Specific Turbidity Research Articles

Intercomparison of optical scattering turbidity sensors for a wide range of suspended sediment types and concentrations

To monitor the effects of rapid changes in climate and land use on sediment export from erodible environments, it is crucial to accurately quantify highly fluctuating suspended sediment concentrations (SSCs) in contrasted river systems that drain small to mesoscale catchments. To this end, we investigate the turbidity-based quantification of SSCs in the range of 0.05–100 g/L through laboratory experiments performed with 7 different types of turbidity sensors and sediments from 10 watersheds. We find that measurements of scattered light from multiple angles may allow for: (1) an extended monitoring range with SSCs up to 10–100 g/L, where enhanced uncertainty may occur near the transition in the effective operational ranges of the underlying signals (typically somewhere in the range of 1–10 g/L); and/or (2) a slightly reduced sensitivity to sediment properties. The specific turbidity of the investigated sensors is inversely related to particle diameter (D10) for SSCs up to 1–5 g/L. Backscatter and combined-signal sensors also show a dependency on sediment colour (CIE a∗), which becomes particularly prominent at SSCs above 10 g/L. We relate this increase in colour dependency with SSC to the expected effect of cumulative near-infrared light absorption associated with multiple scattering. We discuss covarying physical properties of naturally occurring river sediment that can dampen or enhance measurement sensitivity and result in turbidity-based SSC rating curves that may strongly differ in magnitude and form from curves derived for industrially prepared material that is often used for sensor calibration. Although the differences in SSC per sensor among sediment types are generally less than one order of magnitude, the systematic errors and uncertainties associated with high SSCs are typically greater than one order of magnitude and may disproportionally affect the quantification of sediment loads during large-magnitude flow events.

Water Motor and Water Quality Management Using Wifi and Blynk App

Abstract: As population is bound to expand and water is being used at the utmost level of exploitation, groundwater level is diminishing rapidly, the planet might face water emergency soon. India is an agrarian country, farmers rely upon groundwater, however to their needs and satisfaction the predictability of rainfall and a suitable weather is quite flawed. So they are compelled to dig borewells to a prominent depth and conclusively rely upon it. Larger part of individuals living in metropolitan regions are subjected to borewells as there could be no other option for water. The progressions in innovation like Internet of Things, Cloud computing, Big Data, Internet facility can be utilized to plan a framework to screen these gigantic significant borewells to beat impediments like low quality drinking water, expanded labor prerequisites, irreversible harm caused to inadequately checked public motors. Plan and advancement of checking pH, turbidity in the water and observing motor temperature and momentum in borewells would be of extraordinary use. Additionally borewells are not midway checked prompting sudden breakdown of the motor. Borewells are the indispensable source of water for individuals in a large portion of the towns and practically all urban areas to achieve their essential prerequisites of a day. The framework has two sensors for to be specific turbidity and pH sensor to check water quality and temperature sensor and current sensor for motor monitoring, Ardunio model and , Single microcontroller chip, a wifi chip, MpH sensor, is utilized to send the information of different borewells to a solitary unit. Subsequently by this framework it is feasible to monitor n number of borewells in a municipality somewhat in one mobile application Keywords: M pH sensor, Turbidity sensor, Temperature sensor, Flow sensor, Ardurino model, WI-FI module.

Development of an Irrigation Water Quality Database to Identify Water Resources and Assess Microbiological Risks During the Production of Fresh Fruits and Vegetables

An Irrigation Water Quality Database was developed to help assess the microbiological quality of irrigation water used in fruit and vegetable production in 15 counties in New York (NY) State. Water samples from Tennessee (TN) were also included in the database. Four water quality parameters, quantified generic Escherichia coli, specific conductance, pH, and turbidity, were tested. Ground, reservoir, and running water were sampled over 2 years (2009 and 2010), covering three seasons each year (spring, summer, and fall). TN data are for all three seasons in 2010 only. Overall in NY (254 total samples), ground water had a geometric mean of 1 most probable number (MPN)/100 ml, reservoir water had a geometric mean of 8 MPN/100 ml, and running water had a geometric mean of 52 MPN/100 ml. Overall in TN (63 total samples), ground water had a geometric mean of 1 colony forming unit (CFU)/100 ml, reservoir water had a geometric mean of 5 CFU/100 ml, and running water had a geometric mean of 38 CFU/100 ml. These values are all below the 126 MPN/100 ml United States Environmental Protection Agency's Ambient Water Quality Standards (AWQS) standard for fresh water. The presence of E. coli had very weak but sometimes statistically signficiant correlatation with water specific conductance, pH, and turbidity, depending on the water source but the r-squared effect was not strong enough to make the other measurements a substitute for testing specifically for E. coli in water.

Investigation of the flocculation and sedimentation of TiO2 nanoparticles in different alcoholic environments through turbidity measurements

Turbidimeters are low-cost devices, which are widely used for suspended sediment monitoring (SSM). The specific turbidity is typically relative to 1/d (d is the diameter of particles) regarding the suspensions with mono-sized sphere-shaped particles. As the production of the dispersed suspension of TiO2-NPs is vital for their photocatalysis applications, turbidity provides a test to measure the dispersion of TiO2-NPs. In the present paper, the performance of a self-manufactured turbidimeter device has been investigated using the suspensions of TiO2-NPs in different alcoholic media. The results showed that over time, the intensity of light passing through the upper part of the test tube containing TiO2-NPs suspension increased, suggesting the settlement of TiO2 nanoparticles. In the middle part of the test tube; however, an almost stable trend was observed, which was more evident in the case of isopropanol with higher viscosity. The results also illustrated that there was no relation between the concentration of suspension and the value of transmitted light for concentration below 0.08 g/l; however, for concentrations above that, the intensity of transmitted light decreased with the increase of suspension concentration.

Revisiting the Gage–Bidwell Law of Dilution in Relation to the Effectiveness of Swimming Pool Filtration and the Risk to Swimming Pool Users from Cryptosporidium

The transfer of water from a swimming pool to the treatment location is key in determining the effectiveness of water treatment by filtration in removing turbidity and managing the risk from particulate material, including microbial pathogens, such as Cryptosporidium spp. A key recommendation for pool operators when dealing with an accidental faecal release (the likely main source of high Cryptosporidium oocyst concentrations in pools) is that the pool water should be filtered for at least six turnover cycles prior to use. This paper briefly outlines the theoretical basis of what has become known as the Gage–Bidwell Law of Dilution, which provides a basis for this recommendation, and extends the idea to account for the impact of filter efficiency. The Gage–Bidwell Law reveals that for each pool turnover 63% of the water resident in the pool at the start of the turnover period will have been recirculated. Building on this, we demonstrate that both filter efficiency and water-turnover time are important in determining filtration effectiveness and can be combined through a single parameter we term ‘particle-turnover’. We consider the implications of the Gage–Bidwell Law (as referred to in the original 1926 paper) for the dynamics of the ‘dirt’ content of pool water, whether in terms of a specific particle size range (e.g., Cryptosporidium oocysts) or turbidity.

Effects of the Method of Preparation and Dispersion Media on the Optical Properties and Particle Sizes of Aqueous Dispersions of a Double-Chain Cationic Surfactant.

As inferred from visual observations and turbidity measurements, the average radius of the unilamellar vesicles formed in water from the cationic double-chain surfactant didodecyldimethylammonium bromide (DDAB) varies with the method of preparation, being ∼24 nm after sonication (SS method) and ∼74 nm after extrusion/ultrafiltration (SE method). The radii were larger when the vesicles were produced in 10 mM NaBr, ∼65 nm for the SS method and ∼280 nm for the SE method. The specific turbidity, or turbidity per unit path length divided by the surfactant weight fraction, w, of these vesicular dispersions increased with decreasing w until a constant value was reached at w*, which depends on the preparation method and the dispersion medium. The constant specific turbidities are indicative of single and independent scattering and were used to estimate vesicle radii by solving the specific turbidity equations derived for the Rayleigh-Debye-Gans (RDG) regime. Two turbidity equations were used, one accounting for absorbance errors due to some scattered light reaching the detector and another with no correction. Estimates of the average distances between the vesicles and their corresponding Debye lengths were obtained for evaluating the importance of intervesicle electrostatic interactions, which could lead to dependent scattering at higher weight fractions.

A national‐scale study of spatial variability in the relationship between turbidity and suspended sediment concentration and sediment properties

AbstractTurbidity, an index of light side‐scattering, depends on the mass concentration of suspended sediment (SS) within water. Turbidity of river water is regulated by the presence of suspended particulate matter and is used to identify visual changes in response to SS. We used data from the New Zealand National River Water Quality Network, to calculate “specific turbidity” (K; turbidity normalised to mass concentration of suspended particulates). Specific turbidity is shown here to be an effective metric to assess the effect of suspended material composition and particle size distribution of suspended particulate matter over different landscape characteristics, and the effect on SS‐turbidity relationships. Of the catchment characteristics considered in our study, specific turbidity was most responsive to lithological factors, and relatively insensitive to land use and soil parameters. Decreasing particle size has a positive linear response to K, attributed to the higher proportion of ultra‐fine particulate material that is generated by certain lithologies, underscoring the lithological influence on K. SS‐turbidity relationships, therefore, vary spatially across New Zealand's national record of water clarity, and K is considered a useful index for inter‐catchment comparison of SS than turbidity alone.

Rayleigh and Rayleigh-Debye-Gans light scattering intensities and spetroturbidimetry of dispersions of unilamellar vesicles and multilamellar liposomes

Hypothesis: Since the volume fraction of the surfactant bilayer(s), of thickness db, in a vesicle and liposome is smaller than one, the dependences of the Rayleigh (R) scattering intensity and turbidity on the particle radius a are weaker than those for a homogeneous sphere, which are proportional to a3. The dependences of the Rayleigh-Debye-Gans (RDG) scattering intensity and turbidity on a are also weaker. Work done: The dependences of the effective relative refractive index on a, db, and dw (water layer thickness) were derived. The specific Rayleigh ratio Rθ** and the specific turbidity τ** for single and independent scattering were derived analytically for R and RDG scattering. Spectroturbidimetry data at 25°C for a cationic double-chain surfactant, didodecyldimethylammonium bromide (DDAB) were compared to the turbidity predictions. Findings: For R scattering, Rθ** and τ** are proportional to a2db for vesicles, and to a3dbdw+db for liposomes. For RDG and particle radii 20–1000 nm, τ** is proportional to an, where n is 2 to 0.4 for vesicles and 2 to 1.1 for liposomes. Turbidity data for DDAB vesicles are consistent with the RDG predictions, which are also used to estimate the vesicles’ sizes. RDG applies to liposomes < 800 nm and to much larger sizes for vesicles.

Response of nephelometric turbidity to hydrodynamic particle size of fine suspended sediment

Turbidity is used as a surrogate for suspended sediment concentration (SSC), and as a regulatory tool for indicating land use disturbance and environmental protection. Turbidity relates linearly to suspended material, however, can show non-linear responses to particulate organic matter (POM), concomitant with changes in particle size distribution (PSD). In the paper the influence of ultra-fine particulate matter (UFPM) on specific turbidity and its association with POM in suspended sediment are shown for alpine rivers in the Southern Alps of New Zealand. The approach was two-fold: a field-based investigation of the relations between SSC, POM, and turbidity sampled during event flow; and experimental work on hydrodynamic particle size effects on SSC, POM, PSD, and turbidity. Specific turbidity changes over event flow and are sensitive to increasing proportional amounts of sand, UFPM, and POM in suspension. Furthermore, the UFPM is the size fraction (<6 μm) where POM increases. The implications of the current study are that the slopes of turbidity-SSC relations are undesirable in locations that may be dominated by cyclic release of POM or distinct pulses of fine-grained material. At locations where the turbidity-SSC slopes approximate 2, the POM proportion is usually <10% of the total suspended load. However, when turbidity-SSC slopes are <1 this is likely caused by high amounts of side-scatter from UFPM concomitant with higher proportions of POM. Thus, the use of turbidity as a proxy for determining SSC may have serious consequences for the measurement of representative suspended sediment data, particularly in locations where POM may be a significant contributor to overall suspended load.

Mesohabitat current velocity effects on Didymosphenia geminata and macroinvertebrates in a SE USA hypolimnetic tailwater

The diatom Didymosphenia geminata is known to alter benthic habitat and macroinvertebrate diversity and community structure. Associations between macroinvertebrate communities and D. geminata biomass in riffle and run mesohabitats were investigated in the South Fork Holston River in Tennessee and Virginia, USA. We found that low current velocity, low turbidity, and high dissolved oxygen (DO) were strong predictors of D. geminata mat presence. Didymosphenia geminata ash-free dry mass was significantly higher in run mesohabitats with low current velocity (CV) than in riffle mesohabitats with higher CV. Macroinvertebrate alpha diversity (Shannon Diversity H’) was only marginally significantly different between riffle and runs, while beta diversity (community composition) was highly significantly different between these mesohabitats. NMDS analyses found that D. geminata was a relatively unimportant predictor of changes in community structure relative to specific conductance, CV, DO, and turbidity. However, effects of D. geminata on macroinvertebrates appear to be very taxon specific with effects on individual taxa potentially masked by tailwater effects on general macroinvertebrate diversity in global analyses. We observed that taxon-specific effects include, but are not limited to, (1) reduction of bryophyte microhabitat utilized by dominant ephemeropterans, trichopterans, amphipods, coleopterans, and some chironomid genera in run mesohabitats from competition with D. geminata for substrate attachment space; and (2) differences in utilization of D. geminata mat biomass as a food resource and microhabitat for chironomids. Our insights into taxon-specific effects of D. geminata on macroinvertebrates open up multiple avenues for experimentation in which to validate our observational findings.

A wide dynamic range and high resolution all-fiber-optic turbidity measurement system based on single photon detection technique

A high-resolution fiber-optic detection system has been developed to measure the wide dynamic range of turbidity. To obtain high-resolution measurement results, a high sensitive single photon detection technique (SPDT) has been used in the system. Based on fiber characteristics, a compact probe structure for fiber-optic integrated transceiver (FITP) is designed to transmitting and collection light signal, which has the capability of detecting turbidity in the narrow workspace. By combining the Beer-Lambert (B-L) law transmission law with single photon counting theory, a novel turbidity measurement theoretical model has been proposed, which shows a good exponential relationship over the whole measurement range from 0.01 to 1000 NTU (Nephelometric Turbidity Units). However, when detecting 0.01–0.1 NTU low turbidity, the experimental results turn into an approximate linear relationship, which good agrees with the proposed theoretical model. Last, some issues about the optimization of light intensity and requiring attention on constructing a practical distributed multi-point water turbidity remote monitoring system in the outdoors are also discussed. It was found that the system can achieve a measurement dynamic range as wide as 50 dB with a resolution better than 0.01 NTU in the range of 0.01–1000 NTU, which is suitable for some specific turbidity measurement in remote and narrow workspace.

The influence of sludge retention time on sludge flocculation in IFAS system

The IFAS system was cultivated in five sequencing batch reactors. The sludge retention times (SRT) were 6 d, 8 d, 10 d, 15 d and 25 d respectively. In this dissertation, the influence of SRT on suspended sludge flocculation in IFAS system and its mechanisms were studied. It was found that in the IFAS system, the specific turbidity of supernatant and SVI value of suspended sludge both decreased as the SRT increased. In addition, extending SRT was capable of reducing the extracellular polymeric substances (EPS) content and the interaction energy barriers, increasing the percentage of bivalent and trivalent cations in pellet, thus improved the sludge flocculation and reduced effluent turbidity.

Biodegradation of naphthalene using Pseudomonas aeruginosa by up flow anoxic-aerobic continuous flow combined bioreactor.

BackgroundNaphthalene is a poly aromatic hydrocarbon (PAH) present in many sediment-water systems. The aim of this study was to evaluate the applicability of an anoxic/aerobic system for the biological treatment of water polluted by naphthalene by Pseudomonas aeruginosa PTCC 1707 to utilize naphthalene. The naphthalene elimination from wastewater was determined in anoxic–aerobic continuous flow combined bioreactor under continuously oxic and anoxic conditions. Experiments were conducted in continues mode, and naphthalene was administered in consecutive spike doses. Then Pseudomonas aeruginosa bacteria suspension with a specific turbidity (0.5-10 NTU) was prepared from growing bacteria on R2A medium and injected to reactor.FindingsAt naphthalene concentration = 0.5-20 mg/L, 33–65.5% naphthalene removal efficiencies were observed. Mean COD removal efficiency in solid retention times of 2, 4, 6, and 8 days was 82.7, 92.45, 95.97 and 96.1%, respectively. Naphthalene removal efficiency by bacterium pseudomonas at pH 8 was 96% and at pH 4, 5.5, 7 and 9.5, 68, 80, 90 and 85%, respectively. As the initial concentration of naphthalene increased from 0.5 to 20 mg/L, the remaining concentration of naphthalene decreased from 33.4% to 65.5% after 3 days.ConclusionBased on experimental results, it was determined that this process can effectively reduce naphthalene under optimal conditions and this method can be used for the removal of similar compounds.

Application of Newton’s Zero Order Caustic for Analysis and Measurement: Part III – Light Scattering

When light is incident upon a liquid suspension that has an oblate spheroid shape, the size and presence of particles can be determined by measuring light intensity exiting the sample or by imaging the scattered light using a digital camera. This dual analysis system is facilitated by Newton’s zero order caustic formed within the sample, which creates a region of high intensity light inside the spheroid. Refraction of light exiting the spheroid directs a high percentage of scattered light near the zone of highest incident light intensity, which increases specific turbidity changes with particle size and facilitates analysis of gold nanoparticle plasmon resonance. Images taken with an ordinary digital camera at 90 degrees from the incident light can be analyzed using spatial autocorrelation in order to detect particle size. Original Research Article International Research Journal of Pure & Applied Chemistry, 4(2): 144-158, 2014 145

The Effect of Tribomechanical Micronization and Activation on Rheological, Thermophysical, and Some Physical Properties of Tapioca Starch

The aim of this research was to investigate the effect of tribomechanical treatments on rheological, thermophysical, and some physical properties of tapioca starch. Samples of tapioca starch were treated using laboratory equipment for tribomechanical micronization and activation (TMA equipment). Before and after the TMA treatment, analysis of the particle size and particle size distribution was carried out, in addition to scanning electron micrography in tapioca starch. Scanning electron micrography showed that tribomechanical processing of tapioca starch resulted in breaking accumulations of starch granules in the form of granules. Pasting parameters have shown that maximal viscosities of model starch suspension have been decreasing after tribomechanical treatment. On the basis of gelatinization curves, it can be concluded that there are changes in the gelatinization point after treatment, and there is decrease in enthalpy of gelatinization for model suspension. After tribomechanical treatment, changes in physical properties of starch suspensions were determined, as well as specific swelling capacity, solubility index, and turbidity of tapioca starch suspensions.

Anion exchange pretreatment for the removal of natural organic matter from humic rich water

The treatment of humic rich water using anion exchange, coagulation and PAC-adsorption was studied, both independently and in sequence in that order. The commercial aluminium-based coagulant PAX-16, powdered activated carbon W35 and the magnetic ion exchange resin (MIEX®) were used in the study. The source of humic substances was natural water (25.0 g C/m3 as DOC) flowing out from the Great Batorow Peat-Bog (Lower Silesia, Poland). In addition to DOC, color, UV254 absorbance, specific UV absorbance (SUVA254), turbidity, pH and alkalinity were also analyzed. Regardless of the applied technology, in the phase of raw water treatment extraordinary solutions were necessary to treat the humic rich water – a high coagulant (13.1 g Al/m3) or PAC (&amp;gt;200 mg/dm3) dose or a low bed volume (200 BVs) in the case of anion exchange. MIEX®DOC pretreatment had a very positive influence on the course and effects of coagulation resulting in a higher coagulant efficiency for removing organic compounds. In this respect, MIEX®DOC pretreatment allowed the application of a required coagulant dose four times lower compared to the coagulation of raw water. In testing the sequence of processes (MIEX®DOC – coagulation – PAC-adsorption), coagulation was the main process responsible for reducing the SUVA254 value. The improvement in water quality after PAC-adsorption was not significant, although the process removed DOC fractions that could not be removed by coagulation. Each of the applied methods played a role in the treatment of humic rich water and the nearly complete removal of NOM was achieved (in the sequence of processes) under relatively rational operating conditions for the treatment techniques.

Concentration‐Duration‐Frequency Curves for Stream Turbidity: Possibilities for Assessing Biological Impairment1

Abstract: Siltation and subsequent biological impairment is a national problem prompting state regulatory agencies to develop sediment total maximum daily loads (TMDL) for many streams. To support TMDL targets for reduced sediment yield in disturbed watersheds, a critical need exists for stream assessments to identify threshold concentrations of suspended sediment that impact aquatic biota. Because of the episodic nature of stream sediment transport, thresholds should not only be a function of sediment concentration, but also of duration and dose frequency. Water quality sondes can collect voluminous amounts of turbidity data, a surrogate for suspended sediment, at intervals that can be used to characterize concentration, duration, and frequency of elevated turbidity events. To characterize turbidity sonde data in an ecologically relevant manner, a methodology for concentration‐duration‐frequency (CDF) curves was developed using turbidity doses that relate to different levels of biological impairment. To illustrate this methodology, turbidity CDF curves were generated for two sites on Little Pigeon River in the Great Smoky Mountains National Park, Tennessee, using over 30,000 sonde data measurements per site for a one‐year period. Utilizing a Poisson arrival approach, turbidity spikes were analyzed stochastically by observing the frequency and duration of recorded events over a turbidity level that relates to a biological dose response. An exponential equation was used to fit duration and frequency of a specified turbidity level to generate concentric‐shaped CDF curves, where at specific turbidities longer durations occurred less frequently and conversely shorter durations occurred more frequently. The significance of the equation fit to the data was accomplished with a Kolmogorov‐Smirnov goodness‐of‐fit test. Our findings showed that the CDF curves derived by an exponential function performed reasonable well, with most curves significant at a 95% confidence level. These CDF curves were then used to demonstrate how they could be used to assess biological impairment, and identify future research needs for improved development of sediment TMDLs.

Scattering efficiency of a cloudy apple juice: Effect of particles characteristics and serum composition

The effect of particles and serum characteristics on turbidity of natural, or “regular”, and modeled cloudy apple juices was studied in this work. Modeled apple juices were made by re-dispersing a determined quantity of apple particles in a simplified serum (mainly glucose, hydrolyzed pectin, and malic acid in water). Only glucose was found to have a significant effect on turbidity. Particle size was affected by soluble solids concentration, which was attributed to conformational changes in juice particles aggregates, simultaneously with a reduction in particle solvatation. Scattering efficiency was determined in natural and modeled cloudy apple juice both experimentally from a nephelometric method, Qav∗, and theoretically with the Mie theory, QMie. Decrease in juice specific turbidity at increasing soluble solids concentrations (X), was governed by the decrease of Qav∗ at increasing refractive index of the liquid medium nm. As predicted by theory, the scattering efficiency increased at increasing particle size, for a constant nm. Finally, calculated values of Qav∗ and QMie followed a power law relationship when correlated. This non-linear behavior was explained by considering that QMie is the theoretical scattering efficiency of monodisperse-homogeneous spheres, while Qav∗ is the experimental nephelometric scattering efficiency of polydisperse-irregular particles.

Seasonality of Selected Surface Water Constituents in the Indian River Lagoon, Florida

Seasonality is often the major exogenous effect that must be compensated for or removed to discern trends in water quality. Our objective was to provide a methodological example of trend analysis using water quality data with seasonality. Selected water quality constituents from 1979 to 2004 at three monitoring stations in southern Florida were evaluated for seasonality. The seasonal patterns of flow-weighted and log-transformed concentrations were identified by applying side-by-side boxplots and the Wilcoxon signed-rank test (p < 0.05). Seasonal and annual trends were determined by trend analysis (Seasonal Kendall or Tobit procedure) using the U.S. Geological Survey (USGS) Estimate TREND (ESTREND) program. Major water quality indicators (specific conductivity, turbidity, color, and chloride), except for turbidity at Station C24S49, exhibited significant seasonal patterns. Almost all nutrient species (NO(2)-N, NH(4)-N, total Kjeldahl N, PO(4)-P, and total P) had an identical seasonal pattern of concentrations significantly greater in the wet than in the dry season. Some water quality constituents were observed to exhibit significant annual or seasonal trends. In some cases, the overall annual trend was insignificant while opposing trends were present in different seasons. By evaluating seasonal trends separately from all data, constituents can be assessed providing a more accurate interpretation of water quality trends.

Effect of grafted PEG on liposome size and on compressibility and packing of lipid bilayer

The aim of this study was to elucidate the effect of various mole percentages (0–25 mol%) of 2000 Da polyethylene glycol–disteroylphosphoethanolamine (PEG–DSPE) in the presence or absence of 40 mol% cholesterol and the effect of degree of saturation of phosphatidylcholine (PC) on the size and the lipid bilayer packing of large unilamellar vesicles (LUV). Egg PC (EPC, unsaturated) LUV and fully hydrogenated soy PC (HSPC, saturated) LUV partial specific volume, specific compressibility, size, and packing parameter (PP) of lipids were characterized by measurements of density, ultrasonic velocity, specific turbidity, and dynamic light scattering. Liposome size and specific turbidity decreased with increase in temperature and PEG–DSPE mol%, except at 7 ± 2 mol%. At this PEG–DSPE mol%, an anomalous peak in liposome size of 15 ± 5 nm was observed. We attribute this effect mainly to the change in the spatial structure of the PEG–DSPE molecule, depending on whether the grafted PEG is in the mushroom or brush configuration. In the mushroom regime, i.e., when the grafted PEG is up to 4 mol% in LUV, the PEG moiety did not affect the additive PP of the lipids in the bilayer, and the PP value of PEG–DSPE is 1.044; while in the brush regime, i.e., when the grafted PEG is higher than 4 mol%, the PP of PEG–DSPE decreases exponentially, reaching the value of 0.487 at 30 mol% of grafted lipopolymer. The specific compressibility and additive PP values for the mixture of matrix lipid (EPC or HSPC), cholesterol, and PEG–DSPE for all liposome compositions investigated reached their maximum at 7 ± 2 mol% PEG–DSPE, the concentration of PEG–DSPE at which the highest biological stability of the LUV is achieved.