Origanum Species Research Articles

Identification of compounds from Origanum compactum and Origanum elongatum using HPLC/UV-ESI-MS and comparative analysis of their antioxidant, antimicrobial, anticoagulant, and antidiabetic properties

The aim was to assess the phytochemical composition, phenolic component levels, and biological properties of the flowering tops of Origanum compactum and Origanum elongatum. The study employed phytochemical assays, spectrophotometric techniques for quantitative analysis of polyphenols, flavonoids, and tannins, and compound identification using HPLC/UV-ESI-MS. The antimicrobial, antioxidant, anticoagulant, and antidiabetic properties were examined both in vitro and in vivo. The results showed that the O. compactum extract had significantly high levels of total polyphenols, measuring 47.368 mg gallic acid equivalents per gram, and flavonoids, measuring 14.839 mg quercetin equivalents per gram. The phytochemical examination of O. compactum revealed that lithospermic acid accounted for 36.82 % of the chemicals detected, followed by salvianolic acid C at 12.57 % and ros-marinic acid at 6.01 %. The main constituents of O. elongatum are salvianolic acid C (14.46 %), luteolin-3-O-glucuronide (13.51 %), salvianolic acid B (12.24 %), rosmarinic acid (7.83 %), and rutin (6.18 %). The results demonstrated different levels of effectiveness against the investigated microorganisms, with the extract from O. compactum exhibiting better activity, particularly against Gram-negative bacteria, certain yeasts, and the fungus Aspergillus niger. The aqueous extracts of both Origanum species demonstrate significant antioxidant activity. O. compactum has a higher total antioxidant capacity (IC50 of 35.083 μg/mL) compared to O. elongatum (IC50 of 77.080 μg/mL). However, O. elongatum has a higher reducing power (35.697 μg/mL) compared to O. compactum (42.563 μg/mL). In vivo evaluations revealed that the aqueous extracts of O. compactum and O. elongatum possess significant antihyperglycemic and anticoagulant properties. The extracts demonstrated a marked reduction in blood glucose levels during the oral glucose tolerance test (OGTT) in Wistar rats and effectively prolonged both prothrombin time (PT) and activated partial thromboplastin time (aPTT), highlighting their ability to inhibit coagulation pathways. Moreover, their comparable efficacy to standard antihyperglycemic medications and absence of severe toxicity, even at high doses, underscore their therapeutic potential for safe and effective treatment applications. Between the two species, O. compactum exhibited superior efficacy in key biological activities such as antioxidant, antimicrobial, and anticoagulant properties, making it a strong candidate for therapeutic applications. This study underscores the value of Origanum species as a rich source of bioactive compounds, offering significant potential in pharmaceuticals, nutraceuticals, and agri-food industries. The findings pave the way for further exploration of their diverse applications.

Determination of Nutrient Elements Content, Essential Oils Ratio and Biochemicals Composition of Origanum rotundifolium Boiss. and Origanum syriacum L.

In the study, the biochemical composition of bioactive ingredients, essential oils ratio and compounds, mineral elements content, and antioxidant activities of two Origanum species (O.rotundifolium Boiss. and O. syriacum L.) were investigated. It has been observed that the total flavonoid and phenolic contents vary, with the amounts being 6.57 mg QE g-1 and 225.79 mg GAE g-1 for O. rotundifolium Boiss., and 184.65 mg QE g-1 and 114.46 mg GAE g-1 for O. syriacum L., respectively. The highest element contents have been determined for macroelement Calcium (Ca)> Potassium (K)> Magnesium (Mg); and for microelement Iron (Fe)> Manganese (Mn) > Zinc (Zn)> Copper (Cu)>, respectively. The essential oil yield was determined between 2.39% (O. rotundifolium Boiss) and 5.29% (O. syriacum L.). Essential oil compositions were determined by GC-MS (Gas Chromatography–Mass Spectrometry analysis). Besides, the major components found in two species were carvacrol, γ-terpinene and cymene. As a result of the study, O. rotundifolium Boiss. and O. syriacum L. species are rich in nutrients and biochemical content and can be used in many areas as an alternative food source.

Novel Blood Pressure-Lowering Effect of Spathulenol in Rats

In recent years, natural compounds from plant sources are sought after as alternative anti-hypertensive treatments. Spathulenol (Spa), a terpenoid plant metabolite obtained mainly from the Origanum species has shown anecdotal evidence of blood pressure (BP)-lowering properties probably through the effects on vasoreactivity. This study aimed to investigate the BP-lowering activities of Spa in vivo as well as to ascertain the mechanisms of action in vitro. Male Sprague-Dawley (SD) rats (n=16) and spontaneously hypertensive rats (SHR) (n=16) with their normotensive Wistar-Kyoto (WKY) rats (n=16) as controls were subjected to anaesthesia and administered intravenous boluses of Spa at 0.0, 0.045, 0.90, 0.18, 0.36, and 0.7 mg/kg or positive control losartan at 0.0, 0.6, 1.2, 1.8, 2.4 and 3.0 mg/kg. The BP and heart rate (HR) of the rats were recorded by a computerised physiographical system (Power Lab) through carotid arterial cannulation that was connected to a pressure transducer. The data were analysed by using the Lab Chart 6 software. Spathulenol was also tested for angiotensin-converting enzyme (ACE) inhibitory activity by using the ACE1 inhibitor screening kit (PromoKine). Spathulenol was able to decrease the BP of rats in a dose-dependent manner with the BP-lowering effect being significantly (p < 0.05) higher in the SHRs than in the WKY controls. Furthermore, Spa was able to inhibit the ACE activity suggesting the possibility that this could be one of the mechanisms underlying the observed BP-lowering effect.

High cross-pollination rate of Greek oregano (O. vulgare ssp. hirtum) with Common oregano (O. vulgare ssp. vulgare) under open field conditions as revealed by microsatellite marker analysis

We studied the mode of pollination in Greek oregano (Origanum vulgare ssp. hirtum) under both controlled and open pollination conditions. When grown indoors without the presence of insects, Greek oregano plants did not develop any seeds, indicating a low level of spontaneous self-pollination. Applying manual self-pollination under the same conditions resulted in only 16 seeds, of which only five were able to germinate. At the same time, a clonally propagated Greek oregano plant of the same genotype produced a rich set of over 300 seeds in open field conditions when the flowers were visited by insects in an area where no other Origanum species were observed. Analysis with SSR markers showed that over 70% of the seeds likely resulted from self-pollination, indicating that insect-mediated pollination is essential for the seed development. We further analyzed the cross-pollination of Greek oregano with Common oregano (O. vulgare ssp. vulgare) in open field conditions where the two subspecies were grown in close proximity. Applying SSR markers, we analyzed 83 plants obtained from seeds of three vegetatively propagated Greek oregano mother plants. Surprisingly, the results showed that all analyzed seedlings resulted from cross-pollination of Greek oregano with Common oregano, indicating that cross-pollination between the two subspecies can completely take over the self-pollination or cross-pollination between the Greek oregano plants. The possible impact of the observed high cross-pollination rate on the genetic origin of seeds of selected Greek oregano lines and varieties, as well as on the genetic diversity and structure of natural populations, is discussed.

From the wild to the field: effect of foliar or soil application of inorganic or semi-organic fertilizers on various parameters of four local endemic plant species of Crete (Greece)

Domestication of wild plant species of interest coupled with introduction of pilot cultivation practices and appropriate fertilization schemes could be an efficient alternative for addressing sustainable exploitation needs of threatened and/or declining wild phytogenetic resources. In this field study focusing on four threatened wild-growing plants (Carlina diae, Origanum dictamnus, Origanum microphyllum, and Sideritis syriaca subsp. syriaca) confined to Crete (local endemics), the effects of three types of fertilization (inorganic, plain semi-organic or co-applied with commercial biostimulants based on edible raw plant materials) by two methods (foliar/soil application) on plants’ growth were investigated. Specifically, plant parameters such as aboveground biomass yield, content and uptake of nutrients by plants as well as arbuscular mycorrhizal fungi root colonization were evaluated. Results revealed a distinction in plants’ multi-elemental stoichiometry, except in the case of Origanum species, in which the variance of their ionomics showed a significant overlap. The elements most closely related to yield were K and Zn, while Pearson tests showed various correlations between studied essential nutrients. Among them, the positive correlations between B and Ca, and Mg (with r value up to 0.9 at p ≤ 0.001, in all studied species except O. dictamnus); the negative correlations between K and Mg (in O. dictamnus and S. syriaca subsp. Syriaca, r = − 0.5 at p ≤ 0.001); and the positive correlation patterns between Fe and Mn, or Cu and Zn, respectively (in all studied species) , were observed. Biostimulant application alone or in combination with semi-organic fertilizers increased the yield of C. diae (up to 161%) and of the two Origanum species studied (up to 70% and 68% for O. dictamnus and for O. microphyllum, respectively), while inorganic fertilization was beneficial for S. syriaca subsp. syriaca (up to 170% increase) and O. microphyllum (up to 79% increase). However, no solid conclusions could be derived in respect of the preference of the four species for any fertilizers' application method (foliar or by soil).

Microbiological Studies on the Influence of Essential Oils from Several Origanum Species on Respiratory Pathogens.

Essential oils (EOs) with established and well-known activities against human pathogens might become new therapeutics in multidrug-resistant bacterial infections, including respiratory tract infections. The aim of this study was to evaluate the antimicrobial activity of EOs obtained from several samples of Origanum vulgare, O. syriacum, and O. majorana cultivated in Poland. EOs were analyzed by GC-MS and tested against four bacterial strains: Staphylococcus aureus (MRSA), Haemophilus influenzae, Haemophilus parainfluenzae, and Pseudomonas aeruginosa. Chemical analyses showed that the Eos were characterized by a high diversity in composition. Based on the chemical data, four chemotypes of Origanum EOs were confirmed. These were carvacrol, terpineol/sabinene hydrate, caryophyllene oxide, and thymol chemotypes. Thin-layer chromatography-bioautography confirmed the presence of biologically active antibacterial components in all tested EOs. The highest number of active spots were found among EOs with cis-sabinene hydrate as the major compound. On the other hand, the largest spots of inhibition were characteristic to EOs of the carvacrol chemotype. Minimal inhibitory concentrations (MICs) were evaluated for the most active EOs: O. vulgare 'Hirtum', O. vulgare 'Margarita', O. vulgare 'Hot & Spicy', O. majorana, and O. syriacum (I) and (II); it was shown that both Haemophilus strains were the most sensitive with an MIC value of 0.15 mg/mL for all EOs. O. majorana EO was also the most active in the MIC assay and had the highest inhibitory rate in the anti-biofilm assay against all strains. The most characteristic components present in this EO were the trans-sabinene hydrate and terpinen-4-ol. The strain with the least sensitivity was the MRSA with an MIC of 0.6 mg/mL for all EOs except for O. majorana, where the MIC value reached 0.3 mg/mL. Scanning electron microscopy performed on the Haemophilus influenzae and Haemophilus parainfluenzae biofilms showed a visible decrease in the appearance of bacterial clusters under the influence of O. majorana EO.

Essential oil of Origanum minutiflorum exhibits anti-inflammatory and antioxidative effects in human bronchial cells and antimicrobial activity on lung pathogens

IntroductionOregano essential oil (OEO) is one of the most widely used essential oils worldwide due to its huge therapeutic benefits. Nevertheless, data on the effects of the endemic species Origanum minutiflorum, also known as wild or Turkish oregano, is scarce. On the other hand, various chronic lung diseases, characterised by persistent inflammation, oxidative stress, and common bacterial infections, do not have effective pharmacological therapy. Hence, the aim of this study is to examine the effects of wild oregano essential oil (WOEO) on human bronchial epithelial cells and lung pathogens. MethodsWe provided a detailed chemical composition of WOEO using GC-MS and GC-FID analysis. Anti-inflammatory effects of WOEO were analysed using the qRT-PCR and ELISA methods, while antioxidative properties were examined by using the dichlorofluorescein assay in BEAS-2B cells. Antibacterial activity was tested on lung pathogens by using the agar diffusion assay. ResultsThe major constituents of WOEO, analysed in this study, were carvacrol, linalool, p-cymene, γ-terpinene, and (E)-caryophyllene. We found that treatment with WOEO attenuated LPS-induced IL8 gene expression and hydrogen peroxide-induced oxidative stress in BEAS-2B cells. Moreover, WOEO showed an inhibitory effect on pathogenic bacteria Acinetobacter baumannii and highly resistant Klebsiella pneumoniae commonly seen in healthcare-associated pneumonia. ConclusionOur work presents new insights into the anti-inflammatory, antioxidative, and antimicrobial properties of WOEO which may be used as a simple and local treatment in various chronic lung diseases.

The effect of foliar application of Titanium dioxide nanoparticle on the physiological and antioxidant performance of two Origanum species

The effect of foliar application of Titanium dioxide nanoparticle on the physiological and antioxidant performance of two Origanum species

A Set of Highly Polymorphic Microsatellite Markers for Genetic Diversity Studies in the Genus Origanum

This study reports the development of a set of 20 highly polymorphic genomic SSR markers which can be used for both cultivar identification and genetic diversity studies in several Origanum species, including some of the most popular ones like Greek oregano (Origanum vulgare L. ssp. hirtum), common oregano (O. vulgare L. ssp. vulgare), and sweet marjoram (O. majorana L.). Analysis of the polymorphic information content (PIC) showed an average PIC value of 0.75 with a minimum of 0.41 and a maximum of 0.89, where 17 of the markers showed PIC values above 0.73. Comparative analysis of the genetic diversity of eight natural populations of Greek oregano in Bulgaria showed that six of the genomic SSR markers revealed significantly higher portions of genetic diversity in the populations, compared to 12 EST SSR markers used in our previous study. We also compared the performance of the same six genomic SSR markers with the results for eight SRAP primer combinations, which showed that SRAP markers captured more precisely the genetic structure in natural populations. The developed highly polymorphic genomic SSR markers can be successfully applied to evaluation of the genetic diversity in the genus Origanum, based on the expected and observed heterozygosity in the populations as well as for easy identification of breeding lines and cultivars based on unique SSR fingerprints.

Essential oils of Origanum Species from Turkey: Repellent Activity Against Stored Product Insect Pests

Studies on the production of plant-based pest control strategies have been growing in recent years. Origanum (Lamiaceae) species are important medicinal aromatic plants and many studies have been conducted on their biological activities. This study was conducted to determine the repellent effects of plant essential oils extracted from four different Origanum species: O. onites, O. vulgare var. hirtum, O. vulgare var. verticium and O. onites × O. vulgare, against four different stored product pests: Rhyzopertha dominica (F., 1792) (Coleoptera: Bostrichidae), Tribolium confusum Jacquelin Du Val, 1863 (Coleoptera: Tenebrionidae), Sitophilus granarius (Linnaeus, 1875) and Sitophilus oryzae (Linnaeus, 1763) (Coleoptera: Curculionidae), under laboratory conditions. The neo-clavenger apparatus was used to obtain essential oils. As a result of the experiment, O. onites essential oil showed the highest activity at a dose of 0.25 μl cm-2 with 68% on T. confusum after 2 hours. This oil showed the highest activity on S. oryzae with 90% at the lowest application dose of 0.025 μl cm-2. When the results are evaluated generally, the highest activity was found in O. onites essential oil. Other essential oils have varying degrees of activity depending on time and dose. Results of the experiment show that Origanum oils have a significant potential to controlling this pest.

Bioautography for evaluation of several lavandula l. and origanum species antimicrobial and antioxidant activity

Bioautography for evaluation of several lavandula l. and origanum species antimicrobial and antioxidant activity

Quantitative Analysis of Bioactive Compounds by High‑Performance Liquid Chromatography in Origanum bilgeri

Origanum genus is an aromatic and medicinal plant with significant bioactive compounds. Origanum species have been used as a folk medicine for years. In this study, the aerial part of Origanum bilgeri P.H. Davis was extracted with hexane and methanol successively, the methanol extract was used for quantitative analysis of bioactive compounds by High‑Performance Liquid Chromatography. Rosmarinic acid was found as a major product (26.61 mg/g extract). The other major compounds were detected as catechin hydride (5.65 mg/g extract), trans-cinnamic acid (2.65 mg/g extract), and resveratrol (1.33 mg/g extract). Consequently, Origanum bilgeri may be a valuable material for drug development and food.

Phytotoxic Effects of Three Origanum Species Extracts and Essential Oil on Seed Germinations and Seedling Growths of Four Weed Species

The use of chemical pesticides to protect agricultural products is a global concern because of their adverse effects on the environment and public health. To avoid the dangers of synthetic herbicides, research has turned to natural alternatives. This study was conducted to evaluate the allelopathic effect of essential oil (EO) extracted from Origanum syriacum, Origanum onites, and Origanum majorana. In addition, the chemical composition of the essential oil was elucidated by gas chromatography and mass spectrometry (GC–MS) analysis. A total of 11 different components of O.syriacum were identified, and the main components were carvacrol (88.49), p-Cymene (5.71), γ-Terpinene (1.63), β-Caryoplhyllene (1.48), and Terpinen-4-ol (0.65), respectively. For O. onites, 10 different compounds were identified, and the main components were carvacrol (58.65), Thymol (30.97), Linalool (4.17), p-Cymene (1.94), and β-Caryoplhyllene (0.98), respectively. Finally, for O. majorana, 14 different compounds were identified, and the main components were carvacrol (40.57), α-Terpineol (29.28), p-Cymene (9.02), γ-Terpinene (5.80), and carvacrol methyl ether (3.46). Finally, 14 compounds from the Origanum majorana species were identified, with carvacrol (40.57), -Terpineol (29.28), p-Cymene (9.02), and -Terpinene (5.80) as the parent compound (3.46). Oxygenated monoterpenes were the highest in all species’ EO content. EOs and plant extracts were tested at 5, 10, and 20 L/Petri concentrations against seed germination and seedling growth in four weed species (Thlaspi arvense, Amaranthus retroflexus, Rumex cripus, and Lactuca serriola). The concentrations of essential oil were set as 5, 10, and 20 µL/Petri dishes for seed germination. In the greenhouse experiment, the final concentration of solutions was set as 20 µL and the solutions were directly sprayed on the surface of the weeds, and the mortality rates were noted after 24 and 48 h of application. It was observed that increasing the application decreased seed germination. The phytotoxic effects on the seedling germination in the greenhouse were observed, resulting in 48.76–94% mortality rates. Consequently, the essential oil from Origanum species could be considered as an alternative bio-herbicide to tested weeds.

Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines

ABSTRACT Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.

Synergistic larvicidal and repellent effects of essential oils of three Origanum species on Rhipicephalus annulatus tick.

Ticks are of great economic importance worldwide, both because they represent major obstacles to livestock productivity and because of their ability to transmit diseases to humans and animals. Although synthetic acaricides are the most common method for tick control, their overuse has led to the development of resistance as well as unacceptable residual levels in animal products and in the environment in general. There is therefore an urgent need to identify alternative treatments. Among such alternative approaches for tick control is plant essential oil (EO) therapy. In the present study, we investigated the synergistic effect of EOs of three oregano species-Origanum onites, O. majorana and O. minutiflorum-against Rhipicephalus annulatus larvae. Gas chromatography-mass spectrometry profiles of the three EOs revealed that carvacrol was their major component, with a concentration of 86.2% in O. majorana, 79.1% in O. minutiflorum and 77.4% in O. onites. The results of larvicidal assays revealed that the doses that lead to the death of 50% of the ticks (LC50) were 22.99, 25.08 and 27.06 µL/mL for O. majorana, O. minutiflorum and O. onites EOs, respectively, whereas the doses that lead to the death of 99% (LC99) were 41.26, 43.62 and 48.96 µL/mL. In addition, the LC50 and LC99 of the three oils combined was lower (viz., 4.01 and 6.97 µL/mL) than that of each oil alone. The tested EOs were also able to repel larvae of R. annulatus to varying degrees, with O. onites oil exhibiting the greatest repellent effect, as shown by the lowest RC50 dose, followed by O. minutiflorum and O. majorana. Interestingly, this means that the oil that was least effective in killing the larvae was the most effective in repelling them. The calculated synergistic factor of any combination was higher than 1 which means that combinations have a synergistic effect. In conclusion, the combination of all three oils showed higher toxic and repellent activities than either oil separately or combinations of any two oils, suggesting synergistic effects with low doses. Further studies including field trials and the establishment of the mode of action and side effects are urgently needed to expand on these findings, and other tick stages such as adults should also be tested.

Antibacterial Potency for Mimosa, Quebracho and Essential Oils of Origanum Species against Acinetobacter pittii, Klebsiella pneumonıae and Bacillus cereus from Diabetic Foot Patient

The diabetic patients may sometime suffer from foot lesions, foot ulcers and amputation that adversely affect their quality of life. In this respect, good footwear has a critical significance for diabetic foot patients. The aim of this study to evaluate the potential efficacy of mimosa, quebracho, and essential oils of Origanum onites, Origanum onites oleum, and Origanum minutiflorum, against Acinetobacter pittii, Bacillus cereus, and Klebsiella pneumoniae, which was isolated from diabetic foot patient. According to our results, the mimosa extracts were slightly more efficient when compared to quebracho extracts to control the bacterial growth of A. pittii (8.77±0.58-35.12±8.41% inhibition for three doses), and K. pneumoniae (21.40±0.48-47.04±0.51% inhibition) except B. cereus (71.1±0.31-23.51±1.66% inhibition). But these inhibition percentages remained at lower levels. On the other hand, essential oil samples of O. onites, O. onites oleum, and O. minutiflorum at tested doses have considerably high antibacterial effects against A. pittii, B. cereus, and K. pneumoniae. The tested essential oils almost completely inhibited B. cereus with percentages of inhibition ranging from 96.18±2.98-100±0.00. Also, the bacterial growth of K. pneumoniae and A. pittii was inhibited by 88.01±2.36 to 100±0.00% and 71.42±12.57 to 100±0.00%, respectively. Moreover, the essential oils of O. onites, O. onites oleum, and O. minutiflorum, had bactericidal activity against A. pittii, and K. pneumoniae but bacteriostatic activity against B. cereus. This potency for essential oil of Origanum species may be evaluated for diabetic footwear. More detailed technical studies are required for the application of Origanum species to leather footwear.

Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery.

Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13±33.8µmol/L (mean±standard error of the mean-SEM). At 300µmol/L, CRV shifted downward the 5-HT concentration-response curve with a statistical significance of p<0.001 obtained for the four highest concentrations. At a concentration of 1mmol/L, CRV completely abolished BaCl2-induced contraction in Ca2+-free Krebs-Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs-Ringer bicarbonate solution (p<0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of CRV (p<0.001). The blocking of L-type Ca2+ channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV3 channels in CRV-induced vasodilation of HUA cannot be excluded either.

Chemical compositions and in vitro antifungal activities of essential oils obtained from different Origanum species against postharvest gray mold rot of persimmon fruit

ISHS VII International Symposium on Persimmon Chemical compositions and in vitro antifungal activities of essential oils obtained from different Origanum species against postharvest gray mold rot of persimmon fruit

Multidimensional exploration of essential oils generated via eight oregano cultivars: Compositions, chemodiversities, and antibacterial capacities

Numerous species of Origanum (Lamiaceae) have been widely used as spices to extend the shelf life of foods. Essential oils extracted from this genus have attracted much attention owing to their potential applications as bactericides. Here, we evaluated the chemical compositions of eight oregano essential oils (OEOs) using gas chromatography-mass spectrometry and assessed their antibacterial activities. The chemical compositions of OEOs were affected by the cultivar factor, and seven common compounds, including carvacrol, were identified among eight OEOs. Partial least squares discriminant analysis enabled the distinction of three groups among these OEOs, as characterized by the proportions of carvacrol, thymol, and sesquiterpenes. OEOs effectively inhibited Escherichia coli and Staphylococcus aureus with varying antibacterial activities. Spearman correlation network highlighted core antibacterial contributors in the chemical profiles of OEOs. Our results revealed that the bacteriostatic effects of OEOs could be explained by core compounds and their synergistic effects.

Effect of substrate type and cultivation site in the urban fabric on growth and safety to consume the edible medicinal species Origanum dictamnus

ISHS IV International Symposium on Horticulture in Europe - SHE2021 Effect of substrate type and cultivation site in the urban fabric on growth and safety to consume the edible medicinal species Origanum dictamnus