Spotted Halibut Verasper Variegatus Research Articles

A low body-color regulating ability of spotted halibut Verasper variegatus: Evaluation of the roles of melanin-concentrating hormone and proopiomelanocortin systems

A low body-color regulating ability of spotted halibut Verasper variegatus: Evaluation of the roles of melanin-concentrating hormone and proopiomelanocortin systems

Tumor necrosis factor-alpha (TNF-α) in spotted halibut Verasper variegatus at the embryonic and metamorphic stages

As an aquatic fish, the spotted halibut Verasper variegatus is highly susceptible to bacterial and virus infections. Tumor necrosis factor-alpha (TNF-α) as a cytokine could control the inflammatory responses. The functions of TNF-α in many species have been widely studied, particularly in mammals. However, little is known about the TNF-α functions in V. variegatus. We first cloned and sequenced the TNF-α gene in V. variegatus (VvTNF-α). The two conserved cysteine residues, transmembrane sequence, Thr-Leu motif, and TNF family signature, as well as the TA-rich motifs of its proteins related to inflammatory responses had high similarity to those of the other teleost and mammalian TNF-α. The phylogenetic analysis showed that VvTNF-α was consistent with TNF-α genes of other vertebrates. The VvTNF-α transcripts were extensively distributed in the peripheral blood leukocytes (PBLs), spleen, and gill, indicating that the VvTNF-α had a role in immune function. Furthermore, treatment with pathogen-associated molecular patterns (PAMPs) could induce a rapid and significant increase of VvTNF-α in the PBLs, which reveals that VvTNF-α does participate in the host immune responses against bacterial and viral pathogens. We found that VvTNF-α had an interesting expression pattern during metamorphosis, showing that the flatfish TNF-α may have some novel functions during specific developmental stages. In addition, the 3D structure prediction of VvTNF-α provided an indication of how it is likely to interact with other proteins. Therefore, VvTNF-α has multiple functions, and provides valuable information to explore novel functions of TNF-α.

Horizontal movement and emigration of juvenile spotted halibut Verasper variegatus released in a shallow brackish lagoon: Matsukawa-ura, northeastern Japan, revealed by acoustic telemetry

To elucidate the post-release movement and emigration of juvenile spotted halibut Verasper variegatus, ten hatchery-reared age-0 individuals (10 months old, 17.0–19.7 cm total length) surgically implanted with acoustic transmitters were released near the center of Matsukawa-ura, a shallow brackish lagoon, on 25 November 2009 (water temperature 13.8 °C). They were monitored by ten acoustic receivers for 5 months. Of ten individuals, eight left the release site during December–March. Three of these emigrated to the outer ocean on 17 and 29 January and 30 March 2010, respectively. Juveniles probably started wintering migration in December and January when water temperatures decreased considerably in the lagoon (mean 9.5 and 6.0 °C, respectively). They stopped their migration in the coldest month, February (mean 5.7 °C), and restarted it in March when water temperatures frequently exceeded a plausible threshold for movement (≥6 °C). Statistical analyses revealed that the fish started migration significantly more frequently at nighttime. The migration track of an individual recorded from 11 to 30 March showed gradual nocturnal movements and a slow migration speed (estimated maximum speed 2.2 m/min). Our results revealed that water temperature primarily governed the seasonal timing of nocturnal migratory movements of juvenile spotted halibut.

Path analysis of effects of morphometric traits on body weight in spotted halibut Verasper variegatus at different growth stages

PDF HTML阅读 XML下载 导出引用 引用提醒 不同生长期圆斑星鲽形态性状对体重影响的通径分析 DOI: 作者: 作者单位: 1. 中国水产科学研究院 黄海水产研究所, 山东 青岛 266071;2. 上海海洋大学 水产与生命学院, 上海 201306;3. 天津渤海水产研究所, 天津 300457 作者简介: 边力(1988-),男,助理研究员,博士,研究方向为鱼类繁育与遗传育种.E-mail:bianli@ysfri.ac.cn 通讯作者: 中图分类号: S96 基金项目: 天津市农业科技成果转化与推广项目（201604100）；中国水产科学研究院黄海水产研究所基本科研业务费（20603022016005）. Path analysis of effects of morphometric traits on body weight in spotted halibut Verasper variegatus at different growth stages Author: Affiliation: 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;3. Tianjin Bohai Sea Fisheries Research Institute, Tianjin 300457, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:圆斑星鲽（）作为中国鲽形目（Pleuronectiformes）养殖鱼类的重要代表种类，具有生长速度快，可食用部分多，肉质细嫩等特点。为了探究圆斑星鲽形态性状与体重的关系，本研究分别测定11月龄、14月龄和17月龄3个不同生长期圆斑星鲽的体重及全长（FL）、体长（BL）、体高（BH）、头长（HL）、头高（HH）、尾柄长（CL）、尾柄高（CH）、吻长（SL）共计8个形态性状，分别进行相关分析和通径分析，剖析形态性状对于体重的直接作用和间接作用；利用回归分析的方法，建立各个时期以形态指标为自变量，体重为因变量的最优线性回归方程。结果显示，1）不同生长阶段，影响体重的重点形态指标存在差异，影响11月龄圆斑星鲽体重的主要形态性状为体高和体长，14月龄阶段为全长、体高和头长，17月龄阶段为全长和体高；2）11月龄、14月龄和17月龄形态性状对体重（BL，XHL，；3）除本研究所包括的形态指标外，体厚可能为另外一个影响圆斑星鲽体重的重要指标。本研究阐明了体重与形态指标间的相关关系，为圆斑星鲽的良种选育提供了生物学依据。 Abstract:is an important representative species of the Pleuronectiformes. It has a fast growth rate, most parts of its body are edible, and the meat is tender and delicious; all these features make a good aquaculture candidate. In order to explore the relationship between morphometric traits and body weight in , full length (FL), body length (BL), body height (BH), head length (HL), head height (HH), caudal peduncle length (CL), caudal peduncle height (CH), snout length (SL), and body weight (BW) of at 11, 14, and 17 months of age were measured. Correlation and path analysis were conducted, and the effects of each morphometric trait on body weight were separated into direct and indirect effects. Regression analyses were conducted with morphological traits as independent variables and body weight as the dependent variable at each growth stage. The results showed that (1) different morphometric traits had significant path coefficients at each growth stage; at the 11 month stage, the morphometric traits that significantly affected body weight were BH and BL, at the 14 month stage, the significant morphometric traits were FL, BH, and HL, at the 17 month stage, the significant traits were FL and BH. (2) The regression equations at 11, 14, and 17 months of age were =-362.484+13.145, and , respectively. (3) Besides the morphometric traits included in this study, body thickness is another potentially important trait that can affect body weight. This study demonstrated the relationship between morphometric traits and body weight. The results provide valuable information and theoretical guidance for breeding programs. 参考文献 相似文献 引证文献

Characterization of the global transcriptome and microsatellite marker information for spotted halibut Verasper variegatus

The spotted halibut Verasper variegatus is an economically important flatfish species distributed in Japan, Korea and China. However, the genomic resources regarding this species were scarcity, which hindered our understanding of the genetics and biological mechanisms in spotted halibut. In this study, we examined the global transcriptome from six major tissues of spotted halibut. Approximately 40 million of high quality reads were generated using Illumina paired-end sequencing technology. More than 9 Gbp data were generated, and de novo assembled into 59,235 unigenes, with an N50 of 938 bp. Based on sequence similarity search with known protein database, 34,084 (57.5%) showed significant similarity to known proteins in Nr database, and 28,875 (48.7%) had BLAST hits in Swiss-Prot database. 19,562 and 23,037 unigenes were assigned into gene ontology categories and clusters of orthologous group, respectively. 9138 unigenes were mapped to 211 KEGG pathways. For functional marker development, 13,322 candidate simple sequence repeats were identified in the transcriptome and 7235 primer pairs were successfully designed. Among 72 primer pairs selected for validation, 67 (93.1%) were successful in PCR amplification and 14 (19.4%) exhibited obvious repeat length polymorphisms in a culture spotted halibut population. The transcriptomic data and microsatellite markers will provide valuable resources for future functional gene analyses, genetic map construction, and quantitative trait loci mapping in V. variegatus.

Collection record of spotted halibut Verasper variegatus juveniles from the central Seto Inland Sea, Japan

Collection record of spotted halibut Verasper variegatus juveniles from the central Seto Inland Sea, Japan

Effects of Methyltestosterone on Masculinization in Larva and Juvenile of the Spotted Halibut Verasper variegatus

Effects of Methyltestosterone on Masculinization in Larva and Juvenile of the Spotted Halibut Verasper variegatus

Feeding, Growth, and Dispersal of Hatchery-reared Spotted Halibut Verasper variegatus Released in a Shallow Brackish Lagoon: Matsukawa-ura, Northeastern Fukushima, Japan

Feeding, Growth, and Dispersal of Hatchery-reared Spotted Halibut Verasper variegatus Released in a Shallow Brackish Lagoon: Matsukawa-ura, Northeastern Fukushima, Japan

Effects of Water Temperature on Survival and Sex Differentiation in Hatchery-reared Spotted Halibut Verasper variegatus

Effects of Water Temperature on Survival and Sex Differentiation in Hatchery-reared Spotted Halibut Verasper variegatus

III-3. ホシガレイの管理単位と遺伝的リスク

III-3. ホシガレイの管理単位と遺伝的リスク

III-2. 東北海域におけるホシガレイの栽培漁業

III-2. 東北海域におけるホシガレイの栽培漁業

Erratum to: Egg collection from hatchery-reared broodstock of spotted halibut Verasper variegatus treated with LHRH analog

The objective of this study is to establish a method for acquiring large quantities of high-quality eggs for artificial fertilization from hatchery-reared broodstock of spotted halibut Verasper variegatus. We estimated the optimum conditions for implantation of luteinizing hormone-releasing hormone analog (LHRHa) cholesterol pellet (LHRHa-CP) to induce ovulation from two experiments which focused on the dose and the timing combined with monitoring of oocyte development by ovarian cannulation. From the results of cannulation, vitellogenesis of hatchery-reared broodstock was suggested to occur normally, although final oocyte maturation was not initiated in the first clutch of oocytes. In late December, oocytes in the most advanced clutch still underwent vitellogenesis, having diameter of about 0.82 mm. LHRHa-CP implantation during this period had no remarkable effects, except for administration at high dose (100 μg/kg). In contrast, in mid January, when oocyte diameter reached about 0.95 mm, ovulation occurred in most individuals, even at low dose (20 μg/kg). In mid February atretic oocytes became remarkable and LHRHa-CP implantation showed much lower performance in terms of egg quality. The diameter of growing oocytes converged to about 0.95 mm, which was that of fully grown postvitellogenic oocytes. Thus, oocyte diameter is suggested to be an effective indicator to estimate the timing of LHRHa-CP implantation.

Effectiveness of stock enhancement of a rare species, spotted halibut Verasper variegatus, in Fukushima, Japan

The effectiveness of stock enhancement trials of a rare flatfish Verasper variegatus was evaluated through assessment of fishery statistics, geographical distributions of catches, and intensive market surveys in Fukushima Prefecture, Japan. In all, 426,704 cultured individuals within 23 age-0 cohorts and 4 older cohorts (7.5–10.8cm and 20.0–51.0cm total length, respectively) were released at six coastal sites off Fukushima between 1993 and 2007. Commercial landings before the release trials decreased from 5.3metric tons (MT) in 1987 to 1.0MT in 1993. After the release trials, commercial landings increased rapidly to 2.7±0.6MT during 1996–2010 (maximum of 3.8MT in 2008). Fish of 25–70cm total length (mainly 1–3years old) were caught equally by gillnet and trawl fisheries at different depth ranges (mainly <50m and 100–150m), corresponding respectively to those of feeding and spawning grounds. The numerical contribution of released fish increased rapidly, exceeding 70% during the mid-2000s; up to 91% in 2004. Multiple regression analysis revealed two factors (number released and release date from first January) that significantly and negatively affected the recapture rates of age-0 cohorts (0–24.8%, mean 6.0%). Our results demonstrate that local stocks of a rare species can be augmented through the release of cultured juveniles. In addition, the strong direct impacts of hatchery releases (rapid increase of landings and contribution rate) suggest that stocking effectiveness of a rare species were less influenced by the fluctuating natural populations than common species are. Results also suggest that the long-term and comprehensive assessments of release trials from release, to fishery catches, and to market landings are necessary to detect potential factors affecting recapture rates and to clarify the ecological characteristics of a rare species. The release strategy can be refined based on the feedback process of these results.

Egg collection from hatchery-reared broodstock of spotted halibut Verasper variegatus treated with LHRH analog

Egg collection from hatchery-reared broodstock of spotted halibut Verasper variegatus treated with LHRH analog

Induction of ambicoloration by exogenous cortisol during metamorphosis of spotted halibut Verasper variegatus

Cortisol, the main glucocorticoid in fish, increases during flatfish metamorphosis and peaks before the surge of thyroxine. A large body of evidence indicates the essential role of thyroxine in flatfish metamorphosis, whereas information on cortisol is limited. We administered cortisol to spotted halibut Verasper variegatus larvae in order to examine the effect on pigmentation during metamorphosis. Administration of 10 μg cortisol per mL of water from before the onset of metamorphosis (stage E) to metamorphic climax (stage G) induced the development of adult type pigment cells on the blind side of the metamorphosed juveniles and increased the occurrence of ambicolored juveniles. When 10 μg/mL cortisol was administered during stage D, stages E-F, stage G or stage H, only the administration during stages E-F induced the development of adult type pigment cells on the blind side. In addition, the expression of the gene dopachrome tautomerase (dct), a marker of melanoblasts, was enhanced at Stage E by cortisol administration. These results clearly indicated, for the first time, the enhancement of pigmentation by exogenous high-dose cortisol. Since endogenous cortisol is secreted in response to various kinds of stress in rearing conditions, these results indicate a possible influence of stress conditions in the occurrence of ambicoloration in flatfish.

Habitat utilization, feeding, and growth of wild spotted halibut Verasper variegatus in a shallow brackish lagoon: Matsukawa-ura, northeastern Japan

Habitat utilization, feeding, and growth of a rare pleuronectid flatfish, spotted halibut Verasper variegatus, were examined in a brackish lagoon in northeastern Japan: Matsukawa-ura. The distribution and date–length data of spotted halibut collected mainly from beam-trawl samplings during 1983–2008 indicated that age-0 juveniles [n = 25, 6.0–18.0 cm total length (TL)] and older spotted halibut (n = 71, 13.8–43.0 cm TL) inhabited almost the entire northern part of Matsukawa-ura. Comparative distribution surveys of spotted halibut, stone flounder, and marbled flounder during 1985–1989 revealed similar distribution patterns of these flatfishes; the highest densities were found around the central part of Matsukawa-ura, although significantly lower abundance was detected for spotted halibut. Linear growth equations of age-0 juveniles demonstrated that spotted halibut were able to achieve high growth after June, probably because abundant prey (e.g., mysids and gammarids) and suitable physical conditions (warmer water temperature and lower salinity) were present. The main prey items shifted from various crustaceans including mysids, caridean shrimps, and anomurans to brachyura Hemigrapsus spp. (≥20 cm TL). Our study shows that spotted halibut use the shallow brackish lagoon as an important nursery for juveniles, and also as a feeding ground for young and adults.

Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis

The major histocompatibility complex (MHC) is a large genomic region characterized by extremely high polymorphism and its association with resistance/susceptibility to disease in vertebrates. In this study, the full lengths of MHC IIA and IIB cDNA were obtained from spotted halibut (Verasper variegates) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) approaches. The genomic structure, molecular polymorphism, and expression patterns were examined to study MHC II gene functions in fish. As in other teleosts, the genomic structure of the spotted halibut MHC IIA contained 4 exons and 3 introns. The deduced amino acid sequence of the class IIA molecule shared 28-79% similarity with those of teleosts and mammals. Nine class IIA alleles were identified from five individuals. Three alleles originating from a single individual suggested the existence of at least two class IIA loci in the genome. Six exons and 5 introns were identified from spotted halibut MHC IIB, and the deduced amino acid sequence shared 33-79% similarity with those of teleosts and mammals. Twelve alleles were identified, among which five were observed in a single individual, which suggested at least three class IIB loci. Quantitative real-time PCR analysis revealed the presence of class IIA and IIB transcripts in nine normal tissues with high expression level in kidney and gill. Furthermore, MHC IIA and IIB are probably two candidates of immune molecules involved in the acute-phase response in spotted halibut, because their transcriptional levels were significantly up-regulated in blood and liver after bacterial challenge.

Genetic structure in species with shallow evolutionary lineages: a case study of the rare flatfish Verasper variegatus

We examined the genetic population divergence of the spotted halibut Verasper variegatus. A previous report suggested two conservation units for this species along the Japanese Pacific coast. Extending the coverage of the genomes (29 microsatellites and three mitochondrial DNA segments) revealed hitherto-undetected genetic population boundaries. We screened population samples from the major habitats along the Japanese coast and the Yellow Sea coast (East Asian Continent). Significant genetic differentiation was found in every comparison between the habitats. In most cases, the nuclear and mitochondrial population divergences were incongruent, most likely caused by differences between the two genomes in the effects of genetic drift after recent population isolation and bottleneck events. We discuss the ecological and evolutionary mechanisms of the genetic structure as well as the units of conservation. The present study illustrates the merits of wider coverage of genomes in genetic population analysis especially for species with a shallow population history.

Successful stocking of a depleted species, spotted halibut Verasper variegatus, in Miyako Bay, Japan: evaluation from post-release surveys and landings

Post-release adaptation and stocking effectiveness of a highly depleted pleuronectid flatfish, spotted halibut Verasper variegatus, were assessed in Miyako Bay, Japan. In early July 2004 and 2005, 22 000 and 5000 hatchery-reared juveniles (mean 81.2 and 89.8 mm total length, respec- tively) were released in shallow waters in the inner bay area. Results from surveys with a 2 m beam- trawl conducted over a 2 mo post-release period, laboratory predation experiments using potential predators (age-1 Japanese flounder), and a market census continued until December 2008, suggested that released juveniles, which had a size refuge from predators, could quickly adapt to brackish water habitats where there were suitable physical conditions (temperature, salinity and sediments) and abundant prey organisms. Juveniles selectively preyed on epibenthic gammarids and cumaceans, and had full stomachs 1 wk after release. Some released fish grew to >40 cm by age-1+, feeding exclusively on larger crustaceans (e.g. isopods, carideans and brachyurans). Market census revealed that released fish showed >99% contribution to catches, and recapture rates for fish released in 2004 (7.6%) and 2005 (15.7%) were higher than for releases in 2000 to 2003 (0.7 to 5.0%), possibly due to the development and application of improved release strategies (i.e. release habitat, size and season was appropriate in 2004 and 2005). Our study demonstrates that (1) local depleted stocks can be effectively restored by hatchery releases and that (2) development of release strategies based on the analysis of ecological interactions between released juveniles and the target ecosystem can largely contribute to the successful restocking of depleted species.

Effects of Dietary Protein and Energy Levels on Growth, Feed Conversion and Body Composition of Spotted Halibut Verasper variegatus

Effects of Dietary Protein and Energy Levels on Growth, Feed Conversion and Body Composition of Spotted Halibut Verasper variegatus