C stable isotope analyses to investigate predator-prey interactions at a road-stream crossing culvert. Eastern snapping turtles (Chelydra serpentina) were found to regularly reside within the culvert to ambush migratory river herring (Alosa spp.). Resident fish species displayed avoidance of the snapping turtles, resulting in zero attempted attacks on these fish. In contrast, river herring did not display avoidance and were attacked by a snapping turtle on 79% of approaches with a 15% capture rate. Stable isotope analyses identified an apparent shift in turtle diet to consumption of river herring in turtles from culvert sites that was not observed in individuals from non-culvert sites. These findings suggest that anthropogenic barriers like culverts that are designed to allow passage may create predation opportunities by serving as a bottleneck to resident and migrant fish movement.

Dietary differences in individuals buried in a multiethnic neighborhood in Teotihuacan: stable dental isotopes from Teopancazco

This study examines the diet consumed by the population at the neighborhood center of Teopancazco, Teotihuacan, in the basin of Mexico. We used stable isotope analysis of δ13C and δ15N of the dentine collagen as well as the δ13C from enamel bioapatite from teeth (M2 and M3) of 39 of excavated burials. The results show that the diet consumed by the majority of the population during childhood and adolescence had high 13C enriched values in both dietary components: δ13C enamel_bioapatite = −2.0 ‰ ± 1.5 for the whole diet and δ13C dentine_collagen = − 9.4 ‰ ± 2.1 for the protein component. According to the bi-variable and multivariable models, some individuals consumed higher amounts of C4/CAM resources than those specified in cluster #2 (70 % C4 diet; ≥50 % C4/CAM protein). An inter-tissue adjustment of 2.3 ‰ was used to relate the enamel bioapatite data to the bone bioapatite of the models. Average δ15Ndentine_collagen was 10.2 ‰ ± 2.8 with high variability especially for four individuals, three of which belong to special burials, suggesting different access to meat or marine products due to their social or migration status and/or to pathological conditions. The data suggest that maize was the staple crop of the population not only at Teopancazco but for Teotihuacan as a whole, in the form of tortillas, tamales, atole, and protein from maize-fed animals with the inclusion of other CAM, C4, and C3 resources probably from the bountiful ecosystem of the basin of Mexico or from the wide trade system that Teotihuacan had with Mesoamerica.

River of the dammed: longitudinal changes in fish assemblages in response to dams

Although dams are a common feature on rivers throughout the world, their effects on diversity, composition, and structure of fish assemblages are often unclear. We used electrified benthic trawls and stable isotope analysis of δ13C and δ15N to determine the complex relationships between taxonomic diversity and food web structure of fish assemblages among sites in the free-flowing and impounded reaches of the Allegheny River, Pennsylvania, USA. We found higher gamma and beta fish diversity in the free-flowing section, where Brillouin diversity increased in a downstream direction; however, in the impounded section, we found decreasing diversity downstream. Analysis of similarity and non-metric multi-dimensional scaling revealed longitudinal differences in Bray–Curtis similarity between assemblages from impounded and those from free-flowing sites. Finally, using stable isotope analysis, we showed that fishes in the free-flowing section derived nutrients primarily from benthic sources while fishes in the impounded section had a stronger reliance on pelagic nutrients. Our findings reveal that dams can reduce fish taxonomic diversity, driven primarily by decreases in lotic taxa, while shifting resource use from benthic toward pelagic nutrients. A multi-faceted approach to assess the cumulative effects of dams on aquatic communities is, therefore, recommended.

Seasons of the ringed seal: pelagic open-water hyperphagy, benthic feeding over winter and spring fasting during molt

Context The ringed seal (Phoca hispida), a small phocid seal with a circumpolar Arctic distribution and a strong association with sea ice, occurs at the southern limit of its range in Hudson and James Bays: an area that experiences complete ice cover in winter and complete open water in summer. Because of the high seasonal variability in environmental conditions, it is expected that ringed seals experience seasonal changes in diet and foraging habitat, which will be reflected in body condition and biomarkers of stable isotopes and fatty acids. Aims The purpose of the present study was to investigate intra-annual variation in the feeding habits and body condition of the ringed seal. Methods Tissue samples and morphological measurements from south-eastern Hudson Bay ringed seals were obtained every month during the Inuit subsistence hunt from November 2009 to May 2011 (n = 192). Muscle samples were used for δ15N and δ13C stable isotope analysis, blubber was used for analysis of fatty acid composition, bodyweight and sculp weight were used to estimate percentage blubber, and lower right canines were used to determine age. Key results Fatty acid composition, δ15N, and δ13C varied significantly by season, suggesting seasonal changes in foraging habitat and diet. Variation in percentage blubber indicated that poorest body condition occurs following the molting and fasting period, followed by a gradual increase from late summer through fall, with the highest body condition occurring in time for freeze-up in December. Key conclusions Patterns of δ13C indicate pelagic feeding during the open-water season (August–December) when fat and energy stores are replenished, increased benthic foraging during the period of ice cover (January–May), followed by a period of fasting during the spring molt (June–July). Fatty acid composition suggested seasonal changes in diet that could include increased importance of pelagic fish in the fall during the period of positive energy balance. Implications The first continuous collection of ringed seal tissue samples provided a comprehensive seasonal pattern of biomarker composition, which is baseline data that have important applications for short-term management and ecology studies as well as long-term conservation and monitoring programs.

Gravel dredging alters diversity and structure of riverine fish assemblages

Summary1. Human activities affect fish assemblages in a variety of ways. Large‐scale and long‐term disturbances such as in‐stream dredging and mining alter habitat and hydrodynamic characteristics within rivers which can, in turn, alter fish distribution. Habitat heterogeneity is decreased as the natural riffle–pool–run sequences are lost to continuous pools and, as a consequence, lotic species are displaced by lentic species, while generalist and invasive species displace native habitat specialists. Sediment and organic detritus accumulate in deep, dredged reaches and behind dams, disrupting nutrient flow and destroying critical habitat for habitat specialist species.2. We used standard ecological metrics such as species richness and diversity, as well as stable isotope analysis of δ13C and δ15N, to quantify the differences in fish assemblages sampled by benthic trawls among dredged and undredged sites in the Allegheny River, Pennsylvania, U.S.A.3. Using mixed‐effects models, we found that total catch, species richness and diversity were negatively correlated with depth (P < 0.05), while species richness, diversity and proportion of species in lithophilic (‘rock‐loving’) reproductive guilds were lower at dredged than at undredged sites (P < 0.05).4. Principal components analysis and manova revealed that taxa such as darters in brood hider and substratum chooser reproductive guilds were predominantly associated with undredged sites along principal component axis 1 (PC1 and manovaP < 0.05), while nest spawners such as catfish and open substratum spawners including suckers were more associated with dredged sites along PC2 (P < 0.05).5. Stable isotope analysis of δ13C and δ15N revealed shifts from reliance on shallow water and benthic‐derived nutrients at undredged sites to reliance on phytoplankton and terrestrial detritus at deep‐water dredged sites. Relative trophic positions were also lower at dredged sites for many species; loss of benthic nutrient pathways associated with depth and dredging history is hypothesised.6. The combination of ecological metrics and stable isotope analysis thus shows how anthropogenic habitat loss caused by gravel dredging can decrease benthic fish abundance and diversity, and that species in substratum‐specific reproductive guilds are at particular risk. The effects of dredging also manifest by altering resource use and nutrient pathways within food webs. Management and conservation decisions should therefore consider the protection of relatively shallow areas with suitable substratum for spawning for the protection of native fishes.

STABLE ISOTOPE ANALYSIS REVEALS ANTHROPOGENIC EFFECTS ON FISH ASSEMBLAGES IN A TEMPERATE RESERVOIR

ABSTRACTEffluent from anthropogenic inputs can affect fish assemblages in aquatic ecosystems by altering species richness, diversity and trophic structure. To investigate the effects of a bleached kraft pulp mill effluent (PME) and municipal sewage treatment plant (STP) discharge on fish assemblages in a temperate reservoir, we combined standard ecological methodologies with stable isotope analysis of δ13C and δ15N. Total catch, species richness, diversity and coefficient of community loss indicated lower species richness and diversity at sites exposed to PME, whereas sewage‐exposed assemblages had intermediate richness and diversity relative to reference sites. Stable isotope analysis of δ13C and δ15N revealed that many species fed at higher relative trophic positions in the presence of both effluents and showed shifts towards increased reliance on littoral‐derived and benthic‐derived carbon at sites downstream of the PME discharge. Fishes at PME‐influenced sites had also generally higher condition factor whereas fishes at STP‐influenced sites were more variable in condition factor. These differences may, in part, be due to decreased species diversity at these sites and to nutrient enrichment manifest from the effluent discharge. While standard fisheries and ecological methodologies and stable isotope analysis have been separately used in other studies of anthropogenic effects on fishes, their integration in this study provides greater resolution than either could alone. Copyright © 2011 John Wiley & Sons, Ltd.

Little Egret (Egretta garzetta) as a bioindicator of trace element pollution in Tunisian aquatic ecosystems

The Little Egret, Egretta garzetta, has breeding colonies in the island of Chikly (in the lake of Tunis) and in Thyna saltpans (in the gulf of Gabès), two important Tunisian wetlands that are strongly affected by anthropogenic activity. Here, we used E. garzetta chick feathers for environmental monitoring of breeding grounds of this species. Since trophic ecology is fundamental when interpreting contamination levels, our approach combined both trace-element (Hg, Pb, Cd, and Se) and stable-isotope analysis of δ13C, δ15N, and δ34S. Hg, Se, and δ15N levels were higher in specimens collected on Chikly than in Thyna. These observations highlight the degree of eutrophication of the lake of Tunis. Yellow-legged gulls (Larus michahellis) breeding in the same areas also feeds in the lake and attains similar concentrations of Hg and Se. In Thyna, egrets and gulls exploit distinct foraging habitats, as demonstrated by stable isotope analysis. The highest Hg and Se concentrations were found in Thyna. This result is consistent with greater exploitation of marine resources from the gulf of Gabès.

Non-omnivorous generality promotes population stability

Using a combination of stable isotope analysis of delta13C and delta15N and long-term census data on population abundances for meiofauna in tropical aquatic rock pools, we provide evidence that species which exhibit greater variation in delta13C, an indication of a greater range of distinct carbon sources in their diet, have more stable populations than species with lower variation in delta13C. This link between increased isotope variability and reduced population variability, however, did not hold for delta15N. This suggests that increases in population stability were due to non-omnivorous feeding on multiple carbon sources within a trophic level rather than omnivorous feeding on multiple carbon sources across trophic levels. Our findings corroborate MacArthur's original hypothesis that populations that can access a greater range of resources are more stable than those which consume a more restricted range of resources.