Spot Urine Protein Creatinine Ratio Research Articles

A Comparison of the Spot Urine Protein-Creatinine Ratio with 24-h Urinary Protein for Quantification of Proteinuria: A Hospital-based Cross-sectional Study.

Quantifying the amount of proteinuria is mandatory in various disease conditions. The aim of this study was to study whether the spot urine protein-creatinine ratio (P-CR) correlates well with 24-h urinary total protein (UTP). The research hypothesis was that spot urine P-CR would correlate well with 24-h UTP. This was a cross-sectional, single-center study conducted in a tertiary care hospital. The spot urinary P-CR and 24-h urinary protein were determined from 70 patients with persistent glomerular proteinuria. This study included Nepalese patients aged 2-83 years, with a mean age of 36.56 years (standard deviation: 20.78). The number of males was slightly higher than females, and the male-female ratio was 1.26:1. Hypertension was present in 44.3% of patients, diabetes was present in 20% of patients, 74.3% of patients were suffering from acute glomerulonephritis with various causes, and 12.9% of patients had chronic kidney disease. A linear relationship existed between the spot urine P-CR and the 24-h UTP, with a correlation coefficient of 0.877 (P <0.01). The correlation was suboptimal at higher levels of protein excretion (>3.5 g/day). Random spot urine P-CR correlated well with the 24-h UTP, particularly at lower levels of protein excretion.

Assessing the Accuracy of Diagnostic Tests to Quantify Proteinuria in Nephrotic Children: A Cross-Sectional Study

Objective: To assess the diagnostic accuracy of 24-hour proteinuria estimation and urine dipstick-taking spot urine protein creatinine ratio as the gold standard in children with steroid-sensitive nephrotic syndrome. Study Design: Cross-sectional analytical study. Place and Duration of Study: Department of Paediatric Nephrology, Sindh Institute of Urology and Transplantation, Karachi Pakistan, from Oct 2020 to Mar 2021. Methodology: Proteinuric children were enrolled to quantify proteinuria by 24-hour urine protein estimation, spot urine protein creatinine ratio and urine dipstick. Sensitivity analysis was performed, and receiver operating curves were plotted to assess the diagnostic accuracies of 24-hour proteinuria and urine dipstick against spot urinary protein creatinine ratio. Scatter plots compared the correlation of serum albumin and cholesterol with 24-hour urine protein estimation and spot urine protein creatinine ratio. Results: Forty-two children with a median age of 8 years (IQR 6–10) were included. Nephrotic range proteinuria was detected in 39(93%) children with spot ratio, 16(38%) cases using 24-hour proteinuria estimation and 50% with a urine dipstick.Twenty-four-hour protein estimation showed a sensitivity of 63.4%, and urine dipstick had a sensitivity of 53.8% in detecting nephrotic range proteinuria compared to spot ratio with a negative predictive value of 6.3% and 14.3%, respectively.Hypoalbuminemia and cholesterol correlated better with spot ratio than 24-hour proteinuria with r- values 0.0143 and 0.0713, respectively. Conclusion: Twenty-four-hour urine protein estimation and dipstick correlate with spot urine protein creatinine ratio in detecting nephrotic proteinuria with no statistical difference in diagnostic accuracy.

The role of a low protein diet supplemented with ketoanalogues on kidney progression in pre-dialysis chronic kidney disease patients

In slowing kidney progression, numerous pre-dialysis chronic kidney disease (CKD) patients could not adhere to the well-established dietary pattern, including a very low protein diet, 0.3–0.4 g/kg/day, plus a full dose ketoanalogues (KAs) of amino acids. We evaluated the role of a low protein diet (LPD), 0.6–0.8 g/kg/day, combined with KAs (LPD–KAs) on CKD progression. We extracted data in the retrospective cohort using electronic medical records (n = 38,005). Participants with LPD–KAs for longer than six months were identified. An unmatched control group, LPD alone, was retrieved from the same database. Cox proportional hazard models were performed to examine the associations between LPD–KAs and outcomes. The primary outcome was either a rapid estimated glomerular filtration rate (eGFR) decline > 5 mL/min/1.73m2/year or commencing dialysis. Other secondary outcomes include changes in proteinuria, serum albumin, and other metabolic profiles were also assessed. A total of 1042 patients were finally recruited (LPD–KAs = 543). Although patients with LPD–KAs had significantly lower eGFR and a prevalence of diabetes, age, and dietary protein intake were comparable between LPD–KAs (0.7 ± 0.2 g/kg/day) and LPD alone groups (0.7 ± 0.3 g/kg/day, p = 0.49). During a median follow-up of 32.9 months, patients treated with LPD–KAs had a significantly lower risk of kidney function decline (HR 0.13; 95% CI 0.09–0.19, p < 0.001) and dialysis initiation (HR 0.24; 95% CI 0.12–0.49, p < 0.001) than LPD alone after adjusting for confounders. The annual rate of eGFR decline in patients receiving LPD–KAs was 4.5 [3.4–5.5] mL/min/1.73m2 compared with 7.7 [6.0–9.4] mL/min/1.73m2 in LPD alone (p = 0.001). According to KAs dose–response analysis, the daily dose of ≤ 5 tablets was conversely associated with a higher risk of the primary endpoint, whereas the association disappeared among patients receiving a dose of > 6 tablets. The spot urine protein creatinine ratio and serum phosphate levels were not significantly different between groups. LPD–KAs could retard kidney progression compared with LPD alone. This favorable effect was significant among CKD patients receiving a daily KAs dose of more than six tablets. Future randomized controlled trials should be performed to verify these findings.

A Syudy on Comparative Evaluation of Four Different Methods to Quantify Proteinuria in Pre-Eclampsia in a Tertiary Care Hospital

Background: Preeclampsia is a progressive, multisystem disorder due to spasm of small blood vessels of all organ systems characterized by new-onset hypertension and end- organ dysfunction in the last half of pregnancy. Objectives: Estimation of proteinuria by sulphosalicylic acid test in women with preeclampsia. Material &amp; Methods: Prospective hospital based clinical study. Study conducted in the Department of Obstetrics and Gynaecology, Murshidabad Medical college, Berhampore, Murshidabad, West Bengal. Sep. 2021 - Aug. 2022. Pregnant women after 20weeks of gestation diagnosed as preeclampsia and admitted in antenatal ward in obstetrics and gynaecology department, Murshidabad Medical college, Berhampore, Murshidabad, West Bengal. Study consisted a total of 100 subjects. Simple Random sampling method. The pregnant women diagnosed as preeclampsia meeting the inclusion and exclusion criteria included in the study. The study comprised of pregnant women who are more than 20 weeks of gestation diagnosed as preeclampsia fulfilling the inclusion and exclusion criteria. Written and informed consent was taken from all pregnant women participating in the study. The pregnant women in the study were subjected to a detailed history and general abdominal and pelvic examination. A first voided morning urine sample was obtained for sulphosalicylic acid test, dipstick test, spot urine PCR. Subsequent urine samples were collected for 24 h, including a next-day first-morning voided sample for the 24-h urine protein estimation. Results: On assessment of the ROC curve to assess the predictability of three methods for spot urine compared against the 24hr urine protein estimation, the AUC for the Sulphosalicylic acid was found to be significantly better (AUC=0.970, p&lt;0.05) compared to the urinary PCR (AUC=0.964, p&lt;0.05) and for urinary dipstick it was AUC=0.784, p&lt;0.05. Conclusion: This study concluded that spot urine sulphosalicylic acid and urine protein creatinine ratio are reliable investigations compared to the dipstick method to assess the proteinuria in hypertensive pregnant women or for diagnosis of pre-eclampsia. So, spot urine Sulphosalicylic acid and urine protein-creatinine ratio can be used for detection of proteinuria in pregnant women with suspected pre-eclampsia with high accuracy, which is more rapid than time consuming 24-hour urine protein estimation

Comparison of Spot Urinary Protein Creatinine Ratio and 24 hour Urinary Protein Excretion in Children presenting with Nephrotic Syndrome in Tertiary Care Hospital of Eastern Nepal

Introduction: Nephrotic syndrome is an important chronic disorder in children and it’s one of the important diagnostic criteria is presence of heavy proteinuria (&gt; 40 mg/m2/hour). As 24-hour urinary protein estimation is cumbersome, inconvenient, time consuming and expensive, a more convenient and accurate method of urinary protein estimation is needed. 24-hour urinary protein estimation and urine protein/creatinine in a child with nephrotic syndrome correlates well but there are very few studies done in Nepal to prove this correlation. Hence, this study is undertaken with objective of evaluating usefulness of urine protein/creatinine (UP/UC) in random sample of urine as a rapid and reliable test for quantification of proteinuria and to know their correlation with 24hour urinary protein excretion. Objectives: Primary Objective: To evaluate accuracy of urine protein creatinine ratio (UP/UC) in early morning sample in comparison with 24 hours urinary protein excretion in children of nephrotic syndrome having normal Glomerular Filtration Rate. Secondary Objective: To evaluate usefulness of urine protein / creatinine ratio (UP/UC) in random sample of urine as rapid and reliable test for quantification of proteinuria. To evaluate biochemical and other laboratory abnormalities in children with nephrotic syndrome. To study varied clinical presentation of Pediatric nephrotic syndrome Methodology: This is a descriptive cross-sectional study conducted in pediatric unit, Nobel Medical College Teaching Hospital, Biratnagar for 12 months. In this study, 50 patients of both sexes, ranging from one to fifteen years of age were studied. The modes of presentation, laboratory investigation reports which included urine routine microscopy, 24-hour urine protein estimation, urine protein / creatinine in random sample of urine were documented and data was analyzed by linear regression. Results: Linear regression revealed that as timed 24-hour urine protein in gm/24 hour increased, Random urine/protein creatinine ratio mg/mg also increased linearly with correlation coefficient of r = 0.56 which was highly significant (p &lt; 0.001). Conclusion: This study concludes that UP/UC ratio in a spot urine reflects the amount of protein in 24-hour urine collection. UP/UC ratio &gt; 2 in patients with normal renal function represents nephrotic range proteinuria.

#4356 DYNAMIC EFFECT OF PROTEINURIA ON ADVERSE KIDNEY OUTCOMES WITH TARGETED MAXIMUM LIKELIHOOD ESTIMATION METHOD: RESULT FROM THE KNOW-CKD STUDY

Abstract Background and Aims Proteinuria is a risk factor for the progression of chronic kidney disease (CKD), but its causal inference may be challenging due to time varying exposure and confounding, the changing its degree, and loss to follow up. Method A total of 1,223 patients enrolled as part of The Korean Cohort Study for Outcome in Patients With Chronic Kidney Disease (KNOW-CKD) were analyzed at 3 observational points (baseline, 3-year; early, and 7-year; late) at which spot urine protein creatinine ratio (UPCR) was measured. We used longitudinal targeted minimum loss-based estimation (TMLE) and marginal structural models (MSM) to estimate the cumulative incidence of adverse kidney outcomes (eGFR halving and kidney failure requiring replacement therapy) of dynamic exposure to high proteinuria (UPCR≥1g/g) comparing counterfactuals (UPCR&amp;lt;1g/g) adjusted for time varying confounding (systolic blood pressure, estimated glomerular filtration rate, and renin-angiotensin-aldosterone blockers) and baseline covariates (age, sex, diabetes, cardiovascular disease, and smoking). Results Patients with sustained high proteinuria throughout 7-year follow-up had significant higher risk of renal events than those with counterfactuals (relative risk [RR], 3.120; 95% confidence interval, 2.150-4.528; P &amp;lt; 0.001). The RR were 0.168 (0.055-0.517; P = 0.002) and 0.613 (0.337-1.114; P = 0.108) for early and late proteinuria reduction comparing sustained high proteinuria, respectively. In MSM, hazard ratio (HR) of accumulative high proteinuria frequency were 1.612 (1.463-1.844; P &amp;lt;0.001) up to 3 years and 1.102 (0.877-1.387; P = 0.404) up to 7 years, respectively. Conclusion In CKD patients, sustained higher proteinuria is the main risk factor during the entire observation period. However, considering dynamic exposure, proteinuria reduction from the beginning has a protective effect on renal progression, but the effect weakens as the observation period becomes longer.

A Comparison of Urine Dipstick Test with Spot Urine Protein-Creatinine Ratio and 24 Hour Urine Protein Excretion in Women with Hypertensive Disorders of Pregnancy

A Comparison of Urine Dipstick Test with Spot Urine Protein-Creatinine Ratio and 24 Hour Urine Protein Excretion in Women with Hypertensive Disorders of Pregnancy

The Correlation of Urine Protein/osmolality and Protein/creatinine Ratio as Predictor of 24-hour Urinary Protein Excretion.

The drawback of 24-hour urine collection is that it is troublesome, takes a lot of time, and is inaccurate because of collection errors and improper timing. The research in the Indian adult population about the correlation of spot urine protein/osmolality ratio (UPOR) and spot urine protein/creatinine ratio (UPCR) as a predictor of 24-hour urinary protein excretion is lacking. To study the correlation of spot UPOR and spot UPCR as the prognosticator of 24-hour urinary protein excretion. The present cross-sectional observational study was undertaken on 50 patients &gt;18 years of age and of either sex who presented with proteinuria (dipstick test or urine routine) and were advised 24-hour urine protein examination. A 24-hour urine was collected for protein analysis starting from any time of day. Random urine samples were collected and processed for protein, creatinine, and osmolality. The spot UPOR and UPCR were calculated. The sensitivity and specificity were determined. Pearson's correlation was used to find the correlation. There was a statistically significant positive correlation of UPOR and UPCR (r = 0.418 and r = 0.512, respectively) with 24-hour urinary protein excretion. The sensitivity and specificity of UPOR to predict 24-hour urinary protein at cutoff point 1.32 was 82.3% and 81%, respectively. The sensitivity and specificity of UPCR to predict 24-hour urinary protein at cutoff point 1.09 was 87 and 86%, respectively. For the medical determination of proteins in urine, spot UPOR and UPCR are suitable, cheap, and dependable methods that can substitute the measurement of 24-hour urine protein.

Correlation between 24-hour Urinary Total Protein Excretion and Single Urinary Protein Creatinine ratio in the Diagnosis of Childhood Nephrotic Syndrome : Study in a Tertiary Level Hospital

Introduction:Protein excretion varies in the course of the day, for this reason 24-hours urinary total protein excretion has been considered as the classic reference method for protein determination in nephotic syndrome. As the collection of urine for 24 hours is tedious and errors may occur during the process, so the protein:creatinine ratio was developed as a diagnostic alterative for Nephrotic syndrome.The main aim of this study is to determine the correlation between 24-hour urinary total protein excretion and single urinary protein creatinine ratio in childhood Nephrotic syndrome. Methods: This cross-sectional study was conducted in the department of Paediatrics,Shaheed Suhrawardy Medical College &amp; Hospital from June 2018 to May 2019. After taking valid consent total 60 cases of Nephrotic syndrome were selected purposively according to inclusion and exclusion criteria. Here we tried to determine the correlation between 24-hour urinary total protein excretion and single urinary protein creatinine ratio in the diagnosis of childhood Nephrotic syndrome by measuring the spot urinary protein creatinine ratio and the 24 hours urinary total protein excretion of the patients. Results : Among total 60(N) children of Nephrotic syndrome 42 (70%) patients were male and 18 (30%) patients were female with M:F = 2.16:1.The mean age was 4.57 ± 2.01SD years. Most of the patients were presented with some associated diseases like urinary tract infection (30%), upper respiratory tract infection (28.3%), viral illness(8.3%), atopic dermatitis(3.3%) and nonspecific illnesses(30%). The main physical findings were only puffy face(100%), with ascites (86.6%) and swelling of genitalia (38.3%).The mean 24 hours urinary total protein excretion was 3114.45 ± 1627.89 mg/24 hours and spot urinary protein creatinine ratio was 5.57 ± 3.13 SD. There was a very significant correlation between 24 hour total urinary protein excretion and spot urinary protein creatinine ratio. The sensitivity of the study was 80.49 – 100% and specificity of 91.78 - 100% at £2000 mg /sqm body surface area/ day protein excretion. The p value was .002 with correlation coefficient was 0.39. Conclusion: The protein creatinine ratio of a random urine sample might be used as an alternative method for the diagnosis of Nephrotic syndrome in children in view of the obvious advantages in terms of cost, time and patient convenience. J Shaheed Suhrawardy Med Coll 2021; 13(2): 137-142

Study on correlation of urine specific gravity with other laboratory parameters of urine in children with Nephrotic syndrome

Nephrotic syndrome is a frequent childhood kidney disease in which proteinuria is a significant characteristic. Proteinuria can be a benign condition as well as indicates severe underlying renal or systemic diseases. The variation in the prevalence of proteinuria may occur based on the definition used or the evaluation time of the test. Proteinuria is present in up to 10% of routine urine testing in school-aged children, although this decreases to 0.1% with repeated testing. The study aimed to analyze the correlation of urinary specific gravity with other laboratory parameters of urine in children with nephrotic syndrome. Methods: This cross-sectional study was conducted in the Department of Pediatric Nephrology (NIKDU), Dhaka from October 2019 to June 2021, and a total of 153 patients with nephrotic syndrome were enrolled after taking written consent. Urine samples were collected for both 24 hours and spot for estimation of urinary protein, urinary creatinine, dipstick protein, and specific gravity by dipstick method. Spot urinary Protein Creatinine Ratio (PCR) and 24 hours urinary protein were also estimated. Results: Among 153 study subjects maximum (44.4%) were 2-5 years old followed by 58 (37.9%) who were 5-10 years and 27 (17.6%) were &gt;10 years old. Boys (61.4%) were more predominant than girls (38.6%). Mean 24 hours urinary volume was 1097.78±662.63 ml, 24 hours urinary creatinine was 32.17±23.70 mg/dl, 24 hours urinary total protein was 851.42±123.68 mg/dl, 24 hours urinary total protein (UTP) was 7.52±7.65 gm/m2/24 hours urinary specific gravity 1.020±0.02, spot urinary protein 989.49±1136.73 mg/dl, spot urinary creatinine was46.09±43.73 mg/dl, spot urinary PCR was 21.87±18.24 mg/mg and spot urinary specific gravity was 1.020±0.01. The Scatter diagram showed a significant positive correlation of 24 hours urinary specific gravity with 24 hours urinary protein (r=0.169, p=0.037). There was a significant positive correlation of 24 hours specific gravity with 24 hours urinary creatinine (r=0.380, p=&lt;0.001). There was a significant positive correlation of spot urinary protein with spot urinary specific gravity as well (r=0.206, p=0.011). Spot urinary specific gravity showed a significant positive correlation with spot urinary creatinine (r=0.355, p=&lt;0.001). There was a significant negative correlation of spot urinary specific gravity with spot urinary PCR (r= -305, p=&lt;0.001). Conclusion: It can be concluded that urinary specific gravity had a substantial correlation with other laboratory parameters of urine and can be used as an important diagnostic tool for diagnosing proteinuria in children with nephrotic syndrome

Effect of deferasirox on renal function in thalassemic children

Abstract Background: Iron-chelating therapy causes improvement in the life expectancy of thalassemic patients. Deferasirox (DFX) is an oral iron chelator with an established dose-dependent efficacy, though alteration of renal function has been observed in many studies. In the present study, we evaluated the effect of treatment with DFX on renal function. Materials and Methods: This quasi-experimental study was done in the Thalassemia Center of Bangladesh Shishu Hospital and Institute from June 2019 to July 2021, where 50 thalassemia major and E-β thalassemia children aged 2–18 years old were included. Blood urea nitrogen (BUN), serum creatinine, spot urinary protein creatinine ratio, spot urinary calcium creatinine ratio, and estimated glomerular filtration rate (eGFR) were measured. If renal functions were normal, DFX was started at a dose of 25 mg/kg/day. After 6 months of DFX treatment, the abovementioned investigations were repeated and analysis was done. The study variables were analyzed by paired t-test. P value &lt;0.05 was considered significant. Results: The mean age of the thalassemic children was 5.21 ± 1.72 years. Before treatment, mean serum creatinine was 39.78 ± 3.12 μmol/L, and after 6 months, serum creatinine was increased but not statistically significant. The mean value was 40.50 ± 3.39 μmol/L after 6 months (P value 0.071). There was no significant change of BUN. Initially, the mean BUN was 3.39 ± 0.76 mmol/L, and 6 months after treatment, it was 3.45 ± 0.70 mmol/L. Initially, the spot urinary protein creatinine ratio was 0.12 ± 0.04, and after 6 months, the mean value was 0.43 ± 0.24. it was significantly increased (P value &lt;0.001). Initially, the spot urinary calcium creatinine ratio was 0.068 ± 0.043, and after treatment, it was significantly raised. The mean value was 0.191 ± 0.168 (P value &lt;0.001). After 6 months of treatment with DFX, 35 patients developed mild proteinuria, and eight patients developed hypercalciuria. There was no significant change of eGFR before and after treatment. Conclusions: In this study, we got significant proteinuria and hypercalciuria but nonsignificant changes in serum creatinine, BUN, and eGFR.

Assessment of Correlation of Serum Lipid Profile and Urinary Protein Creatinine Ratio with Occurrence of Gestational Hypertension in Pregnant Mothers in a Tertiary Care Hospital, Kolkata

After 18 months study from 1st April, 2021 to 30th September, 2022 on the pregnant mothers in a tertiary care hospital, i.e., in R.G. Kar Medical College &amp; Hospital, Kolkata, we have observed that there is significant rise of Urinary Protein Creatinine ratio in spot urine sample of Gestational hypertensive women in comparison to normotensive pregnant women. We may opine that, measurement of Spot Urinary Protein Creatinine Ratio as well as Serum Triglyceride, Total Cholesterol, different components of Cholesterol and calculation of their ratios may be used as precautionary steps, to take the necessary actions to prevent the complications of Gestational hypertension, i.e., graver grades of Pregnancy Induced Hypertension (PIH), namely, Preeclampsia and Eclampsia.

Clinical value and cost analysis of the sFlt-1/PlGF ratio in addition to the spot urine protein/creatinine ratio in women with suspected pre-eclampsia: PREPARE cohort study

BackgroundThis study investigated the clinical value of adding the sFlt-1/PlGF ratio to the spot urine protein/creatinine ratio (PCr) in women with suspected pre-eclampsia.MethodsThis was a prospective cohort study performed in a tertiary referral centre. Based on the combination of PCr (< 30) and sFlt-1/PlGF (≤38) results, four groups were described: a double negative result, group A−/−; a negative PCr and positive sFlt-1/PlGF, group B−/+; a positive PCr and negative sFlt-1/PlGF, group C+/−; and a double positive result, group D+/+. The primary outcome was the proportion of false negatives of the combined tests in comparison with PCr alone in the first week after baseline. Secondary, a cost analysis comparing the costs and savings of adding the sFlt-1/PlGF ratio was performed for different follow-up scenarios.ResultsA total of 199 women were included. Pre-eclampsia in the first week was observed in 2 women (2%) in group A−/−, 12 (26%) in group B−/+, 4 (27%) in group C+/−, and 12 (92%) in group D+/+. The proportion of false negatives of 8.2% [95% CI 4.9–13.3] with the PCr alone was significantly reduced to 1.6% [0.4–5.7] by adding a negative sFlt-1/PlGF ratio. Furthermore, the addition of the sFlt-1/PlGF ratio to the spot urine PCr, with telemonitoring of women at risk, could result in a reduction of 41% admissions and 36% outpatient visits, leading to a cost reduction of €46,- per patient.ConclusionsImplementation of the sFlt-1/PlGF ratio in addition to the spot urine PCr, may lead to improved selection of women at low risk and a reduction of hospital care for women with suspected pre-eclampsia.Trial registrationNetherlands Trial Register (NL8308).

Urine creatinine concentration influences the prognostic value of proteinuria for MACE prediction from the findings of the KNOW-CKD study

Proteinuria is typically quantified according to the spot urine protein–creatinine ratio (UPCR) and an association with cardiovascular events has not been thoroughly investigated in chronic kidney disease (CKD) patients. We investigated whether the severity of proteinuria assessed by spot UPCR is associated with an increased risk for cardiovascular outcomes in the CKD population, and whether the relationship is influenced by urine creatinine concentration. We analyzed 1746 patients enrolled as part of The KoreaN cohort study for Outcome in patients With Chronic Kidney Disease (KNOW-CKD). Multivariable Cox proportional hazard analysis was performed to evaluate models with proteinuria as a predictor of renal events and extended major adverse cardiovascular events (eMACEs). Risk for renal events was significantly associated with proteinuria across all eGFR and UPCR categories. By contrast, risk for eMACEs increased significantly with UPCR in patients with eGFR ≥ 60 mL/min/1.73 m2 (hazard ratio [HR] 2.109; 95% confidence interval [CI] 1.375–3.235; P = 0.001), but not in patients with eGFR < 60 mL/min/1.73 m2 (HR 1.086; 95% CI 0.910–1.296; P = 0.358). However, in those with the lower eGFR, risk for eMACEs increased significantly with UPCR in participants with urine creatinine concentration ≥ 95 mg/dL (HR 1.503; 95% CI 1.047–2.159; P = 0.027). In non-dialysis CKD patients, the prognostic value of UPCR for eMACEs is weakened in patients with reduced eGFR levels, for whom it has prognostic significance only in patients with high urine creatinine concentration.

MO372: Concordance Between 24-H Proteinuria and Spot Urine Protein-Creatinine Ratio in the Evaluation of Patients With Glomerular Diseases

Abstract BACKGROUND AND AIMS It has been postulated that the protein-creatinine ratio in spot urine sample (uPCR) could be an adequate alternative to measurement of proteinuria in 24-h urine samples (24-h proteinuria), which despite being the gold standard for the diagnosis and follow-up of patients with kidney disease, its accuracy is frequently influenced by incorrect sample collection. Several studies have shown a correlation between uPCR in spot urine samples and 24-h proteinuria. However, in daily clinical practice we often find significant differences between both measurements. Therefore, we set out to conduct this study to analyze the concordance between uPCR in first morning spot urine samples and 24-h proteinuria in patients with biopsy-proven glomerular diseases. METHOD We identified patients with biopsy-proven glomerular diseases who were evaluated during their disease course at our center between January 1, 2005 and December 31, 2021, and included patients with paired 24-h protein excretion measurement and spot urine samples that measured protein and creatinine concentration, simultaneously collected at the same day. We excluded patients with interstitial or vascular causes for kidney disease. We performed correlation analyses of uPCR and 24-h proteinuria globally and analyzed the influence of demographic data as well as eGFR and type of glomerular disease in this relationship. The concordance between both measurements was compared using intra-class correlation coefficients (ICC) and Bland–Altman plots. RESULTS About 351 patients with simultaneous uPCR and 24-h proteinuria measured at the same day were included in the study, 67.2% were male and the mean age was 53.9 ± 17.9. Median serum creatinine was 1.28 mg/dl (IQR 0.95–1.86) and mean eGFR measured by CKD-EPI was 60.1 ± 30.6 mL/mi/1.73 m2. Median 24-h proteinuria was 1.70 g/day (IQR: 0.62–3.45) and median uPCR was 1.09 g/g (IQR: 0.35–2.70). Wilcoxon rank test showed a systematic underestimation of uPCR compared with 24-h proteinuria (Z = −8.22, P &amp;lt; 0.001). Mean ICC for both measurements was 0.82 (CI 95%: 0.77–0.85, P &amp;lt; 0.001). There was a significant agreement between both measurements after stratifying according to eGFR, with a mean ICC in patients with GFR &amp;gt;60 mL/min of 0.79 (CI 95%: 0.71–0.85, P &amp;lt; 0.001), mean ICC in patients with GFR between 30 and 60 mL/min of 0.80 (CI 95%: 0.72–0.86) and a mean ICC of 0.85 (CI 95%: 0.74–0.91, P &amp;lt; 0.001) in patients with GFR &amp;lt; 60 mL/min. Agreement between Upcr and 24-h proteinuria did not vary according to age, sex or type of glomerular disease. After dividing the cohort into three categories of proteinuria (&amp;lt;1, 1–3, &amp;gt;3 g/day for 24-h proteinuria, and &amp;lt; 1, 1–3, &amp;gt;3 g/g for uPCR), we performed Cohen's κ test to determine agreement, finding moderate agreement between measurements (κ = 0.46, P &amp;lt; 0.001). Passing-Bablok Regression and Bland-Altman plots indicated high concordance between the two methods when proteinuria &amp;lt;3.5 g/day, while outliers in the data suggested greater variability between measurements for patients with proteinuria over 3.5 g/day (Figure 2). CONCLUSION The level of concordance between proteinuria from 24-h urine samples and protein–creatinine ratio from spot urine samples is high regardless of eGFR, age, sex and type of glomerular disease. However, the level of agreement decreases in nephrotic-range proteinuria. Therefore, we suggest that uPCR could be an appropriate measure to assess and follow-up patients with glomerular diseases and non-nephrotic range proteinuria, while the degree of proteinuria should still be monitored with 24-h urine specimens in patients with proteinuria &amp;gt;3.5 g/day.

Working Towards a Treat-to-Target Protocol in Juvenile Proliferative Lupus Nephritis - A Survey of Pediatric Rheumatologists and Nephrologists in Germany and Austria.

BackgroundTo describe treatment practices for juvenile proliferative lupus nephritis (LN) class III and IV of pediatric rheumatologists and nephrologists in Germany and Austria in preparation for a treat-to-target treatment protocol in LN.MethodsSurvey study by members of the Society for Pediatric and Adolescent Rheumatology (GKJR) and the German Society for Pediatric Nephrology (GPN) on diagnostics and (concomitant) therapy of LN.ResultsFifty-eight physicians completed the survey. Overall, there was a considerable heterogeneity regarding the suggested diagnostics and management of juvenile proliferative LN. Increased urinary protein excretion, either assessed by 24 h urine collection or spot urine (protein-creatinine ratio), and reduced estimated glomerular filtration rate were specified as important parameters for indication of kidney biopsy to diagnose proliferative LN and monitoring of therapy. Corticosteroids were generally proposed for induction and maintenance therapy, most often in conjunction with either mycophenolate mofetil (MMF) or cyclophosphamide (CP) as steroid-sparing immunosuppressants. MMF was clearly preferred over CP for induction therapy of LN class III, whereas CP and MMF were equally proposed for LN class IV. MMF was most often recommended for maintenance therapy in conjunction with oral corticosteroids and continued for at least 3 years and 1 year, respectively, after remission. Hydroxychloroquine was widely accepted as a concomitant measure followed by renin-angiotensin system inhibitors in cases of arterial hypertension and/or proteinuria.ConclusionThe majority of pediatric rheumatologists and nephrologists in Germany and Austria propose the use of corticosteroids, most often in combination with either MMF or CP, for treatment of proliferative LN in children. The considerable heterogeneity of responses supports the need for a treat-to-target protocol for juvenile proliferative LN between pediatric rheumatologists and nephrologists.

Prevalence of Infection and Changing Pattern of Organisms Causing Infections in Childhood Nephrotic Syndrome

Background: Infection remains an important complication of children with nephrotic syndrome. It results in significant morbidity and may also be responsible for a poor response to steroid therapy or induce relapse in child who has already attained remission.&#x0D; Objectives: This study was conducted to find out pattern of infection and type of organisms causing infections in nephrotic syndrome.&#x0D; Methods: This cross sectional study was conducted in the Paediatric Nephrology Department of Dhaka Shishu (Children) Hospital from January 2010 to November 2010. One hundred fifteen (115) cases of nephrotic syndrome, age between 1 to 13 years were enrolled according to the inclusion criteria. Along with routine investigations, urine, blood and throat swab culture and sensitivity and MT test were done. Risk factors of infection were also determined. Statistical analysis was done by SPSS version 12. Level of significance was taken as &lt;0.05.&#x0D; Results: Prevalence of infection in nephrotic syndrome was 54.78%. Infections were more common in childhood nephrotic syndrome below 6 years of age. Infections encountered in nephrotic syndrome were UTI 51(44.34%), septicemia 4(3.47%), pneumonia 5(4.34%), peritonitis 1(0.87%), cellulitis 1(0.87%) and tuberculosis 1(0.87%). Statistically significant risk factors associated with infection were generalized edema, steroid dependence, steroid resistance, persistent proteinuria and high spot urine protein creatinine ratio. In case of UTI E. coli was the commonest 27(52.9%) organism followed by Morganella and Pseudomonas 5(9.8%).&#x0D; Conclusion: Prevalence of infection in nephrotic syndrome is very high and E. coli is the commonest organism found in this study. Generalized edema, persistent proteinuria, hypoalbuminemia, steroid dependence, steroid resistance are important risk factors for infection.&#x0D; DS (Child) H J 2021; 37(1): 28-33

Comparative Evaluation of Quantitative Protein Measurement of 12-Hour and 24-hour Urine Sample for the Diagnosis of Nephrotic Syndrome and their Correlation with Spot Urinary Protein Creatinine Ratio

Background: The amount of protein excretion is a reflection of disease activity in Nephrotic Syndrome. 24-hour urine collection has remained as the method of choice to quantify proteinuria. An alternative method for quantitative evaluation of proteinuria is measuring the ratio of protein or albumin to creatinine in an untimed ‘‘spot’’ urine specimen.&#x0D; Objective: To compare 12-hour versus 24-hour proteinuria in nephrotic syndrome and its correlation to spot urinary protein creatinine ratio.&#x0D; Materials &amp; Methods: A comparative cross-sectional study was conducted in the Department of Pediatrics, Sir Salimullah Medical College Mitford Hospital (SSMC &amp; MH), Dhaka over a period from September 2016 to June 2017. All the babies developing 1st attack of Nephrotic Syndrome and meeting the clinical criteria were included in the study. Complete history was taken from accompanying attendants. Thorough clinical examinations and relevant laboratory investigations (12- hour day urinary protein, 12-hour night urinary protein, 24-hour urinary total protein, S. Albumin, S. Cholesterol, Urinary spot Protein Creatinine ratio) were done. All the information was recorded in the fixed protocol. Collected data were classified, edited, coded and entered into computer for statistical analyses.&#x0D; Results: Out of 50 cases, the mean age was found 58.3±29.0 months. 12-hour day urinary protein was found 2.02±0.167 gm/m2/12 hours, mean; 12-hour night urinary protein was found 2.12±2.18 gm/m2/12 hours, mean; 24-hour urinary total protein was found 4.10±3.32 gm/m2/24 hours. The mean spot urinary protein creatinine ratio was found 12.58±7.21 with range from 2.02 to 29.85. There was no statistically significant difference between 12- hour day sample and 12-hour night sample. There was also no significant difference between 12-hour day &amp; night sample with 24-hour sample. Results of all 03 samples were comparable to urinary protein creatinine ratio.&#x0D; Conclusion: This study reveals no statistically significant difference in quantitative estimation of 12-hour day and night protein with 24-hour urinary total protein. The 12- hour day and night protein and urinary total protein were comparable with each other and also with urinary spot protein creatinine ratio.So 12-hour day or night urinary total protein may be a diagnostic tool for nephrotic syndrome.&#x0D; Sir Salimullah Med Coll J 2021; 29(2): 105-111

Comparing Spot Urine Protein: Creatinine Ratio 24-Hour Urinary Protein Estimation in Type 2 Diabetes Mellitus Patients

Background &amp; Objective: A simple blood test (urea and creatinine) and a urine test may indicate renal function deterioration and presence of microalbuminuria, which is also the first clinical signs of renal dysfunction in patients with diabetes mellitus. This cost effective test easy to perform even in the absence of advance facilities in a hospital. That is why we planned to conducted this study to assess the comparison between of spot urine protein:creatinine ratio with 24-hour urinary protein in patients with type 2 diabetes mellitus.&#x0D; Materials and Methods: This prospective hospital based clinical study was conducted in the Department of General Medicine, Liaquat University of Medical &amp; Health Sciences, Jamshoro, over a period of six months from 10th May 2018 to 9th November 2019 through a consecutive sampling technique. All the patients having age more than 18 years of both gender, and type 2 diabetes mellitus were enrolled in this study. Three cc blood was taken to determine the serum creatinine levels and twenty-four hour 2ml urine sample was also collected to determine the urinary protein levels. The proteinuria ≥300mg/dl in 24-hour urine sample was considered as significant proteinuria. Kappa statistics was used to find agreement between spot urine protein and 24 hours urinary protein.&#x0D; Results: A total 95 patients were evaluated and their mean age was 41.91±14.29 years, with male predominance (n = 66, 69.4%). Average 24 hour urinary protein was 1216.99±949.51mg and spot-urine evaluation of protein was 1919.12±2129.25mg. The agreement between spot urinary protein creatinine ratio and 24 hour urinary protein was found in 82.1% of cases through Kappa statistics and the calculated agreement between the two procedures was 0.975 which provides sufficient agreement to use spot urine protein:creatinine ratio in routine diagnosis of proteinuria.&#x0D; Conclusion: The study have shown that the protein:creatinine ratio for a random urine sample might be used to rule out the presence of significant proteinuria as defined by a quantitative measure of the 24-hour urine protein excretion.

Frequency of kidney dysfunction in patients with acute stroke and the relationship with the type, severity and outcome.

Kidney dysfunction is an established risk factor for cardiovascular diseases including stroke. The study aimed at assessing the frequency of kidney dysfunction in patients with acute stroke and to evaluate the relationship to the type, severity and outcome of stroke. To establish a relationship, which has not been explained in past studies. This was a cross-sectional analytical study on acute stroke patients and matched controls, evaluating for kidney dysfunction using both estimated glomerular filtration rate (GFR) and the spot urine protein creatinine ratio. The type of stroke was observed by neuroimaging. The National Institute of Health Stroke Score was used to assess the severity of stroke at presentation and outcome after 7 days. Data analysis was done using Statistical Package for Social Sciences (SPSS) application version 23.0 (SPSS Inc., Chicago, IL, USA). : Ninety-eight patients and 100 controls were recruited, with a mean age of 64.7 ± 15.5 and 64.8 ± 15.1 years, respectively. The patients with stroke had a statistically significant higher frequency of kidney dysfunction compared to the controls (85.9% vs. 62.0%, P ≤ 0.001). Patients with haemorrhagic stroke had a higher frequency of kidney dysfunction compared with those with ischaemic stroke (93.8% vs. 77.3%, P = 0.048). The proportion of patients with kidney dysfunction was seen to increase from those with mild to those with severe stroke symptoms, both at presentation and after 7 days. Estimated GFR was seen to be an independent predictor of poor outcome in patients with stroke (odds ratio 0.955, 95% confidence interval 0.924 - 0.986, P = 0.005). The study demonstrated that in patients with acute stroke there is a high frequency of kidney dysfunction. Haemorrhagic stroke, increasing stroke severity and poor outcome were seen to be associated with kidney dysfunction. Thus, recommending the need for kidney care as an important part of stroke management.