Specific Strand Research Articles

Astrid Lindgren and the Nightingale’s Song

Theme: Motherhood and Mothering. Ill. ©Stina Wirsén This article analyzes the nightingale motif in Swedish author Astrid Lindgren’s short story “Spelar min lind, sjunger min näktergal” (“My Nightingale is Singing”), first published in her collection Sunnanäng (The Red Bird, 1959). The lineage of the motif is traced back to ancient Greek folklore, where the nightingale’s lament symbolizes maternal grief over the loss of a child. It is argued that Lindgren’s story can be interpreted as a modern reimagining of a specific strand in the mythological tradition surrounding the rape of Philomela and the infanticide committed by Procne to avenge her sister. Lindgren alludes to a version of the story found in fable collections, which centers on the reunion of the two sisters after their metamorphosis into birds. In the Greek myth, especially as it was interpreted by Romantic writers, a bereaved mother is transformed into the nightingale, eternally lamenting her loss and thereby transfiguring human suffering into beauty. In Lindgren’s story, Malin longs to bring beauty to the bleak world of the orphanage, first miraculously causing a linden tree to grow in its yard, but ultimately giving her spirit to the tree, where it is heard in the song of a nightingale among its branches. “Spelar min lind, sjunger min näktergal” thus represents Lindgren’s innovative culmination of a long fable tradition with pre-classical origins, where the child becomes the agent of transformation.

Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus

The Rabies virus, a single-strand RNA virus with a negative-sense polarity, is responsible for causing encephalitis and is a zoonotic disease. If not promptly treated after infection, it has a close to 100 % fatality rate. Similar to other negative-sense polarity single-strand RNA viruses, the Rabies virus requires the creation of a positive-strand RNA intermediate for replication. One approach to identify this replication activity is to detect the complementary strand of the viral RNA genome in suspected infected cells or tissues. The reported Rabies virus RT-qPCR detection methods are designed to detect total viral load in samples without distinguishing between positive- and negative-strand for RNA viruses. As such, in this study, a sensitive Taqman-based strand-specific RT-qPCR assay has been developed to quantitatively detect both the positive- and negative-strand of the Rabies virus. This method demonstrates good reproducibility across a wide dynamic range and exhibits linearity of 8 logs with a lower limit of detection of 103 copies/μL for the positive-strand and 9 logs with a lower limit of detection of 102 copies/μL for the negative-strand. Notably, it can accurately detect a specific viral RNA strand even in the presence of high levels of the opposite strand, confirming the method’s specificity. In summary, a reliable strand-specific RT-qPCR assay has been developed and validated to differentiate replicating from non-replicating Rabies virus.

Numerical modeling of the transfer length of 17.8 mm (0.7 in) diameter prestressing strands in pretensioned members

The bond between concrete and reinforcing steel bars in reinforced concrete structures is crucial. Prestressing strands with a diameter of 17.8 mm (0.7 in) transfer higher compression per unit area than smaller strands, improving moment capacity and shear strength and extending the girder span. However, limited research on the bond performance of these 17.8 mm diameter prestressing strands has restricted their widespread use despite their advantages. This study presents a methodology to determine the transfer length of pretensioned members numerically. The influence of pretension force and bond strength on a 17.8 mm diameter strand was assessed through the validation of a finite element model using experimental results from a standardized pretensioned pull-out test. Analytical calculations using different code formulas were also conducted, encompassing strands of varying diameters. Our finite element analysis suggests that a 50.8 mm spacing between adjacent 17.8 mm diameter strands is applicable, ensuring there is no stress overlap in the transfer zones, which can lead to concrete cracking and reduced structural integrity. Existing code formulas, such as ACI 318–19 and AASHTO LRFD 9th edition, require longer transfer lengths when compared with the numerical results. The fib MC 2010 and EC2 formulas offer closer results to numerical results of smaller diameter strands but underestimate the transfer length for the 17.8 mm diameter strand. Therefore, improvements to code formulations may be necessary for more accurate transfer length predictions for this specific strand size.

Influence of Senior High School Strand on the Academic Performance in College

The influence of a senior high school (SHS) on academic performance in college is an interesting topic that involves exploring how the specific curriculum and focus of senior high school education can impact students’ success in higher education. In this context, this study explored the influence of senior high school strands on the academic performance of Accountancy students. Data for this descriptive study were collected from 128 respondents using a 30-item, self-made data-gathering instrument that has passed rigorous tests for validity and reliability. The data show that age and senior high school strands significantly influence students’ academic performance in foundational knowledge. However, the level of influence of the SHS strand and the level of academic performance have no significant relationship. This suggests that regardless of the specific strand a student pursues during their SHS years, their performance in higher education is not directly affected by this choice. Keywords: Accountancy, senior high school strand, academic performance, Negros Occidental, Philippines

From “Let Me Show You Something Cool” to “What Do You Notice?” Preparing College Interns for Floor Facilitation in a Natural History Museum

ABSTRACT The use of facilitators to engage visitors in conversations at collections-based institutions has the potential to greatly impact and deepen visitor experience. The job requires the development of complex skills across specific strands of study. In this article, we share details on the strands of study in the training of youth floor facilitators from diverse backgrounds for work in a collections-based museum. This training program has been developed over several years. In this article, we review lessons learned, discuss issues we continue to grapple with, and recommend opportunities for further research for practitioners and researchers.

Exploring disciplinary authors’ conflict negotiation in published research article discussions

Research article (RA) discussions require authors to underline new research findings through negotiating conflict, known as academic conflict (AC), with a specific strand of literature. However, AC has been either inadequately represented in particular disciplines, or left unnoticed across disciplines in academic writing research. This study follows a cross-disciplinary perspective to examine and compare AC negotiation in published medical and applied linguistics RA discussions based on Hunston’s framework. A specialised corpus consisting of 60 RAs, 30 from each discipline, from prestigious journals was screened for AC frequency and recurrent features through manual rhetorical analysis. The findings revealed little variation in overall AC realisation across the two disciplines. In terms of AC components, medical RAs included frequent proposed claims and recurrent conflict resolution features, indicating authors’ tendency to overuse self-interpretations in arguing against previous claims. By contrast, applied linguistics authors relied more on opposed claim components to discuss new findings and resolve research tensions. These findings help enrich our understanding of AC realisations across discipline RAs, which in turn might offer helpful insights into more proportionate academic writing practice and pedagogy.

Dark-field imaging and fluorescence dual-mode detection of microRNA-21 in living cells by core-satellite plasmonic nanoprobes

The in situ precise quantification and simultaneous imaging of low abundance microRNAs (miRNAs) within living cells is critical for cancer diagnosis, yet it remains a significant challenge. Leveraging the excellent sensitivity and spatiotemporal resolution of dark-field microscopy (DFM) and fluorescence imaging, we have successfully devised a novel detection approach using dual-signal reporter probes (DSRPs). These probes allow for highly sensitive detection of miRNA-21 in living cells via toehold-mediated strand displacement cascades. The DSRPs were constructed by Au nanoparticles and Ag nanoclusters core-satellite nanostructures. After the recognition of miRNA-21, the strand displacement cascades were triggered, inducing the disassembly of the Au/Ag core-satellite nanostructure with apparent scattering intensity decrease and peak wavelength shifts. Additionally, the fluorescence of Ag clusters could be recovered and further enhanced when in close proximity to specific guanine-rich strands. The dual-signal response capability enables the accurate detection of miRNA-21 from 1 fM to 1 nM, with a limit of detection reached 0.75 fM. DFM and fluorescent imaging of living cells efficiently confirms the applicable detection of miRNA-21 in complex detection media. The biosensor based on DSRPs represents a promising nanoplatform for visual monitoring and imaging of biomolecules in living cells, even at the single particle level.

Recovery management in sport: Overview and outcomes of a nine-year multicenter research program

The nine-year multicenter research project, titled REGman—Optimization of Training and Competition: Management of Regeneration in Elite Sports—aimed to advance the understanding of recovery management in sports and generate practical recommendations. In this article, we outline the overarching research program that links the specific research strands and outputs during the two federal funding cycles (2012–2021) and summarize the main project findings. The two-stage conceptual framework involved investigating monitoring measures for short-term fatigue and recovery, as well as assessing the efficacy of various recovery interventions. These interventions encompassed psychological relaxation and recovery strategies, sleep, cooling techniques like precooling, percooling, cold water immersion, or whole-body cryotherapy, compression garments, active recovery, stretching, heating interventions such as sauna or contrast water therapy, massage, and foam rolling. The findings revealed inconclusive or marginal effects of recovery interventions at the group level, while indicating possible interindividual differences in responses. Additionally, the findings highlighted the effectiveness of diverse monitoring measures, showing satisfactory sensitivity in tracking performance changes related to fatigue and recovery. The use of individualized reference ranges significantly improved classification accuracy compared to group-based reference ranges. Athletes and coaches are encouraged to prioritize fundamental aspects of training and recovery: meticulous training planning and execution, effective sleep management, and proper nutrition. Furthermore, monitoring and analyzing individual responses, even though it demands suitable methodologies and presents challenges in high-performance sports environments, can yield valuable insights for personalized recovery management. If these aspects are comprehensively addressed, and resources allow, additional recovery strategies might be explored.

EDITING CDKN1A AND RASD1 IN A CELL CULTURE SYSTEM

Abstract As an individual ages, there is a higher incidence of cancer and infertility. Testicular germ cell cancer rates have been rising year after year. According to the American Cancer Society 1 of every 250 males will develop testicular cancer during their lifetime. In this research project, we aim to develop a molecular toolbox to analyze gene function in male germinal cells using a CRISPR/Cas9 cell culture system. This biotechnology allows for the targeted alteration and cutting of specific DNA strands. To achieve this, we will utilize cancer cell lines (SUSA and NT2/D1 testicular cancer cell lines) and knock out two genes of interest: cyclin-dependent kinase inhibitor 1 (CDKN1A) and ras-related dexamethasone-induced 1 (RASD1). Our objective is to investigate the effects of gene knockout on cell proliferation. For this purpose, we designed primers and single-guide RNAs using bioinformatic databases: Benchling and Chop Chop. The tools we used were employed to amplify human genomic DNA and assess the effectiveness of the guide RNAs through in vitro cutting of our genes of interest, followed by gel electrophoresis observations. Based on the results obtained from these experiments, our next step is to electroporate the guide RNA and Cas9 complex to alter gene function in our cell culture system. Through a better understanding of gene function in testicular cancer cell lines, we hope to inform future therapeutic targets for both testicular cancer and age-related infertility.

Assemblages, cosmopolitics and controversies: Capturing the territorial and knowledge dimensions of infrastructural politics in Lebanon

Drawing upon Actor-Network Theory literature and a specific strand of urban assemblage literature, the cosmopolitical ontology offers a fresh and innovative perspective on urban politics. It positions the interactions between human and non-human entities as the central factor shaping politics. In this article, I have adopted this cosmopolitical ontology and proposed to analyze environmental and infrastructural controversies as a crucial methodological approach. This analysis sheds light on how territoriality and knowledge, key elements of urban politics, can be understood from this perspective. I use a diversity of methods including reports and press review, interviews, focus groups and surveys to map and analyze two controversies in Lebanon around the Deir Ammar electricity plant and Saida waste management plant. I show how “rogue” material non-human actants - in the form of fumes and particles, chemical compositions of waste and composts, bacteria, fuel leaks – destabilize the territorial assemblages brought together by these infrastructures. Polluted spaces become focal points for contesting established narratives of territorial solidarity and considerably weaken institutional and political governances. Expert discourses of State institutions and technical firms are put in question. While efforts to build a “localized” knowledge empowers local actors. However, as the case studies show, when controversies become protracted, interest in the complexity of infrastructural politics dwindles and more entrenched political actors are capable of recuperating these controversies to their advantage. This is why maintaining the “territorialization” of controversies through the development of localized knowledge is essential for these cosmopolitics to bring social and political change.

Special Nuclear Structures in the Germinal Vesicle of the Common Frog with Emphasis on the So-Called Karyosphere Capsule.

The karyosphere (karyosome) is a structure that forms in the oocyte nucleus-germinal vesicle (GV)-at the diplotene stage of meiotic prophase due to the assembly of all chromosomes in a limited portion of the GV. In some organisms, the karyosphere has an extrachromosomal external capsule, the marker protein of which is nuclear F-actin. Despite many years of theories about the formation of the karyosphere capsule (KC) in the GV of the common frog Rana temporaria, we present data that cast doubt on its existence, at least in this species. Specific extrachromosomal strands, which had been considered the main elements of the frog's KC, do not form a continuous layer around the karyosphere and, according to immunogold labeling, do not contain structural proteins, such as actin and lamin B. At the same time, F-actin is indeed noticeably concentrated around the karyosphere, creating the illusion of a capsule at the light microscopy/fluorescence level. The barrier-to-autointegration factor (BAF) and one of its functional partners-LEMD2, an inner nuclear membrane protein-are not localized in the strands, suggesting that the strands are not functional counterparts of the nuclear envelope. The presence of characteristic strands in the GV of R. temporaria late oocytes may reflect an excess of SMC1 involved in the structural maintenance of diplotene oocyte chromosomes at the karyosphere stage, since SMC1 has been shown to be the most abundant protein in the strands. Other characteristic microstructures-the so-called annuli, very similar in ultrastructure to the nuclear pore complexes-do not contain nucleoporins Nup35 and Nup93, and, therefore, they cannot be considered autonomous pore complexes, as previously thought. Taken together, our data indicate that traditional ideas about the existence of the R. temporaria KC as a special structural compartment of the GV are to be revisited.

The ‘ideal victim’: A cage for victims’ narratives at the International Criminal Court

AbstractDespite Article 68(3) of the Rome Statute of the International Criminal Court (ICC) granting victims an autonomous standing in proceedings, victims’ participatory rights have often been tailored to fit within the retributive structure of the trials. This contribution aims to provide a different perspective on victims’ role and their narratives in proceedings at the ICC, building upon the expressivist model of international criminal justice and focusing on a specific strand that engages with the adjudication process’s performative and communicative features. In providing a better understanding of how victims’ narrative unfolds in trials at the ICC, the article addresses two issues: how the concept of the victim is constructed at the ICC; and whether and, eventually, how this construct impedes progress in recognizing their narratives in proceedings at the ICC. Concerning the first issue, drawing on criminologist Nils Christie’s theorizing of the ‘ideal victim’, it will be observed that the construct of victims in proceedings at the ICC reflects three main attributes: weakness; innocence; and dependency. The second issue shed light on the extent to which the emphasis on the ‘ideal victim’ can serve as a tool in the hands of institutional actors at the ICC to pre-empt, constrain and subordinate victims’ narratives, in a manner that oversimplifies victimhood. To impose a particular narrative upon victims’ experiences, three main procedural mechanisms have been identified: appropriation of victims’ interests; legal representation of abstract victimhood; and exclusion from the trial of victims who do not conform to the ideal victim.

The Possible Impact of Urgenda and the Klimabeschluss on Climate Litigation on the Example of the Petition Pending Before the Hungarian Constitutional Court

Abstract While there is scientific consensus on the causes and consequences of climate change, also reflected in international agreements and EU norms obliging states to reduce GHG emissions, domestic legislation has been slow or imperfect in implementing reduction duties. Although climate litigation is not a new phenomenon, a specific strand of climate litigation is now gaining impetus owing to the success garnered in Urgenda and the Klimabeschluss: litigation focusing on states’ omitting to lawfully regulate emission targets. This paper presents the context of novel climate litigation in the face of Russian aggression and the ensuing disincentives to promote ambitious reduction goals, proceeding to describe the arguments and findings in the two landmark cases and their effects on future climate litigation as seen in the example of the climate petition pending before the Hungarian Constitutional Court.

Assessing the early influence of COVID-19 in an analysis of the immediate implementation of Minimum Pricing for Alcohol on drinkers in Wales.

Background: The Welsh Government has commissioned a number of projects to consider the influence their implementation of Minimum Pricing for Alcohol (MPA) legislation in March 2020 had on the alcohol consumption and related behaviours of drinkers. Given the MPA's overlap with the COVID-19 pandemic and its related lockdown measures and restrictions, this rapidly became a story about the early impact of COVID-19 as it did MPA. This paper captures the core thematic messages from this specific strand of work, and in doing so reflects on (1) how early experiences of COVID-19 and the first lockdown influenced consumption and purchasing of alcohol behaviours and, in turn, (2) how relevant the introduction of MPA was for any of these. Methods: Semi-structured interviews were conducted by telephone with 32 drinkers 9 months after the implementation of the legislation in March 2020. The sample was recruited from three sources: the National Survey for Wales; a third sector organisation offering housing support to the homeless; and through an online survey on MPA. Results: COVID-19 had more relevance than MPA to drinkers. Furthermore, when MPA did have an influence on their behaviour, it was felt most keenly by the harmful drinkers in the study. These drinkers described spending more on alcohol, switching to other potentially more harmful substances, such as crack cocaine and synthetic cannabinoids, and more involvement in acquisitive crime and begging after the price increase. While our results might be an early indication of the influence of MPA on harmful drinkers, the small sample of this group in our study limits the generalisability of the findings. Conclusion: To date, the implementation of MPA has had little influence on the drinking patterns or lives of the drinkers in our sample. It is important that future research examines the longer-term influences of MPA before any conclusions on its effectiveness can be drawn.

Furan-based (photo)oxidation reactions and their application in nucleic acid and protein targeting.

Oligonucleotides (ODNs) find applications as diagnostic and therapeutic tools due to their unique ability to interact, thanks to Watson-Crick base pairing, with a specific DNA or RNA target strand. Although most of the tools available today rely on mere hydrogen bond formation, chemical modifications to enable covalent interstrand-crosslinking (ICL) have been reported, and are gaining a place under the spotlight as they potentially offer a series of advantages over the state of the art, including a higher potency and selectivity. This methodological paper focuses on the use of a pro-reactive furan moiety and its subsequent oxidation for applications in ODN targeting. The design of effective capture and targeting probes to ensure high ICL yields is discussed and the mechanisms underlying the (photo)chemical oxidation of furan are explained. Furthermore, examples of furan-containing DNAs designed for different applications, including DNA-DNA or DNA-RNA ICL and DNA-peptide/protein targeting, are provided. The paper highlights the advantages of using different oxidative chemical triggers, such as N-bromosuccinimide or singlet oxygen, to offer additional selectivity control over the ICL reaction.

Furan-modified PNA probes for covalent targeting and ligation of nucleic acids.

While natural oligonucleotides (ONs) are increasingly used as therapeutic and diagnostic tools, they still face certain challenges such as low resistance to enzymatic degradation, potential immunogenicity, and delivery issues, which can limit their applications. Peptide Nucleic Acids (PNAs) are promising alternatives due to their high affinity for DNA and RNA, the high resistance to enzymatic degradation, and the easy introduction of a wide range of potential modifications. Chemical modifications that enable the covalent targeting of specific DNA and RNA strands offer additional advantages, including enhanced potency. The current study focuses on the utilization of furan-PNAs as pro-reactive probe systems and their applications to DNA and RNA targeting. Specifically, in this methodological paper, we provide practical insights into the design, synthesis, and application of furan-containing PNA probes for achieving efficient PNA-DNA and PNA-RNA interstrand crosslinking (ICL), as well as ON-templated PNA-PNA ligation systems. Furthermore, we discuss the applications of these probes in targeting DNA secondary structures, such as G-quadruplexes and i-motifs, target pull-down assays, and on-surface detection.

COVID-19 Vaccine Hesitancy

In this article, hesitancy towards COVID-19 vaccinations is investigated as a phenomenon touching upon existential questions. We argue that it encompasses ideas of illness and health, and also of dying and fear of suffering. Building on a specific strand within anti-vaccination studies, we conjecture that vaccine hesitancy is, to some extent, reasonable, and that this scepticism should be studied with compassion. Through a mixed methods approach, vaccine hesitancy, as it is being expressed in a Swedish digital open forum, is investigated and understood as, on the one hand, a perceived need of protecting one’s body from techno-scientific experiments, and thus the risk of becoming a victim of medicine itself. On the other hand, the community members express what we call a tacit belief in modern medicine by demonstrating their own “expert” pandemic knowledge. The analysis also shows how the COVID-19 pandemic triggers memories of another pandemic, namely the swine flu in 2009–2010, and what we term a medical crisis that occurred then, due to a vaccine that caused a rare but severe side effect in Sweden and elsewhere.

O.4.6-7 The role of external providers for Physical Education and extracurricular physical activity in Irish primary schools

Abstract Purpose In Irish primary schools, Physical Education (PE) is taught by generalist teachers. While PE is critical in developing essential skills for lifelong physical activity (PA) participation, studies show teacher confidence and competence to teach PE is low due to negative personal experiences and insufficient training. Many schools utilise external providers to supplement PE provision and provide extracurricular PA. However, this provision is largely unregulated, without clear distinction between the two. This study aims to examine the role of external providers for PE and PA in Irish primary schools. Methods An online survey for principals and teachers was developed and validated using a modified Delphi technique. It included 57 questions examining current practices for PE provision schools. A link to the survey was emailed to all Irish primary schools and shared on social media. SPSS version 27 was used for data analysis. Results Of the 513 respondents, 81.7% were female, 17.9% male and 0.4% didn’t say. A majority (82%) regularly use external providers for extracurricular PA, which takes place mostly during the school day (89%). Although intended as activity not related to PE provision, it is used by teachers as the only PE provided either sometimes (45.5%) or all of the time (21.6%). External providers teach specific strands of the PE curriculum in 52.9% of schools, with Games being the strand most provided. While this provision is taking place, most teachers stay with their class and assist (61.9%). Conclusion Results show external providers are extensively used in Irish primary schools to supplement PE provision rather than solely providing extracurricular PA. Games being the most commonly delivered strand by external providers is reflective of the number of sports coaches providing this service to schools. Teachers using extracurricular PA as their PE can be problematic as external providers often lack suitable qualifications and provision may not align with PE curriculum guidelines. Support is needed for schools to provide good quality PE and approaches such as regulating the use of external providers or engaging specialist PE teachers to support delivery should be examined in order to improve PE in Irish primary schools.

Small-Molecule Analysis Based on DNA Strand Displacement Using a Bacteriorhodopsin Photoelectric Transducer: Taking ATP as an Example.

A uniformly oriented purple membrane (PM) monolayer containing photoactive bacteriorhodopsin has recently been applied as a sensitive photoelectric transducer to assay color proteins and microbes quantitatively. This study extends its application to detecting small molecules, using adenosine triphosphate (ATP) as an example. A reverse detection method is used, which employs AuNPs labeling and specific DNA strand displacement. A PM monolayer-coated electrode is first covalently conjugated with an ATP-specific nucleic acid aptamer and then hybridized with another gold nanoparticle-labeled nucleic acid strand with a sequence that is partially complementary to the ATP aptamer, in order to significantly minimize the photocurrent that is generated by the PM. The resulting ATP-sensing chip restores its photocurrent production in the presence of ATP, and the photocurrent recovers more effectively as the ATP concentration increases. Direct and single-step ATP detection is achieved in 15 min, with detection limits of 5 nM and a dynamic range of 5 nM-0.1 mM. The sensing chip exhibits high selectivity against other ATP analogs and is satisfactorily stable in storage. The ATP-sensing chip is used to assay bacterial populations and achieves a detection limit for Bacillus subtilis and Escherichia coli of 102 and 103 CFU/mL, respectively. The demonstration shows that a variety of small molecules can be simultaneously quantified using PM-based biosensors.

The Parliamentary Regime: The Political Philosophy of Confederation

AbstractThis article clarifies the intellectual origins of Canadian parliamentary government by situating Confederation within a specific strand of liberal political thought. My argument is that the Fathers of Confederation adhered to the political theory of parliamentarianism. Though liberal constitutionalists, the Fathers of Confederation expressly defended a parliamentary political framework that they considered superior to the American system of checks and balances—one characterized by a powerful elected assembly restrained by an unelected upper house, responsible ministers serving in Parliament, and a constitutional monarch. In elucidating the theory of parliamentarianism that underlies the political project of Confederation, my goal is not only to examine a problem in nineteenth-century Canadian political thought but to ground our current political situation within a larger historical perspective.