Aggregate Stability Research Articles

Enhancing soil ecosystem multifunctionality through combined conservation tillage and legume-based crop rotation in the North China Plain

Conservation agriculture (CA), based on principles of conservation tillage (CT) and crop rotations, has been adopted as a solution to global climate change. However, interactions between these principles and their cumulative effects on soil functions and crop productivity are not yet fully understood. Herein, a 4-year filed experiment was conducted to assess the impact of CA on soil ecosystem multifunctionality (EMF) in the North China Plain (NCP). The results showed that CA improved EMF by up to 532 % compared to traditional agriculture (rotary tillage under wheat and maize rotation system). This enhancement is mainly driven by a 12.3 % increase in soil organic carbon (SOC) storage, an 8.3 % reduction in soil carbon to nitrogen ratio (C: N), a 68.3 % boost in soil enzyme activities index (SEI), and a 59.7 % increase in available phosphorus (AP) under legume-based crop rotations (LBCR) compared to maize-wheat-maize-wheat (MWMW). The principle of CT improved soil physical structure, enhancing soil aggregate stability by up to 38.1 % compared to rotary tillage (RT). Although, the benefits of CT on crop yield were not always observed, positive interactions on crop yield occurred under LBCR combined with CT. For instance, the soybean-wheat-soybean-wheat (SWSW) rotation produced 40.8 % higher yields than the MWMW rotation under CT. Overall, benefits of CT in improving soil structure, along with the increased diversity crop residues, adjustments in soil nutrient stoichiometric ratios, and enhanced soil enzyme activity under LBCR, led to improved SOC sequestration, crop yield and EMF under CA. The positive interactions between the principles of CA demonstrate its ability to enhance ecosystem multifunctionality. As a result, the combination of CT and LBCR within CA is recommended to sustain the productivity in NCP and other regions with similar conditions.

Comparative Study of pH-Responsive and Aggregation Stability of Bosutinib-Loaded Nanogels Comprising Gelatin Methacryloyl, Carboxymethyl Dextran, and Hyaluronic Acid for Controlled Drug Delivery in Colorectal Cancer: An Extensive In Vitro Investigation.

This study investigates the use of pH-responsive nanogels for delivering Bosutinib (BOSU) in colon cancer treatment. Nanogels were formulated using three polymers: hyaluronic acid (HA), carboxymethyl dextran (CMD), and gelatin methacryloyl (GelMA). These nanogels achieved high drug entrapment efficiencies (80-90%) through polymer mixing with BOSU, followed by EDC/NHS cross-linking and sonication. The nanogels were stable, with negative zeta potentials (-20 to -30 mV) and particle sizes between 100 and 200 nm. Fourier-transform infrared analysis confirmed successful methacrylation in GelMA nanogels. Sustained BOSU release at pH 5.0 was observed, resembling tumor environments, compared to slower release at normal pH (7.4). Cytotoxicity tests showed 70-80% cell survival reduction in HCT116 colon cancer cells at higher doses, and GelMA-BOSU nanogels notably reduced cell migration. Antiangiogenic effects were confirmed in a chick chorioallantoic membrane model, highlighting the potential of these nanogels for targeted BOSU delivery in colon cancer therapy.

Changes in soil mechanical and hydraulic properties through regenerative cultivation measures in long-term and farm experiments in Germany

Regenerative agriculture has been associated with improved soil structure and soil fertility. However, conclusive evidence of its efficacy has remained elusive owing to a lack of long-term experimental studies. In this study, we assessed the impact of diverse regenerative agricultural measures on soil mechanical and hydraulic properties and indicators. Tested treatment factors included reduced tillage versus plowing, along with different levels of compost, mulch, and the application of ferments and compost tea. We measured in situ soil strength via soil penetration (from 0 to 0.8 m depth) and shear resistance (at 0.08 and 0.23 m depth) and assessed field-saturated hydraulic conductivity and ex situ soil aggregate stability (at 0.07 and 0.23 m depth). The experiments were conducted at five sites in Hesse, Germany, including one organic long-term experiment (LTE, since 2010) in Neu-Eichenberg and three organic and one conventional on-farm experiments to cover different soil types, weather conditions, and field practices. The soil types are classified as Luvisol and Vertic Cambisols, and the soil texture ranges from silt loam to silty clay loam. In the LTE, significant differences in aggregate stability and shear resistance were noted between treatments, with a higher geometric mean aggregate diameter at 0.07 m depth in 2021 and 2022 and a higher shear resistance at 0.19 m and 0.23 m in 2020 and in 2021, respectively, in the reduced tillage systems. However, no significant differences were observed among treatments for field-saturated hydraulic conductivity, which was overall very high, showing that reduced tillage did not negatively influence saturated infiltration, albeit bulk density is higher than in the conventionally plowed system. The soil penetration resistance was generally higher for the reduced tillage treatments across depths of 0.0–0.30 m, albeit not statistically significant (p > 0.05). Significantly higher water-stable aggregates and geometric mean diameters were observed for regenerative agricultural treatments in three of the on-farm experiments at a depth of 0.07 m. The shear resistance was significantly higher in regenerative agriculture units in specific years and depths. Although the outcomes are encouraging, the variability of the effects of reduced tillage and organic amendments in affecting soil properties highlights the need for further long-term research including farm trials. This is essential to fully understand the effects of regenerative practices on soil physical quality.

Role of water-repellent characteristics in organic amendments on water stability of soil aggregates and carbon retention: a laboratory approach

Role of water-repellent characteristics in organic amendments on water stability of soil aggregates and carbon retention: a laboratory approach

Coordination-Induced Magnetism Strategy for Highly Selective and Efficient Uranium Separation.

Highly efficient separation of dispersed uranium is important for the sustainable development of nuclear industry, and adsorption is the most recognized approach. However, there are many coexisting interfering metal ions that compete with uranyl ion for the chelating ligands in the adsorbents and lead to low separation selectivity and efficiency. Herein, a coordination-induced magnetism strategy is presented for the separation of uranium based on the conversion of diamagnetic cyanoferrocene (Fc-CN) nanocrystals to uranium-containing magnetic recoverable ferromagnetic aggregates. Different from previous adsorption strategies, this strategy combines the mechanisms of photocatalytic uranium enrichment and chemical uranium adsorption. Under light irradiation, electron of Fe(II) in Fc-CN is excited and transfers to uranyl ion via the cyano group to form tight coordination bond between N atom in cyano group and uranium. This phenomenon is unique for uranyl ion, and thus, a high uranium removal rate of 97.98% is achieved in simulated nuclear wastewater with the presence of tremendous interfering ions, proving its highly selective and efficient uranium separation performance. The ability to form highly stable magnetic aggregates via photoinduced interaction between Fc-CN and uranium enriches the understanding on the chemical properties of Fc-CN and uranium.

Sugarcane bagasse derived biochar potential to improve soil structure and water availability in texturally different soils

Low organic matter content is one of the main constraints in arid and semiarid regions. This constraint and its negative influences on soils and plant growth may be alleviated by biochar (BC). Furthermore, improving soil physical and hydraulic attributes by application of biochar has received increased attention. Therefore, in the present study, the effects of sugarcane bagasse-derived biochar on the structural stability, water availability, and pore-size distribution (PSD) of three texturally different calcareous soils collected from different agro-climatologically regions were examined during a long-term experiment. Low and high-temperature biochars, produced in a muffle furnace by the traditional slow pyrolysis method at 300 °C (BC300) and 600 °C (BC600) were evaluated. Pots (15 kg) were filled with three different silty-clay Inceptisols (SCInc), silty-clay-loam Alfisols (SCLAlf), and loam Aridisols (LArid) soils mixed with 0 (control), 1, 2, and 3 w/w% of BC300 and BC600 during 540 days of incubation. The high energy moisture characteristic (HEMC) data was modeled using a modified van Genuchten function to quantify aggregate stability through stability ratio (SR) and structural stability index (SSI). The plant available water (PAW), least limiting water range (LLWR), and integral water capacity (IWC) were calculated with two matric suctions (h) of 330 cm for field capacity (FC) and 15,000 cm for permanent wilting point (PWP). Then the integral energy (EI) values were calculated (EIIWC). Results indicated that the incorporation of 3 w/w% biochar significantly (p < 0.01) increased SR (35 to 100%) and SSI (21 to 28%) indices in all three soils. Biochar significantly increased modal suction (MS) in LArid soils (5 to 158%); whereas, decreased MS of the other soils (3 to 43%). MS, SR, and SSI of BC300 and BC600-treated soils were not significantly different. PAW, LLWR, and IWC significantly decreased in the SCInc (18 to 61%, 8 to 44%, and 6 to 35%) and SCLAlf (8 to 44%, 18 to 35%, and 20 to 47%) soils and increased in LArid (4 to 54%, 3 to 61%, and 24 to 111%) soil with increasing biochar doses. There were no changes in EIIWC in biochar-treated LArid soil where PAW, LLWR, and IWC increased. Biochar increased EIIWC across the studied soil from 1% to 3.38 folds, thereby increasing the gradient of water potential to absorb the available water. Soil and soil-biochar mixtures exhibited heterogeneous and multimodal pore-size distribution (PSD). Biochar promoted the PSD peaks related to water-transmitting pores in SCInc and SCLAlf soils while decreased in LArid soil. In conclusion, results indicated that among the applied levels of biochar, the application of 3 w/w% biochar is suggested as a suitable way to improve soil physical behavior and structural stability.

Long-term rice cultivation enhances root development and yields by improving the structural properties of soil aggregates in saline–alkaline environments

Rice cultivation has the ability to improve saline soils. However, the effects of long-term rice cultivation on saline soil chemistry, salt ions, root characteristics, and aggregate formation remain unclear. In this study, the impact of different planting durations (1, 3, 5, 8, and 10 years) on the spatial and temporal transport of soil salts, soil chemical properties, distribution and stability of aggregates, root characteristics, and yields, were assessed. Long-term rice cultivation reduced soil pH, exchangeable sodium percentage (ESP), and EC in the 0-60cm soil layer compared to 1Y (5.45%, 61.57%, and 81.87% reduction in topsoil (0-20cm) and 8.54%, 72.82%, and 71.94 in subsoil (20-60cm), respectively). However, the soil organic matter (OM), alkaline nitrogen (AN), phosphorus (AP), and potassium (AK) increased (217.09%, 90.18%, 89.23%, and 179.18% increase in topsoil and 223.72%, 81.24%, 174.28%, and 172.57% increase in subsoil, respectively). Additionally, the Cl-, K+, and Na+ in the soil gradually leached downward with the increase in the duration of rice cultivation (55.87%、36.08%、59.80%). Simultaneously, the increased duration markedly elevated the soil macroaggregate content (11.43%-20.04% by dry-sieving and 53.48%-288.09% by wet-sieving), clayey grain content (59.17%-198.33%), and soil aggregate stability, especially for 8Y and 10Y. The improved soil structure and root characteristic under long-term rice cultivation conditions increased the yield (28.89-54.81%). Partial least squares path model displayed that rice cultivation affected the aggregate stability, root characteristics, and rice yield via the corresponding chemical properties (pH, EC, and ESP), nutrient contents (AN, AP, AK, and SOM), and salt ion contents (Cl-, K+, and Na+). These findings are critical to understanding aggregate formation in saline soils.

Capping agent-free aqueous gold nanoparticles generated by an environmentally friendly plasma-liquid method

This study investigates a simple approach for synthesizing gold nanoparticles (Au NPs) using atmospheric pressure plasma without the need for reducing agents, additives, or capping agents. The size, morphology, optical properties, and aggregation stability of the synthesized Au NPs were characterized using scanning electron microscopy, UV-vis spectroscopy, and zeta potential analysis. The UV-vis absorption spectra of the gold nanoparticles, obtained at various Au3+ concentrations and treatment durations with anode plasma discharge, were analyzed. The UV-vis spectra revealed a surface plasmon resonance absorption band between 530–600 nm, indicative of Au NP formation. Results demonstrated that the size of Au NPs can be tuned within the range of 30–75 nm by adjusting the gold salt precursor concentration from 0.3 to 2.5 mmol/L. It was found that plasma treatment for 3–10 minutes, at different Au3+ concentrations, resulted in the formation of Au NPs with varying sizes and morphologies. The study showed that particles with different shapes – spherical, hexagonal, and triangular – were formed depending on the initial concentration of Au3+. The plasma-chemically synthesized nanoparticles at an Au3+ concentration of 0.06 mmol/L were predominantly spherical and exhibited the highest stability (lasting 12–24 hours), with a zeta potential of –20 to –22 mV, compared to samples with Au3+ concentrations ranging from 0.3 to 2.5 mmol/L.

Effects of Applying Organic Amendments on Soil Aggregate Structure and Tomato Yield in Facility Agriculture

Amendment significantly improves soil structure and promotes crop growth. To combat soil degradation and low crop yields in facility agriculture, it is crucial to study the optimal application rate of amendments. This study analyzed the effects of biochar, vermicompost, and mineral-source potassium fulvic acid on the stability of aggregate structure, soil nutrient content, and tomato yield in cambisols, providing a theoretical basis for improving the soil quality of plastic greenhouses in Southern China. A pot experiment on tomato cultivation was carried out in yellow-brown soil in plastic greenhouses. The experiment included eight treatments: 1% biochar (B1); 3% biochar (B3); 5% biochar (B5); 3% vermicompost (V3); 5% vermicompost (V5); 0.1% mineral-source potassium fulvic acid (F1); 0.2% mineral-source potassium fulvic acid (F2); and the control condition without adding soil amendments (CK). The results showed that the biochar and vermicompost treatments effectively reduced soil bulk density and increased total soil porosity. Compared to the control, treatments with soil amendments significantly increased soil pH and had different effects on soil nutrients: F2 showed the most significant improvement in the content of available nitrogen, available phosphorus, and available potassium, with an increase of 133.33%, 834.59%, and 74.34%, respectively; B3 treatment had the highest increase in dissolved organic carbon (DOC), while B5 treatment had the highest organic matter content. Compared to the CK, the particle size of the biochar treatment was mainly 0.053~0.25 mm, while the V3, F1, and F2 mainly occurred with a particle size &gt; 0.25 mm; and V3 has the best aggregate stability. Biochar, vermicompost, and mineral potassium fulvic acid can all promote tomato yield, with the F2 and V3 treatments having a yield increase effect of over 30%. Furthermore, Pearson’s correlation analysis showed a highly significant positive correlation between geometric mean diameter (GMD) and mean weight diameter (MWD), water-stable macroaggregate content (R0.25), and a positive correlation between alkaline-dissolved nitrogen, available phosphorus, dissolved organic carbon content, and aggregate stability indicators. Adding 0.2% mineral-source potassium fulvic acid optimizes cambisols’ properties, enhances aggregate formation and stability, boosts tomato yield, and shows great application potential.

Saline Water Irrigation Changed the Stability of Soil Aggregates and Crop Yields in a Winter Wheat–Summer Maize Rotation System

Irrigation using saline water is extensively used in areas of agricultural production where freshwater is scarce. However, saline water irrigation adversely impacts soil’s physicochemical characteristics and crop productivity. In this study, we established irrigation water with five salinity levels (ECiw, 1.3, 3.4, 7.1, 10.6, 14.1 dS·m−1) to investigate how these salinity levels influenced grain yields as well as soil salinity, alkalinity, sodicity, and aggregate stability in the 0~20 cm soil layer of a wheat and maize rotation field (in 2022–2023). Tukey’s test, entropy-weighted TOPSIS, and the least squares method were used to analyze the significance analysis, comprehensively evaluate the soil aggregate stability and soil index comprehensive score (SICS), and achieve linear fitting, respectively. The results showed that when ECiw &gt; 3.4 dS·m−1, there was a significant increase in the soil salinity, pH, and sodium adsorption ratio. When ECiw &gt; 7.1 dS·m−1, a significant reduction in soil aggregate stability was observed. When ECiw ≤ 3.4 dS·m−1, there was no significant reduction in the grain yields of wheat and maize. Furthermore, the annual grain yields of wheat and maize decreased by 5% and 10%, respectively, resulting in a change in ECiw values from 2.98 to 4.24 dS·m−1, based on the linear regression analysis of SICS and ECiw, as well as the annual grain yields and SICS. Under uniform irrigation conditions, the soil salinity, alkalinity, and sodicity were lower, and soil aggregate indexes were more stable at the maturity stage of maize.

Rill Erosion Due to Wildfire or Deforestation in Forestlands of Northern Iran

Rill erosion, mostly affecting steep and long hillslopes, is one of the most severe effects of deforestation and wildfires in natural ecosystems. Specific monitoring and accurate but simple models are needed to assess the impacts of these forest disturbances on the rill detachment process. To address this need, this study has simulated the rill detachment capacity (Dc) through flume experiments on samples of soils collected in hillslopes after deforestation and severe burning. The associations between Dc and organic matter (OM) and the aggregate stability of soil (WSA), two key parameters influencing the rill detachment process, have also been explored under the two soil conditions (deforested and burned soils) using multivariate statistical techniques. Finally, linear regression models to predict Dc from these soil parameters or the hydraulic and morphological variables (water flow rate, WFR, and soil slope, S), set in the flume experiments, have been proposed for both soil conditions. Higher Dc in samples from deforested sites compared to the burned soils (+35%) was measured. This Dc increase was associated with parallel decreases in OM (−15%) and WSA (−34%) after deforestation compared to the wildfire-affected sites. However, the discrimination in those soil properties between the two soil conditions was not sharp. Accurate linear equations (r2 &gt; 0.76) interpolating Dc and the shear stress (τ) have been set to estimate the rill erodibility (Kr) to evaluate soil resistance in erosion models to be applied in deforested or burned sites.

Grass species and mycorrhizal fungi improved aggregate stability of compacted and vegetated soils

Abstract Aim Compaction of slope soils can substantially hinder root penetration of grass cover, which may be alleviated through the colonisation of arbuscular mycorrhizal (AM) fungi and aggregate stabilisation. We investigated aggregate stabilisation and breakdown mechanisms in compacted dense mycorrhizal soils. Methods A pot-culture experiment with seven treatments (five replicates per treatment) was implemented. In a local decomposed granitic soil, we inoculated two grass species (Chrysopogon ziaanioides and Cynodon dactylon) with AM fungi. We used loose soil to grow C. dactylon to compare it with compacted dense soil, as well as pots without a plant and/or fungal inoculation for comparison. After 20 weeks of cultivation, we measured root and AM fungal characteristics, soil organic matter and aggregate properties by dry sieving, wet sieving and Le Bissonnais methods. Results Compaction led to the formation of macro-aggregates (&gt; 0.25 mm) but had a negative influence on the aggregate stability. The fungal inoculation increased polysaccharide production and aggregate stability in the compacted soil vegetated with C. dactylon. The inoculated C. ziaanioides showed a similar level of aggregate stability as the inoculated C. dactylon, but the uninoculated group demonstrated higher aggregate stability compared with the inoculated group owing to root decomposition. The aggregate stability against various breakdown mechanisms was related to the established aggregate hierarchy and qualitative organic matter inputs. Conclusion Soil organic matter supplied by grass species together with the mediation of AM fungal hyphae played a crucial role in the systemic enhancement of aggregate stability in the compacted soil.

Synergy Between Woody Peat and Bentonite Alters Stability of Soil Organic Carbon in Coarse Soil by Enhancing Capacity for Soil Aggregation and Hydro‐Physical Properties

ABSTRACTCoarse soil has a poor structure and is susceptible to wind and water erosion, thereby making it difficult to maintain the soil organic carbon (SOC) content. Woody peat (WP) is an organic material that can increase the SOC content of the soil, while clay materials can rapidly enhance the capacity for soil aggregate formation. In order to explore the synergistic effects of WP and clay materials (bentonite and red clay) on the aggregate structure and hydro‐physical properties of coarse soil, as well as the mechanism associated with SOC mineralization (ΔSOC), we conducted an incubation study using lou soil (L0) and loessial soil (H0) with three treatments: addition of WP alone (LW, HW), mixture of WP and bentonite (LWB, HWB), and mixture of WP and red clay (LWR, HWR). The three treatments enhanced the proportion of macroaggregate (&gt; 2 mm) and aggregate stability of the two soils, and optimized the water retention and ventilation performance. The highest aggregate stability of LWB and HWB can be attributed to the positive synergistic effect of WP and bentonite, and bentonite was more effective than red clay due to its crystal structure. The results also showed that the ΔSOC values were significantly lower under LWB and HWB than those under WP addition alone and adding the mixture of WP and red clay (p &lt; 0.05). Moreover, partial least squares path modeling analysis showed that the hydro‐physical properties of the two improved soils inhibited SOC mineralization (p &gt; 0.05), whereas particulate organic carbon (POC) content significantly accelerated SOC mineralization (p &lt; 0.01). The synergistic effect of clay materials increased mineral‐associated organic carbon (MAOC), which was beneficial to maintain the long‐term effectiveness of WP. Overall, our results demonstrated that the synergistic use of WP and bentonite enhanced the aggregate stability and hydro‐physical properties of coarse soil and improved SOC storage capacity. These results provide scientific and theoretical guidance to facilitate the rapid improvement of coarse soil through engineering measures in arid and semi‐arid areas with water and fertilizer limitations.

Development of a demulsifier and technological process study for the preparation of highly viscous heavy oils for refinement

One of the challenges in the petroleum industry is the production of highly viscous heavy oils, often accompanied by undesirable emulsion formation with very high aggregate stability. To prepare the crude oils for refinement, the oils must be destroyed. The destruction of aqueous emulsions containing highly viscous heavy oils is an intricate task owing to the comparatively small disparity in water and oil densities, as well as the high viscosity of the dispersive medium and its high content of mechanical impurities. The problem of the destruction of petroleum emulsions arises desperately during the desalination and dehydration of crude oils with a high content of emulsifiers (resins, asphalts, paraffin, etc.) The creation of a highly efficient demulsifier and the development of an optimal technological process for the preparation of highly emulsifying oils that are distinguished by the composition and quantity of emulsifiers of West Siberian oils, which are widely refined in Russia, are the ways to resolve this challenging and timely issue. This study focuses on the viscous heavy oil of the Verbliouje deposit in the Astrakhan region. It also explains the creation of an effective demulsifier for the destruction of petroleum emulsions characterized by a very high emulsifying power and the fundamental principles of the development of deep dehydration technology and desalination of such high-viscosity heavy oils.

Understanding the effect of cropping system on soil health at the Northwestern Ontario Agricultural Research Station in Canada

Anthropogenic activities impact soil in varying degrees, from preserving natural landscapes to intensive agriculture which among the farm practices that impact the soil are the cropping systems. Information on cropping systems and soil impacts in northern territories is still missing. This study assesses the effect of different cropping systems on soil health -physical, chemical and biological soil properties and indicators of soil health - at the Lakehead Agricultural Research Station [LUARS] in northern Ontario, Canada. The study compares three cropping systems (perennial crops-pasture, grass, and annual crops -wheat, barley, corn, soybeans) and two forest areas (conifer plantation and naturally regenerating mixed wood forest) at LUARS. Soil samples were collected at different depths and analyzed for various indicators using the Cornell Soil Health Assessment framework. The results showed the soil health scores varied among cropping systems, with natural forest and perennial crops-pasture having higher scores compared to annual crops -wheat, barley, corn, soybeans. Soil organic matter was found to be lowest in annual crops -wheat, barley, corn, soybeans, while aggregate stability was highest in natural forests. The study also identifies the soil health gap, which represents the difference between the health of a particular cropping system and a benchmark. The soil health gap analysis can help farmers implement practices to improve soil health and increase the resilience and sustainability of agroecosystems. Overall, this study emphasizes the importance of understanding the effect of cropping systems on soil health and provides insights into potential strategies for improving farm practices.

Insulin Stabilization Designs for Enhanced Therapeutic Efficacy and Accessibility.

ConspectusInsulin has remained indispensable in the treatment of diabetes since it was first discovered in 1921. Unlike small molecular drugs, insulin and other protein drugs are prone to degradation when exposed to elevated temperatures, mechanical agitation during transportation, and prolonged storage periods. Therefore, strict cold-chain management is crucial for the insulin supply, requiring significant resources, which can limit the access to insulin, particularly in low-income areas. Moreover, although insulin formulations have advanced tremendously in the last century, insulin treatment still imposes a challenging regimen and provides suboptimal outcomes for the majority of patients. There is an increasing focus on pursuing improved pharmacology, specifically on safer, more user-friendly insulin therapies that minimize the self-management burden. These challenges underscore the need for developing novel insulin formulations with improved stability that are compatible with advanced insulin therapy.Insulin stabilization can be achieved through either chemical modification of insulin or formulation component design. Inspired by insulin-like peptides from invertebrates, we have developed novel stable insulin analogs based on a fundamental understanding of the insulin receptor engagement for insulin bioactivity. We created a novel four-disulfide insulin analog with high aggregation stability and potency by introducing a fourth disulfide bond between a C-terminal extended insulin A-chain and residues near the C-terminus of the B-chain. In an effort to stabilize insulin in its monomeric state to develop ultrafast-acting insulin with rapid absorption upon injection, we have developed a series of structurally miniaturized yet fully active insulin analogs that do not form dimers due to the lack of the canonical B-chain C-terminal octapeptide. Additionally, our study provided strategies for expanding the scope of cucurbit[7]uril (CB[7])-assisted insulin stabilization by engineering safe and biodegradable CB[7]-zwitterionic polypeptide excipients. We also explored insulin N-terminal substitution methods to achieve pH-dependent insulin stabilization without prolonging the duration of action.This Account describes our exploration of engineering stable insulin analogs and formulation design strategies for stabilizing insulin in aqueous solutions. Beyond conventional stabilization strategies for insulin injections, the unmet challenges and recent innovations in insulin stabilization are discussed, addressing the growing demand for alternative, less invasive routes of insulin administration. Additionally, we aim to provide a thorough overview of insulin stabilization from the perspective of commercially available insulin drugs and common pharmaceutical engineering practices in the industry. We also highlight unresolved insulin stabilization challenges and ongoing research strategies. We anticipate that further emphasis on collective efforts of protein engineering, pharmaceutical formulation design, and drug delivery will inform the development of stable and advanced insulin therapy.

Evaluating the Effectiveness of the Biodegradable Superabsorbent Polymer (Fasal Amrit) on Soil Hydrological Properties: A Laboratory Rainfall Simulation Study

Superabsorbent polymers (SAPs) are effective soil amendments that can control soil erosion by improving soil quality. However, many commercial SAPs face challenges including limited biodegradability, high costs, and adverse effects on soil hydrological properties, which can lead to increased water and soil loss. This study examined the potential of lower dosages of biodegradable SAPs to improve the hydrological properties of “Shimajiri-maji” (clay) soil. Three concentrations of biodegradable Fasal Amrit polymer (EFP) (P1: 0, P2: 3 g m−2, and P3: 6 g m−2) were evaluated under three simulated rainfall intensities (I1: 35; I2: 70 and I3: 110 mm h−1) and two gradients (7.5%, and 15%) during consecutive storms. The time to generate runoff, infiltration, runoff, soil loss, and water storage (WS) were quantified over one hour. The results show that runoff generation was delayed in EFP-treated soils compared to the control. Both polymer treatments enhanced infiltration (P2 &gt; P3 &gt; P1) and reduced runoff and soil loss (P2 &lt; P3 &lt; P1). Higher EFP rates improved water storage at surface depths (P3 &gt; P2 &gt; P1). EFP-treated soils exhibited lower interrill erodibility, suggesting greater resistance to soil erosion compared to the control. EFP treatments also significantly improved the soil’s physical properties (bulk density, porosity, organic matter, aggregate stability). EFPs can diminish runoff and soil loss as the EFP-treated plots exhibited greater aggregate stability than the control. It was concluded that low EFP concentrations can improve soil hydrological properties and mitigate soil erosion. Further investigations are needed to optimize the EFP concentrations for different soil types.

Mulching Improves the Soil Hydrothermal Environment, Soil Aggregate Content, and Potato Yield in Dry Farmland

Mulching could effectively improve the soil hydrothermal environment, improve changes in the soil structure, increase entropy, and conserve soil moisture to solve the problem of grain reduction caused by perennial drought in Northwest China. Thus, a two-growing-season field experiment (2020–2021) with five treatments (PM1, biodegradable plastic film mulching; PM2, plastic film mulching; SM1, straw strip mulching; SM2, crushed corn straw full mulching; and CK, no mulching as the control) was conducted to investigate the effects of different mulching materials on the soil hydrothermal environment, soil aggregate distribution, stability, and tuber yield of rainfed potato farmland in Northwest China. Over two growing seasons, mulching planting, on average, increased (p &lt; 0.05) the soil moisture at the 0–200 cm depth by 9.0% relative to CK (SM2 (11.6%) &gt; SM1 (10.3%) &gt; PM2 (8.6%) &gt; PM1 (7.0%)). The mulching treatments significantly regulated the soil temperature during the whole growth period, in which plastic mulching significantly increased the soil temperature of the 0–25 cm soil depth during the whole growth period by 2.1 °C (PM2 (2.1 °C) &gt; PM1 (2.0 °C)); meanwhile, straw mulching significantly reduced the soil temperature by 1.4 °C (SM2 (0.9 °C) &gt; SM1 (0.6 °C)). All mulching treatments improved the soil macroaggregate content and soil aggregate stability in all soil depths from 0 to 40 cm, with increases of 31.4% and 27.1% in the mean weight diameter (MWD) and 22.6% and 21.2% in the geometric mean diameter (GWD) compared with CK, respectively. Straw and plastic mulching significantly increased the fresh tuber yield by 12.5% and 12.6% compared with CK, respectively. The increases were greatest in SM2 and PM2. Crushed corn straw full mulching is difficult to sow and harvest; therefore, straw strip mulching could improve the soil hydrothermal environment, increase production, and provide an environmentally friendly technology for dryland potato production.

Collective self-caging of active filaments in virtual confinement

Motility coupled to responsive behavior is essential for many microorganisms to seek and establish appropriate habitats. One of the simplest possible responses, reversing the direction of motion, is believed to enable filamentous cyanobacteria to form stable aggregates or accumulate in suitable light conditions. Here, we demonstrate that filamentous morphology in combination with responding to light gradients by reversals has consequences far beyond simple accumulation: Entangled aggregates form at the boundaries of illuminated regions, harnessing the boundary to establish local order. We explore how the light pattern, in particular its boundary curvature, impacts aggregation. A minimal mechanistic model of active flexible filaments resembles the experimental findings, thereby revealing the emergent and generic character of these structures. This phenomenon may enable elongated microorganisms to generate adaptive colony architectures in limited habitats or guide the assembly of biomimetic fibrous materials.

How different soil surface treatments in urban areas affect soil pore structure and associated soil properties and processes

Soil water and temperature regimes in urban environments are greatly affected by different surface treatments, and these may lead even to changes in soil properties. The goal of this study was to find out how soil properties had changed after 8 years of soil surface modification. Five surface treatment scenarios were considered: bare soil (BS), bark chips (BC), concrete paving (CP), mown grass (MG), and unmown grass (UG). X-ray computed tomography and micromorphological analyses showed that character of pores in soils with grass (MG, UG), which were mainly influenced by organisms living in soils and roots, differed from pore character in soil covers BC or CP, which mostly were impacted by organisms living in soils. Both groups differed from BS, which was predominantly affected by the regular treatment consisting in weed removal and soil loosening. Soil under BC was more compact than for other treatments due to decomposition of the bark chips mulch and migration of mulch components. Organic matter content was greatest but its quality lowest in the BC soil, followed by UG, MG, CP, and BS. The highest aggregate stability assessed using the water-stable aggregates (WSA) index was found for UG, followed by MG, BC, CP, and BS. The greatest water retention ability was observed for BC followed by UG, MG, CP, and BS. The unsaturated hydraulic conductivities for pressure head of –2 cm measured for UG, MG, and BS were much higher than were those for CP and BC. Finally, the greatest net CO2 efflux was measured for BC and MG, followed by UG, CP, and BS. CO2 emission correlated negatively with soil physical quality expressed as slope of the soil water retention curve at its inflection point. In general, the best soil conditions were observed for UG. No treatment considerably aggravated soil condition.