Sperm Quality Of Male Rats Research Articles

Potential of ant nest extract (Hydnophytum formicarum) for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan-induced diabetes

Medicinal herbs, such as the ant nest plant (Hydnophytum formicarum), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings. For the experiment, 20 male white rats (Rattus norvegicus), aged 3–4 months and weighing 150–200 g, were equally divided into four groups. The ant nest extract was administered orally using oral gavage over 14 days. The testes, epididymis, and sperm were collected for weighing, morphometric measurements, and quality evaluation. Qualitative testing of phytochemical compounds indicated the presence of flavonoids, tannins, steroids, terpenoids, and phenolic compounds in the plants. The results revealed the protective effects of ant nest extract against the adverse fertility effects induced by alloxan and a high-fat diet, as observed in testicular weight (p=0.045), epididymal weight (p=0.041), and sperm quality (p>0.05).

Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10.

Zinc finger MYND-type containing 15 (ZMYND15) has been documented to play important roles in spermatogenesis, and mutants contribute to recessive azoospermia, severe oligozoospermia, non-obstructive azoospermia, teratozoospermia, even male infertility. ZMYND10 is involved in sperm motility. Whether environmental pollutants impair male fertility via regulating the expression of ZMYND15 and ZMYND10 has not been studied. Arsenic exposure results in poor sperm quality and male infertility. In order to investigate whether arsenic-induced male reproductive toxicity is related to the expression of ZMYND15, ZMYND10 and their target genes, we established a male rat model of sodium arsenite exposure-induced reproductive injury, measured sperm quality, serum hormone levels, mRNA and protein expressions of intratesticular ZMYND15 and ZMYND10 as well as their target genes. The results showed that, in addition to the increased mRNA expression of Tnp1, sodium arsenite exposure reduced sperm quality, serum hormone levels, and mRNA and protein expression of intratesticular ZMYND15 and ZMYND10 and their target genes in male rats compared with the control group (p < .05). Therefore, our study first showed that the environmental pollutant arsenic impairs sperm quality in male rats by reducing the expression of ZMYND10 and ZMYND15 and their regulatory genes, which provides a possible diagnostic marker for environmental pollutants-induced male infertility.

Effect of dressing with different antiseptic agents on reproductive parameters in rats undergoing abdominal incision.

Antiseptic agents used in the postoperative period affect the functions of many tissues in the body, including the testicles. In this study, the effect of dressings administered with different antiseptic agents on testicular functions in rats that underwent abdominal incisions was investigated. A total of 48 Sprague-Dawley rats were used in the study. Each of the rats in the study group underwent a 4 cm-long skin and muscle operation. The incision was then stitched immediately. Antiseptics, hemp seed oil, hemp leaf oil, and cannabidiol oil were then administered to the rats for 10 days to provide antisepsis. The rats were sacrificed 24 hours after the last administration, and testicular tissues were removed. Testicular tissues were used for histopathological examination and biochemical analysis, while epididymal tissue was used for sperm analysis. According to the results, the MDA level in the antiseptic-administered group was higher than in the other experimental groups (p<0.05). Levels of SOD, CAT activities, and GSH content were found to be lower in the antiseptic group than in the hemp seed oil, hemp leaf oil, and cannabidiol oil groups (p<0.05). In testicular histology, the SEED group had the highest Johnsen score, and the antiseptic group had the lowest score (p<0.05). While JAK, P-JAK2, STAT3, PSTAT3, and NF-κB were generally higher in the antiseptic group compared to the other groups, they were lower in the SEED group. Additionally, sperm total motility rate and epididymal sperm density were highest in the SEED group (p<0.05). As a result, it was determined that cannabidiol seed oil had a good effect on testicular histology and sperm quality in male rats during the wound healing process.

Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption

Bee Pollen is a food that has a complete nutritional content including as a source of steroid hormones, helping to improve concentration and brain performance. This study aims to determine the effect of bee pollen on the sperm quality of male rats. Administering 1 ml of water as control (-), 1 ml/kg of Eurycoma longifolia capsule as control (+), 1 ml/kg of nano bee pollen, and bee pollen with concentrations of 0.33 ml/kg, 0.66 ml/kg, 1 ml/kg, and 1.33 ml/kg 34 times in Sprague Dawley male white rats. The mice were then operated on and examined for testicular weight, epididymal weight, sperm pH, sperm motility, sperm concentration, and sperm abnormalities. The results showed that bee pollen and nano bee pollen did not significantly affect the quality of rat spermatozoa. In examining the testicular weight of rats, 0.33 ml/kg of bee pollen had an average testicular weight greater than the control group. For sperm weight and pH of the rat epididymis, the administration of bee pollen showed the average weight and pH were lower than the control. In the examination of sperm motility, 0.33 ml of bee pollen had a higher percentage than the control, while 1 ml of nano bee pollen had the lowest percentage of motility. The results of the concentration and abnormalities of rat spermatozoa from a dose of 1 ml/kg of bee pollen had an average sperm count higher than the control which was 2,129.7 million/ml and the lowest percentage of sperm abnormalities was 8.73%.&#x0D;

Reproductive outcomes of neonatal exposure to betamethasone in male and female rats.

Betamethasone (BM) is the drug of choice for antenatal corticosteroid therapy for women at risk of preterm delivery because it induces fetal lung maturation and enhances survival after birth. However, our group reported evidence of fetal programming and impaired reproductive development and function in rats exposed during the critical window of genital system development. Therefore, we aimed to investigate the effects of BM on the sexual development of rats in the period that corresponds to antenatal corticosteroid therapy in humans. Male and female rats were exposed subcutaneously to BM at 0.1μg/g of pups' body weight or to a NaCl 0.9% solution (control) on postnatal days 1-3. It was observed that neonatal exposure to BM decreased body weight and weight gain in male and female rats during treatment. The estrous cycle was deregulated and LH level was decreased in female rats. In male rats, the sperm concentration in the caput-corpus of the epididymis was decreased, whereas the sperm transit time and sperm concentration in the cauda of the epididymis were increased. Our results demonstrated that neonatal exposure to BM impaired body growth of male and female rats, deregulated the estrous cycle of female rats, and altered sperm quality of male rats. Therefore, BM exposure from postnatal days 1 to 3 corroborated results previously observed after prenatal exposure to this drug. Despite the recognized importance of human antenatal corticosteroid therapy, the findings of this study should encourage further studies in order to minimize possible adverse postnatal effects.

Triphenyltin disrupts the testicular microenvironment and reduces sperm quality in adult male rats.

Triphenyltin (TPT) is organotin that is widely used as an anti-fouling agent and has been determined to have male reproductive toxicity. The objective of this study was to investigate the effects of TPT on the testicular microenvironment and sperm quality in male rats. Adult male Sprague Dawley rats were daily gavaged with TPT (0, 0.5, 1, and 2mg/kg body weight) for 28 days. The results showed that TPT dose-dependently decreased sperm count and sperm motility, interfered with sperm histone-protamine replacement process, and significantly increased sperm deformity rate, but did not affect sperm DNA integrity. TPT at 2mg/kg significantly decreased the gene and protein expressions of testis PCNA and Ki67, and dose-dependently decreased the number of PCNA-positive cells and Ki67-positive cells. TPT at 1mg/kg and/or 2mg/kg down-regulated the expression of StAR, SF1, P450scc, FSHR, WT1, DDX4 and PLZF, and up-regulated SOX9 expression. Simultaneously, TPT reduced serum testosterone levels at each dose and dose-dependently decreased the expression of Leydig cells regulators (INSL3, IGF1, inhibin B) and Sertoli cells regulators (GDNF, FGF2, CXCL12, ETV5), altered testicular microenvironment. Further, in vitro, we treated TM3 (Leydig cells), TM4 (Sertoli cells) and GC-1 (spermatogonia) cells with 1-100nM TPT for 24h. 100nM TPT significantly down-regulated the expression of the above indicators in TM3 and TM4 cells but did not directly affect the cell proliferation ability of GC-1. However, after co-culturing TPT-treated TM3 or TM4 cells with GC-1cells, it was found that TPT-treated TM3 or TM4 cells dose-dependently reduced the gene and protein expression levels of PCNA and Ki67 and increased cytotoxicity in GC-1cells. In conclusion, TPT impairs the proliferative ability of spermatogonia by disrupting the microenvironment of Leydig cells and Sertoli cells, which in turn leads to low sperm quality in adult male rats.

Ameliorative effect of methanolic extract of Tribulus terrestris L. on nicotine and lead-induced degeneration of sperm quality in male rats

Ethnopharmacological relevanceThe use of herbal and medicinal plants to treat male infertility is well known in history. Tribulus terrestris L. (TT) belongs to the Zygophyllaceae family and it is used in folk medicine to vitalize and also improve both physical performance and sexual function in men in addition to the protective effect of the gross saponins of TT against ischemic stroke and its clinical anti-inflammatory property.Aim of the study: This study aimed to investigate the effects of methanol extract of T. terrestris on nicotine hydrogen tartrate and lead-induced degeneration of sperm quality in male rats and to identify the volatile bioactive non-polar compounds thought to be responsible for its activity using gas chromatography-mass spectrometry (GC-MS). Materials and methodsThe effect of T. terrestris on nicotine hydrogen tartrate and lead-induced infertility was evaluated in male rats. Fifty-four mature male albino rats weighing 220–250 g body weight were used. The rats were randomly divided into 9 equal groups (n = 6). Infertility was induced by administering nicotine hydrogen tartrate (0.50 mg/kg) through peritoneal injection (i.p.) or lead acetate (1.5 g/L) orally with drinking water for sixty days. Two doses (50 and 100 mg/kg body weight of the animal) of T. terrestris were also used. At the end of the experimental period, the rats were anesthetized and sacrificed. Blood samples were collected. Hormonal analyses were carried out on the serum. The testicle, epididymis, and accessory sex organs (seminal vesical and prostates) were removed for histopathological analysis. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanol extract was also carried out to identify major volatile compounds in T. terrestris methanol extract. ResultsNicotine and lead toxicity caused a significant (p < 0.05) decrease in the number of sperm, motility, and an increase in the sperm abnormalities such as the reduction in weight and size of sexual organs (testis, epididymis, and accessory sex glands), reduction of diameter and length of seminiferous tubules. The administration of T. terrestris methanol extract, however, improved the semen quantity and quality, sexual organ weights, and fertility of male rats and, thus, ameliorated the adverse effects of nicotine and lead. Ten major compounds were found from the GC-MS analysis of the extract of T. terrestris methanol extract. ConclusionFindings showed that T. terrestris plant methanolic extracts ameliorated nicotine hydrogen tartrate and lead-induced degeneration of sperm quality in male rats. The GC-MS analysis of the T. terrestris plant methanolic extracts revealed the presence of several important bioactive compounds which were thought to be responsible for the ameliorative effect. Further isolation and evaluation of the individual components would provide relevant lead to finding new drugs.

The effect of resistance training on serum levels of sex hormones and sperm quality in male rats under X-ray radiation.

The present study aims to investigate the effects of resistance training on sex hormones and sperm parameters in male rats under X-ray. In this experimental study, 24 Sprague Dawley rats (200-250g) were randomly assigned into four groups: healthy control, irradiated control, healthy training and irradiated training. Irradiation was induced at a dose of 4Gy on the whole body. The resistance training protocol was performed for 10weeks. Finally, blood serum was used to assess FSH, LH and testosterone and sperm quality. Data were analyzed using ANOVA and Tukey's post hoc test. The results showed that radiation significantly reduced serum levels of LH (p=0.42), FSH (p=0.001) and testosterone (p=0.28) between radiation control and healthy control groups. Also, no significant difference was observed between serum levels of LH (p=0.135) and testosterone (p=0.419) in radiation resistance training and the healthy control groups. In addition, significant differences were observed between radiation resistance training and radiation control groups in sperm parameters such as sperm count (p=0.02) and progressively motile sperm (p=0.031). It seems that short-term resistance training can improve sperm parameters, including sperm count and sperm motility through increasing serum levels testosterone and LH in male rat under X-ray.

Dracaena arborea (Dracaenaceae) Increases Sexual Hormones and Sperm Parameters, Lowers Oxidative Stress, and Ameliorates Testicular Architecture in Rats with 3 Weeks of Experimental Varicocele.

Varicocele is a disease characterized by an abnormal dilation of the pampiniform plexus that drains the testis. The main objective of this work was to evaluate the curative effects of aqueous and ethanolic extracts of Dracaena arborea on some reproductive and antioxidant markers in rats with experimental varicocele. Following varicocele induction, rats (5 per group) were randomly partitioned into untreated varicocele, vitamin E-treated (150 mg/kg), aqueous extract-treated (500 mg/kg), and ethanolic extract-treated (100 mg/kg) animals. Two other groups served as normal and sham-operated. After 2 or 4 weeks of treatments, body and sex organ weights, spermatozoa characteristics, antioxidant status, NO level, sex hormones, and testis histology were measured. Animals with 3 weeks of varicocele showed a significant (p < 0.05–0.001) decrease in body and sex organ weights, total proteins, sperm characteristics, testosterone concentration, SOD, catalase, and total peroxidase activities. An increase in the plasmatic FSH, LH, and testicular MDA and NO concentrations was also recorded. Moreover, marked disorganization of the testicular architecture was observed. Treatment with D. arborea significantly reversed these impairments due to varicocele. For instance, after 4 weeks, treatment with aqueous extract of D. arborea significantly (p < 0.05–0.001) increased testes and epididymis weights, sperm viability (89.12 ± 1.09 vs 68.22 ± 1.42), sperm density (148.50 ± 2.59 vs 110.25 ± 2.51), and sperm motility (68.16 ± 2.39 vs 55.88 ± 3.20) in the left side, compared with varicocele-untreated rats. The extract also significantly (p < 0.05–0.001) decreased malondialdehyde level (2.19 ± 0.04 vs 3.50 ± 0.13) but elevated catalase (0.97 ± 0.03 vs 0.55 ± 0.03), SOD (0.5 ± 0.03 vs 0.15 ± 0.03), and peroxidase (65.80 ± 2.9 vs 40.95 ± 2.44) activities. Present results showed that D. arborea extracts possess antioxidant effects and improve sperm quality in male rats with an existing varicocele.

Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

PurposeThis paper aims to design to evaluate the protective effect of vitamin E to ameliorate the disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine (V) and convicine (C) of Vicia faba.Design/methodology/approachForty male albino rats were divided into five equal groups; control, paraffin oil, V (400 mg/kg) C (150 mg/kg)-treated group, vitamin E (100 mg/kg) + VC-treated group, and vitamin E (200 mg/kg) + VC-treated groups which injected intraperioneally (IP) with 0.5-ml saline, 0.5-ml paraffin oil,V (400 mg/kg) and C (150 mg/kg) of Vicia faba, vitamin E (100 mg/kg) + VC-treated groups, and Vitamin E(200 mg/kg) + VC-treated groups, respectively. Blood and testicular tissue were obtained after one month of the study. The male genital organs were calculated. Testosterone (Ts), luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone sulfate (DHEA-SO4), sex hormone binding globulin (SHBG),?-glutamyl transpeptidase (?-GT), glucose-6-phosphate dehydrogenase (G6PD), 3ß-hydroxysteroid dehydrogenase (3ßHSD), lactate dehydrogenase (LDH), spermatozoa concentration, percent of mortality and abnormal sperms were evaluated.FindingsThe VC-treated group showed significant decrease (p < 0.01) in Ts, DHEA-SO4, G6PD, spermatozoa number and mortality percent, as well as, the male genital organs (testes, epidydemis, seminal vesicle, prostate and vasa deferentia) while significant increase (p < 0.01) was found in LH, FSH, SHBG, LDH, ?-GT, sperms monoclonal Ki-67, and abnormal spermatocytes levels compared with control group. Vitamin E co-injection with VC-treated group returned all these parameters to the normal values. The higher dose of vitamin E (200 mg/kg) was more effect than the lower dose (100 mg/kg).Originality/valueVicia faba contains V and C glycosides. The V and C glycosides in Vicia faba are hydrolyzed by intestinal microflora to aglycones divicine and isouramil, respectively. Divicine and isouramil are highly reactive compounds generating free radicals where divicine and isouramil are the main factors of favism. The V and C glycosides induced disturbances in testosterone pathway and sperm quality of male rats and vitamin E ameliorates these disturbances.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

Effect of Low-Intensity Endurance Training and High-Intensity Interval Training on Sperm Quality in Male Rats with Fatty Liver.

BackgroundWe aimed to investigate the effect of low-intensity endurance training (LIET) and high-intensity inter- val training (HIIT) on sperm parameters, chromatin status, and oxidative stress in a rat model of non-alcoholic fatty liver disease (NAFLD).Materials and MethodsFor this experimental study, we divided 40 male Wistar rats into four groups (control, sham, HIIT and LIET) according to diet treatment and exercise training protocol. Liver triglycerides, sperm parameters, sperm lipid peroxidation (BODIPY C11 probe) and chromatin status [chromomycin A3 (CMA3)], and acridine orange [AO] staining) were assessed in these groups at the end of the study.ResultsThe mean liver triglyceride values significantly improved in both the LIET and HIIT groups compared to the control and sham groups. The mean of testicular volume, sperm concentration, motility, intensity of sperm lipid peroxidation and DNA damage were similar within groups. While, the mean percentage of sperm lipid peroxidation and protamine deficiency were significantly higher in the LIET and HIIT groups compared to the control group.ConclusionBoth LIET and HIIT in the rat NAFLD model had no adverse effects on testicular morphometric param- eters, sperm concentration, motility, and DNA integrity. However, the mean sperm lipid peroxidation and protamine deficiency were significantly higher in both exercise groups. Our study suggests that exercise or antioxidant supple- mentation could minimise the adverse effects of oxidant by-products of exercise.

Evaluation of sperm quality in male rats treated with Sauropus androgynus (L.) merr. leaf fractions

Sauropus androgynus (L.) Merr. or katuk is one of the medicinal plants broadly used in Indonesia as it has active compounds that can, among others, stimulate reproductive hormones. This study was aimed to determine which active fraction of S. androgynus leaves that have the potential to improve the sperm quality of male rats based on three parameters, namely sperm count, viability, and motility. It employed fractionation using the liquid-liquid technique with n -hexane, ethyl acetate, and water solvents to obtain the fractions. Twenty-four mature Sprague-Dawley male rats were divided into four equal groups: the normal group (untreated group) received 0.5% Na-CMC suspense, and the three fraction groups were given the n -hexane, ethyl acetate, and water fraction p.o., respectively, at the dose of 11.85 mg.Kg -1 BW daily for seven days. Sperm count, viability, and motility were measured on Day 8 (after treatment) from the sperm samples collected at the cauda epididymis of the sacrificed test rats. The results showed that compared with the normal group, the n -hexane and ethyl acetate fractions significantly increased the three parameters (p&lt;0.05). Therefore, the S. androgynus leaf fractions have the potential as a natural material that can increase the fertility of male rats.

Sperm Quality Reduction of Rats (Rattus novergivus) Injected by High Dosage of White Vitamin C in Long Term

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase.&#x0D; Keywords: white vitamin C, rat, sperm quality

Safety evaluation of supratherapeutic dose of Maytenus ilicifolia Mart. ex Reissek extracts on fertility and neurobehavioral status of male and pregnant rats

Maytenus ilicifolia Mart. ex Reissek is a plant commonly used in folklore medicine in the management of gastric diseases in South America. This study explores the effects of a supratherapeutic dose of aqueous and ethanol extracts of M. ilicifolia (1360 mg/kg) on fertility and neurobehavioral status in male and pregnant rats. A battery of sensory-motor developmental endpoints was carried out to assess impairments on pups of dams orally treated with the aqueous extract of M. ilicifolia during the organogenesis period of pregnancy (GD 9 through GD 14). The neuromotor maturation reflexes and physical developments of the offspring were not significantly different between the groups (p < 0.05). Also, the hippocampal morphology revealed no indices of cell loss in the CA1, CA2, CA3 and CA4 areas. As second protocol, some fertility aspects were investigated in young post pubertal male Wistar rats treated with the ethanol extract for 30 days. The semen quality and testicular tissue morphology of male rats treated with the ethanol extract of M. ilicifolia remained unaffected upon treatment. Thus, the results indicate that the high-dose of M. ilicifolia extracts have no neurotoxic potential on offspring and seem not to affect the sperm quality of male rats.

Aluminium-Induced Oxidative Stress, Apoptosis and Alterations in Testicular Tissue and Sperm Quality in Wistar Rats: Ameliorative Effects of Curcumin.

Reproductive toxicity is a major challenge associated with aluminum (Al) exposure. No studies have evaluated the possible effects of curcumin (CUR) on Al-induced reproductive dysfunction. Therefore, this study investigated the effects of CUR treatment on Al-induced reproductive damage. In this experimental study, 40 male Wistar rats were allocated to the five groups (n=8) based on the treatment they received: no treatment (control), solvent [dimethyl sulfoxide (DMSO) or distilled water], CUR 10 mg/kg body weight (BW), Al chloride 10 mg/kg BW, and CUR+Al chloride (10 mg/kg BW/each alone). Treatments were performed by intraperitoneal (IP) injections for 28 days. The left testis was assessed for histopathological analysis as well as the incidence of germ cell apoptosis. One-way analysis of variance (ANOVA) followed by the Tukey's test was used. P<0.05 was considered significant. Significant reductions in body and testis weight; plasma testosterone and luteinizing hormone levels; sperm count, motility, morphology, and viability; germinal epithelium thickness; seminiferous tubules diameter; as well as, superoxide dismutase activity were observed in rats treated with Al. Moreover, Al exposure caused significant increments in the lumen diameter of tubules, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells and malondialdehyde (MDA) levels compared to the control group. However, in rats receiving CUR+Al, CUR significantly reversed the adverse effects of Al on testis and sperm quality. No significant differences in follicle-stimulating hormone (FSH) levels and nuclear diameter of spermatogonia were detected among all groups. It can be concluded that Al causes reproductive dysfunction by creating oxidative damage. CUR, on the other hand, reduces the toxic effects of Al and improves the antioxidant status and sperm quality in male rats.

Effects of Acrylamide on the Reproductive Hormones and Sperm Quality in Male Rats

Effects of Acrylamide on the Reproductive Hormones and Sperm Quality in Male Rats

Comparison between Effect of Sildenafil Citrate and Tramadol on Testis and Sperm Quality of Male Rats

Comparison between Effect of Sildenafil Citrate and Tramadol on Testis and Sperm Quality of Male Rats

Comparison between the Effect of Sildenafil Citrate and Tramadol on Testis and Sperm Quality of Male Rats

Comparison between the Effect of Sildenafil Citrate and Tramadol on Testis and Sperm Quality of Male Rats

Effects of malathion on testicular enzyme activities and sperm quality of male rats

The aim of this study is to investigate the effects of sub chronic exposure to malathion on testicular enzyme activities and sperm quality of male rats. Forty male rats were divided into four groups: three exposure groups and a control group. Malathion was administered orally to male rats at 0, 33.75, 54.00, and 108.00 mg/kg for 60 days to evaluate the toxic alterations in sperm dynamics and testicular enzyme activities including ACP,LDH,SDH and γ-GT. The control rats were administered with an equivalent volume of distilled water in the same manner.After sacrificed, the testes were collected and weighed. The body weight and the testis weight of animals showed a decreasing tendency, and there was a statistical difference between the 54.00, 108.00 mg/kg groups, and the control group (P<0.05). Malathion brought about marked reduction in testicular sperm counts, sperm motility, and significant growth of sperm malformation rate in 108.00 mg/kg group. A significant decrease in the activities of testicular enzyme ACP and γ-GT was observed in malathion exposed rats, while the activities of LDH was significant increased and there were no obvious effects on the activities of SDH. The activities of ACP, γ-GT and LDH showed a statistical difference between the 108.00 mg/kg groups, and the control group. Malathion reduced the sperm counts and sperm motility, increased the malformation rate and reduced the activities of testicular enzymies of male rats.