Spiral Phase Contrast Imaging Research Articles

Near‐Infrared Metalens Empowered Dual‐Mode High Resolution and Large FOV Microscope

AbstractThe spiral phase contrast microscope can clearly distinguish the morphological information of the low contrast objects (i.e., biological samples) owing to the isotropic edge‐enhancement effect, while the bright field microscope can image the overall morphology of amplitude objects. However, the imaging resolution, magnification, and field of view of conventional spiral phase contrast microscopes based on 4f filtering configuration are limited by the system's complexity. Here, compact dual‐mode microscopes working at near‐infrared using the engineered metalens are reported, which can be tuned between the spiral phase contrast imaging and bright field imaging by polarization control. The metalens combine the high‐resolution objective lens and polarization‐controlled phase filter into a single‐layer nanofins array. Two infinity‐corrected microscope systems are demonstrated to achieve subwavelength resolution (0.7λ), large magnification (58X), and large field of view (600 × 800 µm). Unstained onion epidermal is imaged by the microscope to show the dual‐mode imaging ability for the biological sample. Finally, a singlet dual‐mode microscope system is demonstrated to show the edge‐detection application for industrial standards. These results can open new opportunities in applications of biological imaging, industrial machine vision, and semiconductor inspection.

Photo-Aligned Ferroelectric Liquid Crystal Fork Grating-Mediated Fast Switchable Spiral Phase Contrast Imaging

Extensive research has been devoted to spiral phase contrast imaging because of its notable capacity to enhance the edges of both phase and amplitude objects. We demonstrate a setup using ferroelectric liquid crystal (FLC) fork grating (FG) to enable switchable spiral phase contrast imaging within sub-milli-second responses. This system enables the electrical toggling between images featuring edge enhancement and those without it. The specially designed FLC FG generates a vortex beam while in a diffractive state and transmits a Gaussian beam when in a transmissive state. Using a two-step photo-alignment method, the produced FLC FG exhibits exceptional efficiency at approximately 35% and impressively rapid switching at around 307 μs. By introducing this method, we expand the potential applications of spiral phase contrast imaging, particularly in fields such as bio-sensing and photonics.

Dielectric Metasurface for Synchronously Spiral Phase Contrast and Bright-Field Imaging.

Spiral phase contrast imaging and bright-field imaging are two widely used modes in microscopy, providing distinct morphological information about objects. However, conventional microscopes are always unable to operate with these two modes at the same time and need additional optical elements to switch between them. Here, we present a microscopy setup that incorporates a dielectric metasurface capable of achieving spiral phase contrast imaging and bright-field imaging synchronously. The metasurface not only can focus the light for diffraction-limited imaging but also can perform a two-dimensional spatial differentiation operation by imparting an orbital angular momentum to the incident light field. This allows two spatially separated images to be simultaneously obtained, one containing high-frequency edge information and the other showing the entirety of the object. Combined with the advantages of planar architecture and ultrathin thickness of the metasurface, this approach is expected to provide support in the fields of microscopy, biomedicine, and materials science.

Partially coherent spiral phase contrast imaging with a 3D-like effect

Spiral phase contrast (SPC) imaging provides an effective way to isotropic edge detection for biomedical applications. Here, we present a computational SPC imaging method which combines the partially coherent illumination with the symmetry-breaking spiral phase filtering. Such an operation enables the SPC imaging to generate sharp, high-contrast and pseudo three-dimensional edge images that are almost free of speckle. The superior performance is demonstrated by imaging both amplitude and phase objects, and the speckle suppression is validated by comparing the coherent and partially coherent imaging results. This computational SPC method is expected to bring potential advantages to phase contrast imaging modality.

Edge enhancement of phase objects through complex media by using transmission-matrix-based spiral phase contrast imaging

Edge enhancement of phase objects through complex media by using transmission-matrix-based spiral phase contrast imaging

Edge detection by spiral phase contrast imaging at terahertz frequencies

This paper proposes the spiral phase contrast (SPC) imaging at terahertz (THz) frequencies to achieve the target edge detection. The THz SPC imaging is built based on a classical 4f imaging system, and a spiral phase plate is placed in the Fourier plane to generate the spiral phase. Owing to the unique nature of the spiral phase, edge detection can be achieved. The THz SPC imaging mechanism is analysed by the scalar diffraction theory, and its corresponding analytic expression is derived. A circle target is simulated and the resultant image shows a remarkable edge detection. A proof-of-principle experiment is carried out using our designed THz SPC imaging system and the effectiveness of target edge extraction on the THz images is validated. A circle same as the simulation is imaged firstly, which is consistent with the simulated result. And the letter “Z” with more edges is imaged later. All edges in experiments are extracted perfectly, which are extremely consistent with the THz SPC imaging theory.

Non-local spiral phase contrast imaging with thermal light (Invited)

Non-local spiral phase contrast imaging with thermal light (Invited)

Single-pixel spiral phase contrast imaging.

In this Letter, we present single-pixel spiral phase contrast imaging that enables optical edge detection of both amplitude and phase objects. This technique utilizes single-pixel detection to directly acquire the Fourier spectrum of the edge-enhanced object by scanning spiral phase-encoded plane waves in k-space. Experimentally, we exploit a digital micromirror device to simultaneously generate the plane wave and reference field for illuminating the object and scan the plane wave for spectrum sampling. During the process, four-step phase-shifting is adopted, and synchronized intensity measurements are made with a single-pixel detector. Applying an inverse Fourier transform to the obtained spectrum directly yields the edge information of objects. As a demonstration, digital and real objects are imaged, and results verify that isotropic edge detection can be achieved with our technique for both amplitude and phase objects.

Non-local edge enhanced imaging with incoherent thermal light

Spiral phase contrast imaging is an effective technique for the edge enhancement of a phase object. The spiral phase filter is the core component of the system and it provides sensitivity to the phase and amplitude gradients of the object. General spiral phase contrast imaging depends on the 4f imaging system in a single light beam with the coherent light source of visible or infrared wavelengths. Here, we constructed a non-local edge enhanced imaging system using an incoherent thermal light source. The detected object and the adopted spiral phase filter were non-locally placed in two separated light beams. The edge enhanced ghost image of the phase object can then be achieved through second-order intensity correlation measurements. The classical nature of the spatial degree of freedom of our proposed edge enhanced ghost imaging system was also demonstrated through the measurement of Bell-type inequality.

Gradual edge enhancement in spiral phase contrast imaging with fractional vortex filters.

In the spiral phase contrast imaging, the integer spiral phase plate (SPP) are generally employed to perform the radial Hilbert transform on the object. Here we introduce fractional SPP filters, instead of the integer ones, to investigate the gradual formation of edge enhancement for pure phase objects. Two spatial light modulators are used in our experimental configuration. One is addressed to display the pure phase object of a five-pointed star, while the other serves as a dynamic filter of fractional topological charge Q. Of interest is the observation of the complete reversal of the edge and background brightness by gradually changing the fractional vortices from Q = 0 to 1. The experimental results were well interpreted based on the OAM spectra of fractional SPP, which indicates that the filtered output image can be considered as a coherent superposition of all possible images that are individually resulted from the integer OAM filtering. Besides, we show that the spiral phase contrast effect can still be observed in real time for a rotating three-leaf clover. Our results may find potential applications in the optical microscopic imaging.

In-vivo distortion of through-plane flow by spiral phase-contrast imaging

To explain previously unrecognised consequences of two sources of phase curvature over the vessel cross-section in spiral imaging i.e. off-resonance and the velocity-encoded phase-shift.

Real-time color-flow MRI at 3 T using variable-density spiral phase contrast

The purpose of this study was to demonstrate and evaluate the performance of real-time color-flow MRI at 3 T using variable-density spiral (VDS) phase contrast. Spiral phase contrast imaging was implemented within a flexible real-time interactive MRI system that provided continuous image reconstruction and an intuitive user interface. The pulse sequence consisted of a spectral-spatial excitation, bipolar gradient, spiral readout and gradient spoiler. VDSs were utilized to increase spatial and/or temporal resolution relative to uniform-density spirals (UDSs). Parameter choices were guided by specific applications. Sliding window reconstruction was used to achieve a maximum display rate of 40 frames/s. No breath-holding or gating was used. Our results demonstrated that real-time color-flow movies using UDS and VDS provided adequate visualization of intracardiac flow, carotid flow and proximal coronary flow in healthy volunteers. Average aortic outflow velocity measured at the aortic valve plane using VDS was 29.4% higher than that using UDS. Peak velocity measured in the common carotid artery using VDS was 9.8% higher than that using UDS.

Spiral phase contrast imaging in microscopy

We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(i phi) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.