Spontaneous Succession Research Articles

State of the population of Pulsatilla patens (L.) Mill. on the territorу National Nature Park «Podilski Tovtry»

The population of Pulsatilla patens has been described in the NNP «Podilski Tovtry» national nature park on the territory of the «Ivakhnovetskiy» protected area. The article analyzes the data from the literature on the possible systematic position of P. patens and its range. Literary sources and herbarium specimens stored in local herbaria were processed, and probable localities in the territory of the national park were described. The only place of growth on the «Ivakhnovetskiy» of the NNP «Podilski Tovtry» has been confirmed. The ecological and coenotic relationships are characterized, and the biological features of the studied species are discussed. The geobotanical descriptions within the «Ivakhnovetskiy» protected area are carried out. The habitats and communities from the Green Book of Ukraine with P. patens were identified. The physical and geographical features of the habitat of the studied species are characterized. The results of statistical processing of morphometric parameters of P. patens, which were analyzed within the NNP «Podilski Tovtry», are processed and presented. It was found that the population on the «Ivakhnovetskiy» is predominantly homogeneous. The largest coefficient of variation was determined for the number of flowers and the height of the pedunculate shoot, which is due to the biological characteristics of the species. Based on the results of accounting for the age structure of the P. patens population in this locality, it was found that the recovery index is moderate. The factors of influence on the population of Pulsatilla patens on the «Ivakhnovetskiy» were determined. The conservation measures for the restoration and conservation of the rare species P. patens in the territory of NNP «Podilski Tovtry» and in Ukraine in particular at the eastern limit of its distribution are proposed. It is proposed to propagate the germinated seeds at the collection site with further return to the natural habitat of «Ivakhnovetskiy» and other localities identified during the study of literary and herbarium materials – to reintroduce it. Controlled spontaneous succession in abandoned and/or still active limestone quarries with large areas devoid of dense vegetation can be an effective tool for population recovery.

Mosaic of biological soil crusts and vascular plants contributes to the spatial heterogeneity of key soil properties at different successional stages of restored inland sand dunes

Abstract Background and aims Inland sand dunes constitute Natura 2000 habitat that has become a priority to ensure biodiversity protection and habitat heterogeneity at the landscape scale; however, without proper management, spontaneous succession leads to overgrowing of trees and thus to habitat degradation. Understanding succession processes, relationships between biotic and abiotic components, and their changes over time after restoration, is the key to proper planning of future restoration activities. The aim was to determine the changes of biological soil crust (BSC), vascular plants and physicochemical parameters of BSC, below-crust, rhizosphere, and bare substrate types at different stages of succession in inland dunes of the Błędowska Desert (Poland). We also analyzed the interplay between these factors to further understand the mechanism of BSC development and recognize driving factors causing changes in the soil environment. Results Our results showed that BSCs contributed to increased organic C, total N, nutrients in soil, and acidification with the succession. The content of inorganic N forms was significantly higher in bare soil compared to below-crust soil. Rhizosphere soil was enriched in organic matter and nutrients, which improves soil conditions within plant patches. Moreover, below-crust soil pH, total N content and water holding capacity drive the patterns of BSC morphological groups and species composition of lichens, bryophytes, and vascular plants. Conclusion Our study shows that spatial structure of the inland dune ecosystem is a mosaic of BSCs and plants that contribute to the spatial heterogeneity of key soil properties. We concluded that it is necessary to consider the matter of BSC in restoration treatments.

Assessing the Effectiveness of Rotation Growing of Industrial Hemp and Alfalfa in Post-Mining Agricultural Reclamation: Using Soil Fauna as an Indicator

The reclamation of post-mining land for agricultural purposes has continued to be a big challenge. Our study concerns the use of soil microfauna (nematodes) and mesofauna (mites and springtails) as indicators of soil quality after 6 years of agricultural reclamation of a post-mining area in west–central Poland. A new method, which involves rotation growing of industrial hemp (H) and alfalfa (A) and incorporating the resulting biomass into the soil, was used to reclaim two sites (5 and 15 years after mining) representing different types of post-mining deposits (clayey and sandy). On each site, two plots were established, where each crop was grown for three years, but in a different order during the rotation cycle (3H3A and 3A3H). The results showed significant differences in the abundance and structure of the fauna communities between 3H3A and 3A3H reclamation practices, as well as between the reclaimed plots and non-reclaimed (NR) plots, where spontaneous succession proceeded. The three animal groups were more abundant in the reclaimed soil compared to the NR soil. The highest densities for nematodes were observed in the 3H3A plots and for the mesofauna in the 3A3H plots. The reclamation practices had a positive effect on groups involved in the regulation of C and N mineralisation, particularly bacterial- and hyphal-feeding nematodes and oribatid mites, and a negative effect on plant-feeding nematodes and euedaphic collembolans. The finding that most of the parameters of the studied biota had values resembling those of agricultural soils after 6 years of reclamation clearly indicates the effectiveness of the applied practices for transforming degraded land into soils that mirror soils under agricultural use.

Scale dependency of taxonomic and functional diversity in pristine and recovered loess steppic grasslands

Widespread campaigns on forest restoration and various tree planting actions lower the awareness of the importance of grasslands for carbon sequestration and biodiversity conservation. Even lower attention is given to the conservation of biodiversity and ecosystem functioning in remnants of ancient, so-called pristine grasslands. Pristine grasslands generally harbour high biodiversity, and even small patches can act as important refuges for many plant and animal species in urbanised or agricultural landscapes. Spontaneous succession of grassland is frequently viewed as a cost-effective tool for grassland restoration, but its applicability is strongly dependent on many local to landscape-scale factors, and the recovery is often slow. It is therefore essential to monitor the success of grassland restoration projects that rely on spontaneous succession. We compared the species diversity and functional attributes of pristine and recovered grasslands by studying the taxonomic and functional diversity in thirteen (8 pristine and 5 recovered) loess steppic grasslands using differently sized sampling plots from 0.01 to 100 m2. Our results indicate that there are remarkable differences in taxonomic and functional diversity between pristine and recovered grasslands. We also found that during secondary succession there is a likely functional saturation of the species assembly in the first few decades of recovery, and while patterns and structure of recovered grasslands became quite similar to those of pristine grasslands, species richness and diversity still remained much lower. Pristine grasslands support considerable plant diversity, and species composition is slow to recover if destroyed by agricultural land use. This underlines the importance of preserving existing pristine grassland remnants, which might serve as sources of species for future restoration measures.

Soil bacterial activity and functional diversity as indicators of recultivation of alkaline settlements of a ‘Solvay’ process

PurposeMany industries continuously produce large volumes of alkaline residues. After recultivation, former landfills can be an important element providing ecosystem services in highly urbanised areas.Materials and methodsWe studied the relationship between vegetation and soil bacterial characteristics using Biolog® ECO plates on recultivated areas of the Solvay heaps, and participation of invasive species in plant community in progressive succession.Results and discussionBacterial activity and functional diversity indices were correlated with soil physicochemical properties (soil organic matter content, water holding capacity, total phosphorus content, and soil pH). Plots with a higher total number of vascular plant species, including forest floor species, and a higher Shannon–Wiener index of vegetation diversity H'plant, were characterised by a higher amino acid utilisation by soil bacteria. Soil bacteria CLPP (community-level physiological profiles) were most influenced by soil physical and chemical properties, but also by the proportion of invasive species in the total number of plant species. In conclusion, soil physical and chemical properties dominate vegetation characteristics in shaping the functional diversity of soil bacteria. Invasive species may be an influential component of ecosystems during spontaneous succession of degraded soils.

Fires in the face of climate change: Indicators of fire disturbance in heath areas – Inference from military training lands

Global warming significantly affects the frequency and intensity of wildfires in many fire-prone areas of the world and fire disturbance regimes are changing rapidly. Alongside this, controlled burning is often considered or implemented as an alternative method for nature protection. Here, we studied the post-fire secondary succession in dry heath habitat located in military training grounds to recognise the impact of fire on the effectiveness and rate of ecosystem recovery. We focus particularly on identifying indicator species for a given successional phase of Central European dry heath habitats and assessing their value for dating the last fire disturbance. The research involved 60 vegetation patches (plots of 25 m2), which were assigned to four post-fire age classes, namely: 1–5 years (Class A), 6–10 years (Class B), 11–15 years (Class C), and >15 years (Class D). In each study plot, species diversity and coverage of lichens, bryophytes and vascular plants were examined in addition to the physicochemical properties of the soil substrate. Cryptogams and vascular plants clearly differ in terms of the secondary succession pattern; specific sets of cryptogams correspond well to particular post-fire classes and are therefore good determinants of the post-fire succession stage. Spontaneous succession of plant vegetation eventually leads to complete recovery of the heath in a relatively short time. Nevertheless, great vegetation dynamics in the first years after a fire disturbance may result in seemingly different directions of succession. Post-fire classes did not differ noticeably in terms of soil properties, it follows that the effects of fire on soil conditions are negligible; though, a significant upward trend was observed for exchangeable form of K throughout the succession process. Our results indicate that sporadic fires reduce the undesirable overgrowth of heathlands or psamophilous grasslands and generally have a little negative side-effect on the ecosystem. The revealed succession patterns and defined sets of species characteristic for subsequent post-fire age classes are applicable to dating fire disturbances, regardless of whether the fire was planned or spontaneous.

Influence of Fly Ash on Soil Properties and Vegetation of Fresh Coniferous Forest during Long-Term Observation

Fly ashes produced in huge amounts during coal combustion requires proper management. The purpose of this study was to determine the impact of fly ash from burning hard coal used in large doses (250, 500, 1000 and 2000 t ha−1) on soil properties and vegetation of fresh mixed coniferous forest within 43 years from the ash application. The experiment was established in the Podzols in the forest habitat of Czułów, Katowice Forest district, Upper Silesia, Poland. Eight tree species were planted in ridges created by ploughing: Pinus sylvestris, P. nigra, Larix decidua, Betula pendula, Quercus robur, Q., Acer pseudoplatanus and Fagus sylvatica. The changes in soil morphology caused significant transformations in the physical and chemical properties of the soil such as soil texture, pH, macronutrients (P, K and Mg) content and C:N ratio. Increasing of ash doses changed the granulometric composition of the soil levels from loamy sand (250 t/ha−1) to silt loam (2000 t ha−1). Initially, the acidic Podzols were alkalized under the influence of the fly ash and then acidified, possibly due to the impact of accumulated litter layers, and the reaction of organic soil horizons changed from strongly acidic (250–1000 t ha−1) to alkalis (2000 t/ha−1). The macronutrients content increased in proportion to the fly ash dose, but the subsequent acidification resulted in a gradual decrease in the macronutrients share in the soil layers. The value of the C:N ratio grew after the ash application and then it gradually reduced, even by half. The transformations of soil horizons’ properties also increased the capacity of the soil sorption complex (CEC). All these processes led to a change in the trophic status of the habitat expressed by the soil habitat index (SIG) and the initial coniferous forest site can be classified as a mixed forest habitat even with the lowest ash dose used. The composition of plant communities developed forty years after the ash application was similar at the lower ash doses and the most frequent and abundant tree species were L. decidua, P. nigra and P. silvestris. B. pendula was previously co-dominant, but it was eliminated from the tree stands during the experiment. Planted trees characteristic of late stages of succession, such as Q. robur, Q. rubra, F. sylvatica and A. pseudoplatanus either did not survive or remained in very low quantities. The herb and moss layers developed in the process of spontaneous colonization, and together with the trees led to phytostabilisation of the bare substrates. After acidification of the topsoil horizons, the herb layers consisted mostly of coniferous, mixed, and deciduous forest species, and the most frequent or abundant were Lysimachia europea and Pteridium aquilinum. The moss layers were represented by coniferous forest flora. At the ash dose of 2000 t ha−1, Tilia cordata settled in one of the seral stages of spontaneous succession and this species dominated in the community and formed a dense tree stand. After the soil acidification, a shift from calcicole to calcifuge plant strategy took place among species of the herbaceous layer. The transformations of plant communities’ composition occurred in relation to changes in the soil properties.

Changes in soil microbe-mediated carbon, nitrogen and phosphorus cycling during spontaneous succession in abandoned Pb[sbnd]Zn mining areas

The mechanism through which soil microorganisms mediate carbon and nutrient cycling during mine wasteland restoration remained unknown. Using soil metagenome sequencing, we investigated the dynamic changes in soil microbial potential metabolic functions during the transition from biological soil crusts (BSC) to mixed broad-conifer forest (MBF) in a typical PbZn mine. The results showed soil microorganisms favored carbon sequestration through anaerobic and microaerobic pathways, predominantly using efficient, low-energy pathways during succession. Genes governing carbon degradation and aerobic respiration increased by 19.56 % and 24.79 %, respectively, reflecting change toward more efficient and intensive soil carbon utilization in late succession. Nitrogen-cycling genes mediated by soil microorganisms met their maximum influence during early succession (sparse grassland, SGL), leading to a respective increase of 75.29 % and 76.81 % in the net potential nitrification rate and total nitrogen content. Mantel and correlation analyses indicated that TOC, TN, Zn and Cd contents were the main factors affecting the soil carbon and phosphorus cycles. Soil AP content emerged as the primary influencer of genes associated with the nitrogen cycle. These results shed light on the dynamic shifts in microbial metabolic activities during succession, providing a genetic insight into biogeochemical cycling mechanisms and underscoring crucial factors influencing soil biogeochemical processes in mining regions.

Assembly of heterotrophic communities during spontaneous succession in quarries: invertebrates model groups and macromycetes

Quarrying has a crucial impact on the environment, but it could enhance species diversity. Mining sites represent important refuges for countless species disappearing from homogenous landscapes. Our study focused on assemblages of heterotrophic communities such as moths (Lepidoptera), carabid beetles (Coleoptera: Carabidae), spiders (Araneae), and macromycetes (fungi: Basidiomycota, Ascomycota) in an active part of kaolin quarries and their immediate surroundings in the Pilsen region, Czech Republic. We compared differences between mined and unmined sites, sites with spontaneous succession and sites with replanted pine trees. In total, we recorded 178 moth, 63 spider, 27 carabid beetle, and 81 macromycetes species, including 21 Red‐listed species. The moths, carabid beetles, and macromycetes tended to inhabit unmined sites; on the contrary, open habitat spiders preferred open sites with replanted pine trees. Based on the life history traits analyses, moth species feeding on forbs and grasses prevail at the active part of kaolin quarries, where higher plant diversity was detected. Large body carabid beetles such as Carabus spp. favored unmined sites, as well as macromycetes with long‐lived fruit bodies. Open habitat and xerophilous spiders inhabited the replanted sites by pine trees where the sparse vegetation was obvious. Our results indicated that groups with radically different life histories such as moths, carabids, and macromycetes may react to mining remarkably similarly, although spiders, despite sharing predatory habits with the majority of carabids, reacted differently.

Active versus passive restoration of tallgrass prairie in the U.S. Midwest: plant species diversity and assemblage, net primary production and soil carbon sequestration

We investigated the plant species diversity, community assemblage, net primary production and soil carbon sequestration in an old field (OF), which went through passive restoration with spontaneous secondary succession, and a restored prairie (RP), which went through active restoration with reintroduction of native plants. The passive restoration in OF did not attain as high diversity and richness of plant species as did in RP. Our NMS (nonmetric multidimensional scaling) ordination revealed a clear divergence of RP and OF vegetation with dissimilar species compositions to separate paths of succession trajectory. The spontaneous succession in OF has made very little progress due to a potentially strong inhibitory priority effect of invasive plants. The belowground biomass in the high‐diversity RP was 1.3× higher than the low‐diversity OF. We found no measurable difference in soil C sequestration between RP and OF at this time, possibly due to edaphic factors, lack of legumes, or both. Human intervention is needed for restoring abandoned croplands—highly disturbed, fragmented and isolated habitats—to tallgrass prairies in the U.S. Midwest. Our recommended intervention includes but is not limited to removal of exotic plants, further augmentation of native plants (particularly legumes), and periodic burns.

How do root fungi of Alnus nepalensis and Schima wallichii recover during succession of abandoned land?

Alnus nepalensis and Schima wallichii are native tree species accompanying succession in abandoned agricultural land in the middle mountainous region of central Nepal. To understand how root fungi recover during spontaneous succession, we analyzed the diversity and composition of arbuscular mycorrhizal (AM), ectomycorrhizal (ECM), and total fungi in tree fine roots from three land use types, short-term abandoned land (SA), long-term abandoned land (LA), and regenerated forest (RF) as a reference. Additionally, ECM morphotypes were examined. The results showed different speeds of succession in the studied fungal groups. While the change in the AM fungal community appears to be rapid and LA resembles the composition of RF, the total fungi in the abandoned land types are similar to each other but differed significantly from RF. Interestingly, the relative abundance of Archaeosporaceae followed a trend differing between the tree species (SA < LA in A. nepalensis, but SA > LA in S. wallichii). Unlike AM and total fungi, there was no significant difference in the ECM community of A. nepalensis between land use types, probably due to their low species diversity (9 ECM morphotypes, 31 ECM operational taxonomic units). However, Cortinarius sp. was significantly more abundant in RF than in the other land use types, whereas Alnicola, Tomentella, and Russula preferred young stages. Our results suggest that for both studied tree species the AM fungal succession could reach the stage of regenerated forest relatively fast. In the case of total fungi, because of hyperdiversity and composed of species specialized to a variety of environments and substrates, the transition was expected to be delayed in abandoned land where the vegetation was still developing and the ecosystem was not as complex as that found in mature forests.

Intense game grazing shapes epigeic spider communities of pedunculate oak Quercus robur plantations in anthropogenic environments

Game browsing affects nature conservation because the interests of hunters and gamekeepers often lead to high game stocking rates. However, the compositional effects of game browsing on invertebrates are insufficiently understood. We addressed the dynamics of epigeic spider communities in oak stands planted in newly formed anthropogenic environments under the facultative pressure of browsing game. The study site consisted of an open-cast lignite mine and an overburden deposit in NW Czechia. Using pitfall traps, we examined five habitat types (four transects per habitat type): Quercus robur stands ≥ 25 years of age, 12–24 years of age, and 0–2 years of age; Q. robur stands 12–24 years of age that were heavily damaged by game browsing; and sites subject to spontaneous succession for 12–22 years. We captured 9459 individuals from 134 spider species distributed unevenly across the examined habitat types. The young stands were dominated by Pardosa agrestis, Pardosa monticola, and Oedothorax apicatus, whereas Pardosa prativaga, Pardosa lugubris, Pisaura mirabilis, and Alopecosa cuneata dominated the old stands. The stands damaged by browsing had the highest species richness and were dominated by Alopecosa pulverulenta, Xerolycosa miniata, A. cuneata, and Trochosa ruricola. All the oak stands were rich in threatened species, with most (26) found in habitats damaged by browsing, whereas the youngest stands hosted the lowest number of threatened species (11). The species dominating old stands still included species that prefer greater canopy openness, such as P. lugubris, whereas numerous species of central European climax oak forests were lacking. In conclusion, we identified oak stands heavily damaged by game browsing as a unique environment that shapes the community of epigeic spiders and that differs from epigeic spider communities of oak stands of various ages and those colonizing areas that are allowed to undergo spontaneous succession.

Woody species succession and spontaneous forest development in post-mining sites after coal mining in the Czech Republic

There is little information about long-term spontaneous forest development after industrial disturbances. A field study was done along the chronosequence of unreclaimed post-mining sites (12, 25, 32, 60 and 90 years old), developing by spontaneous succession in the northwestern Czech Republic. Initial stages of forest development (12- to 60-yr-old) were dominated mainly by silver birch (Betula pendula) goat willow (Salix caprea) and aspen (Populus tremula), Norway spruce (Picea abies) also established naturally in the intermediate stages of succession. A 90-yr-old site was close to the expected climax forest with 21 woody species dominated by European oak (Quercus robus L.), supplemented by beech (Fagus sylvatica). The youngest and oldest sites were more diverse and richer sites in woody species than others, as expressed by the Shannon-Weiner and Simpson indices. Conversely, spatial heterogeneity (aggregation) increased with increasing age; however, it became lower at the age of 90 years. Woody species in the understory were more diverse than in the tree layer. Overall, our study found that spontaneous processes can lead to the development of the late-successional forest in fewer than 100 years in Central Europe.

Influence of Distance from Forest Edges on Spontaneous Vegetation Succession Following Small-Scale Gold Mining in the Southeast Peruvian Amazon

Few studies describe the factors that influence the natural regeneration in abandoned gold mining areas in the Amazon. Here we focus on the influence of the distance to the forest edge and abandonment time in a spontaneous succession of degraded areas by gold mining in the southeastern Peruvian Amazon. We assessed woody species composition (DBH ≥ 1 cm) and forest stand structure across a chronosequence (2–23 years). A total of 79 species belonging to 30 families were identified. The natural regeneration was dominated by Fabaceae, Malvaceae, and Urticaceae. Together, they represented 60% of the importance index. Cecropia membranacea and Ochroma pyramidale were the dominant pioneer species at the initial successional stage. The basal area and species diversity were directly related to time after abandonment and inversely related to the distance to forest edges. The distance-based redundancy analysis showed that more of the variation in species composition was explained by distance to the forest edge than the abandonment time. Our study revealed that regeneration was relatively slow and provided evidence that the distance to the forest edge is important for natural regeneration in areas degraded by gold mining.

Detecting long-term effects of mining-induced ground deformation on plant succession in semi-arid areas using a cellular automata model

Mining-induced ground deformation affects various plant communities in different ways. However, little is known about the effects of ground deformation on plant succession; no known quantitative approach currently exists to detecting the long-term effects. To address this issue, a plant community succession model based on cellular automata was developed. Plant succession over 30 years within a subsidence area in the Yungang mining area in China was simulated and compared under mining and non-mining scenarios, including under two sets of initial conditions (vegetated and bare). The normalized mean square error was applied to test the accuracy of the plant community succession model and to reveal the effects of ground deformation at the patch scale, which was 0.06–0.59 for simulated and observed plant patterns. Landscape indicators including contagion, patch cohesion, interspersion and juxtaposition, Shannon’s diversity, Shannon’s evenness, and aggregation indices were calculated and compared to reveal the effects of mine subsidence at the landscape scale. The results showed that the interspersion and juxtaposition, Shannon’s diversity, Shannon’s evenness, and aggregation indices were significantly influenced by ground deformation (P < 0.05) under vegetated conditions, indicating that ground deformation resulted in a loose, isolated, and mixed pattern of vegetation. In terms of the shrub and tree communities, the normalized mean square errors fluctuated at a high level under bare conditions, especially at the patch scale of 10 × 10 m, where it remained at greater than 0.5. Overall, mining-induced ground deformation had long-term effects on plant succession by limiting tree communities and promoting shrub communities, as well as by leading to a heterogenous vegetation pattern. Ground deformation altered the topography of the previously mined area, influencing hydrological processes such as rainfall, infiltration, and evaporation, which were closely related to plant succession. Although these long-term effects were not directly a result of ground deformation, changes in the topography of a subsidence area would interfere with the spontaneous succession of plant communities. Therefore, the long-term effects of mining-induced ground deformation should be considered during the ecological restoration of a mining area, which can promote the application of nature-based solutions and the natural regeneration of plant communities.

The interactive effect of reclamation scenario and vegetation types on physical parameters of soils developed on carboniferous mine spoil heap

AbstractBulk density, porosity, and water retention capacity play a key role in limiting root growth and nutrient uptake in developed technogenic soils. Thus, the purpose of this study was to evaluate the impact of reclamation technologies and different vegetation types on vital physical soil properties formed from carboniferous materials. The case study was conducted on Sośnica hard coal post‐mine spoil heaps (Upper Silesian Coal Basin of Southern Poland). Nine experimental combinations of reclamation technologies and vegetation types were tested. For vegetation types and basic physical soil parameters, 120 plots (10 × 10 m) were selected. The texture analysis was done on composite soil samples collected at two uppermost soil depths (0–10 and 10–20 cm). Intact structure samples were collected into 100cm3 cylinders from the middle of each plot at both depths for bulk density (BD), porosity, capillary water capacity (CWC), and soil water storage (SWS). In the comparison of different reclamation scenarios, we noted that topsoiling increased BD, whereas CWC and porosity decreased. Spontaneous succession had the same effect on BD, porosity, and CWC as cultivation. Revegetation of the spoil heap with grasses and forbs resulted in lower BD and higher porosity and CWC in the top layer (0–10 cm), whereas woodland resulted in lower BD and higher porosity and CWC at lower depths (10–20 cm). The decreasing order of the SWS was grassland &gt; forbland &gt; woodland. Thus, forbs and grasses should be followed by tree planting, which is crucial to improving the hostile physical properties of the Carboniferous postmine soils.

A review of methodology for grassland restoration and management with practical examples

Currently, the majority of high nature value Slovenian grasslands have an unfavourable conservation status. Based on the available data from habitat type mappings, the surface of high nature value grasslands (6210(*) – semi-natural dry grasslands and scrubland facies on calcareous substrates, 6410 – Molinia meadows, and 6510 – lowland hay meadows) at Natura 2000 sites is decreasing. The existing agri-environment measures have been only partly effective in promoting grassland biodiversity. The main threats to biodiversity are driven by various anthropogenic activities, which result in a continuous change in landscape identity, habitat fragmentation, and ecosystem degradation. Therefore, biodiversity restoration became an urgent step in conservation of high nature value grasslands. Multiple pathways may lead to the ecological restoration of grasslands with an altered, uncharacteristic floristic composition, or grasslands with an unfavourable conservation status. In this paper, we present an overview of the methods used in the restoration of grasslands from different parts of Europe. In an adapted form, these methods could also be used for the restoration of Slovenian grasslands. Grasslands may be left to spontaneous succession, which is mainly suitable for small-scale areas, located in the proximity of grasslands with well-preserved biodiversity. However, to re-establish plant communities with specialist species, a more proactive approach is usually required, such as sowing of regional or commercial seed preservation mixtures, transfer of mature plant material, or topsoil transfer from donor sites with appropriate botanical composition. Grassland restoration methods should be carefully thought-out and carried out before the habitat or species is endangered. We conclude that optimally chosen post-restoration management may have an impact that is comparable or even greater than the impact of a suitable restoration method. Nevertheless, maintenance of well-preserved grasslands is still much more cost-effective than restoration of degraded grasslands.

Spontaneous Primary Succession and Vascular Plant Recovery in the Iberian Gypsum Quarries: Insights for Ecological Restoration in an EU Priority Habitat.

Gypsum covers a vast area of the Iberian Peninsula, making Spain a leader in its production. Gypsum is a fundamental raw material for modern societies. However, gypsum quarries have an obvious impact on the landscape and biodiversity. Gypsum outcrops host a high percentage of endemic plants and unique vegetation, considered a priority by the EU. Restoring gypsum areas after mining is a key strategy to prevent biodiversity loss. For the implementation of restoration approaches, understanding vegetation's successional processes can be of invaluable help. To fully document the spontaneous succession in gypsum quarries and to evaluate its interest for restoration, 10 permanent plots of 20 × 50 m were proposed, with nested subplots, in which vegetation change was recorded for 13 years in Almeria (Spain). Through Species-Area Relationships (SARs), these plots' floristic changes were monitored and compared to others in which an active restoration was carried out, as well as others with natural vegetation. Furthermore, the successional pattern found was compared to those recorded in 28 quarries distributed throughout the Spanish territory. The results show that an ecological pattern of spontaneous primary auto-succession is widely recurring in Iberian gypsum quarries, which is capable of regenerating the pre-existing natural vegetation.

A comment on “International principles and standards for the ecological restoration and recovery of mine sites”—useful but limited

The Principles and Standards by Young et al. provide an international framework for ecological restoration of mine sites. Although useful, these Standards are limited as a basis for practice, especially in Europe, partly by neglecting the fact that semi‐natural habitats are often desirable restoration targets in anthropogenic cultural landscapes; intermediate and early successional stages are sometimes more ecologically valuable than terminal stages; spontaneous succession can be an effective restoration approach, or it may be combined with assisted restoration; and by a limited number of representative long‐term restoration case studies. Further efforts to create consensual guidelines require a broad discussion across biomes, regions and public and private sectors. Meanwhile, the Standards should be regarded as a tentative academic‐oriented framework with some substantial limitations for practice.

Taxonomic Diversity and Selection of Functional Traits in Novel Ecosystems Developing on Coal-Mine Sedimentation Pools

Coal-mine sedimentation pools are extrazonal habitats in which the anthropogenic changes of all historic, abiotic, and biotic components, followed by conditions of extreme environmental stress, lead to the formation of novel ecosystems. Our study aims to (i) classify the vegetation on the basis of floristic and ecological criteria, (ii) detect the main environmental gradients responsible for the diversity of vegetation, and (iii) present the selection of species’ functional traits along environmental gradients. A cluster analysis of the floristic data revealed 14 distinct combinations of species. Short- and long-lived ruderals, meadow, xerothermic, and psammophilous species make up the floristic composition of vegetation. A canonical correspondence analysis on the floristic data and average Ellenberg’s indicator values confirmed moisture, soil reaction, and salinity as the main gradients, while fertility and insolation were secondary gradients shaping the diversity of vegetation. A RLQ with a subsequent cluster analysis revealed four groups of species traits selected along environmental gradients. These differed with reference to morphological (canopy height) and physiological traits (specific leaf area, or SLA), as well as persistence (life span), regeneration (reproduction by seeds or vegetative reproduction), and dispersal functional traits. This knowledge can be crucial when planning the restoration of these sites by using spontaneous succession and learning how the various environmental resources can be used to restore or provide new ecosystem services.