AbstractHigh temperature stress during the reproductive growth stage of wheat (Triticum aestivum L.) can cause extensive yield losses. As the plant hormone auxin is a key regulator of reproductive development, we studied the effect of auxins on grain yield in five wheat lines exposed to moderate heat stress (34–35°C) for 6 h per day for 6 days during early flowering (booting stage to anthesis). ‘CDC Go’, a semi‐dwarf (Rht‐B1b) cultivar, responded to auxin application (1 µM) by producing higher grain number and yield under control and heat stress conditions. The effect of five different auxins on grain yield in ‘CDC Go’ was dependent on spike developmental stage at application and position within the spike, with 4‐Cl‐IAA at 1 µM being the most effective auxin treatment. The presence of Rht dwarfing mutant alleles of Rht‐B1 and Rht‐D1 alone did not increase auxin‐induced grain yield when tested in lines isogenic for these alleles. In the field, 4‐Cl‐IAA (1 µM) increased grain yield by 6%–8% only in ‘CDC Go’, one of six hard red spring wheat cultivars tested over two growing seasons in the western Canadian prairies. When 4‐Cl‐IAA application increased grain yield and number, the grain protein content was not affected; when it maintained grain yield in plants with lower biomass, grain protein content was reduced. Our field results suggested that both genotype and environment affect auxin‐induced enhancement of wheat grain yield. We recommend testing target environments with heat stress as a focus of a breeding programme along with further testing of auxin as a crop enhancement tool.