ObjectiveTo investigate the relationship of sperm concentration, motility, vitality, acrosome integrity and sperm DNA fragmentation (Df).DesignRetrospective study.Materials and Methods105 patients were evaluated to determinate sperm Df (Halosperm), acrosome integrity (Spermac staining). Semen parameters were evaluated according WHO guidelines. Statistical evaluation was performed through ANOVA (p < 0.05). Patients were divided into three groups according to their degree of sperm Df: 1: ≤ 20%, 2: 20.1% -40% and 3: ≥ 40.1%.ResultsTable ISperm DNA fragmentation (DF), acrosome integrity and WHO seminal parameters, average ± standard deviationGroups1, n=592, n=343, n=12ANOVA (P<0.05)DNA fragmentation (%)13.58 ± 4.0229.21 ± 5.9753.03 ± 12.20*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Acrosome integrity (%)21.27 ± 5.7518.97 ± 4.7717.00 ± 4.19*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Volume (ml)3.48 ± 1.703.60 ± 1.822.89 ± 1.34NSMotility (%)52.68 ± 5.1648.21 ± 8.7137.17 ± 9.52*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Concentration (millions/ml)47.96 ± 43.5729.54 ± 39.9216.31 ± 24.63*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Morphology (%)8.03 ± 4.536.41 ± 4.115.75 ± 4.58NSVitality (%)60.64 ± 8.0461.00 ± 9.2448.50 ± 11.07*Significant difference (P<0.05), NS= non significant difference, n=number of patients.* Significant difference (P<0.05), NS= non significant difference, n=number of patients. Open table in a new tab ConclusionSperm concentration, motility, vitality and acrosome integrity negatively correlated with sperm Df. ObjectiveTo investigate the relationship of sperm concentration, motility, vitality, acrosome integrity and sperm DNA fragmentation (Df). To investigate the relationship of sperm concentration, motility, vitality, acrosome integrity and sperm DNA fragmentation (Df). DesignRetrospective study. Retrospective study. Materials and Methods105 patients were evaluated to determinate sperm Df (Halosperm), acrosome integrity (Spermac staining). Semen parameters were evaluated according WHO guidelines. Statistical evaluation was performed through ANOVA (p < 0.05). Patients were divided into three groups according to their degree of sperm Df: 1: ≤ 20%, 2: 20.1% -40% and 3: ≥ 40.1%. 105 patients were evaluated to determinate sperm Df (Halosperm), acrosome integrity (Spermac staining). Semen parameters were evaluated according WHO guidelines. Statistical evaluation was performed through ANOVA (p < 0.05). Patients were divided into three groups according to their degree of sperm Df: 1: ≤ 20%, 2: 20.1% -40% and 3: ≥ 40.1%. ResultsTable ISperm DNA fragmentation (DF), acrosome integrity and WHO seminal parameters, average ± standard deviationGroups1, n=592, n=343, n=12ANOVA (P<0.05)DNA fragmentation (%)13.58 ± 4.0229.21 ± 5.9753.03 ± 12.20*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Acrosome integrity (%)21.27 ± 5.7518.97 ± 4.7717.00 ± 4.19*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Volume (ml)3.48 ± 1.703.60 ± 1.822.89 ± 1.34NSMotility (%)52.68 ± 5.1648.21 ± 8.7137.17 ± 9.52*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Concentration (millions/ml)47.96 ± 43.5729.54 ± 39.9216.31 ± 24.63*Significant difference (P<0.05), NS= non significant difference, n=number of patients.Morphology (%)8.03 ± 4.536.41 ± 4.115.75 ± 4.58NSVitality (%)60.64 ± 8.0461.00 ± 9.2448.50 ± 11.07*Significant difference (P<0.05), NS= non significant difference, n=number of patients.* Significant difference (P<0.05), NS= non significant difference, n=number of patients. Open table in a new tab ConclusionSperm concentration, motility, vitality and acrosome integrity negatively correlated with sperm Df. Sperm concentration, motility, vitality and acrosome integrity negatively correlated with sperm Df.