Spinach Leaf Extracts Research Articles

Phytoecdysteroids: Quantification in Selected Plant Species and Evaluation of Some Effects on Gastric Smooth Muscles.

Phytoecdysteroids (PEs) are naturally occurring steroid compounds, that have recently gained significant attention, due to their diverse biological activities and high therapeutic potential. The aim of the present study was to quantify some PEs including 20-hydroxyecdysterone (20-HE), ponasterone A (PA), and turkesterone (TU) in selected plant foods and Rhaponticum carthamoides extract. Furthermore, the effects of 20-HE, TU, and R. carthamoides extract, were investigated with in vitro methods using isolated smooth muscle tissues. The levels of 20-HE in the analyzed samples exhibited significant differences, with kaniwa seed extract containing the highest amount, followed by spinach leaf extract, quinoa seed extract, and asparagus stem extract. The in vitro analyses suggested that R. carthamoides extract exhibits dose-dependent cytotoxic effects on smooth muscle cells, with low doses promoting contraction and higher doses inducing relaxation. Additionally, the extract demonstrated a significant inhibitory effect on ACh-induced contractions, while 20-HE enhanced the contractile response. The current findings highlighted phytoecdysteroids' potential for modifying gastrointestinal motility.

Optimizing the green synthesis of antibacterial TiO2 - anatase phase nanoparticles derived from spinach leaf extract

Titanium dioxide nanoparticles, renowned for their abundance, non-toxicity, and stability, have emerged as indispensable components in various fields such as air purification, healthcare, and industrial processes. Their applications as photocatalysts and antibacterial agents are particularly prominent. The synthesis methods significantly influence the properties and subsequent applications of these nanoparticles. While several techniques exist, the biological approach using plant extracts offers advantages such as simplicity, biocompatibility, and cost-effectiveness. This study focused on the green synthesis of titanium dioxide nanoparticles utilizing spinach leaf extract. Within the scope of this investigation, the green synthesis of titanium dioxide nanoparticles through spinach leaf extract were synthesized and optimized, followed by a comprehensive examination of their morphological, structural, and chemical attributes with UV-visible spectroscopy, FTIR spectroscopy, XRD, FESEM, and EDX. The minimum inhibitory concentration (MIC) against E. coli and S. aureus was determined to evaluate their antibacterial potential. Optimal synthesis conditions were identified at 50 °C, using a 1/30 concentration and 20 ml of spinach leaf extract. Spherical anatase nanoparticles, ranging from 10 to 40 nm, were produced under these conditions. The change in the color of the extract, absorption at 247 nm, change and increase of the peak at 800 − 400 wavelengths, and the maximum intensity of X-ray diffraction at the angle of 25.367 with the crystal plane 101 were indications of the synthesis of these nanoparticles. Notably, the synthesized nanoparticles exhibited antibacterial activity with MIC values of 0.5 mg/ml against E. coli and 2 mg/ml against S. aureus. This research presents a novel, eco-friendly approach to synthesizing titanium dioxide nanoparticles with promising antibacterial properties.

Effect of spinach (Spinacia oleracea) leaf extract on the aluminum (Al) as a green corrosion inhibitor in HCl medium

Green corrosion inhibitors have recently attracted much attention due to their non-toxic properties, especially plant extracts. This study used spinach leaf extract (Spinacia oleracea) as a corrosion inhibitor on aluminum in an HCl medium. Spinach leaf extract (Spinacia oleracea) was obtained by maceration method, using ethanol as the solvent, which was then distilled to purify the extract. The corrosion inhibition properties were studied by the gravimetric method. The results showed that spinach leaf extract (Spinacia oleracea) can efficiently decrease the corrosion rate of aluminum in an HCl medium, achieving a maximum efficiency of 96.7742 %. Spinach leaf extract (Spinacia oleracea) formed a protective layer on the aluminum surface via adsorption and chemisorption, thus increasing surface coverage and blocking mass transfers. As the inhibitor concentration increases, the corrosion rate decreases, and the corrosion inhibition efficiency increases. This study shows that spinach leaf extract (Spinacia oleracea) is a viable organic corrosion inhibitor option with high efficiency.

Green marvel: Harnessing spinach leaves' power for enhanced photodegradation of various effluents with biogenic ZnO nanoparticles

This study investigates the use of ZnO NPs (Zinc oxide nanoparticles) derived from spinach leaf extracts in a sustainable photocatalytic technology to enhance the decomposition of hazardous effluents from refineries and paper mills. The biosynthesized ZnO NPs were characterized through X-ray diffraction (XRD), UV-Vis spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The XRD analysis reveals that ZnO NPs possess a hexagonal wurtzite structure, exhibiting a preferred arrangement of (101) planes. The synthesized ZnO NPs have a spherical shape, as seen in the SEM images. TEM analysis detected the size of the ZnO NPs to be between 35 and 40 nm. The results indicate that ZnO NPs produced via biosynthesis have photocatalytic activity in efficiently decomposing the organic chemicals found in gasoline refining effluent when exposed to light. At a pH of 8, the ZnO NPs exhibited the highest rate of degradation for toluene (84.26 %) and xylene (90.36 %) after being exposed to simulated sunlight for 240 min. In the context of the photocatalytic treatment of paper mill effluents, it has been shown that the subsequent cycles resulted in a reduction in chemical oxygen demand (COD) levels of 81.24 %, 74.14 %, 68.92 %, 60.24 %, and 53.82 %, respectively.

Synthesis of TiO2 nanoparticles using red spinach leaf extract (Amaranthus Tricolor L.) for photocatalytic of methylene blue degradation

ABSTRACT Textile industry waste such as methylene blue can pose a serious threat to health and ecosystems. One solution to overcome this problem is to utilize photocatalytic technology using TiO2 semiconductors. This research aims to make green synthetic TiO2 nanoparticles (NPs) using red spinach (Amaranthus Tricolor L.) leaf extract and used for photodegradation of methylene blue. Extraction of red spinach leaves showed that 30 min and a temperature of 95°C showed the highest phenolic content. In this research, several phenolic compounds were also identified in red spinach extract. For TiO2 NPs, the X-ray diffraction results show that TiO2 has a pure anatase phase. Electron microscope results show that TiO2 has the shape of agglomerated spherical clusters. The particle size distribution shows that TiO2 has an average size range of 77–90 nm. The methylene blue photodegradation showed that TiO2 had an efficiency of 90.36% for 180 min, higher than commercial TiO2 Evonic. This result can be attributed to the small particle size and broadband spectra so that photocatalysis is more effective.

PROXIMATE ANALYSIS OF SPINACH (Spinacia oleracea): NUTRITIONAL AND BUSINESS IMPLICATIONS

As the human population is rising, food demands are also surging up that can be met by cultivating wild vegetables, since they are considered low-cost food source among local populations. The current investigation during July 2023 to December 2023 is attempted, to quantitatively evaluate spinach (Spinacia oleracea), that is grown in Tagarapuvalasa, Visakhapatnam, Andhra Pradesh; India; for it is proximate composition levels and anti-microbial activity. Proteins, total carbohydrates and lipids were estimated by following Lowry et.al., [1951], Caroll et.al., [1956] and Bligh and Dyer [1959] respectively. The anti-microbial activity of the leaf extracts was determined by using the protocol reported by Shahriar et al. [2018b] and the zone of inhibition (ZOI) towards the microbes was calculated. From the results of this study, it was found that spinach of the study area is a significant source of macronutrients and micronutrients. Moreover, it also showed the highest anti-bacterial potential as observed in the results of the analysis. Hence, awareness regarding the nutritional and functional properties of these under-utilized leaves may aid towards nutritional and food security in addition to generating novel therapeutic avenues. It may be concluded that, apart from cultivating spinach during the main season, its yield 15 to 30 days early and later main season also may be a more profitable business, not only for a bigger farmer but also to small and marginal farmers. KEYWORDS: Spinach leaf extract, analysis, Nutrients, Anti-bacterial acitivity, Profit.

Effect of addition of red spinach leaf extract (Amaranthus tricolor L.) in feed against the level of color brightness of sword platy fish (Xiphophorus helleri)

Color is important factor ornamental fish, especially sword platy fish, because can affect selling value. One factor influences color brightness is carotenoids. Carotenoids cannot synthesized by fish's body so must be supplied through feed provided. Red spinach is a natural ingredient that contains carotenoids. Aim of research was to determine effect of adding red spinach leaf extract to artificial feed to increase color brightness, growth and survival of Xiphophorus helleri. Research method used a completely randomized design with 4 treatments and 4 repetitions. Concentrations of red spinach leaf extract are 0%, 3%, 6% and 9%. Parameters observed were color brightness, absolute weight, absolute length, survival rate and water quality. Results showed that addition of red spinach leaf extract had a significant effect (P<0.05) on brightness of fish color but had no significant effect (P>0.05) on growth, weight, length and survival rate. Highest increase in color brightness and absolute weight growth was obtained in 9% treatment with an average color brightness score of 6.66, an absolute weight of 0.36 g. Highest absolute length growth occurred treatments C (6%) and D (9%) at 0.41 cm and highest survival rate was obtained treatments A, B and C with a survival percentage of 88%.

PHYSICAL PROPERTIES AND ANTIOXIDANT ACTIVITY OF ESSENCE SHEET MASK WITH CONVENTIONAL AND HYDROPONIC RED SPINACH LEAF EXTRACT

Red spinach is a plant that known as natural antioxidants that contain anthocyanins, flavonoids, betalains, polyphenols and carotenoids and can be developed into sheet mask essence. The antioxidant activity of red spinach affected by the cultivation method. This study aims to determine the physical properties and antioxidant activity of conventional and hydroponic essences with red spinach leaf extract. The making of essence sheet mask starts from making red spinach leaf extract by maceration method using 96% ethanol as solvent for 3x24 hours, then the extract is added to the formulation. Physic evaluations were organoleptic tests including assessing shape, aroma and color by panelists, homogeneity that their data analyzed by descriptively, pH and viscosity analyzed by t-test. Test of antioxidant activity of spinach leaf extract using the DPPH method that analyzed by t-test. Conventional and hydroponic red spinach leaf extracts have a thick green color, thick form and a characteristic spinach odor, while essence sheet masks have a light green color, viscous liquid form and a distinctive fragrance, a safe pH value for facial skin, which is around 5.18 ± 0. 29 to 5.64±0.12, homogeneous and viscosity values ranged from 162.92±21.95 to 187.58±17.54 cps. Based on the results of t-test the pH and viscosity values of all formulations were not significantly different. The antioxidant activity of conventional and hydroponic red spinach leaf extract was in the strong category and not significant difference based on the t-test. The essence sheet mask has good physical properties and the IC50 value of conventional red spinach leaf extract is 52.53 and hydroponic red spinach leaf extract is 73.93.

The effect of adding red spinach leaf extract (Amaranthus tricolor) with different concentrations on the quality of chicken meatballs

Bakso merupakan salah satu produk pangan olahan yang terbuat dari daging dan memiliki komposisi protein, mineral, serta vitamin yang cukup tinggi. Bayam merah (Amaranthus Tricolor) merupakan salah satu sayuran yang kurang populer di kalangan masyarakat. Bayam merah mengandung antioksidan yang tinggi. Penelitian ini bertujuan untuk mengetahui jumlah serta pengaruh penambahan ekstrak daun bayam merah terhadap kualitas bakso ayam yang dihasilkan. Rancangan percobaan yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) dengan faktor konsenstrasi ekstrak bayam merah yang terdiri dari 4 taraf perlakuan yaitu A = 0%, B = 10%, C = 30%, D= 50%. Setiap perlakuan dilakukan 5 kali ulangan sehingga diperoleh 20 satuan percobaan. Hasil Penelitian menunjukkan bahwa penambahan eskstrak daun bayam merah dengan konsentrasi yang berbeda 0%, 10%, 20%, dan 50% berpengaruh nyata terhadap warna, rasa, aroma, dan tekstur bakso ayam yang dihasilkan. Perlakuan terbaik diperoleh yaitu perlakuan B dimana konsentrasi penambahan ekstrak daun bayam sebanyak 10% pada bakso ayam menurut analisis hasil sensori

Utilization of Snapper Spinach Leaf Extract (Amaranthus Hybidrus L) in Peel-Off Gel Mask Preparation for Aging Face Skin Care

The peel-off gel mask is a type of mask that is easy and practical to use. Besides that, it can also hydrate the skin well. Spinach contains chemicals that are good for skin health including vitamin A, vitamin E, vitamin C, and flavonoids. The purpose is to determine the feasibility of peel-off gel masks with snapper spinach leaf extract in terms of laboratory tests (vitamin A, vitamin C, flavonoids, homogeneity, pH, drying time), organoleptic tests (color, aroma, stickiness, and texture), and hedonic test (favourability level). This type of research is an experiment with quantitative analysis. The independent variable is snapper spinach leaves. The dependent variable is the content of vitamin A, vitamin C, flavonoids, homogeneity, pH, and drying time contained in the peel-off gel mask. Data analysis techniques are observation and documentation. The formulations used are X1 (1%), X2 (3%), and X3 (5%). The results showed that in the peel-off gel mask of spinach leaf extract, there was 9568,0000 ppm (9.57%) of vitamin A, 0.088% vitamin C, (+) flavonoids, the X1 and X2 formulations were homogeneous, while the X3 formulation not homogeneous, pH 4.86, and average drying time of 20 minutes. The best formulation in terms of organoleptic and hedonic tests is X2 (3%) quite colorful (55.56%), quite flavourful (66.67%), sticky (66.67%), smooth (100%), likes (66,67%). Thus, the peel-off gel mask with snapper spinach leaf extract is suitable as an aging skin treatment.

PERBANDINGAN AKTIVITAS ANTIOKSIDAN SARI DAUN BAYAM MERAH (Amaranthus tricolor L.) SEGAR DAN REBUS DENGAN METODE DPPH (1,1–diphenyl-2-picylhydrazyl)

One of the highly nutritious vegetables because it contains a lot of protein, vitamin A, vitamin C and mineral salts that are needed by the body and contains anthocyanins which are useful in curing anemia. The purpose of this study was to determine the antioxidant activity and to determine the comparison between fresh and boiled red spinach leaf extract using the DPPH method. The research method used is an experimental method with a post test only control group design research by comparing the differences in the antioxidant activity content of fresh and boiled red spinach leaves. This study used red spinach which was divided into 2 groups, namely the fresh group and the boiled group. Each group of spinach was replicated 5 times using various concentrations (2500 ppm, 5000 ppm, 10000 ppm 15000 ppm, and 20000 ppm) for each replication. A linear regression analysis was performed between the concentration of fresh and boiled red spinach juice. The results showed that there was a very significant difference in antioxidant activity between fresh and boiled red spinach leaves. Fresh and boiled red spinach leaves have weak antioxidant activity due to IC50 150 g/ml. Fresh red spinach leaves have greater antioxidant activity, namely the IC50 value of 237,251 g/ml, while boiled red spinach leaves have an IC50 value of – 279,412 µg/ml.
 Keywords: Red spinach (Amaranthus tricolor L.), Antioxidant, DPPH.

Uji Aktivitas Krim Anti-Aging Ekstrak Etanol Daun Bayam Merah (Amaranthus tricolor L.) pada Kulit Punggung Kelinci New Zealand yang dipapar Sinar UV-A

The strongest cause of premature aging is sunlight (photoaging), especially UV A rays. Anti-aging creams containing antioxidant compounds can be used in the treatment of aging on the skin of the face. Red spinach leaves (Amaranthus tricolor L.) are a natural source of antioxidants that have very strong potential as an anti-aging. Objectives: This study aims to determine the activity of red spinach leaf extract cream as an anti-aging using New Zealand rabbits exposed to UV-A rays. The skin of the rabbit's back is smeared with cream F1, F2, F3, F4, F5 for 30 days. Parameter observation using a skin analyzer is carried out before, after induction, and after cream smearing. Parameters include percent collagen, elasticity, and moisture. The results of the study were analyzed statistically using one way anova. All test creams pass the physical quality test requirements, but the creams that pass the stability test are F1, F2, F4. The primary irritation test F1, F2, F4 is not irritating whereas F3 is very little irritating. Test the ocular irritation of all creams is not irritating. The test results of anti-aging activity show that F2 provides the most effective anti-aging effect because the percent increase in parameters is the greatest.

Green Synthesized Cu2O-Cu(OH)2@Cu Nanocomposites with Fenton-like Catalytic Properties for the Degradation of Cationic and Anionic Dyes

In this work, we introduce an environmental and sustainable approach to grow free standing heterogeneous Cu2O-Cu(OH)2 nanocomposites on a Cu mesh using spinach leaf extract and glycerol. Structural characterizations for samples annealed at 200 °C revealed that there is more Cu(OH)2 than Cu2O on the mesh surface. The photocatalytic activity of the green synthesized catalyst was studied for degradation of a cationic dye methylene blue (MB), an anionic dye methyl orange (MO) and a mixture of both dyes. The effect of changing the dye’s initial pH value on the photodegradation process was explored. After 40 min of irradiation under sunlight, with a maximum intensity of 5 mW/cm2, a basic MB dye (pH-11) showed about 80% color removal with an average kinetic rate of 94.5 m·min−1. In contrast, 93% of the acidified MO dye (pH-2) was degraded with an average kinetic rate of 126.5 m·min−1. Moreover, the versatility of the Cu2O-Cu(OH)2@Cu mesh was evaluated using a remarkable selective separability for a mixture of MB and MO at pH = 2, in the dark and under normal sunlight. Such promising outcomes indicate the potential of our green composites to degrade dyes as both photocatalysts under daylight and as Fenton-like catalysts in darkness.

Effect of spinach-derived glutathione against carbon tetrachloride-induced stress in rats

Background: Increased consumption of natural antioxidants found in vegetables and fruits has been linked to a lower risk of disease caused by oxidative stress.Objective: The current study's focus was to compare the antioxidant properties of synthetic glutathione and glutathione derived from spinach against CCl4-induced stress in female albino rats. Materials and Methods: Cold chloroform extraction was used to prepare the spinach leaf extract. Glutathione was then extracted from the spinach leaf extract using preparative HPLC afterward when the concentration of glutathione was measured in the extract. Biological preventive and therapeutic experiments were conducted, where laboratory rats were divided into 6 groups, the first group G1 (positive control group), the second group G2 (negative control group), the third group G3 preventive experiment 1 (group glutathione extracted from spinach + CCl4), the fourth group G4 therapeutic experiment 1 (Group CCl4+ Glutathione extracted from spinach), the fifth group G5 Preventive experiment 2 (Group of synthetic glutathione + CCl4), the sixth group G6 therapeutic experiment 2 (Group CCl4+ Glutathione extracted from spinach). Results: Treatment of rats with spinach-derived synthetic glutathione, before or after they were given CCl4 subcutaneous, improved the values of parameters near to normal levels in the positive control animals in groups treated with spinach-derived glutathione (G3 and G4) compared to treatment with synthetic glutathione (G5 and G6). Administration of milk with glutathione derived from Iraqi spinach leaves exhibited favorable effects on oxidative stress, greatly enhanced the antioxidant system, and protected rats from liver damage brought on by carbon tetrachloride compared to milk with synthetic glutathione. Keywords: Iraqi spinach, Functional food, Natural antioxidant, Oxidative stress, synthetic glutathione.

Color Changes Of Discolored Human Teeth After Immersion In Spinach Extract In Different Durations

Introduction: Recently, natural dental bleaching agents are being developed to replace chemical dental bleaching agents. Chemical dental bleaching agents containing hydrogen peroxide have the potential to cause toxicity as a side effect. Spinach leaves are natural ingredients that contain oxalic acid which can whiten teeth. Purpose: To investigate the color changes in discolored human teeth after immersion in spinach extract in different durations. Methods: 30 human premolar samples were divided into 3 groups, group A (immersion in 28 hours), group B (immersion in 42 hours), and group C (immersion in 56 hours). All 30 samples were discolored with coffee solution for 12 days and color status (L1, a1, b1) were measured using the chromameter. Then, each 10 samples were immersed in spinach extract according to their treatment group, and the color status (L2, a2, b2) were measured again. Color changes (∆E) were calculated with a formula concerning L, a, b data measured. Results: A significant color change is observed between group A, group B, and group C (p-value= 0.002), and further comparison between three groups showed that group A differed significantly with group C (p-value=0.000), group B also showed significant difference with group C (p-value=0.038). However, there was no significant difference between group A and group B (p-value=0.121). Conclusion: The significant color changes can only be observed after 56 hours of immersion. The spinach leaf extract has the potential to be used as a natural dental bleaching agent.

Investigate the Structural Properties of Silver Nanoparticles Produced by Bio Production of Green Spinicia Oleracea Leaf Extract and their Influence on Antibacterial Activity

This study used Green Spinach Leaf Extract for the green synthesis (monocolors) of silver nanoparticles (AgNPs). This search focuses on the optimization of synthesis by evaluating the impact on the each of the size distribution and activity of antibacterial against bacteria (Escherichia coli) of the green spinach leaf extract volume percent. The characterization of AgNP's was carried out utilizing UV-Visible spectrophotometers, size examination of particles, transmission electron microscopes, energy spectrometry with dispersion X rays, Fourier infrared spectrometer transform and x-ray scanning methods. The experimental data show that AgNPs have been produced effectively and size of particle is controlled by the amount of green spinach leaf extract. With the size reaching (5 nm, polydispersity indices = 0.063 nm), the smaller volume percentage creates AgNPswith a spherically formed reach of 20%. The synthesized AgNPs by the spinach leaf extract had a good antibacterial activity especially at the concentration of (500μg/ml) in which the inhibition zone reached 35mm against gram negative bacteria Escherichia coli.

Green Synthesis of Iron Nanoparticles from Spinach Leaf and Banana Peel Aqueous Extracts and Evaluation of Antibacterial Potential

Spinacia oleracea (spinach) and Musa acuminata (banana) were chosen for the study, and aqueous extracts of spinach leaf extract (SLE) and banana peel extract (BPE) were prepared for the synthesis of iron nanoparticles (FeNPs), and their antibacterial potential against pathogenic bacteria Bacillus subtilis (MTTC 1133) and Escherichia coli (MTTC 62) was evaluated. In 10 minutes at 60°C, the color of the mixture (FeCl3+SLE) changed from light green to dark blackish-brown, and the color of the mix (FeCl3+BPE) changed from transparent yellow to dark black, confirming the synthesis of FeNPs from SLE and BPE, respectively. The UV-Vis spectra of spinach- and banana-derived FeNPs revealed two peaks ranging from 240 to 430 nm and multiple peaks at 240, 270, and 395 nm, respectively. FTIR spectroscopy was used to show different functional groups on BPE and SLE, and their role in FeNP synthesis was predicted. TEM micrographs showed that the particles were in nanoscale, ranging in size from 20 to 50 nm for BPE-derived FeNPs and 10 to 70 nm for SLE-derived FeNPs. The FeNP (BPE and SLE) XRD analysis revealed amorphism, with a weak iron characteristic peak, indicating noncrystallinity. The antibacterial potential of BPE- and SLE-FeNPs was investigated, and inhibition zones (mm) against B. subtilis ( 22.70 ± 0.4 ) and E. coli ( 20.45 ± 1.66 ) were observed, as well as SLE-FeNPs against B. subtilis ( 23.56 ± 1.00 ) and E. coli ( 20.33 ± 0.58 ). There were no significant differences in antibacterial activities between BPE-FeNPs and SLE-FeNPs. Positive controls were tetracycline and gentamicin, both standard antibiotics, at 5 μg/disk. SLE- and BPE-derived green FeNPs were also analysed in vivo of D. melanogaster life history traits, i.e., fecundity, hatchability, viability, and duration of development for toxicity evaluation. SLE- and BPE-derived green FeNPs at a concentration of 10 mg/L were fed flies compared to normal diet-fed flies (control sample), and no significant differences were observed between them. The findings suggest that FeNPs have a high antibacterial potential and could be used as antibacterial agents against pathogenic bacteria while being nontoxic in nature.

STUDI LITERATUR PENGARUH PEMBERIAN EKSTRAK DAUN BAYAM MERAH (Amaranthus tricolor L.) TERHADAP KADAR SGOT DAN SGPT PADA TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK

Background : The prevalence of hyperlipidemia in Indonesia is increasing from year to year, in 2008 it was recorded at 35.1%. Then in 2013 it increased to 35.9%(WHO, 2013). Increased hyperlipidemia triggers Non-Alcoholic Fatty Liver Disease (NAFLD)(Ghanaei et al., 2019). NAFLD conditions cause liver cell damage, causing increased levels of liver enzymes such as SGOT and SGPT (Prahastuti et al., 2020). According to Pradana et al. (2017) it is said that red spinach leaf extract has antioxidant activity that is able to inhibit the incision of the oxidation chain reaction because it contains flavonoid compounds, quercetin, tannins, and saponins which have the potential as a preventive therapy for fatty liver. Objective: To determine the effect of red spinach (Amaranthus tricolor L.) leaf extract on SGOT and SGPT levels in white rats (Rattus norvegicus) fed a high-fat diet. Research Methods: The research method uses a literature study. The database in the literature study was obtained from Google Scholar, PubMed and NCBI which was taken by Population, Intervention, Comparation, Output, Study, and Time (PICOST) from 5,166 journals and finally 8 journals were obtained. Results: Literature from 8 journals is known to have 8 that discuss red spinach leaf extract. Conclusion: From the literature obtained, there was an effect of giving red spinach leaf extract (Amaranthus tricolor L.) on the levels of SGOT and SGPT given a high-fat diet. The dose is 200mg/kgBB and 400mg/kgBB with a duration of administration of 7 to 29 days. Keyword : High fat diet, Red Spinach Extract, Hyperlipidemia, NAFLD, SGOT, SGPT, White Rat

Antibacterial and Antifungal Activity of Green-synthesized Silver Nanoparticles Using Spinacia oleracea leaves Extract

In this study, silver nanoparticles (AgNPs) were green synthesized using aqueous leaf extracts of green spinach that have been used as antimicrobial agents. The antimicrobial efficacy was evaluated against bacterial and fungal strains. The morphological structures, optical and surface properties of as-synthesized nanoparticles have been investigated via a Transmission electron microscope, X-ray diffraction, UV-Vis absorption, and Fourier transform infrared, respectively. The colloidal stability was evaluated using dynamic light scattering and zeta-potential measurements. The average size of as-synthesized particles was about 15±5nm. The green synthesized AgNPs showed significant antibacterial activity against different bacterial species via the agar well diffusion assay. The inhibition zones were varied from 27 to 34mm against 6 bacterial species (K. pneumoniae, E.coli, A. baumannii, P. aeruginosa, S. aureus, and S. haemolyticus). In such a case, the minimal inhibition concertation of AgNPs against bacterial isolates was 4μg/ml for all isolates, except E.coli was 8μg/ml. Also, the silver particles showed an antifungal behavior against 4 fungal species (A. niger, P. marneffie, C. glabrata and C. parapsilosis) with an inhibition zone from 19 to 39mm in diameter. Whereas, the MICs for fungal isolates 8μg/ml for all isolates. Treated Wi-38 cells with AgNPs demonstrated various levels of cytotoxicity dependent on the concentration of AgNPs using MTT assay with IC50  447.6. Moreover, the current study presents a facile and cost-effective synthesis of AgNPs via green chemical methods to be used as antimicrobial agents. Further in-vivo assessment is required to evaluate their therapeutic efficacy against MDR microorganisms.

Novel Method for Fire Retardancy of Cotton Fabrics Coated by Spinach Leaf Extract Assisted by Corona Discharge Plasma

The application of material physics in the functional textile field has been widely applied, one of which is plasma technology. In this research, the fire-retardant properties have been developed in the cotton fabric textile material using spinach leaf extract and comparison of fire-retardant resin on the market using plasma technology. The flame-retardant is applied to the cotton fabric using the atmospheric-pressure plasma method with a tip-cylinder electrode. The cotton fabric used is a cotton fabric that has undergone a process of bleaching and mercerization. In this research, two types of methods have been carried out, namely the immersion method on the fire-retardant resin substance with atmospheric pressure plasma pre-treatment and the immersion method on spinach leaf extract with atmospheric pressure plasma treatment. Research shows that there is an increase in the fire-retardant properties of cotton fabrics that have been given treatment. The assessment of fire-retardant properties was tested using the standard method of fire-retardant ASTM D6413-08 vertical method. In this research, atmospheric-pressure plasma treatment with a time variation of 2 minutes and 4 minutes has been carried out. The results showed that the cotton fabric treated with the immersion method on spinach leaf extract and atmospheric pressure plasma pre-treatment had better fire-retardant properties. It has also been found that the length of time the plasma treatment will affect the fire-retardant properties of cotton fabrics.