Proportion Of Species Research Articles

Why are some invasive plant species so successful in nutrient‐impoverished habitats in south‐western Australia: A perspective based on their phosphorus‐acquisition strategies

Abstract Invasive plants are a major cause of the global biodiversity crisis; it is therefore crucial to understand mechanisms that contribute to their success. South‐western Australia is a global biodiversity hotspot with extremely low soil phosphorus (P) concentrations. In this region, a large proportion of native plant species release carboxylates that mobilise soil P. Many widespread invasive plant species in south‐western Australia are arbuscular mycorrhizal (AM). We hypothesised that some of these invasive AM plant species exhibit similar P‐acquisition strategies as native carboxylate‐releasing P‐mobilising species which allows them to thrive in P‐impoverished soils. To test this hypothesis, we collected 23 common invasive species in the field and assessed their leaf manganese concentration [Mn], relative to that of native reference species at different locations, as a proxy for carboxylate release. In addition, we cultivated seven of the invasive species in hydroponics at different P supply to measure their root carboxylate exudation. Furthermore, we measured leaf P concentration and photosynthetic P‐use efficiency (PPUE) of five invasive species in the glasshouse. In the field investigation, almost all invasive species exhibited significantly higher leaf [Mn] than the negative references, which do not release carboxylates, indicating carboxylate release of the invasive plants. Leaf [Mn] of a few invasives even exceeded that of positive references, which exhibit significant carboxylate release, indicating substantial carboxylate release of these invasives. All glasshouse‐grown invasive species with high field leaf [Mn] released root carboxylates under low P supply. Most of the tested invasive plant species also exhibited greater PPUE than native plants under low P supply. Invasive AM plant species exhibited root exudation of carboxylates as a P‐acquisition strategy, which very likely allows their successful invasion of severely P‐impoverished habitats. Read the free Plain Language Summary for this article on the Journal blog.

Trade-offs and management strategies for ecosystem services in mixed Scots pine and Maritime pine forests

AbstractMixed forests are increasingly recognized for their resilience to climate change and enhanced ecosystem services (ESs) provision, making them a focal point for sustainable forest management strategies. This study examines the trade-offs in ESs provision between pure and different proportions of mixed stands of Scots pine (Pinus sylvestris L.) and Maritime pine (Pinus pinaster Ait.) in the Northern Iberian Range, Spain. Using the SIMANFOR simulation platform, we evaluated various silvicultural scenarios developed to obtain different ESs such as carbon sequestration, timber and mushroom yields. Our findings reveal that ESs provision varies depending on the forest type (pure or mixed) and the mixture proportion, following different trends on each ES. The initial species proportions and their maintenance were less critical than the management approach itself, which significantly influenced ESs outcomes. Focusing solely on individual ESs can lead to trade-offs, as highlighted by our study on silviculture focused on large saw timber yields. However, adopting a balanced approach that considers multiple ESs can mitigate these trade-offs. Our findings underscore the effectiveness of this approach in maximizing yields of mushrooms, sequestered carbon, and small saw timber. This research provides valuable insights for forest managers aiming to balance productivity and sustainability in ESs provision, providing strategies to maximize compatible ESs effectively.

Assessing scientific knowledge on Ecuadorian bony fishes from a scientometric perspective.

Bony fishes play a pivotal role in Ecuador's social, economic, and ecological aspects. However, the current state of scientific knowledge on this group remains poorly understood. This study aims to assess the scientific output related to Ecuadorian bony fishes, identifying both well-researched and understudied areas. A scientometric analysis was conducted using the Scopus database, evaluating 265 peer-reviewed publications on marine and freshwater bony fishes. The results revealed that 55.5% of studies focused on marine environments, 41.9% on freshwater ecosystems, and 2.6% on mixed environments. The earliest study was published more than 130 years ago, with a marked increase in research output since 2018. Geographic and regional disparities in research efforts were evident, with the Galapagos Islands hosting the highest number of studies at the provincial level and the coastal region leading in regional publications. Additionally, there has been a notable increase in the participation of women in research over time; however, it remains significantly below parity. English was the dominant language in these publications. On average, the delay between data collection and publication was 8.43 years, which may lead to outdated or ineffective management decisions. This underscores the importance of continually updating data to ensure the accuracy of conservation status assessments. Both marine and freshwater species that were endemic have been less studied compared to non-endemic species. Most of the studied species were categorized as "least concern," although a significant proportion of species classified as "data deficient" have started to be investigated by Ecuadorian researchers. The categories "endangered" and "near threatened" were the least studied, highlighting the urgent need for conservation initiatives targeting these vulnerable taxa. Biases in the number of studies among families, species, and geographic distribution indicate the need to expand research efforts to underrepresented taxa and regions. This analysis underscores the necessity of diversifying methodologies and broadening the research focus to more comprehensively address the challenges associated with the conservation and management of bony fishes in Ecuador.

Rotifers as indicators of trophic state in small water bodies with different catchments (field vs. forest)

AbstractCurrent methods for assessing lake trophic status using zooplankton are well-established, but there is a lack of approaches for small, shallow water bodies. This study addresses the gap by analyzing rotifer communities across 100 ponds in different catchment areas (field and forest). We hypothesized that the Rotifer Trophic State Index (TSIROT) is an effective tool for assessing trophic state, yielding comparable results to the Carlson TSI index. We also proposed that variations in physical and chemical parameters would significantly affect rotifer composition, impacting the trophic state assessment. Field ponds, exposed to agricultural runoff, exhibited higher trophic states and a predominance of detritivorous species. In contrast, forest ponds, with extensive macrophyte coverage, maintained lower trophic states and supported diverse algivorous species, particularly of littoral and mesotrophic origin. This confirmed our hypothesis that catchment type strongly influences trophic state and rotifer communities. We demonstrated that rotifer traits—abundance, species diversity, habitat type preferences and proportions of eutrophic versus mesotrophic species—along with TSIROT, are effective in assessing water quality in ponds. Overall, the results validate TSIROT as a reliable tool for small water bodies, comparable to Carlson’s TSI index, and highlight the importance of catchment type in influencing water quality.

Bedrock-Dependent Effects of Climate Change on Terricolous Lichens Along Elevational Gradients in the Alps

In this study, we focused on the bedrock-dependent effects of climate change on terricolous lichen communities along elevational gradients in the Alps. In particular, we contrasted between carbonatic and siliceous bedrock, hypothesizing more favourable conditions on siliceous than on carbonatic bedrock, where dryer conditions may exacerbate the effects of climate change. To test this hypothesis, we compared terricolous lichen diversity patterns between the two bedrock types in terms of (1) species richness, (2) beta-diversity, (3) proportion of cryophilous species, and (4) functional diversity, also testing the effect of the elevational gradient as a proxy for expected climate warming. Our results indicate that the most cold-adapted part of the terricolus lichen biota of the Alps could be especially threatened in the near future, mainly on carbonatic bedrock. Actually, contrasting diversity patterns were found between carbonatic and siliceous bedrock, clearly revealing a bedrock-dependent effect of climate change on terricolous lichens of the Alps. As hypothesized, siliceous bedrock hosts a richer lichen biota than carbonatic bedrock, reflecting a general richness pattern at the national level. In general, siliceous bedrock seems to be less prone to rapid pauperization of its lichen biota, providing more suitable climatic refugia that can mitigate the effects of climate warming on terricolous lichens.

High fire frequency in California chaparral reduces postfire shrub regeneration and native plant diversity

AbstractFire is crucial for maintaining species diversity and resilience in fire‐adapted shrublands of the world's Mediterranean climate zones (MCZs), which include the chaparral shrublands of the North American MCZ. Chaparral is adapted to high‐intensity burning, with relatively long intervals between fires (30–100 years) typifying undegraded conditions. Modern fire frequencies are much higher in chaparral, driven largely by high densities of human ignitions and coincidence between ignitions and severe weather conditions. This change in the fire regime has major implications for biodiversity, leading to exotic invasion, decreased ecosystem services, and potential type conversion of shrubland to grassland dominated by exotic species. We studied the impact of increased fire frequencies on the composition and abundance of herbaceous and woody species in the Interior Coast Range of northern California. Our study area is one of the most frequently burned areas in California, which allowed us to investigate higher fire frequencies than previously published in the scientific literature for California. We surveyed fifty‐four 250‐m2 plots to assess changes in plant community composition and postfire regeneration of chaparral shrubs across a wide range of fire frequencies, including plots that have burned up to six times in the past 30 years. Our findings reveal that short‐interval fires significantly reduced postfire native woody regeneration, with obligate seeding species experiencing a 99% reduction and facultative species showing an 83% reduction in regeneration in the most frequently burned plots. Moreover, the overall marginal effect of one additional fire since 1985 decreased the proportion of native species cover by 12% and both richness and Shannon diversity by 4%. Consequently, areas with higher fire recurrence supported a more structurally and botanically homogeneous landscape dominated by a homogeneous group of non‐native species.

How do attached crown parts and branches contribute to the diversity of saproxylic fungi and beetles in downed and decaying spruce trees?

AbstractA significant proportion of forest-dwelling species in boreal forests are saproxylic, i.e., dependent on deadwood. To safeguard deadwood-associated diversity in managed forest landscapes, it is important to understand how substrate preferences and specialization structure saproxylic species communities across different deadwood resource types. In this study, we investigated the diversity and associations of saproxylic fungi and beetles at the scale of entire trees to understand how different tree parts contribute to species diversity. To do this, we sampled species assemblages in trunks (d > 15 cm), tops (d 5–10 cm) and branches (d < 5 cm) of 31 fallen Norway spruce trees. Fungal assemblages were investigated with DNA metabarcoding from wood samples, and beetles were surveyed by bark peeling and sieving. Our results showed that, fungal and beetle assemblages were clearly differentiated between trunks and branches. In the tops, fungal community composition was intermediate between trunks and branches, whereas beetle species composition was more closely aligned with trunks. Trunks and branches both harbored specialized fungal and beetle species, but no species were identified as specialists of tops. Fungal and beetle richness were lowest in branches, and fungal richness peaked in tops. Substrate specialization of saproxylic species at the scale of individual trees imply that deadwood restoration in managed forests should prioritize whole-tree retention instead of partial retention such as artificial high stumps or pruned logs.

Plant Diversity, Productivity, and Soil Nutrient Responses to Different Grassland Degradation Levels in Hulunbuir, China

Grassland degradation could affect the composition, structure, and ecological function of plant communities and threaten the stability of their ecosystems. It is essential to accurately evaluate grassland degradation and elucidate its impacts on the vegetation–soil relationship. In this study, remote sensing data based on vegetation coverage were used to assess the degradation status of Hulunbuir grassland, and five different grassland degradation degrees were classified. Vegetation community composition, diversity, biomass, soil nutrient status, and their relationships in different degraded grasslands were investigated using field survey data. The results showed that grassland degradation significantly affected the species composition of the vegetation community. As degradation intensified, species richness declined, with the proportion of Gramineae and Legume species decreasing and Asteraceae species increasing. Additionally, the proportion of annual species initially increased and then decreased. Degradation also markedly reduced aboveground, belowground, and litter biomass within the communities. Soil moisture, electrical conductivity, organic carbon, total carbon, total potassium, and hydrolyzable nitrogen contents in non-degraded areas were higher than those in severely degraded areas. Conversely, soil total phosphorus content and bulk density gradually increased with degradation. Nitrate nitrogen and ammonium nitrogen levels in severely degraded soils were significantly higher than those in non-degraded soils. Plant diversity in the study area was significantly positively correlated with aboveground biomass and belowground biomass, and it positively correlated with soil nutrient total carbon and available carbon but negatively correlated with soil bulk density. Results of the partial least squares path model showed that grassland degradation had significant negative effects on plant diversity, soil nutrients, and biomass. Soil nutrients were the main factors affecting ecosystem productivity. The direct effect of plant diversity on biomass was not significant, suggesting that soil nutrients may play a more important role than plant diversity in determining biomass during grassland degradation. The results illustrated the relationships among soil nutrients, plant diversity, and biomass in degraded grasslands and emphasized the importance of an integrated approach in the effective management and restoration of degraded grasslands.

Simulation of wetland vegetation succession based on coupled Gaussian and population dynamics models: A case study of Poyang Lake wetlands

Wetland vegetation is the most crucial primary producer in wetland ecosystems and serves as an indicator of ecosystem health. After 2003, significant changes occurred in the “river-lake relationship” between Poyang Lake and the Yangtze River, with intensified dry conditions in autumn and winter leading to a shortened inundation period for sandbanks and significant changes in the community structure and spatial distribution of wetland vegetation. By coupling a Gaussian model with a population dynamics model, this study simulated the response of wetland vegetation to inundation duration under different hydrological year types, revealing the relationship between vegetation distribution and inundation duration. Key parameters, such as growth and mortality rates, were identified, and the vegetation succession and spatial distribution of Poyang Lake wetlands were simulated for five representative years: a wet year (2010), a normal year (2001), a dry year (2006), an extreme wet year (1998), and an extreme dry year (2022). The results showed that the response of wetland vegetation to inundation duration followed a Gaussian curve, with each species exhibiting an optimal range of inundation durations. Mortality rates increased when the duration fell outside this range. In 2010, the total area of wetland vegetation was similar to that in a normal year, but the proportions of different plant species varied significantly. Compared to 2001, the areas of Phalaris arundinacea and Polygonum criopolitanum increased by 50.92 % and 24.86 %, respectively, while the areas of Phragmites australis and Triarrhena lutarioriparia decreased by 71.66 % and 83.49 %. In 1998, the total vegetation area shrank considerably, with a 54.74 % reduction compared to 2001 and a 64.32 % reduction compared to 2006. In contrast, the total wetland vegetation area reached its maximum in the extreme dry year of 2022, increasing by 41.35 % compared to 2001. Among dominant species, Carex spp., Cynodon dactylon, and Phragmites australis expanded significantly in dry years, while Phalaris arundinacea and Polygonum criopolitanum were better adapted to flood conditions.

Long-term contamination by non-native fish assemblages in a Neotropical floodplain.

Biological invasions are a major threat to biodiversity in species-rich regions. Therefore, it is important to understand mechanisms behind the long-term establishment of non-native fish species in aquatic environments in the Neotropical region. Here, we associated fish biomass, species richness, and the proportion of non-native species (contamination and Kempton's indices) to quantify the non-native pressure over fish biodiversity in lakes and rivers of the Parana River floodplain, seasonally, from 2000 to 2017. We divided species into native and non-native assemblages sampled in spatio-temporal gradients. Temporal trends were examined using linear regressions and generalised additive models. Fish biomass in gillnets increased for both native and non-native fish species, but their Kempton indices were inversely correlated. Extinction of native species occurred locally with biotic differentiation of non-native species in lakes, rivers, and ecosystem contamination. A constant increase in fish biomass resulted in overwhelming biodiversity of non-natives at the end of the time series evaluated. Native biotic resistance to introductions was not detected in deterministic trends. The observed patterns were consistent with previous studies showing native biotic homogenisation and extinction of species in response to biological invasions, landscape fragmentation, and riverine impoundments. Increases in abundance and species richness of non-native fish were the biodiversity drivers that resulted in non-native species outweighing native species in the Parana floodplain.

Native trees are responsible for the high carbon density in urban natural area forests across eight United States cities

Abstract Urban natural area forests provide cities with crucial ecosystem services, including carbon storage and sequestration. Although previous work from a single city has suggested that urban natural area forests may be carbon‐rich and dominated by native species, it is unclear if that pattern is widespread. Indeed, little is known about the species composition and carbon storage in these green spaces, or how urban natural area forests compared with similar rural forests. Here, we use data collected by the Forests in Cities network to quantify carbon stored in urban natural area forests across 1852 plots in eight of the most populous US cities and examine patterns in woody vegetation carbon storage by tree species composition and size class. We also compare the carbon storage in these urban natural area forests to similar rural forests using USDA FS Forest Inventory and Analysis data. We found that urban natural area forests store between 214 and 267 Mg C ha−1, with the majority (on average 55%) in the above‐ground portion of live trees and shrubs. This carbon was primarily stored by native trees and increased with the proportion of native species, as native trees tended to be larger. The proportion of non‐native species was higher in smaller size classes. Overall, the woody vegetation carbon density in urban natural area forests was higher than similar forest types in rural settings, a phenomenon driven by the presence of more large trees. Synthesis and applications: Together these results demonstrate the importance of urban natural area forests as carbon‐dense ecosystems and havens for native tree species. However, because of the greater number of non‐native species in smaller size classes, these forests may also be at risk of transitioning away from native‐dominated systems. Therefore, greater monitoring efforts and increased management activities such as non‐native species removal will be crucial to maintaining the high carbon storage and health of these ecosystems.

Revealing Active Sites and Reaction Pathways in Direct Oxidation of Methane over Fe-Containing CHA Zeolites Affected by the Al Arrangement.

Fe-containing zeolites are effective catalysts in converting the greenhouse gases CH4 and N2O into valuable chemicals. However, the activities of Fe-containing zeolites in methane conversion and N2O decomposition are frequently conflated, and the activities of different Fe species are still controversial. Herein, Fe-containing aluminosilicate CHA zeolites with Fe species at different spatial distances affected by the arrangement of framework Al atoms were synthesized in a one-pot manner in the presence or absence of Na. The arrangement of framework Al atoms was identified by 27Al and 29Si MAS NMR spectra and thermogravimetry-differential thermal analysis (TG-DTA) curves. Ultraviolet (UV)-vis, X-ray absorption spectroscopy (XAS), and NO adsorption fourier transform infrared spectroscopy (FTIR) spectra were adopted to analyze Fe speciation. The higher proportion of Fe species in the 6 MR of Fe-CHA zeolites in the presence of Na was confirmed by the NO adsorption FTIR spectrum. The activities of proximal and distant Fe sites in reactions including direct oxidation of methane to methanol, methanol to hydrocarbon, and N2O decomposition were compared at different temperatures to provide the corresponding active sites and reaction pathways. The distant, isolated Fe and isolated proton were more active in the direct oxidation of methane to methanol and tandem conversion of methanol to hydrocarbon reactions than the proximal, isolated Fe and paired protons, respectively. Additionally, proximal, isolated Fe sites afforded higher activity in N2O decomposition. These findings guide the design of highly active catalysts in methane oxidation, methanol to hydrocarbon, and N2O decomposition reactions, addressing energy and environmental concerns.

Identification and antimicrobial susceptibility profiles of Campylobacter isolated from camel at municipal abattoirs in eastern Ethiopia

Campylobacteriosis is an infectious zoonotic disease caused by the genus Campylobacter. The disease is transmitted from animals to humans predominantly through the consumption of contaminated food and water. However, the lack of information on the status of Campylobacter makes it difficult to quantify the role of camel meat in the dissemination of the pathogen. A cross-sectional study was conducted from June 2022 to August 2023 to investigate the occurrence of Campylobacter and associated risk factors and to determine the antimicrobial susceptibility profiles of Campylobacter species from camels slaughtered at municipal abattoirs in the towns of Harar, Babile, Jigjiga and Dire Dawa in eastern Ethiopia. A total of 324 (146 carcass swabs, 146 camel feces and 32 abattoir environmental swab samples) were collected and analyzed using TaqMan real-time PCR and culture techniques following standard procedures. In addition, antimicrobial susceptibility tests were performed using the disk diffusion method for eight antimicrobial agents according to the Clinical Laboratory Standards Institute. The overall prevalence of Campylobacter was 7.7%. Campylobacter was more frequently detected from carcasses and surface contact environmental swabs. We isolated Campylobacter at the genus level from approximately half of the PCR-positive samples, representing 54.2% (13 out of 24) of the total. The isolation levels of C. jejuni and C. coli were also 5.56% and 2.2%, respectively, which varied significantly (p = 0.037) based on sample type and site. The odds of occurrence of C. jejuni in samples collected from abattoir environments was 7.52 times greater than those in carcass and fecal samples. We detected resistance to chloramphenicol (78.6%), followed by amoxicillin (71.4%). However, 93%, 78.6%, and 71.4% of the isolates were susceptible to ceftriaxone, ciprofloxacin, and nalidixic acid, respectively. Multidrug resistance (MDR) was detected in 60% of the isolates. Of these MDR isolates, 9 (75%) were C. jejuni and 3 (25%) were C. coli. This study revealed that a considerable proportion of multidrug-resistant Campylobacter species circulate in both camel meat and abattoir environments. This indicates possible carcass cross-contamination by Campylobacter during slaughtering that can pose a threat to humans and limit therapeutic options, which could be prevented by applying good hygienic practices at abattoirs. Therefore, abattoir workers need to be aware of abattoir hygienic standard operating procedures. Regular coordinated actions should be implemented for the rational use of veterinary and medical drugs at the national level, together with training and awareness of hygienic practices.

Dieback of spruce plantations: deadwood stands and management heterogeneity enhance beetle diversity and habitat connectivity

Forest ecosystems are important for biodiversity conservation and human societies, but are under pressure due to climate change and human interventions. This applies to natural forests as well as tree plantations. The latter are globally widespread and therefore gaining increasing importance for biodiversity conservation. However, even after dieback due to increasing disturbance frequencies, such plantations are primarily managed for economic returns, leading to growing conflicts among stakeholders. In particular, the impact of forest management on biodiversity is being discussed. This study investigates the effects of five management approaches in a landscape severely affected by spruce (Picea abies L.) dieback on beetle diversity, conservation, and community composition. We considered direct effects of management and indirect effects of environmental parameters separately in ground-dwelling and flight-active beetles. Beetle diversity was strongly affected by forest management, with non-intervention deadwood stands being most beneficial for beetles. In addition, we show indirect effects of environmental factors. In general, parameters related to salvage logging (e.g. open canopies, tree stumps) influenced beetle diversity and conservation negatively, while positive effects were found for soil nutrient availability and plant species richness. Community composition differed strongly among management categories and indicated a lack of landscape connectivity for open habitat species, as we found only low proportions of such species even on salvage-logged sites. We propose a mixture of management approaches after bark beetle outbreaks, including a substantial proportion of non-intervention deadwood stands, to increase landscape heterogeneity and connectivity. This may increase overall biodiversity while addressing the concerns of both forestry and species conservation.

Features of the oropharyngeal microbiota of healthy children and those with acute respiratory infections. A prospective single-center randomized study

Background. The oropharyngeal microbiota is involved in the development of colonization resistance, affecting viral adhesion and metabolism. Any deviations in the stability of the environmental microbial niches of the oropharynx alter the local immune response and can trigger severe chronic somatic disorders. Aim. To compare the composition of the microbiota of children during an episode of respiratory infection and healthy volunteers examined in different periods of convalescence. The obtained data can be used to predict the course of the disease, analyze the risk of complications, and assess the frequency of the diseases in the future. Materials and methods. From 20.01.2022 to 23.12.2022, an open-label prospective single-center randomized comparative study was conducted, which included 120 children aged 5-10 who presented with symptoms of acute respiratory infection. The control group consisted of 15 asymptomatic children examined at different periods of convalescence. The study compared changes in the oral microbiota composition of patients and healthy children. The evaluation was performed using complete 16S rRNA gene sequencing on the Oxford Nanopore platform. Taxonometric analysis at the species and genera level was performed using the Knomics-Biota platform. The R programming language was used for statistical processing. In addition, the parameters of α- and β-diversity of the microbiota were assessed. The Chao1 and Shannon indices were calculated to assess α-diversity (diversity within one community). The Chao1 index is based on the number of species found in the sample and also rare species that are found only 1 or 2 times, thus preventing underestimation of diversity. The Shannon index includes both the number of species and their uniformity in the community. A change in the index indicates the dominance of one or more species. The following indicators were used to assess the β-diversity describing the differences between two microbiota samples at the species and genera level: Bray–Curtis dissimilarity (characterizing the ratios of common and different microorganisms between the samples) and the Aitchison distance (reflecting the differences in the proportions of microorganisms). In addition, the balance between the two groups of microorganisms was evaluated using the NearesBalance method, which characterizes the differences between the microbiota of the main and control groups. Results. The results of our study show that during respiratory infection, the state of the oropharyngeal microbiota is characterized by an increase in α-diversity, which is associated with an increase in the proportion of species of Streptococcus salivarius, Streptococcus pneumoniae, Streptococcus pseudopneumoniae, Streptococcus pyogenes, Streptococcus thermophilus and A12 versus the proportion of Streptococcus mitis, Streptococcus oralis, Streptococcus gwangjuense, Streptococcus sanguinis, Streptococcus gordonii and FDAARGOS_192. In the control group, the oropharyngeal microbiota was divided into two clusters. The dominance of streptococci was observed in the first group (control), while the second group had a more uniform representation. Analysis of the changes in microbial communities in the main and control groups can show the stages of oral microbiota recovery after an acute respiratory infection episode. Conclusion. More research is needed in the field of the oropharyngeal microbiome on a larger sample of healthy children, to standardize the communities of the oropharyngeal microbiota, to study the interaction within communities and with the body as a whole. Analysis of the impact of the microbiota on the frequency and course of respiratory infections and the rate of complications opens up new prospects in treating, rehabilitating, and preventing diseases. These research areas can contribute to improving children's health and quality of life and introducing new approaches into clinical practice.

Steppe development and mammalian adaptation in the middle Miocene, North Junggar Basin, Central Asia

The Central Asian (CA) steppe ecosystem and its fauna experienced a marked transition during the Miocene Climatic Optimum (MCO), though the exact process and dynamics remain elusive. We selected the Duolebulejin (DLB) section within the Junggar Basin to reconstruct vegetation and climate changes, using proxies such as particle size, mammalian fossils, and previously reported data including pollen, δ13CTOC, and magnetic susceptibility. The results reveal three distinct phases of transformation in regional vegetation and fauna from 18.8 to 13.2 Ma. During the pre-MCO (18.8–17.0 Ma), the landscape was dominated by desert scrubland, primarily inhabited by rodents. The MCO (17.0–14.5 Ma) saw a shift towards steppe vegetation, initially with herbaceous taxa followed by the establishment of C4 plants, alongside a diversity of large mammals adapted to open habitats. This suggests the emergence of savanna-like woodland steppes. In the post-MCO (14.5–13.2 Ma), the region transitioned to a dry steppe, with diminished animal populations, although a continued high proportion of species adapted to steppe environments. These findings underscore a co-evolutionary relationship between the development of steppe ecosystems and mammalian adaptations, driven by global MCO warming and subsequent cooling and with regional hydroclimatic changes acting as a modulating factor. The steppe-fauna biome emerged in the Junggar Basin during the MCO warming, a development whose mechanisms resemble those of tropical savannas in the late Miocene. These insights enhance our understanding of early steppe ecosystem evolution through past warming periods, shedding light on the pathways to modern steppe formation.

Canopy structure and herbage intake rate of three tropical forage grasses cultivated as pure or mixed stands

Context Using forage grass species with complementary growth and resource-use strategies to enhance forage plant diversity in pastures may be an alternative to traditional monocultures in tropical regions. Aims This study aimed to determine whether a mixture of three perennial tropical forage grasses (Andropogon gayanus cv. Planaltina, Panicum maximum cv. Massai, and Brachiaria brizantha cv. BRS Piatã) could be an alternative to enhance herbage intake rates relative to their respective monocultures. Methods The treatments corresponded to three perennial tropical forage grasses cultivated as monocultures and as a mixture composed of all three species. Defoliation management corresponded to a pre-cutting height of 35 cm and post-cutting height of 17.5 cm. Key results The botanical composition of the mixture was dynamic throughout the experimental period, with variations in the proportion of species across seasons. The upper half of the canopy predominantly comprised leaves for all treatments. The canopy structure of the mixture allowed for greater herbage intake rates than monocultures during winter/early spring. Andropogon gambagrass showed lower herbage intake rates than the other treatments. Conclusions The findings of this study indicate that it is possible to combine tropical forage grass species without compromising canopy structure and grazing animal responses, compared with single-species grass pastures. Implications The selection of forage species for mixed pastures should consider their phenological cycle, growth, and resource-use strategies to achieve temporal complementarity and provide an optimal grazing environment for animals throughout the year.

The Long-Term Dynamics of Shrew Communities: Is There a Downward Trend?

Compared to other small mammals, shrews are understudied due to their limited impact on agriculture, lower biomedical importance, and difficulty to study. Based on trapping data from 1975-2023, we investigated changes in Lithuanian shrews (Sorex araneus, Sorex minutus, Neomys fodiens, and Neomys milleri) over six decades. We analyzed the relative abundance of shrews and the proportion of their species within small mammal communities to assess temporal patterns and distribution in major habitat types. The first main finding was the confirmation of a decrease in S. araneus abundance in the 2020s compared to the 1990s and 2010s. The species proportion in 2020s was lower than in the 1970s-2000s; the decrease started in the 1980s and accelerated in the 2000s. Abundances and proportions of S. minutus and N. fodiens showed no significant trend. The abundance of N. fodiens was very low. The relative abundances and proportions of Sorex species were highest in commensal (human-related) and mixed (including forest, wetland, and meadow) habitats. Shrews were underrepresented in agricultural habitats, with the numbers of both S. araneus and S. minutus 4.1 times lower than expected. While the presence of S. minutus in commensal habitats could be explained by their diet specificity, the capture of N. fodiens and N. milleri in commensal habitats is a novel feature of their ecology.

Spatial patterns of vascular plant species richness in Poland: Relations among species group richness and hot spot distribution

Knowledge of spatial patterns of species richness (SR) is highly relevant for theoretical research in ecology and the development of conservation plans. In Poland, despite a long tradition of botanical surveys, vascular plant SR has not been mapped, nor have the correlations in richness among different plant species groups been explored at the entire country scale. Here, we used a recently published data set to examine spatial patterns and relationships among the joined SR of vascular plant species, including native species, archaeophytes, neophytes, and species with high conservation value (i.e., red-list species). The basic spatial unit employed was a 10 × 10 km grid from the Atlas of Distribution of Vascular Plants in Poland (ATPOL). We found that SR of native species and archaeophytes, neophytes, and red-list species were positively correlated. The main patterns of SR and the percentage of particular groups in the joined SR were based on three components: (1) gradient of overall SR, (2) invasion level, and (3) peculiarity of flora in some regions resulting from the high number and proportion of rare species that often have high conservation value (red-list species). In general, northeastern Poland was species-poor, while the Carpathian Mountain range, the uplands in southern Poland, and some parts of Wisła River valley had the highest SR concentrations. e location of SR hotspots usually did not overlap with the existing national parks system. The correlations among native SR, high conservation value species, and neophyte SR suggest that biological invasions are among the most important threats to vascular plant diversity in Poland. Finally, despite likely biases in SR assessments, we demonstrated that the presented maps seem to reflect general ecological gradients influencing vascular plant distribution in Poland.

Historical invasion rates vary among insect trophic groups

Globalization has spread thousands of invasive insect species into new world regions,1,2,3 causing severe losses in ecosystem services. Previous work proposed that plant invasions facilitate insect invasions through the creation of niches for non-native herbivores.3,4,5,6 Despite the impact of insect invasions, a comprehensive understanding is lacking on how invasion success varies among insect feeding groups. We therefore compiled the predominant larval trophic groups (herbivores, predators, parasites, detritivores, and brood-carers) for 5,839 non-native insect species in nine world regions to compare (1) proportions of species in each group between non-native species and the world's fauna, (2) how invasion success for each trophic group has changed over the last three centuries, and (3) how historical herbivore invasions are related to plant invasions over time and parasite invasions are related to herbivores. We find that herbivores represent a significantly larger proportion (52.4%) among non-native insects compared with the world fauna (38.4%), whereas proportions of non-native detritivores (including fungivores), predators, and brood-carers are significantly lower; parasite proportions do not significantly differ. Predators and detritivores dominated among invasions in the 18th century but subsequently diminished, likely due to changing invasion pathways, whereas proportions of herbivores, parasites, and brood-carers increased over time. We found herbivore invasions to lag 80 years behind plant invasions, whereas parasitoids appear to co-invade with their herbivore hosts. The dominance of herbivores among non-native insects and their strong cross-correlation with plant invasions further strengthens the hypothesis that plant invasions drive the global rise in numbers of non-native insects.