Abstract The results of airborne measurements and statistical characteristics of mesoscale fluctuations of wind velocity, temperature, and concentrations of gas constituents at different heights of a stably stratified troposphere are presented. The measurements were carried out in September 2022 in the Arctic region of Russia with the aircraft laboratory Tu-134 “Optik.” The obtained spectra and structure functions of the fluctuations are interpreted with the theoretical model of formation of the spectrum of mesoscale wind velocity and temperature fluctuations described in the paper. The presence at high wavenumbers of a steep section in the obtained horizontal wavenumber spectra of the fluctuations of wind velocity and greenhouse gas concentration with a slope close to −3 is discussed. The fluctuation spectra along different slanted tracks of the aircraft crossing the tropospheric layer between altitudes of 1 and 9 km are also obtained and analyzed with the theoretical model.