Work zones on two-lane highways with one lane closed require sharing the open lane with traffic from the closed directions. In such work zones, traffic control resembles operating a two-phase traffic signal and, in rare cases, a three-phase traffic signal. Temporary traffic signals (or flaggers) allow the open lane to be used in an alternating manner. Signal timing (green times and cycle length) of the temporary traffic lights directly affects the delay and queue in the work zones. Delay and queue computations must consider queue build up that often happens in oversaturated conditions. Another influential variable is the average operating speed of the work zone. The operating speed is affected by the work zone speed limit, work intensity, speed control technique, lane and shoulder widths, acceleration capability of vehicles, and work zone length. WorkZoneQ-Pro (WZQ-Pro) is developed with a new signal timing method that considers the above-mentioned factors. The procedure can handle multiple hours of analysis with two- or three-phase signal operations. Test scenarios are real-world work zone examples from three different U.S. states that were used to compute signal time variables and use them to compute queue and delay. These values were also computed using Highway Capacity Manual (HCM) 2016 procedures and are compared. In addition, the computed values were input into Vissim simulation software, and the results were compared. It showed that the WZQ-Pro results are reasonably close to Vissim simulation results, and that further validated that an acceptable agreement existed between the analytical and simulation results.