Leafy Vegetable Species Research Articles

Phytodiversity of Species Used to Prepare "Tchonron" Sauce, A Cultural Dish of The Senoufo People, Northern Côte d’Ivoire

The Sénoufo, a people from the north of Côte d'Ivoire, are renowned for their consumption of leafy vegetables, particularly in the little-known ‘TCHONRON’ sauce. The aim of this study is to draw up a list of the leafy vegetables used in the preparation of this dish, in order to enhance their value and contribute to the sustainable management of these plant genetic resources. To this end, an ethnobotanical survey was carried out among 640 Senufo indigenous people, using a questionnaire. A total of 40 leafy vegetable species belonging to 28 genera and 20 families were identified. In fact, the most represented families are Solanaceae, Amaranthaceae and Asteraceae. The majority of these species are herbs (58%). Of these species, 25% are introduced (exotic); they are used as substitutes for wild leafy vegetables by the population in times of scarcity. On the other hand, introduced species contribute to the diversity of leafy vegetables used in the preparation of “TCHONRON” sauce. In addition, the leafy vegetables used to prepare this local product are dominated by Guinean-Congolese and Sudanese-Zambezian (GC-SZ) species, representing 55% of all species inventoried. This study reveals that the leafy vegetables used to prepare the “TCHONRON” sauce are diversified, and that the consumption of this sauce, a product of the Senoufo region, is not only cultural, but also contributes to the prevention of several diseases.

Physio-Morphological Traits Contributing to Genotypic Differences in Nitrogen Use Efficiency of Leafy Vegetable Species under Low N Stress

Soil fertility is declining in low-input agriculture due to insufficient fertilizer application by small-scale farmers. On the other hand, concerns are rising regarding the environmental pollution of both air and water in high-input agriculture due to the excessive use of N fertilizers in short growing seasons for vegetable crops, which is directly linked to the health of human beings and environmental safety. This study aimed to determine genotypic differences in the Nitrogen Use Efficiency (NUE) levels of different leafy vegetable species (Arugula, Spinach, Cress, Parsley, and Dill) grown hydroponically under two different N rates, low N (0.3 mM) and high N (3.0 mM), and to identify the plant traits that are contributing to NUE. A nutrient solution experiment was conducted between March and April 2024 by using an aerated Deep-Water Culture (DWC) technique in a fully automated climate room with a completely randomized block design (CRBD) with three replications for five weeks. The results indicated that shoot growth, as well as root morphological and leaf physiological responses, was significantly (p < 0.001) affected by genotype, the N rate, and genotype–N rate interactions. Shoot growth in some vegetable species (Arugula, Spinach, and Cress) was significantly higher under a low N than a high N rate, illustrating that they have a great capability for NUE under low N stress conditions. Similar results were also recorded for the root growth of the N-efficient species under low N rates. The NUE levels of these species were closely associated with leaf physiological (leaf area, leaf chlorophyll index (SPAD), photosynthesis, and total leaf chlorophyll (a + b) and carotenoids) and root morphological (root length, root volume, and average root diameter) characteristics. These plant traits could be useful indicators for the selection and breeding of ‘N-efficient’ leafy vegetable species for sustainable low-input agriculture systems in the future. However, further investigation should be carried out at the field level to confirm their commercial production viability.

Application of innovative biostimulants for growth and quality improvement in vegetable and ornamental crops

Research objective: This research aims to evaluate the biostimulant potential of some innovative products that can improve germination, growth and protection from biotic stresses on some vegetable and ornamental species. Materials and Methods: The experiments, which started in October 2022, were conducted in the CREA-OF greenhouses in Pescia (Pt), on Cichorium intybus and Crassula rupestris plants. The experimental groups were: i) group control, irrigated with water and previously fertilised substrate; ii) biofertiliser Ecklonia maxima, dilution 1:1000, 5 ml of this dilution once a week per plant; iii) Elixir lite, irrigated with water and substrate previously fertilised; iv) Immuno pro group, irrigated with water and substrate previously fertilised; Harvest boost group, irrigated with water and substrate previously fertilised. On 23 January 2024, plant height, number of leaves, vegetative weight, root volume and length, number of microorganisms and pH of the substrate were determined. In addition, the mortality of the plants in the nursery was assessed. Results and Discussion: The experiment showed that the use of biostimulants can significantly improve the vegetative and root growth of Cichorium intybus and Crassula rupestris plants. In general, a significant increase in plant height, vegetative and root weight and root length was observed, particularly in the Elixir lite treatment. There was also a significant increase in the microbial colonisation of the substrate with all innovative biostimulant treatments, while the pH remained practically unchanged. The Immuno pro treatment was the best in terms of reducing plant mortality in all two plant species. Biostimulants are widely used in horticulture because we often work with short-cycle crops, varying from a few weeks to a few months. The rapid succession of different crops implies a constant supply of nutrients and intensive use of soil. More intensive horticulture in particular often benefits from the effect of biostimulant treatments due to the increased resource use efficiency of the crops. Foliar biostimulants are mainly used by farmers to increase the production of both leaf and fruit vegetable crops. The positive effects of their use are exerted both on increasing the content of secondary metabolites and on a general improvement of nutrient uptake efficiency. These biological effects can be attributed to the presence in biostimulants of polysaccharides, extracted from algae, such as alginates and carrageenan, which are responsible for two important biological actions: sequestering or slowly releasing nutrients. In fruit vegetables, biostimulants can also improve the homogeneity of flowering and fruit size, as reported for peppers. Commercial biostimulants have increased the unit yield of many leafy vegetable species by acting on nutrient uptake by the plant. Conclusions: In horticultural production, in open field and in protected crops, the application of biostimulants is aimed at achieving one or more of the following objectives: to favour a rapid emergence of seedlings in direct sowing crops or a rapid overcoming of the transplanting crisis; to precociate the entry into production; to increase growth, flowering, fruit set and fruit growth; to improve product quality; to increase the efficiency of nutrient use and tolerance to environmental stresses.

A survey on the composition and occurrence frequency of arbuscular mycorrhizal fungi in rhizospheric soils and roots of vegetables grown in Ho Chi Minh city

The study aimed to determine the presence and composition of arbuscular mycorrhizal fungi (AMF) in the rhizospheric soils and roots of vegetable plants (14 species of leafy vegetables) grown in Hoc Mon, Cu Chi, and Binh Chanh districts, Ho Chi Minh city. The results of the isolation of AMF spores based on the wet sieving method showed the presence of AMF in the rhizospheric soils of vegetables with an average spore density of 87.6 spores/50 g of soil. For the symbiotic ratio of AMF to the root tissues, there was no symbiosis in 4 vegetable crops, including amaranth, mustard green, leaf mustard, and bok choy. Still, there was symbiosis in the remaining 10 plants, with an average symbiotic rate of 6.6%. Identification of AMF spores based on the morphological characteristics recorded in all the soil samples appeared spore phenotypes of 5 fungal genera including Glomus, Acaulospora, Gigaspora, Scutellospora, and Sclerocystis. However, 3 morphological types were not yet identified. From the obtained results, two genera, Glomus and Acaulospora, have the highest frequency at 43.9 and 39.6%, respectively.

Métodos de inoculação de Azospirillum brasilense em alface e rúcula em sistema hidropônico

ABSTRACT: Lettuce and arugula are the most cultivated leafy vegetables in hydroponic systems in the world, and the little information about Azospirillum brasilense effects in research in this system makes it necessary to verify the best method of inoculation of these bacteria. Therefore, this study aimed to verify the beneficial effects of inoculation methods of A. brasilense on plant growth and nutrition of lettuce and arugula grown in the hydroponic system. The study was conducted in a greenhouse with 30% shading. A randomized block design with five replicates in a 4 × 2 factorial scheme was used. Four A. brasilense inoculation methods (non-inoculated, foliar application, nutrient solution, and nutrient solution + foliar application) and two leafy vegetable species - arugula and lettuce - were evaluated. All inoculation methods improved plant height, shoot fresh and dry mass, root fresh and dry mass, fresh leaf yield, leaf chlorophyll index, accumulation of nitrogen, potassium, calcium, sulfur, phosphorus, iron, manganese, and copper and reduced nitrate accumulation in shoot and root of arugula and lettuce. Inoculation via nutrient solution + foliar application of A. brasilense is the most suitable for hydroponic arugula cultivation because it provides greater fresh leaf yield. All inoculation methods increased leaf yield in hydroponic lettuce. Hydroponic arugula and lettuce plants showed greater accumulation of nutrients and plant growth with less nitrate accumulation in the shoot under inoculation with A. brasilense.

Iodine biofortification of Swiss chard (Beta vulgaris ssp. vulgaris var. cicla) and its wild ancestor sea beet (Beta vulgaris ssp. maritima) grown hydroponically as baby leaves: effects on leaf production and quality.

About 35-45% of the global population is affected by iodine deficiency. Iodine intake can be increased through the consumption of biofortified vegetables. Given the increasing interest in wild edible species of new leafy vegetables due to their high nutritional content, this study aimed to evaluate the suitability of Swiss chard (Beta vulgaris ssp. vulgaris var. cicla) and its wild ancestor sea beet (Beta vulgaris ssp. maritima) to be fortified with iodine. Plants were cultivated hydroponically in a nutrient solution enriched with four different concentrations of iodine (0, 0.5, 1.0, and 1.5 mg L-1 ), and the production and quality of baby leaves were determined. Sea beet accumulated more iodine than Swiss chard. In both subspecies, increasing the iodine concentration in the nutrient solution improved leaf quality as a result of greater antioxidant capacity - the ferric reducing ability of plasma (FRAP) index increased by 17% and 28%, at 0.5 and 1.5 mg L-1 iodine, respectively - the content of flavonoids (+31 and + 26%, at 1 and 1.5 mg L-1 of iodine, respectively), and the lower content of nitrate (-38% at 1.5 mg L-1 of iodine) and oxalate (-36% at 0.5 mg L-1 of iodine). In sea beet, however, iodine levels in the nutrient solution higher than 0.5 mg L-1 reduced crop yield significantly. Both subspecies were found to be suitable for producing iodine-enriched baby leaves. The optimal iodine levels in the nutrient solution were 1.0 in Swiss chard and 0.5 mg L-1 in sea beet, as crop yield was not affected at these concentrations and leaves contained enough iodine to satisfy an adequate daily intake with a serving of 100 g. © 2023 Society of Chemical Industry.

Light spectrum effects on rocket and lamb's lettuce cultivated in a vertical indoor farming system

Rocket and lamb's lettuce are new leafy vegetables whose consumption in salads is increasing, especially as ready-to-eat products. These plants are often cultivated in greenhouses under artificial light, and are very important due to their pleasant taste, texture, and nutritional value to humans. The present study analyzes the effects of light quality provided by light emitting diodes (LEDs) on the yield and quality of different leafy vegetable species. Lamb's lettuce and rocket plants were grown in the same conditions under 4 light spectrums color fractions (NS-12, AP57, AP673L, and G2) for 27 and 37 days, respectively. Then fresh and dry weight, chlorophyll fluorescence, and chromatic characteristics were determined. The leaf quality of lamb's lettuce and rocket was characterized by measurements of the contents of carbohydrates, organic acids, and amino acids in leaves. Vegetative growth was significantly influenced by light quality and was species-dependent, and treatment with the highest proportion of Red:Blue light (G2) produced the highest fresh weight and maximum efficiency of PSII. The results of the chlorophyll fluorescence parameters were more influenced by the species rather than by the spectrum lights, except for the efficiency of PSII, which was higher in treatments with the lowest Red: Far-red ratio (AP67 and G2). G2 light spectrum reduces chlorophyll and carotenoid content compared to other treatments. Among all the primary metabolites analyzed, non-structural carbohydrates were the most dependent on light quality. Generally, the lights with the greatest effect on the parameters analyzed were those with higher Red:Blue ratios and a higher far-red fraction. No effect of light spectra on glutamate concentration was observed. However, a concentration increase was observed in treatments with a higher proportion of far-red light. Regarding organics acids, the different light spectrums did not affect the concentration of the majority of acids (citrate and malate) in rocket and lamb´s lettuce plants. The spectral composition of light has an influence on the quality and quantity of lamb's lettuce and rocket production. Therefore, each species studied responds differently to the light spectrums.

Levels of Some Metal Contents in the Soil and Leafy Vegetables Collected From Al-Qanat Highway/ Baghdad City

The aim of the present study is to estimate the levels of some essential and non-essential metals content of soil and seven leafy vegetable species, in the field 30m away from the Al-Qanat highway in Baghdad using X-ray fluorescence analysis. The results showed that soil and leafy vegetables had higher levels of macro elements, and that Ca in rocket and purslane was the highest. The average content of trace elements in soil and leafy vegetables has been close to the minimum than the range of permissible limits recommended by WHO and FAO/WHO except Fe, Cr and Ni in soil have exceeded the concentration of the threshold limit (13728.8 mg/kg Dw for Fe, 183.361mg/kg Dw for Cr, and 124.01mg/kg Dw for Ni) value as well for leafy vegetables, the concentration of Fe (573.29 mg/kg Dw in spinach and 540.41 mg/kg Dw in rocket) and zinc (183.52 mg/kg Dw in mallow, and 181.19 mg/kg Dw in purslane) exceeded the permissible limits. The values of As, Cd, Pb and Hg concentrations in soil have been within the range of permissible limits recommended by WHO while in leafy vegetables higher levels of Pb (2.58 mg/kg Dw), and Cd (2.2 mg/kg Dw), were found in the rocket, and fenugreek, respectively. Also, higher levels of Sr were found in the rocket (592.42 mg/kg Dw) and purslane (560.34 mg/kg Dw) as well as Al in spinach (186.55 mg/kg Dw). In addition, P, K, Mg (purslane), Zn (rocket, purslane, mallow), Cd (grapevine, fenugreek), and Sr (rocket, purslane) accumulated the most with the transfer factor (TF) (> 1.0), followed by Ca, Mg, Fe, Co, Cu, Se, Mn, Zn, Ni, As, Cd, Pb, Hg and Sr (0.01–1.0), while Cr, and Al had the lowest accumulation (< 0.01).

A comprehensive assessment of heavy metal(loid) contamination in leafy vegetables grown in two mining areas in Yunnan, China-a focus on bioaccumulation of cadmium in Malabar spinach.

Contamination of leafy vegetables grown in heavy metal(loid)-polluted mining areas pose serious health risks. This study aimed to explore the heavy metal(loid) contamination of leafy vegetables near two mining areas, by collecting samples from 14 different leafy vegetable species in Yunnan Province, China. The lead (Pb), cadmium (Cd), arsenic (As), and copper (Cu) contents of the samples were determined, and risks to human health were calculated using the hazard quotient and hazard index (HI). Moreover, Malabar spinach was identified as a leafy vegetable that exhibits low accumulation of heavy metal(loid)s. The accumulation capacity of different Malabar spinach varieties was verified, and a Cd soil safety threshold was determined using a pot experiment. Overall, Pb and Cd were the main soil and vegetable contaminants found in both study sites. The HI values for all leafy vegetables, apart from Malabar spinach, were greater than 1, indicating the presence of risks to human health; moreover, the health risks were greater for children than adults. The Malabar spinach pot experiment results showed that only some Cd forms exceeded China's maximum permissible standards. Furthermore, Malabar spinach varieties A (instant Malabar spinach), C (extra-large leaf green vine Malabar spinach), and F (large leaf Malabar spinach) displayed the lowest Cd accumulation. We calculated Cd total and bioavailable soil safety thresholds of 4.75 and 0.77 mg kg-1, respectively. However, further research is required to validate soil heavy metal safety thresholds for different vegetables. Ultimately, the heavy metal(loid) contamination of leafy vegetables described here was more serious than anticipated. Finally, the results of this study can inform residents living near these mining areas of a low-risk leafy vegetable, which will reduce the harm caused by heavy metal(loid) contamination in the area.

Contribution of Superabsorbent Polymers to Growth and Yield of African Leafy Vegetables

Superabsorbent polymers (SAPs) have been used as water-saving materials for limited irrigation water resources in arid and semi-arid areas. The purpose of this study was to optimize SAP application dosage and irrigation intervals on the growth of African leafy vegetables (ALVs) under greenhouse conditions. A factorial experiment was conducted under greenhouse conditions to determine the optimal SAP application, dosage, and irrigation intervals for the growth and production of African leafy vegetables. Two species of African leafy vegetables were studied: cowpea (Vigna unguiculata) and African nightshade (Solanum scabrum). The experiment was performed in a randomized complete block design comprising 25 treatments: a factorial combination of five superabsorbent polymer application doses (0 g, 0.5 g, 1 g, 1.5 g, and 2 g) SAP/Kg of soil substrate and five irrigation intervals (3 days, 4 days, 5 days, 6 days, and 7 days) with three replications. Statistical analysis of the results showed that the use of SAP in irrigation can significantly promote the growth and yield of African leafy vegetables. SAP, irrigation, and their interactions significantly affected ( P < 0.005 ) the plant height, stem diameter, leaf area, number of mature leaves/plant, and dry weight of leafy vegetables. Treatment with SAP showed higher growth parameters and yield of vegetables compared to the treatments. Treatment with 1 g SAP/kg of soil substrate and 5-day irrigation interval recorded the highest plant growth and yield, with 24%, 11.7%, 11.1%, and 85.9% increase in cowpea plant height, leaf area, number of mature leaves, and dry weight/plant, respectively. The treatment with 1 g SAP/kg of soil substrate and 5-day irrigation interval was observed as the best for the optimal production of African leafy vegetables under greenhouse conditions. Hence, SAP could be a good strategy for food production within arid and semi-arid lands where water resources are scarce.

Agronomic, Genetic and Quantitative Trait Characterization of Nightshade Accessions.

Nightshades are among many underutilized and neglected African indigenous leafy vegetable (AILVs) species, and if adequately exploited, they could improve food, nutrition and income among the rural population. Morphological characterization of available accessions is key for the breeder to identify and select superior accessions as parents for utilization in breeding programs. Fifteen accessions of nightshade were evaluated for morpho-agronomic variation in an open field trial implemented in a randomized complete block design with three replicates across the two growing seasons. The accessions exhibited significant (p < 0.0001) differences in all quantitative traits. The data analysis showed that Scabrum (805.30 g/plant) followed by Ncampus (718.60 g/plant) produced the highest fresh leaf yield; for fruit fresh yield, the accession NigSN18 (1782.00 g/plant) recorded the highest, followed by ManTown (1507.90 g/plant). The accession N5547 had the tallest plants (66.83 cm), followed by accession Timbali (62.31 cm). The first four principal components (PCs) accounted for 86.82% of the total variation, which had an eigenvalue greater than 1. The cluster analysis grouped the accessions into 14 clusters based on their genetic similarity. Results of genetic studies revealed that phenotypic coefficient variation was higher than genotypic coefficient of variation for all parameters evaluated, indicating the environmental influence on the expression of these traits. Both GCV and PCV were higher for the largest leaf area, moderate to high for the remaining characters and low for leaf fresh yield per plant. High heritability coupled with genetic advance as a mean percentage (H2-70.59%, GAM-142.4%), indicating the presence of additive gene effects. Hence, selection can be employed for the improvement of this trait in nightshades. The study revealed sufficient genetic variability in the nightshade accessions, which can be exploited for crop improvement.

Phytonutrients, Colorant Pigments, Phytochemicals, and Antioxidant Potential of Orphan Leafy Amaranthus Species.

The underutilized Amaranthus leafy vegetables are a unique basis of pigments such as β-cyanins, β-xanthins, and betalains with radical scavenging capacity (RSC). They have abundant phytonutrients and antioxidant components, such as pigments, vitamins, phenolics, and flavonoids. Eight selected genotypes (four genotypes from each species) of underutilized Amaranthus leafy vegetables were evaluated for phytonutrients, pigments, vitamins, phenolics, flavonoids, and antioxidants in a randomized complete block design under ambient field conditions with three replicates. The studied traits showed a wide range of variations across eight genotypes of two species of Amaranthus leafy vegetables. The highest fat, β-xanthins, K, dietary fiber, Mg, β-cyanins, Mn, chlorophyll ab, Zn, TP, TF, betalains, chlorophyll a content, and (RSC) (DPPH) and RSC (ABTS+) were obtained from A. tricolor accessions. Conversely, the highest protein, Cu, carbohydrates, Ca, and chlorophyll b content were obtained from A. lividus accessions. The highest dry matter, carotenoids, Fe, energy, and ash were obtained from A. tricolor and A. lividus. The accession AT2 confirmed the highest vit. C and RSC (DPPH) and RSC (ABTS+); AT5 had the highest TP content; and AT12 had the highest TF content. A. tricolor accessions had high phytochemicals across the two species, such as phytopigments, vitamins, phenolics, antioxidants, and flavonoids, with considerable nutrients and protein. Hence, A. tricolor accessions can be used as high-yielding cultivars comprising ample antioxidants. The correlation study revealed that vitamin C, pigments, flavonoids, β-carotene, and phenolics demonstrated a strong RSC, and showed a substantial contribution to the antioxidant potential (AP) of A. tricolor. The investigation exposed that the accessions displayed a plentiful origin of nutritional values, phytochemicals, and AP with good quenching ability of reactive oxygen species (ROS) that provide enormous prospects for nourishing the mineral-, antioxidant-, and vitamin-threatened community.

Assessing the utility of selected photosynthetic and related traits in screening Amaranthus dubius Mart. ex Thell. and Galinsoga parviflora Cav. 1796 seedlings for elevated temperature stress tolerance

Wild leafy vegetables are of increasing interest because many have higher concentrations of some beneficial minerals, vitamins and nutrients than commercial crops. Some reports also indicate that they exhibit high levels of tolerance to abiotic stressors associated with climate change. The aim of this study was to investigate whether selected biochemical, physiological (related to photosynthesis) and morphological traits could be used to screen for elevated temperature tolerance in two wild leafy vegetable species, viz. Amaranthus dubius (C4) and Galinsoga parviflora (C3). One-month-old seedlings were transferred from a greenhouse to growth chambers and subjected to either ambient [25°C (day)/14°C (night)] or elevated [30°C (day)/19°C (night)] temperatures for 15 days. On days 0, 5, 10 and 15, gas exchange, chlorophyll fluorescence, selected biochemical, physiological and morphological traits were determined. Data for the 24 traits measured were used in plant trait network analyses to establish the populations’ phenotypic plasticity, including changes in the interactions/relationships amongst the traits when exposed to elevated temperatures. Sixteen traits showed significant differences between ambient and elevated temperatures on day 15, 11 in G. parviflora only, one in A. dubius only and four in both species. Those shared by the two species were intracellular hydrogen peroxide, electrolyte leakage, ascorbate peroxidase, and superoxide dismutase, while specific leaf area was significantly different in A. dubius only. Both species altered biochemical leaf traits under elevated temperatures, while morphological leaf traits were altered in A. dubius only and photosynthetic leaf traits in G. parviflora only. In G. parviflora, the photosynthetic traits were more sensitive to elevated temperatures than in A. dubius. This suggests that photosynthetic traits may be ineffective for screening C4 species, due to them being highly evolved to survive in warmer climates. However, the results showed that photosynthetic traits could be useful as screening tools for C3 photosynthetic pathway species. The plant trait network analyses showed that both species displayed high phenotypic plasticity with a 5°C increase in temperature. We recommend when screening wild leafy vegetables for high temperature tolerance that photosynthetic traits be considered in the context of their phenotypic plasticity. This approach could fast-track the identification of more climate-resilient wild edible species.

Ethnobotanical survey of indigenous leafy vegetables in Ehlanzeni District of the Mpumalanga Province, South Africa

Background: There is a high loss of indigenous knowledge, resulting in negative effects on the health and lives of cultural people living in poor communities mostly in the rural areas, hence, an urgent need for indigenous knowledge conservation. Aim: This study is aimed at presenting the potentials of leafy indigenous vegetables as an essential source of food and nutrition for poor communities. We argue that through knowledge transfer, these species have a chance of being revitalised and used, thereby conserving plant biodiversity while ensuring food and nutrition security. Setting: An ethnobotanical survey was conducted in the Ehlanzeni District of the Mpumalanga Province, South Africa, to uncover and document the indigenous leafy vegetables (ILVs) from the area as well as to evaluate the community’s indigenous vegetable knowledge and utilisation state. Methods: Using a structured questionnaire, 95 respondents were interviewed. Older women were particularly targeted since they are the repositories of ethnobotanical information related to ILVs. Results: The study revealed 17 indigenous leafy vegetable species from 10 families. About 85% of the ILVs cited possessed medicinal properties. The most common means of preservation was sun-drying, although consumption of vegetables in their fresh state was most preferred. Conclusion: High blood pressure was cited to be treated by most of the ILVs; therefore, there is a need to include these vegetables in our daily diets.

Vocal to local: indigenous dietary practices and diversity of wild food plants in Malai Mahadeswara wildlife sanctuary, South India

Background: The voice of the indigenous food system on locally available wild food plants and consumption is an important strategy to sustain interrelated food problems of malnutrition and disease. Methods: The study assessed the importance of wild food plant use among the forest-dwelling communities. Community perceptions were used to assess the patterns of use and interrelations of human well-being. Data was collected through a combination of semi-structured interviews, household surveys, and focus group discussions in eight villages. Local communities use wild plant species as food, therapeutic practices, and as a symbol of ethnic identity. Results: The taxonomical distribution and diversity of 126 species belonging to 94 genera and 58 families have been assessed. About 83% of wild leaves as greens fall in the category of weeds. There were 15 species WFPs that have been shared with neighbours in the village, close relatives, or friends. More than 28 species of leafy vegetables are used by 80% of households for more than 20 days a year. The local communities also use 120 wild edible herbs and root species in ethno-medicine. Boerhavia diffusa, Acacia farnesiana, and Alternanthera sissilis have been used frequently as vegetables, they were reported to reduce blood pressure, increase iron in the blood, and improve eyesight. Conclusions: The study emphasizes the dependency on the local food source and its livelihood importance. The study would help to evaluate the potential of WFPs use as future food in indigenous dietary systems and therapeutic practices. Keywords: Indigenous food system, dietary diversity, therapeutic value, wild food plants

Antidiabetic green leafy vegetables currently sold in Trincomalee District in Sri Lanka

Diabetes is a disease in which the body does not produce insulin or cannot use insulin effectively. In Sri Lanka, 1.2 million people among the age group of 20 to 79 were affected by diabetes in 2019. Biomedicine antidiabetic medications cause common side effects such as bladder cancer, dehydration, sinusitis, kidney illness, and urinary infections. Consumption of green leafy vegetables minimizes the risk of developing diabetes. Hence, this work aims to identify and document the green leafy vegetables currently sold in Trincomalee District in Sri Lanka by performing fieldwork visits to the main markets and the surrounding green leafy vegetable kiosks, shops, superstores, and streets from January 2019 to June 2021. The levels of antidiabetic scientific evidence available for the identified green leafy vegetable species were assessed by recognizing the relevant published works in the electronic databases Web of Science, PubMed, Scopus, etc., until September 2021. A total of 99 green leafy vegetable species from 43 families were recognized. The majority of the green leafy vegetables had in vivo (33%), followed by in vitro (16%) and clinical (9%) as scientific evidences. Also, 49 antidiabetic compounds were already isolated from the identified green leafy vegetables. This study forms a foundation for further studies using the green leafy vegetable species in Trincomalee.

Documentation of Wild Edible Leafy Vegetable Traditionally Used by Tribal and Rural Communities of North Maharashtra, India

Number of wild edible plants is commonly used in the traditional diets of tribal people in many parts of the world. North Maharashtra is well known for its tribal region and tribes from this region partially or fully dependent on the wild resources for their nutritional requirements. The present study was designed to document specifically the wild leafy vegetables from North Maharashtra. A total of 62 traditionally used wild leafy vegetable species were collected, identified and documented. Out of 62 species, 61 species belong to Angiosperms and 1 belong to pteridophyte. With respect to families Amaranthaceae, Araceae, Asteraceae and Fabaceae were found to be the largest families with 29 species. Herbs are the major source of wild leafy vegetables with 43 species and forest is the home for the majority of wild leafy vegetables. Due to less awareness, loss of vegetation and fast erosion of traditional knowledge many species are on the line of rarity. The study helps to conserve those wild food species and cultivate them on large scales, to uplift their economic status and sustainable management in near future.

The Ethnobiology of Contemporary British Foragers: Foods They Teach, Their Sources of Inspiration and Impact

Foraging in the British Isles is an increasingly popular activity for both personal consumption and for commercial purposes. While legislation and guidelines exist regulating the sustainable collection of wild edibles, the founding principles of the British foraging movement are not well documented. For this research, 36 of the most active foraging instructors of the Association of Foragers were interviewed to understand their background, species collected, sources of knowledge, and problems faced during collection. Altogether, 102 species of leafy vegetables, fruits, fungi, and seaweeds were mentioned as frequently used, while 34 species of roadkill animals were listed, mostly for personal consumption. Instructors reported learning from wild food guidebooks, other foragers, or personal experience. Frequent contact among foragers has led to the standardisation of knowledge and practices among them forming a “new tradition”, partly based on old British traditions but modified by influences from other countries and cultures, both in terms of choice of species and processing techniques. Contrary to expectations, foragers rarely reported clashes with nature conservation or forestry managers. The authors argue that knowledge and practice developed by the Association of Foragers (AoF) are sustainable and could be integrated into the British food and nature conservation system.

Nutritive composition and essential elements evaluation of some leafy vegetables grown in Gubi Fadama Land, Bauchi, Nigeria

Proximate analysis and some essential elements content of four leafy vegetable species namely: Brassica oleracea, Spinacia oleracea, Lactuca sativa and Hibiscus sabdariffa leaves were determined using standard analytical methods. These vegetables were widely consumed as food across Northeastern Nigeria. It is imperative to ascertain their nutritive values as they now form part of human diet for people in the area. Some of the results of the mean values ranged such as ash (2.33±0.75–6.03±1.23%), moisture (23.60±0.76–30.90±0.34%), lipid (0.45±0.00– 4.73±0.21%) and protein (2.62±0.10–3.38±0.013%). The mineral elements values ranged like potassium (145.47±4.50– 35.60±2.45mg/100g), sodium (74.97± 0.20– 389.85 ±0.02 mg/100g), calcium (9.87±0.13 –14.55 ±0.94mg/100g) iron (1.30±0.05–2.17±0.15 mg/100g), magnesium (0.030±0.00–0.047± 0.01mg/100g and manganese (0.004±0.00–0.015±0.00 mg/100g). The work suggested that the leaves investigated contained appreciable amounts of nutrients. Their consumption could augment the unavailable or rarely available nutrients in human body. Comparatively, they are good sources of K, Na, Ca and Fe, as well as fiber, moisture, and carbohydrates on the dietary menu recommendation. All the studied leaves have low lipid and protein content as well as copper, magnesium, and manganese.

Growth and vegetable yield ofAmaranthus hybridusL. as impacted by harvest methods in southern Ontario, Canada

Amaranthus hybridus L. is a nutritious leafy vegetable amaranth species grown primarily in tropical regions. Weedy amaranth species thriving in southern Ontario suggest the edible biotype could also grow efficiently as a crop. Field trials in Guelph, Simcoe, and Stouffville, Ontario, examined yield in response to three harvest frequencies (weekly, every 2 wk, and every 3 wk) cut at 15 cm above ground level, using two traditional harvest practices (Afro-Caribbean and European) cut every 2 wk, and a control which was not cut until the final harvest. The highest marketable yields and quality measures were observed in plants cut every 2 wk, every 3 wk, and using the Afro-Caribbean cutting technique which selects and harvests thicker stems while leaving new shoots for later harvests. The difference in growth among sites suggests plants prefer warm, well-drained soil. Marketable yield was as high as 4.02 ± 0.340 kg m−2in Simcoe with an average yield across all three sites of 2.37 ± 0.229 kg m−2. This demonstrates that A. hybridus has the potential to be grown as a vegetable crop in southern Ontario and that marketable yield can be optimized by method of harvest.