The river Chenab, river of the Indian sub-continent in north-western India and north-eastern and eastern Pakistan, has mostly torrential flow in India. It drains Jammu region of the Union Territory of Jammu and Kashmir, India, and after passing through Pragwal wetland, Akhnoor, enters into the broad alluvial lowlands of Punjab province, Pakistan. The present communication highlights the origin and seasonal qualitative and quantitative fluctuations of zooplankton of the Himalayan river Chenab at Akhnoor, Jammu. Zooplankton of the river Chenab has its origin mainly in rockpools, in tributaries joining upstream Akhnoor and a part is periphytonic (epilithic/ epibenthic). A total of 28 zooplanktonic species belonging to 25 species of protozoa, gemmules of 2 species of Porifera (Spongilla lacustris and Eunapius carteri) and 1 species of Turbellaria (Planaria sp.) were noticed. There is almost no change in composition of zooplankton from the earlier observations at Akhnoor. Whereas, its slow moving and polluted segment in Pakistan is dominated by metazoans. Qualitatively and quantitatively zooplankton remained irregular and low during monsoon and post monsoon months. Analysis of coefficient of correlation of zooplankton with water quality parameters is almost insignificant.