The aim of the study is to validate and calibrate the Doppler spectral-domain optical coherence tomography (SD-OCT) derived total retinal blood flow (TRBF) and metabolic hyperspectral retinal camera (MHRC) derived oxygen saturation (SO2) of major retinal vessels in human volunteers using a novel and exact provocation technique (RespirAct) that allows the precise control of the end-tidal partial pressure of oxygen (PETO2). Between visit repeatability of the TRBF and retinal blood SO2 also were studied. One eye of 11 young healthy subjects was chosen randomly for the study. Total retinal blood flow and retinal SO2 measurements were obtained under conditions of normoxia, hyperoxia, and hypoxia. The order of hyperoxia and hypoxia was randomized between subjects. The measurements were repeated after a week interval. When the arterial PETO2 was increased from baseline (PETO2 = 100 mm Hg) to 200 and 300 mm Hg, the TRBF significantly reduced (P = 0.02) from 44.60 (±8.9) to 40.28 (±8.9) and 36.23 (±4.6) μL/min. Lowering the arterial PETO2, from baseline to 80, 60, and 50 mm Hg, TRBF significantly increased (P = 0.04) from 43.17 (±12.7) to 45.19 (±5.5), 49.71 (±13.4), and 52.89 (±10.9) μL/min with simultaneous reduction in the arterial blood SO2 content from 99.3% (±5.8) to 95.6% (±5.1), 89.6% (±2.8), and 83.3% (±3.9), respectively (P = 0.00). The coefficient of repeatability (COR) of TRBF, retinal arterial, and venous blood SO2 values are 21.8 μL/min, 18.4%, and 15.2%, respectively. Total retinal blood flow and retinal blood SO2 measurements performed under safe levels of hypoxia and hyperoxia were repeatable in healthy adults over the range of SO2 investigated.