Objective: The present study was conducted to developed the Symptom Assessment Scale for Children with Cancer (SAS-CC) and assessed its reliability and validity for Turkish children. Methods: This research was conducted among in 497 children with cancer who were between 7 and 18 years old. The data were collected with a demographic form and SAS-CC. Descriptive statistics, reliability and validity analysis were used to analyze the data. Results: The mean age of children with cancer was 12.02 +3.38 years. The scale consists of 16 items and 3 sub-dimensions. Total factor loads were more than 0.30 in factor analyses. The confirmatory factor analysis revealed all fit indexes as higher than 0.91, and the root mean square error of approximation (RMSEA) was less than 0.080. Cronbach’s alpha values of total was 0.96. According to the split-half analysis, α values of the first and second halves were 0.94 and 0.93, respectively. Conclusions: This study demonstrated that SAS-CC is the first study to develop and test and a valid scale to evaluate symptoms in children with cancer. Effective strategies of coping with symptoms in children with cancer are required to improve prognosis, increase survival, and improve the quality of life. Therefore, assessing symptoms and their frequency in children with cancer is an majority initiative of nurses working in the pediatric oncology clinic.