Spring Beauty Research Articles

Broadscale Assessment of Groundhog (Marmota monax) Predictions of Spring Onset No Better than Chance

AbstractGroundhog Day is a widespread North American ritual that marks the onset of spring, with festivities centered around animals that humans believe have abilities to make seasonal predictions. Yet, the collective success of groundhog Marmota monax prognosticators has never been rigorously tested. Here, we propose the local climate-predicted phenology of early blooming spring plants (Carolina spring beauty, or Claytonia caroliniana, which overlaps in native range with groundhogs) as a novel and relevant descriptor of spring onset that can be applied comparatively across a broad geographical range. Of 530 unique groundhog-year predictions across 33 different locations, spring onset was correctly predicted by groundhogs exactly 50% of the time. While no singular groundhog predicted the timing of spring with any statistical significance, there were a handful of groundhogs with notable records of both successful and unsuccessful predictions: Essex Ed (Essex, Connecticut), Stonewall Jackson (Wantage, New Jersey), and Chuckles (Manchester, Connecticut) correctly predicted spring onset over 70% of the time. By contrast, Buckeye Chuck (Marion, Ohio), Dunkirk Dave (Dunkirk, New York), and Holland Huckleberry (Holland, Ohio) made incorrect predictions over 70% of the time. The two most widely recognized and long-tenured groundhogs in their respective countries—Wiarton Willie (Canada) and Punxsutawney Phil (United States)—had success rates of 54% and 52%, respectively, despite over 150 collective guesses. Using a novel phenological indicator of spring, this study determined, without a shadow of a doubt, that groundhog prognosticating abilities for the arrival of spring are no better than chance.

The seasonal development of Crocus in the Mari El Republic conditions

Crocuses are beautifully flowering corm-bearing plants. They begin the growing season immediately after the snow melts. The research contains the results of phenological observations of 2014-2019 for plants of 6 Crocus taxa in the conditions of the Mari El Republic. Cultivars with early, medium and late periods of phenophase passage were identified. A flowering phenospectrum of the studied crocuses has been compiled. Crocuses vegetate from mid-April to early June for 40-50 days, and bloom from the third decade of April to early May. C. chrysanthus 'Prince Cloues' is characterized by the earliest unfolding of leaves and a short duration of their growth. The greatest value for landscaping is C. versicolor, characterized by the longest growing season and the earliest flowering, as well as C. chrysanthus ' Romance’ and C. angustifolia, characterized by the longest flowering within 2 weeks. The flowering of C. sieberi 'Spring Beauty’ is unstable. The obtained data on the seasonal development of crocuses can be used when including them in rockeries, alpine slides and other flower beds in natural-style gardens.

Self-Fertilization and Herbivory in a Rare Alpine Plant in California, Claytonia megarhiza (Montiaceae)

Reproduction in alpine habitats is challenging because of the short growing season, low temperatures, and high winds. This predicts alternative strategies for sexual reproduction in plants: compensatory measures such as larger floral displays and greater floral longevity to attract scarce pollinators and maintain outcrossing, or high levels of autonomous self-fertilization to assure reproduction in the absence of reliable pollinators. Here, we assessed the roles of animals (crawling insects, flying insects, and vertebrates) on the reproductive success of Claytonia megarhiza (A. Gray) S. Watson (alpine spring beauty). We measured fruit set and leaf herbivory while excluding animals from individual plants at a single site in Yosemite National Park, California. We found that plants were capable of setting fruit in the absence of pollinators and that, in the presence of animals, there was a 42% reduction in fruit set and a 159% increase in leaf damage. This suggests that Claytonia megarhiza may reproduce primarily by self-fertilization, and that herbivory may limit the reproductive success of this species near its southern range edge in California.

Responses of insect pollinators and understory plants to silviculture in northern hardwood forests

Communities of flower flies (Diptera: Syrphidae), bees (Hymenoptera: Apoidea), and flowering plants were compared between harvested and unharvested hardwood stands in Algonquin Provincial Park, Ontario, Canada. Group-selection silviculture (where groups of trees are removed from a forested matrix, rather than single trees), increased the abundance of pollinators and flowering stems, but only after leaf-out. Wild red raspberry (Rubus strigosus) and bees benefitted most from the creation of canopy gaps. The combination of increased light, warm, bare soils, and abundant nectar-rich raspberry flowers probably created ideal habitat for soil-nesting bees, factors which are relatively absent from unharvested stands. By contrast, before leaf-out, spring ephemerals and high light-levels were universal and pollinators were even across treatments. More pollinators were caught in canopy gaps than in forested areas, and the proportion of fertilized ovules of spring beauty (Claytonia caroliniana) was higher in gaps than in the forest, suggesting that pollinators prefer foraging in gaps, even in spring. Group-selection silviculture in hardwood forests proved beneficial to native pollinating insects, at least in the short-term.

Genetic analysis of a host determination mechanism of bromoviruses in Arabidopsis thaliana

Brome mosaic virus (BMV) and Spring beauty latent virus (SBLV) are closely related, tripartite RNA plant viruses. In Arabidopsis thaliana, BMV shows limited multiplication whereas SBLV efficiently multiplies. Such distinct multiplication abilities have been observed commonly in all Arabidopsis accessions tested. We used this model system to analyze the molecular mechanism of viral resistance in plants at the species level. Unlike SBLV, BMV multiplication was limited even in protoplasts and a reassortment assay indicated that at least viral RNA1 and/or RNA2 determine such distinct infectivities. By screening Arabidopsis mutants with altered defense responses, we found that BMV multiplies efficiently in cpr5-2 mutant plants. This mutation specifically enhanced BMV multiplication in protoplasts, which depended on the functions of RNA1 and RNA2. In the experiment using DNA vectors to express BMV replication proteins encoded by RNA1 and RNA2, BMV RNA3 accumulation in cpr5-2 protoplasts was similar to that in wild-type Col-0 protoplasts, despite significant reduction of accumulation levels of replication proteins, suggesting that cpr5-2 mutation could enhance BMV multiplication independently of increased accumulation, therefore enhanced translation and stabilization, of the replication proteins.

Ancient Savannah Roots of the Carbohydrate Revolution in South-Central North America

Along the ecotone between North America’s southern Plains and southeast Woodlands, the oldest known earth ovens with rock heating elements date to 8,000–9,000 years ago. Through the millennia, the number and diversity of earth ovens increased markedly, but they typically functioned as plant-baking facilities, especially for geophytes, plants with underground storage organs. Among the important early Holocene geophytes in oak savannah regions were onions (Allium spp.) and camas (Camassia spp.). In the drier savannahs, including Texas’ Edwards Plateau, agave (Agave lechuguilla) and sotol (Dasylirion spp.), plants with above-ground storage organs, dominated oven cookery. Rock-filled earth ovens were common in comparatively wet regions with sandy soils, including Texas’ Post Oak Savannah, but floral preservation conditions are poor. Ethnobotanically and ecologically derived expectations, however, suggest false garlic (Northoscordum bivalve), onions, and spring beauty (Claytonia virginica) as potential plant food staples. Various plant foods were undoubtedly eaten throughout the pre-Columbian era but geophytes appear to have sparked and sustained an ancient carbohydrate revolution that led to significant early Holocene land-use intensification. Such a profound dietary shift may yet be shown to have played an important role in changes in Native American cranial morphology during the early Holocene.

Synthesis of infectious in vitro transcripts from Cassia yellow blotch bromovirus cDNA clones and a reassortment analysis with other bromoviruses in protoplasts

Cassia yellow blotch virus (CYBV), genus Bromovirus, was isolated from the Australian native legume, Cassia pleurocarpa, in western Queensland, and its host range was found to be distinct from other bromoviruses. In this study, CYBV was shown to infect systemically and efficiently a model plant species, Arabidopsis thaliana, as we recently reported for another bromovirus, Spring beauty latent virus (SBLV). We constructed full-length cDNA clones of CYBV genomic RNAs from which infectious in vitro transcripts can be transcribed, and determined their complete nucleotide sequences. CYBV RNA3 contains the box B motif in the intercistronic region, but lacks the subgenomic promoter-like sequence in the 5' noncoding region, as does Brome mosaic virus (BMV). To understand relationships among bromoviruses, we generated reassortants between CYBV and three other bromoviruses, BMV, SBLV and Cowpea chlorotic mottle virus. We found that all reassortants between BMV and CYBV accumulated viral RNAs to detectable levels in protoplasts of Nicotiana benthamiana, even when RNAs 1 and 2, which encode the replication proteins 1a and 2a, respectively, were heterologous. Sequence comparison and reassortment experiments of CYBV and other bromoviruses demonstrated that CYBV is closely related to BMV.

Identification and characterization of the SSB1 locus involved in symptom development by Spring beauty latent virus infection in Arabidopsis thaliana.

The natural variation of Arabidopsis thaliana in response to a bromovirus, Spring beauty latent virus (SBLV), was examined. Of 63 Arabidopsis accessions tested, all were susceptible when inoculated with SBLV, although there was a large degree of variation in symptom development. Most accessions, including Columbia (Col-0), were symptomless or developed only mild symptoms, but four accessions, including S96, showed severe symptoms of SBLV infection. Genetic analysis suggested that the difference in the responses of Col-0 and S96 to SBLV was controlled by a single semidominant locus. We have designated this locus SSB1 (symptom development by SBLV infection). By using genetic markers, SSB1 was mapped to chromosome IV. The patterns of distribution and accumulation of SBLV in sensitive accessions were similar to those in the insensitive accessions. In addition, symptom development in S96 by SBLV infection was critically interrupted by the presence of the NahG gene, which encodes salicylic acid (SA) hydroxylase. These data suggest that symptom development in A. thaliana controlled by SSB1 is independent of the efficiency of SBLV multiplication and is dependent on SA signaling.

EXPLOITATION OF POCKET GOPHERS AND THEIR FOOD CACHES BY GRIZZLY BEARS

I investigated the exploitation of pocket gophers (Thomomys talpoides) by grizzly bears (Ursus arctos horribilis) in the Yellowstone region of the United States with the use of data collected during a study of radiomarked bears in 1977-1992. My analysis focused on the importance of pocket gophers as a source of energy and nutrients, effects of weather and site features, and importance of pocket gophers to grizzly bears in the western contiguous United States prior to historical extirpations. Pocket gophers and their food caches were infrequent in grizzly bear feces, although foraging for pocket gophers accounted for about 20-25% of all grizzly bear feeding activity during April and May. Compared with roots individually excavated by bears, pocket gopher food caches were less digestible but more easily dug out. Exploitation of gopher food caches by grizzly bears was highly sensitive to site and weather conditions and peaked during and shortly after snowmelt. This peak coincided with maximum success by bears in finding pocket gopher food caches. Exploitation was most frequent and extensive on gently sloping nonforested sites with abundant spring beauty (Claytonia lanceolata) and yampah (Perdieridia gairdneri). Pocket gophers are rare in forests, and spring beauty and yampah roots are known to be important foods of both grizzly bears and burrowing rodents. Although grizzly bears commonly exploit pocket gophers only in the Yellowstone region, this behavior was probably widespread in mountainous areas of the western contiguous United States prior to extirpations of grizzly bears within the last 150 years.

Use of Spring beauty latent virus to identify compatible interactions between bromovirus components required for virus infection.

Spring beauty latent virus (SBLV) is a member of the genus Bromovirus, and is closely related to Brome mosaic virus (BMV) and Cowpea chlorotic mottle virus (CCMV). Compatible interactions between viral components are required for successful infection of plants by BMV and CCMV. To further our understanding of interactions between bromovirus components, we used SBLV to produce reassortants among the three bromoviruses. We found that SBLV RNA 2 functioned with heterologous bromovirus RNA 1 in infections of whole plants and protoplasts of Nicotiana benthamiana, although SBLV RNA 1 did not function with heterologous bromovirus RNA 2. A DNA-based transient assay for 1a and 2a proteins, which are encoded by RNAs 1 and 2, respectively further suggested that SBLV 2a protein may function in combination with heterologous bromovirus 1a protein. Moreover, analysis of the ability of reassortants to spread locally revealed that an RNA 2-mediated interaction between viral components may be required for efficient cell-to-cell movement of bromoviruses.

Further Examination of the RNA and Coat Protein of Spring Beauty Latent Virus

Spring beauty latent virus (SBLV) has been proposed as a new member of the bromovirus group. Four dsRNAs (M r 2.2, 2.0, 1.4, and 0.5× 10 6 ) with a profile similar to those of the bromoviruses were obtained when plant tissue infected with SBLV was analyzed. A fifth ds RNA (M r 1.2× 10 6 ) with nucleic acid similarity to the genomic RNA also was obtained. Molecular hybridization with cDNA to SBLV RNA did not show nucleic acid similarity between SBLV and four bromoviruses. Infectivity tests with SBLV ssRNAs indicated that it has a tripartite genome because RNAs 1, 2, and 3 were required for infectivity. When RNAs 1, 2, 36, and 4 were translated, 95, 48, and 23 kDa proteins were obtained (...)

Quantitative analyses of C-banded karyotypes, and systematics in the cultivated species of theScilla siberica group (Liliaceae)

Quantified C-band karyograms are presented for the related speciesScilla siberica, S. mordakiae, S. ingridae, S. amoena, andS. mischtschenkoana. Chromosome structure, banding style, and heterochromatin characters suggest a systematic grouping of two more closely related species pairs:S. siberica andS. mordakiae, S. ingridae andS. amoena; they are part of a larger aggregate, well separated fromS. mischtschenkoana. Four different heterochromatin fractions can be recognized inS. siberica and its relatives, but only two inS. mischtschenkoana. C-bands do not “replace”, but they are added to euchromatin. The particular origin and history of the cultivatedS. amoena and the triploidS. siberica cv. “Spring Beauty” appear to be responsible for their karyotype constancy, but chromosome conservatism obviously is genuine inS. mischtschenkoana. The banding data support the systematic grouping proposed on a morphological basis, and provide additional evolutionary evidence.

Three Chromosome Numbers for Claytonia Virginica (Portulacaceae)

From a restricted range in eastern Texas three chromosome numbers for Claytonia virginica L. are reported. Three individuals have been found with somatic numbers of 14, and one each with 15 and 28 chromosomes. No significant differences in gross morphology or pollen grain size were noted between these cytologically diverse individuals. The somatic chromosomes of five plants of the common Claytonia virginica L. (spring beauty) in eastern Texas have been examined. Flower buds were pre-treated for two and a half hours in PDB (para- dichlorobenzene), fixed in Carnoy's fluid (3 parts 95 per cent ethyl alcohol to 1 part glacial acetic acid), and squashed in 2 per cent acetic- orcein to obtain satisfactory mitotic plates. Localities and collectors of the plants are listed in Table 1 and are numbered from A to E. Three plants have somatic chromosome num- bers of 14, one individual has 15, and a third number of 28 is recorded for one plant (Figure 1). Note that the individual with 2n= 15 chromosomes was collected in the same population as one with 14 chromosomes. All individuals are indistinguishable by any gross morphological character. Pollen from each of the plants having different chromosome num- bers was stained in 1 per cent acetic-orcein and immediately measured across the widest diameter. Fifty pollen grains measured from each plant gave the following statistically summarized results: Plant A (2n= 14)---=63.6 (s= ?6.7, s-=f0.9) u, range 43.6 - 74.4 u; Plant D (2n=15)---=55.6 (s=_16.7, sx= 2.4) u, range 14.4-79.0 u; Plant E (2n = 28)--= 61.9 (s-= -8.6, si= 1.2) u, range 43.9- 79.9 u. From these data, no statistical difference can be noted in pollen size; in fact pollen from the plant with the highest chromosome number is smaller on the average than the plant with the smallest chromosome number. The aneuploid plant (2n= 15) has the smallest average pollen size due primarily to the micro-pollen occasionally measured in the random sample (as small as 14.4 u).