Repeated sprint ability (RSA) is crucial for success in team sports, and involves both neuromuscular and metabolic factors. While single-mode training (SGL; e.g., sprint training) and combined training (CT; e.g., sprint + plyometric) can improve RSA, whether CT offers additional benefits compared to SGL or active controls maintaining routine training (CON) remains uncertain in team-sport athletes. This study evaluates the effect of CT versus SGL and CON on the RSA of team-sport athletes. A comprehensive search was conducted in five electronic databases. Thirteen studies involving 394 males and 28 females, aged 14 to 26 years, were included. The random effects model for meta-analyses revealed greater improvement in RSA mean after CT compared to SGL (Hedge's g effect size [g] = -0.46; 95 % confidence interval [CI]: -0.82, -0.10; p < 0.01) and CON (g = -1.39; 95% CI: -2.09, -0.70; p < 0.01). CT also improved RSA best compared to CON (g = -1.17; 95% CI: -1.58, -0.76; p < 0.01). The GRADE analyses revealed low- to very-low certainty of evidence in all meta-analyses. Subgroup analysis revealed that plyometric + sprint training yielded greater RSA mean (g = -1.46) and RSA best (g = -1.35) improvement than plyometric + resistance + sprint training and resistance + sprint training. The effects of CT on RSA did not differ according to age (≥ 18 vs. < 18), sports (e.g., soccer vs. basketball vs. handball), or RSA test type (linear sprint vs. sprint with change-of-direction). Studies showed an overall high risk of bias (ROB 2). In conclusion, CT may be improving team-sport athletes' RSA more effectively than SGL (small effect size) and CON (large effect size), particularly when CT involves plyometric + sprint training.
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