ABSTRACT Zoophilic flies play an important role in dairy farms by transmitting diseases to livestock. These flies can cause significant damage to animal husbandry through physical disturbance and the spread of pathogens. This study aims to identify insect species in both traditional and industrial animal husbandry settings, focusing on the dominant species of zoophilic flies. The research involved collecting livestock insects from March to October, between 2017 and 2021, using insect nets, sticky nets, and light traps. A total of 10,942 flies were collected from livestock farms in three counties Marvdasht, Shiraz, and Zarghan. The collected flies belong to 21 species from 6 families. Musca domestica, Fannia canicularis, Musca autumnalis, Stomoxys calcitrans, Muscina stabulans, Calliphora vomitoria, and Lucilia cuprina were identified as the dominant species. Musca domestica, F. canicularis and M. autumnalis accounted for 70.24% of the total number of flies on livestock farms. Among the collected samples, Eristalis tenax (Syrphidae) was found with a frequency of 0.96%. The importance of this species is related to the presence of its larvae that cause myiasis in livestock. Identification of the dominant species of flies put the scientific basis to reduce their populations in dairy farms and the costs caused by the damage made by these pests to livestock farms through safe ways, such as biological control.