In 1888, Cajal discovered the existence of thin protrusions emerging from the surface of certain neurons. He proposed that these protrusions, which he called dendritic spines, could correspond to points of contact between neurons and beautifully illustrated them with elegant drawings. However, it was not until 1959, with the introduction of electron microscopy, that Gray made the definitive observation which confirmed that dendritic spines are indeed postsynaptic structures that establish synaptic contacts with axon terminals. Since then, spines have received much attention, as they are the major targets of excitatory synapses in the brain. Their density and morphology appear to be indicative of the cellular processes involved in neural plasticity which correlate with cognitive functions such as learning and memory, and are symptomatic in several neuropathologies such as mental retardation and neurodegenerative diseases. Thus, they are believed to be functional integrative units, with a morphology that is closely related to their function, that play an integral role in the activity of spiny cells, yet their exact function still remains unclear. This research topic “Dendritic spines: from shape to function” contains 20 articles that aim to capture the current state of this research field, bringing together some of the latest relevant findings regarding dendritic spine structure and function. It combines 11 reviews, 3 minireviews, 2 original research articles, 3 method articles, and 1 perspective article. Out of the 79 authors that participated in this volume, we would like to dedicate this research topic to Dr. Dominique Muller, a magnificent scientist that contributed to this research topic and regrettably passed away on April 29th 2015 in an accident (Luscher et al., 2015). He was strongly involved in the study of the molecular mechanisms of synaptic network remodeling as well as in synaptogenesis research, providing insight into how synaptic plasticity contributes to brain repair. Indeed, in the present research topic, he and his co-authors review some of these recent advances and discuss the hypothesis that alterations of structural plasticity could represent a common mechanism contributing to the cognitive and functional defects observed in diseases such as intellectual disability, autism spectrum disorders and schizophrenia (Bernardinelli et al., 2014).