Species-specific Alterations Research Articles

Distinct metabolic responses to heme in inflammatory human and mouse macrophages – Role of nitric oxide

Activation of inflammation is tightly associated with metabolic reprogramming in macrophages. The iron-containing tetrapyrrole heme can induce pro-oxidant and pro-inflammatory effects in murine macrophages, but has been associated with polarization towards an anti-inflammatory phenotype in human macrophages. In the current study, we compared the regulatory responses to heme and the prototypical Toll-like receptor (TLR)4 ligand lipopolysaccharide (LPS) in human and mouse macrophages with a particular focus on alterations of cellular bioenergetics. In human macrophages, bulk RNA-sequencing analysis indicated that heme led to an anti-inflammatory transcriptional profile, whereas LPS induced a classical pro-inflammatory gene response. Co-stimulation of heme with LPS caused opposing regulatory patterns of inflammatory activation and cellular bioenergetics in human and mouse macrophages. Specifically, in LPS-stimulated murine, but not human macrophages, heme led to a marked suppression of oxidative phosphorylation and an up-regulation of glycolysis. The species-specific alterations in cellular bioenergetics and inflammatory responses to heme were critically dependent on the availability of nitric oxide (NO) that is generated in inflammatory mouse, but not human macrophages. Accordingly, studies with an inducible nitric oxide synthase (iNOS) inhibitor in mouse, and a pharmacological NO donor in human macrophages, reveal that NO is responsible for the opposing effects of heme in these cells. Taken together, the current findings indicate that NO is critical for the immunomodulatory role of heme in macrophages.

Assessment of PAH pollution in mediterranean lakes and health implications for fish and consumers, case study: Manzala Lake, Egypt

This study aims to investigate the distribution, sources, and compositions of 16 polycyclic aromatic hydrocarbons (PAH) in Manzala Lake water and the organs of two fish species: M. cephalus and O. niloticus. In addition, it aims to assess associated health risks and examine protein pattern responses and histopathological lesions in fish from different lake sectors. ∑PAH, ∑COMB, and ∑CAR varied between (0.122–51.895), (0.1–49.63), and (0.08–19.949) μg/L, respectively. Many PAH compounds in the water exceeded the guidelines; benzo[a]pyrene in the muscles of O. niloticus exceeded the European Union (EU) guidelines (2 μg/kg), recording 14.18 and 4.59 μg/kg in the eastern and southern sectors, respectively. In general, the ∑PAH in the muscles of O. niloticus (799.79 ± 26.02 μg/kg) were higher than those of M. cephalus (213.75 ± 8.69 μg/kg) in the eastern sector, which indicates a high potential risk associated with the consumption of O. niloticus. The electrophoresis of protein patterns of the fish muscles recorded site- and species-specific alterations in the proteinogram. M. cephalus exhibited higher sensitivity to PAH but developed a greater tolerance to PAH contamination than O. niloticus. The histopathological investigation reported several lesions in both fish tissue samples, particularly from the southern sector. Fish from Manzala Lake can pose a carcinogenic risk to consumers, mainly O. niloticus, where the PAH4 carcinogenic index was above the EU guidelines (12 μg/kg) in O. niloticus from all sectors. The study highlights lake management's importance in preserving biodiversity and ensuring food safety.

Comparative histological and ultrastructural features of the tongue of the mallard domestic duck, Anas platyrhynchos f. domestica, Anatidae (Linnaeus, 1758) in different two age stages (post-hatching [P2] and adult female) captured from Egypt.

The domestic duck is classified as a specialist filter-feeder bird living in the water. These birds also use grazing and pecking as terrestrial feeding methods. The tongues of domestic ducks, similar to those of other Anseriformes, exhibit numerous types and shapes of mechanical papillae that serve a number of purposes when collecting food. The current study attempts to describe the morphological characteristics of the tongue as well as the mechanical papillae's development. In addition, the study aims to determine whether the papillae observed post-hatching (P2) exhibit similar morphology to those found in adult female avian species, as well as to investigate the readiness of the tongue to fulfill its feeding function following hatching. The comprehensive examination of lingual mucosa is examined about the structural modifications necessary for this variety of feeding activities. In this study, the tongues of nine young (P2) and adult female were used. The tongue had three distinct parts: the apex, which had a lingual nail on its ventral surface; the body, which exhibits numerous small and large conical papillae on its lateral sides and a lingual prominence in the caudal region; and the root, which is covered with numerous conical papillae of varying sizes. Conical, filiform, and hair-like mechanical papillae, the three types of food filtration apparatus, are present in both stages. The intraoral transfer involves several structures, including the median groove, lingual combs, and the rostral border of the lingual prominence. The rostral border of the lingual prominence is characterized by distinct rows of conical papillae. The histological analysis demonstrated the presence of both keratinized and nonkeratinized epithelium on different tongue regions. The lingual salivary glands in the rostral and caudal lingual salivary glands exhibit a pronounced periodic acid-Schiff-positive reaction. Additionally, the yellow adipose tissue and sensory receptors, namely the Grandry and Herbst corpuscles, which collectively form the bill-tongue organ that monitors the movement of food. These results conclude the presence of microstructural species-specific alterations in specific tongue areas of domestic ducks' lingual mucosa. These modifications are formed by the filtering mechanism and terrestrial feeding mechanisms, such as grazing or pecking. Following hatching, the tongue of the domestic duck undergoes significant development, primarily in preparation for grazing activities. The anatomical and histological structure of the young (P2) tongue exhibited similarities to that of the adult female domestic duck while also displaying certain variations that could potentially be attributed to the bird's habitat and mode of feeding. RESEARCH HIGHLIGHTS: The results of this study concluded that the domestic duck exhibit a complex tongue structure characterized by the arrangement and morphology of its mechanical papillae, the presence of the lingual prominence with distinctive shape and the lingual comb. These features are believed to be adaptations that enable the duck to actively and efficiently filter food particles from water, serving as its primary feeding mechanism. Additionally, the tongue of domestic ducks is specifically adapted to facilitate various terrestrial activities, such as grazing and pecking. This adaptation is achieved through the presence of conical papillae and a lingual nail. These investigations facilitate our comprehension of both the anatomical and histological characteristics of the domestic duck tongue, as well as enhance our understanding of bird adaptations to various feeding mechanisms.

White-nose syndrome restructures bat skin microbiomes.

The skin microbiome is an essential line of host defense against pathogens, yet our understanding of microbial communities and how they change when hosts become infected is limited. We investigated skin microbial composition in three North American bat species (Myotis lucifugus, Eptesicus fuscus, and Perimyotis subflavus) that have been impacted by the infectious disease, white-nose syndrome, caused by an invasive fungal pathogen, Pseudogymnoascus destructans. We compared bacterial and fungal composition from 154 skin swab samples and 70 environmental samples using a targeted 16S rRNA and internal transcribed spacer amplicon approach. We found that for M. lucifugus, a species that experiences high mortality from white-nose syndrome, bacterial microbiome diversity was dramatically lower when P. destructans was present. Key bacterial families-including those potentially involved in pathogen defense-significantly differed in abundance in bats infected with P. destructans compared to uninfected bats. However, skin bacterial diversity was not lower in E. fuscus or P. subflavus when P. destructans was present despite populations of the latter species declining sharply from white-nose syndrome. The fungal species present on bats substantially overlapped with the fungal taxa present in the environment at the site where the bat was sampled, but fungal community composition was unaffected by the presence of P. destructans for any of the three bat species. This species-specific alteration in bat skin bacterial microbiomes after pathogen invasion may suggest a mechanism for the severity of white-nose syndrome in M. lucifugus but not for other bat species impacted by the disease.IMPORTANCEInherent complexities in the composition of microbiomes can often preclude investigations of microbe-associated diseases. Instead of single organisms being associated with disease, community characteristics may be more relevant. Longitudinal microbiome studies of the same individual bats as pathogens arrive and infect a population are the ideal experiment but remain logistically challenging; therefore, investigations like our approach that are able to correlate invasive pathogens to alterations within a microbiome may be the next best alternative. The results of this study potentially suggest that microbiome-host interactions may determine the likelihood of infection. However, the contrasting relationship between Pd and the bacterial microbiomes of Myotis lucifugus and Perimyotis subflavus indicate that we are just beginning to understand how the bat microbiome interacts with a fungal invader such as Pd.

Distinct impact of arbuscular mycorrhizal isolates on tomato plant tolerance to drought combined with chronic and acute heat stress

Arbuscular mycorrhizal (AM) fungi could mitigate individual drought and heat stress in host plants. However, there are still major gaps in our understanding of AM symbiosis response to the combined stresses. Here, we compared seven AM fungi, Rhizophagus irregularis, Funneliformis mosseae, Funneliformis geosporum, Funneliformis verruculosum, Funneliformis coronatum, Septoglomus deserticola, Septoglomus constrictum, distributed to many world regions in terms of their impacts on tomato endurance to combined drought and chronic heat as well as combined drought and heat shock. A multidisciplinary approach including morphometric, ecophysiological, biochemical, targeted metabolic (by ultrahigh-performance LC-MS), and molecular analyses was applied. The variation among AM fungi isolates in the enhancement in leaf water potential, stomatal conductance, photosynthetic activity, and maximal PSII photochemical efficiency, proline accumulation, antioxidant enzymes (POD, SOD, CAT), and lowered ROS markers (H2O2, MDA) in host plants under combined stresses were observed. S. constrictum inoculation could better enhanced the host plant physiology and biochemical parameters, while F. geosporum colonization less positively influenced the host plants than other treatments under both combined stresses. F. mosseae- and S. constrictum-associated plants showed the common AM-induced modifications and AM species-specific alterations in phytohormones (ABA, SA, JA, IAA), aquaporin (SlSIP1-2; SlTIP2-3; SlNIP2-1; SlPIP2-1) and abiotic stress-responsive genes (SlAREB1, SlLEA, SlHSP70, SlHSP90) in host plants under combined stresses. Altogether, mycorrhizal mitigation of the negative impacts of drought + prolonged heat and drought + acute heat, with the variation among different AM fungi isolates, depending on the specific combined stress and stress duration.

Methionine Sources Differently Affect Production of Reactive Oxygen Species, Mitochondrial Bioenergetics, and Growth of Murine and Quail Myoblasts In Vitro.

An optimal supply of L-methionine (L-Met) improves muscle growth, whereas over-supplementation exerts adverse effects. To understand the underlying mechanisms, this study aims at exploring effects on the growth, viability, ROS production, and mitochondrial bioenergetics of C2C12 (mouse) and QM7 (quail) myoblasts additionally supplemented (100 or 1000 µM) with L-Met, DL-methionine (DL-Met), or DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA). In both cell lines, all the supplements stimulated cell growth. However, in contrast to DL-Met, 1000 µM of L-Met (C2C12 cells only) or DL-HMTBA started to retard growth. This negative effect was stronger with DL-HMTBA and was accompanied by significantly elevated levels of extracellular H2O2, an indicator for OS, in both cell types. In addition, oversupplementation with DL-HMTBA (1000 µM) induced adaptive responses in mitochondrial bioenergetics, including reductions in basal (C2C12 and QM7) and ATP-synthase-linked (C2C12) oxygen consumption, maximal respiration rate, and reserve capacity (QM7). Only QM7 cells switched to nonmitochondrial aerobic glycolysis to reduce ROS production. In conclusion, we found a general negative effect of methionine oversupplementation on cell proliferation. However, only DL-HMTBA-induced growth retardation was associated with OS and adaptive, species-specific alterations in mitochondrial functionality. OS could be better compensated by quail cells, highlighting the role of species differences in the ability to cope with methionine oversupplementation.

Comparative transcriptomic analysis of long noncoding RNAs in Leishmania-infected human macrophages.

It is well established that infection with Leishmania alters the host cell's transcriptome. Since mammalian cells have multiple mechanisms to control gene expression, different molecules, such as noncoding RNAs, can be involved in this process. MicroRNAs have been extensively studied upon Leishmania infection, but whether long noncoding RNAs (lncRNAs) are also altered in macrophages is still unexplored. We performed RNA-seq from THP-1-derived macrophages infected with Leishmania amazonensis (La), L. braziliensis (Lb), and L. infantum (Li), investigating a previously unappreciated fraction of macrophage transcriptome. We found that more than 24% of the total annotated transcripts and 30% of differentially expressed (DE) RNAs in Leishmania-infected macrophage correspond to lncRNAs. LncRNAs and protein coding RNAs with altered expression are similar among macrophages infected with the Leishmania species. Still, some species-specific alterations could occur due to distinct pathophysiology in which Li infection led to a more significant number of exclusively DE RNAs. The most represented classes among DE lncRNAs were intergenic and antisense lncRNAs. We also found enrichment for immune response-related pathways in the DE protein coding RNAs, as well as putative targets of the lncRNAs. We performed a coexpression analysis to explore potential cis regulation of coding and antisense noncoding transcripts. We identified that antisense lncRNAs are similarly regulated as its neighbor protein coding genes, such as the BAALC/BAALC-AS1, BAALC/BAALC-AS2, HIF1A/HIF1A-AS1, HIF1A/HIF1A-AS3 and IRF1/IRF1-AS1 pairs, which can occur as a species-specific modulation. These findings are a novelty in the field because, to date, no study has focused on analyzing lncRNAs in Leishmania-infected macrophage. Our results suggest that lncRNAs may account for a novel mechanism by which Leishmania can control macrophage function. Further research must validate putative lncRNA targets and provide additional prospects in lncRNA function during Leishmania infection.

Association of the receptor for activated C-kinase 1 with ribosomes in Plasmodium falciparum

The receptor for activated C-kinase 1 (RACK1), a highly conserved eukaryotic protein, is known to have many varying biological roles and functions. Previous work has established RACK1 as a ribosomal protein, with defined regions important for ribosome binding in eukaryotic cells. In Plasmodium falciparum, RACK1 has been shown to be required for parasite growth, however, conflicting evidence has been presented about RACK1 ribosome binding and its role in mRNA translation. Given the importance of RACK1 as a regulatory component of mRNA translation and ribosome quality control, the case could be made in parasites that RACK1 either binds or does not bind the ribosome. Here, we used bioinformatics and transcription analyses to further characterize the P. falciparum RACK1 protein. Based on homology modeling and structural analyses, we generated a model of P. falciparum RACK1. We then explored mutant and chimeric human and P. falciparum RACK1 protein binding properties to the human and P. falciparum ribosome. We found that WT, chimeric, and mutant RACK1 exhibit distinct ribosome interactions suggesting different binding characteristics for P. falciparum and human RACK1 proteins. The ribosomal binding of RACK1 variants in human and parasite cells shown here demonstrates that although RACK1 proteins have highly conserved sequences and structures across species, ribosomal binding is affected by species-specific alterations to this protein. In conclusion, we show that in the case of P. falciparum, contrary to the structural data, RACK1 is found to bind ribosomes and actively translating polysomes in parasite cells.

Phenolic substances and cyanogenesis in galled and non-galled tissue of the fern species Microgramma vacciniifolia.

Galls, neo-formed plant structures that can occur in different organs, are generated by species-specific interaction with an inducing organism. Inducers manipulate the metabolism of its host. Microgramma vacciniifolia (Langsd. & Fisch.) Copel. is a Neotropical epiphytic fern that hosted two stem galls, one induced by a midge species (Diptera) and other by a micromoth species (Lepidoptera). The aim of this study was to evaluate the impact of these two gall-inducing insects on the biochemistry of phenolic acids and the cyanogenesis in galls, stems and leaves of M. vacciniifolia. High performance liquid chromatography (HPLC) indicated a total of 14 phenol derivatives, including caffeic and coumaric acid. Principal Coordinates Analysis (PCoA) of the phenolic substances indicated three groups consisting (1) non-galled stems and micromoth-induced galls; (2) midge-induced galls; (3) midge-induced galls with parasitoids. Regarding the frequency of cyanogenesis assessed by the picrate paper test, the chi-squared test showed significant difference between fertile leaves (8.3%), sterile leaves (27.7%), non-galled stems (0%) and galls. Among galls, only the midge-induced galls analyzed were cyanogenic (15%). Our results indicated that the different gall-inducers (midge and micromoth) promote species-specific alterations to the phenolic substance composition of the host fern.

New insights on the impact of earthworm extract on the growth of beneficial soil fungi: species-specific alteration of the nematophagous fungal growth and limitation of an entomopathogenic fungus

Earthworms influence soil ecosystem functions such as decomposition and regulation of nutrient cycles by burrowing, feeding, or secreting their cutaneous excreta. Earthworms can also modulate soil biodiversity and their interactions. Recent research has revealed that earthworm feeding activity or excretions can alter the performance of entomopathogenic nematodes (EPNs) and entomopathogenic fungi (EPFs), known biological control agents. Still, the impact of earthworm cadavers on the growth and reproduction of other biological control agents such as nematophagous fungus (NFs) and EPFs has been poorly explored. We suggest that earthworm body tissue could be used as a food supply by certain fungal species. We hypothesize that fungi with a high adaptive capacity as pathobionts such as NF will use earthworm cadavers to a certain extent, while the growth and reproduction of fungi with limited or specific nutrition needs, such as EPFs, will be restricted. In this study, we evaluated two NF species: a nematode-trapping fungus Arthrobotrys musiformis Drechsler (Orbiliales: Orbiliaceae) and a nematode egg or cyst parasite Purpureocillium lilacinum (Thom) (Hypocreales: Ophiocordycipitaceae), and one EPF species: Beauveria bassiana (Balsamo). We exposed each fungus to two different Aporrectodea molleri extract media at six concentrations in agar medium (n = 3 per treatment) and liquid medium (n = 5): (i) earthworms devoid of intestinal contents (EDG) (C1...C6), and (ii) fresh earthworms (FE) (C1...C6). The use of FE and EDG was designed to discriminate the effect of earthworms? intestinal content on fungal growth. These treatments were also compared to alternative conventional media potato dextrose agar (PDA) and brain heart infusion (BHI) as the positive controls. We evaluated the vegetative growth capacity daily for 18 days, registering the reproduction at the end of the experiment. We observed that EPF B. bassiana did not grow in FE and EDG solid medium but showed germination in their liquid media. However, both NF species could grow and reproduce in both media but following a species-specific pattern. In all cases, FE and EDG reduced fungal growth compared to PDA and BHI. Our results suggest that the use of earthworm as a resource in natural conditions might depend on the expected specialization, with EPF B. bassiana with the lowest use, NF A. musiformis moderately, and P. lilacinum widely used. The study illustrates the complexity of the soil organisms? interactions and highlights the necessity to advance the understanding of the contribution of all the beneficial soil organisms in a comprehensive manner.

Low annual temperature likely prevents the Holarctic amphipod Gammarus lacustris from invading Lake Baikal

Species with effective thermal adaptation mechanisms allowing them to thrive within a wide temperature range can benefit from climatic changes as they can displace highly specialized species. Here, we studied the adaptive capabilities of the Baikal endemic amphipods Eulimnogammarus verrucosus (Gerstfeld, 1858) and Eulimnogammarus cyaneus (Dybowsky, 1874) compared to the potential Holarctic Baikal invader Gammarus lacustris Sars, 1863 at the cellular level including the energy metabolism and the antioxidant system. All species were long-term exposed to a range of temperatures (1.5 °C to mimic winter conditions and the three species-specific preferred temperatures (i.e., 6 °C for E. verrucosus, 12 °C for E. cyaneus and 15 °C for G. lacustris). At 1.5 °C, we found species-specific metabolic alterations (i.e., significantly reduced ATP content and lactate dehydrogenase activity) indicating limitations on the activity level in the Holarctic G. lacustris. Although the two Baikal endemic amphipod species largely differ in thermal tolerance, no such limitations were found at 1.5 °C. However, the cold-stenothermal Baikal endemic E. verrucosus showed changes indicating a higher involvement of anaerobic metabolism at 12 °C and 15 °C, while the metabolic responses of the more eurythermal Baikal endemic E. cyaneus may support aerobic metabolism and an active lifestyle at all exposure temperatures. Rising temperatures in summer may provide a competitive advantage for G. lacustris compared to the Baikal species but the inactive lifestyle in the cold is likely preventing G. lacustris from establishing a stable population in Lake Baikal.

Function of pH-dependent transcription factor PacC in regulating development, pathogenicity, and mycotoxin biosynthesis of phytopathogenic fungi.

pH, as one of the most important environmental factors, affects various biological processes in pathogenic fungi. Sensing and responding to fluctuations in ambient pH are essential for these fungi to complete their life cycle. Fungi have evolved a complicated and conserved system, the so-called Pal-pH pathway, to regulate genes and adapt to alterations in ambient pH. PacC is the dominant transcription factor in the Pal-pH pathway and regulates various biological processes. The regulatory mode of PacC has been extensively studied in Aspergillus nidulans and is generally conserved in other fungal species, including numerous phytopathogenic fungi. However, species-specific alterations have been reported. This review summarizes recent advances in the regulatory mechanisms of PacC and its role in controlling development, pathogenicity, and mycotoxin biosynthesis in phytopathogenic fungi. Potential applications of these findings and some unresolved questions are also discussed.

Trophic Magnification of Short- and Medium-Chain Chlorinated Paraffins in Terrestrial Food Webs and Their Bioamplification in Insects and Amphibians during Metamorphosis.

Studies on the biomagnification of short- and medium-chain chlorinated paraffins (SCCPs and MCCPs) in terrestrial ecosystems and their bioamplification during metamorphosis in insects and amphibians are scarce. Therefore, this study sought to characterize the occurrence and trophic dynamics of SCCPs and MCCPs in an insect-dominated terrestrial food web in an e-waste recycling site in South China. Median ∑SCCPs and ∑MCCPs concentrations in the organisms ranged from 2200 to 34 000 ng/g lipid weight and from 990 to 19 000 ng/g lipid weight, respectively. The homologue profiles of CPs in the predators were distinct from those in insects, presenting more short chain-high chlorinated congeners (C10-12Cl8-10). The trophic magnification factors (TMFs) of ∑SCCPs and ∑MCCPs were 2.08 and 2.45, respectively, indicating biomagnification in the terrestrial food web. A significant positive relationship between the TMFs and octanol-air partition coefficients was observed. TMFs were also positively correlated with chlorination degree but did not correlate with carbon chain length. Nonlinear correlations between metamorphosis-associated bioamplification and the octanol-water partition coefficients of SCCPs and MCCPs were observed for insects, whereas negative linear correlations were observed for amphibians, which suggested species-specific alterations to the chemicals during metamorphosis.

Air pollution induces an adaptive response in Staphylococcus aureus and alters host-pathogen interaction

Air pollution is a major global health problem, with around 91% of the world’s population living in areas that exceed the WHO air pollution guidelines. This complex mix of pollutants almost always includes particulate matter (PM), and this has the greatest impact on human health. PM exposure contributes to a range of diseases such as COPD, heart disease and respiratory infections. Our recent publication was the first to document that as well as damaging the host, PM has a direct impact on bacteria that can cause respiratory infections. We showed that Black Carbon (BC) exposure results in species-specific alterations in biofilm structure in both Streptococcus pneumoniae and Staphylococcus aureus, altered biofilm protectivity against antibiotic exposure, and S. pneumoniae bacterial colonisation in vivo. Following on from this ground-breaking work, our current data show that the bacterial response to BC occurs at the genetic level, altering the transcription of key genes involved in biofilm formation, colonisation and virulence. Bacterial adhesion to and invasion of human epithelial cells is significantly increased when S. aureus are pre-exposed to BC prior to infection compared to naïve S. aureus cells. In a murine respiratory colonisation model, both S. aureus co-infected alongside BC, and crucially S. aureus pre-exposed to BC, show increased colonisation of the nasopharynx and lungs. These data suggest that the bacteria are responding and adapting to exposure to air pollution, and this has an impact on how the bacteria infect the host.

Host plant defense produces species-specific alterations to flight muscle protein structure and flight-related fitness traits of two armyworms.

Insects manifest phenotypic plasticity in their development and behavior in response to plant defenses, via molecular mechanisms that produce tissue-specific changes. Phenotypic changes might vary between species that differ in their preferred hosts and these effects could extend beyond larval stages. To test this, we manipulated the diet of southern armyworm (SAW; Spodoptera eridania) and fall armyworm (FAW; Spodoptera frugiperda) using a tomato mutant for jasmonic acid plant defense pathway (def1), and wild-type plants, and then quantified gene expression of Troponin t (Tnt) and flight muscle metabolism of the adult insects. Differences in Tnt spliceform ratios in insect flight muscles correlate with changes to flight muscle metabolism and flight muscle output. We found that SAW adults reared on induced def1 plants had a higher relative abundance (RA) of the A isoform of Troponin t (Tnt A) in their flight muscles; in contrast, FAW adults reared on induced def1 plants had a lower RA of Tnt A in their flight muscles compared with adults reared on def1 and controls. Although mass-adjusted flight metabolic rate showed no independent host plant effects in either species, higher flight metabolic rates in SAW correlated with increased RA of Tnt A Flight muscle metabolism also showed an interaction of host plants with Tnt A in both species, suggesting that host plants might be influencing flight muscle metabolic output by altering Tnt This study illustrates how insects respond to variation in host plant chemical defense by phenotypic modifications to their flight muscle proteins, with possible implications for dispersal.

STEM-26. ALTERED LIPID METABOLISM MARKS GLIOBLASTOMA STEM AND NON-STEM CELLS IN SEPARATE TUMOR NICHES

Abstract Clinical glioblastoma is marked by a strikingly heterogeneous mix of cell types, cellular metabolisms, and cellular microenvironments spread in different spatial locations throughout a tumor. We have created 3-dimensional organoid models that partially mimic the transition zone between nutrient-rich cellular tumor regions and nutrient-poor psuedopallisading and perinecrotic tumor zones. We found a dramatic disparity in lipid droplet presence between these regions with high lipid accumulation in the hypoxic organoid cores of a wide spectrum of patient derived specimens. This is accompanied by regionally restricted upregulation of HILPDA gene expression in the cores of our models, in clinical GBM specimens but not lower grade brain tumors, and localized specifically to pseudopallisading regions of patient tumors. We further show that lipid droplet accumulation overall marks perinecrotic and pseudopallisading regions in clinical GBM, indicating broadly altered lipid metabolism between these distinct cell populations. High lipid droplet accumulation is largely restricted to the non-stem cell populations of GBM organoids and sorted xenograft tumors whereas the stem cells are lipid-poor, suggesting lipid levels may not be simply a product of the microenvironment but also may be a reflection of cell state. We performed global lipidomic analysis on prospectively sorted stem and non-stem cells of multiple patient-derived models and found that GBM stem cells have comparatively decreased levels of neutral lipids, indicating a significant metabolic shift compared to non-stem cells from the same patient. Conversely, GBM stem cells have significantly increased levels of rare specific lipid species, and also display altered phospholipid synthesis and species specific alterations in phospholipid classes. Our findings suggest avenues for therapy by targeting the altered lipid metabolic pathways of these disparate tumor cell populations.

Gossypium barbadense and Gossypium hirsutum genomes provide insights into the origin and evolution of allotetraploid cotton

Allotetraploid cotton is an economically important natural-fiber-producing crop worldwide. After polyploidization, Gossypium hirsutum L. evolved to produce a higher fiber yield and to better survive harsh environments than Gossypium barbadense, which produces superior-quality fibers. The global genetic and molecular bases for these interspecies divergences were unknown. Here we report high-quality de novo–assembled genomes for these two cultivated allotetraploid species with pronounced improvement in repetitive-DNA-enriched centromeric regions. Whole-genome comparative analyses revealed that species-specific alterations in gene expression, structural variations and expanded gene families were responsible for speciation and the evolutionary history of these species. These findings help to elucidate the evolution of cotton genomes and their domestication history. The information generated not only should enable breeders to improve fiber quality and resilience to ever-changing environmental conditions but also can be translated to other crops for better understanding of their domestication history and use in improvement.

Species-specific alterations in Anopheles mosquito olfactory responses caused by Plasmodium infection

Mosquitoes infected with malaria parasites have demonstrated altered behaviour that may increase the probability of parasite transmission. Here, we examine the responses of the olfactory system in Plasmodium falciparum infected Anopheles gambiae, Plasmodium berghei infected Anopheles stephensi, and P. berghei infected An. gambiae. Infected and uninfected mosquitoes showed differential responses to compounds in human odour using electroantennography coupled with gas chromatography (GC-EAG), with 16 peaks triggering responses only in malaria-infected mosquitoes (at oocyst, sporozoite or both stages). A selection of key compounds were examined with EAG, and responses showed differences in the detection thresholds of infected and uninfected mosquitoes to compounds including lactic acid, tetradecanoic acid and benzothiazole, suggesting that the changes in sensitivity may be the reason for differential attraction and biting at the oocyst and sporozoite stages. Importantly, the different cross-species comparisons showed varying sensitivities to compounds, with P. falciparum infected An. gambiae differing from P. berghei infected An. stephensi, and P. berghei infected An. gambiae more similar to the P. berghei infected An. stephensi. These differences in sensitivity may reflect long-standing evolutionary relationships between specific Plasmodium and Anopheles species combinations. This highlights the importance of examining different species interactions in depth to fully understand the impact of malaria infection on mosquito olfactory behaviour.

Drawing a Line: Grasses and Boundaries.

Delineation between distinct populations of cells is essential for organ development. Boundary formation is necessary for the maintenance of pluripotent meristematic cells in the shoot apical meristem (SAM) and differentiation of developing organs. Boundaries form between the meristem and organs, as well as between organs and within organs. Much of the research into the boundary gene regulatory network (GRN) has been carried out in the eudicot model Arabidopsis thaliana. This work has identified a dynamic network of hormone and gene interactions. Comparisons with other eudicot models, like tomato and pea, have shown key conserved nodes in the GRN and species-specific alterations, including the recruitment of the boundary GRN in leaf margin development. How boundaries are defined in monocots, and in particular the grass family which contains many of the world’s staple food crops, is not clear. In this study, we review knowledge of the grass boundary GRN during vegetative development. We particularly focus on the development of a grass-specific within-organ boundary, the ligule, which directly impacts leaf architecture. We also consider how genome engineering and the use of natural diversity could be leveraged to influence key agronomic traits relative to leaf and plant architecture in the future, which is guided by knowledge of boundary GRNs.

Potential Functions of IGFBP-2 for Ovarian Folliculogenesis and Steroidogenesis.

Ovarian follicles, as transient structural and functional complexes with the oocyte and the associated cells, determine the female reproductive cycle and thus fertility. Ovarian function is subject to the strict control of hormones and growth factors and thus regulated by auto-, para-, and endocrine mechanisms but influenced also by endogenous factors. During the waves of follicular growth and development, one follicle (monoovulatory) or a limited number of them (polyovulatory) are selected under hypothalamic–gonadal control for maturation until ovulation, resulting in the fertile oocyte. Subordinate follicles inevitably enter different stages of atresia. A number of studies have observed species-specific alterations of IGFBP-2 levels during the phases of growth and development or selection and atresia of follicles. IGFBP-2 is thus probably involved in the process of follicle growth, differentiation, and degeneration. This may occur on the levels of IGF-dependent and -independent growth control but also due to the control of steroidogenesis, e.g., via induction of aromatase expression. In mice, IGFBP-2 delayed reproductive development most probably by IGF-independent mechanisms. Because reproductive development is closely linked to the control of life- or health-span and energy metabolism, we feel that the time is right now to resume research on the effects of IGFBP-2 in the ovarian follicular compartment.