Cicadellidae Species Research Articles

The Complete Mitochondrial Genomes of Five Nivanini Species (Hemiptera: Cicadellidae: Evacanthinae) With Phylogenetic Analysis.

To delve deeper into the phylogenetic relationships within Cicadellidae and the taxonomic arrangement of Evacanthinae, our study focuses on the mitochondrial genome sequencing of five Nivanini species: Extensus latus, Concavepiana hamulusa, Sophonia nigrilineata, Sophonia microstaina, and Sophonia fuscomarginata. The results showed that the length of the five mitochondrial genomes ranged from 15,610 to 16,032 bp and included 37 typical genes. The A + T content of Nivanini ranged from 72.5% to 78.7%, which is consistent with that of other sequenced Evacanthinae species. All transfer RNAs (tRNAs) exhibit the typical cloverleaf secondary structure, except for trnS (AGY), which lacks the dihydrouracil (DHU) arm. With regard to protein-coding genes, all started with ATN codons, except for atp8, and most of them use TAA or TAG as termination codons. Using the Bayesian inference and maximum likelihood methods, a phylogenetic tree based on all 37 genes was constructed, with a total of 57 Cicadellidae species and two outgroups included as research objects. The analyses confirmed the monophyletic nature of each subfamily, highlighting Deltocephalinae as the oldest, distinctively parallel to the others.

Population Dynamics of Potential Insect Vectors of Xylella fastidiosa (Xanthomanadales: Xanthomonadaceae) and Other Auchenorrhyncha in Olive and Citrus Groves of Crete, Greece

This study investigated the phenology and population dynamics of potential insect vectors of Xylella fastidiosa Wells et al. and other Auchenorrhyncha species in olive and citrus groves of Chania province, Crete, Greece. Although X. fastidiosa has not been reported in Greece, its introduction could cause serious diseases in many crops, including olives and citrus. Olive groves of Olea europaea L. ‘Koroneiki’ were sampled systematically using sweep net and Malaise traps over 24 months. One citrus grove was sampled for one year using a Malaise trap. Sweep net samples were taken from the herbaceous cover, tree canopy, and field borders of olive groves. Auchenorrhyncha were more abundant on the herbaceous cover compared to the canopy and field margins. Aphrophoridae species were mostly found on the herbaceous cover and in low numbers during fall (October–December) and spring (April–May). Cicadellidae species, such as Euscelis spp., were frequently found on the herbaceous cover of both olive and citrus groves. One Aphrophoridae and several Cicadellidae species were recorded in the citrus grove. Altitude was found to influence the population abundance of some Auchenorrhyncha species in olive groves. These results provide information for effective integrated management of insect vectors and their vector-borne pathogens.

Inventory of leafhoppers in irrigated rice in Tamil Nadu

Leafhoppers diversity was studied in irrigated rice ecosystems of Cauvery Delta Zone, High Rainfall Zone and Western Zone. Leafhopper assemblage comprised 14 species of Cicadellidae and among them Maiestas dorsalis (Motschulsky) was found to be the most abundant species (35.02%), followed by Cofana spectra (Distant) (23.15%). The order of species diversity in the observed districts were Western Zone (13 species) > High Rainfall Zone (8 species) > Cauvery Delta Zone (4 species). Shannon Weiner Index revealed a higher diversity in High Rainfall Zone and Western Zone (H'= 1.77 > 1.76). Margalef's Index of species richness was observed highest in Western Zone (a = 1.93). Pielou's Evenness Index was found to be almost similar for Western Zone (0.30) and Cauvery Delta Zone (0.29) while High Rainfall Zone exhibited highest evenness (0.37). Jaccard index of similarity of 50% (highest) was observed between Western Zone and Kanyakumari, Cauvery Delta Zone and High Rainfall Zone. Berger-Parker Index of dominance (D) revealed M. dorsalis to be the most dominant in Western Zone (0.25) and Cauvery Delta Zone (0.66) while High Rainfall Zone showed a high dominance of Nephotettix nigropictus (Stål) (0.27). Among the leafhoppers studied Balclutha incisa (Matsumura), Cicadulina bipunctata (Melichar), Empoascanara maculifrons (Motschulsky), Exitianus sp., Thomsonia porrecta (Walker) were recorded for the first time in rice ecosystem of Tamil Nadu. The present study will be a baseline information on the diversity of leafhoppers in irrigated rice of Tamil Nadu and will complement the data collected in the future to gain a holistic understanding on the leafhopper diversity of Tamil Nadu.

Nesting behaviour and description of the larva of Alysson spinosus (Panzer, 1801) (Hymenoptera: Bembicidae)

The paper presents new data on the nesting habits of the digger wasp Alysson spinosus (Hymenoptera: Bembicidae). As food for larvae, the female provisioned about 7–8 hemipteran nymphs or imagines per cell. The collected prey belongs to two families of true hoppers (five species of Delphacidae and one species of Cicadellidae). Nests are built in sandy, shaded areas, and consist of a 10–12 cm long main burrow with 1–3 brood cells. Both sexes search for food (honeydew) on the leaves of lilac or small-leaved linden. The nests were infested by the dipteran kleptoparasitic Metopia argyrocephala (Diptera: Sarcophagidae). The mature larva is similar to that of the Nearctic Alysson melleus, from which it differs in having a blunt apical mandibular tooth and more prominent setae on the clypeus and labrum.

Epidemiological characterization of a disease associated with phytoplasmas in Andean oak, Quercus humboldtii Bonpland, in Bogotá—Colombia

AbstractQuercus humboldtii is the dominant tree species in oak forest ecosystems in the Colombian Andes, and approximately 15,000 trees have been planted in urban Bogotá. In Bogotá, Q. humboldtii is affected by phytoplasmas. The management of phytoplasmas relies mainly on control of the insect vectors. The aim of this work was to generate information on phytoplasmas affecting Q. humboldtii and the potential insect vectors involved. Phytoplasmas were detected by nested PCR and identified by restriction fragment length polymorphism or sequencing analysis of the 16S rRNA gene in 238 randomly selected urban trees, from 17 city zones. The prevalence of the disease was 80%, with phytoplasmas detected in 94% of trees. ‘Candidatus Phytoplasma fraxini’ was found in 54.6%, ‘Candidatus Phytoplasma asteris’ in 30.7% and mixed infections of both phytoplasmas in 8.8% of the trees. Phytoplasmas were observed in the phloem of infected trees by electron microscopy. ‘Ca. P. fraxini’ was more common than ‘Ca. P. asteris’ in both street and parks trees, but correlations between the phytoplasma and disease‐associated variables were not observed. The entomofauna of a subsample of 102 of these trees was examined. Representative specimens of seven orders were collected, with Hemiptera the most prevalent. Specimens of 17 Cicadellidae, one Membracidae and one Psyllidae species were collected. Cicadellidae species from subfamilies Aphrodinae, Deltocephalinae, Iassinae, Eurymelinae, Megophthalminae and Typhlocybinae were found, in which phytoplasma vectors have been reported previously. Specimens of two known Cicadellidae vector species were collected: Amplicephalus funzaensis (Deltochephalinae) and Exitianus atratus (Deltochephalinae). Phytoplasmas were assessed in 103 Cicadellidae specimens. ‘Candidatus P. asteris’ was detected in A. funzaensis (n = 3/16 tested), E. atratus (n = 2/10 tested) and Alebrini sp. 1 (n = 1/11) tested). The possible epidemiological implications of these findings are discussed.

Characterization of the leafhopper mitogenome of Mileewa alara (Hemiptera: Cicadellidae: Mileewinae) and its phylogenetic analysis

The mitogenome data of leafhopper species Mileewa alara was assembled and annotated in this study. The results shows that length of M. alara is 16020 bp, consist of 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and one control region. The A + T content in the mitogenome was 77.9%. Phylogenetic analysis based on 13 PCGs of four Mileewa species and other 29 Cicadellidae species, each subfamily species well separated. And M. alara clustered with M. ponta. This study also raised mitogenome of Mileewa number in GenBank to four.

Leafhopper food plants in a Neotropical forest in Panama (Hemiptera: Cicadellidae).

The leafhoppers (Cicadellidae) are a group of insects that suck sap from vascular plants. The host plants of most species of Cicadellidae are unknown, but some species are known to have a wide range of food plants, while others are host plant specific. The aim of this study was to record the food and host plants for leafhoppers in a Neotropical rainforest. The study area was located in the lowland forests of Panama Province (Central Panama) in two National Parks. Sampling was done in the undergrowth, canopy and emergent strata to collect the cicadellids feeding on the trees, shrubs, seedlings, and vines there. We collected 118 cicadellid adult representing 24 species, 21 genera, and six subfamilies, which fed on 49 species of plants, belonging to 31 families. The subfamily with the largest number of species was the Cicadellinae with 11, while the plant family with the most species was the Fabaceae with five species, and the favorite plant was Anacardium excelsum, which hosted six cicadellid species and 10 individuals. According to estimates by Shannon Weiner, leafhopper communities are more diverse on trees than on shrubs, vines, or grasses.

Cicadellinae of Ecuador and Cicadellidae of Galápagos

ABSTRACT An annotated list of the Cicadellinae (Hemiptera: Auchenorrhyncha: Cicadellidae) of Ecuador is presented. The collection in the Museum of Zoology Invertebrates Section of the Pontifical Catholic University of Ecuador (QCAZ) is comprised of 3763 specimens of Cicadellidae of which 2806 were in the subfamily Cicadellinae. These specimens were identified as belonging to 87 species. The collection contains an additional 28 species that appear to represent undescribed species. The specimens were distributed among all provinces of Ecuador except the new, small province of Santa Elena on the coast. The specimens were from every broad geographic region of Ecuador: Coast, Western Cloud Forest, Highlands, Eastern Cloud Forest and Amazonia. Many species were collected in multiple geographic regions. There are 18 new country records in this collection. The literature was searched for additional species of Cicadellinae and an additional 166 species were identified as part of the Ecuadorian fauna for a total of 253 species. Twenty-four species have host records that indicate they have the potential to be pests of cultivated and orchard crops. Twenty-four species of Cicadellidae in 16 genera are listed for the Galápagos Islands and many are considered endemic.

Characterization and phylogenetic analysis of the mitochondrial genome of Mileewa ponta (Hemiptera: Cicadellidae: Mileewinae)

The mitogenome of one Mileewinae species Mileewa ponta was sequenced and annotated according to close Cicadellidae species in this study. Annotation shows the mitogenome of M. ponta is 15999 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The A + T content in the mitogenome was 79.9%. Besides, we further constructed a Bayesian Inference phylogenetic tree among M. ponta, M. albovittata, M. margheritae and other Cicadellidae species. Each subfamily species clustered together, and M. ponta clustered with M. albovittata, M. margheritae. This mitogenome will contribute to the further systematics and evolution study of subfamily Mileewinae.

The mitogenome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae)

In this study, the mitochondrial genome of Mileewa margheritae (Hemiptera: Cicadellidae: Mileewinae) was sequenced and annotated. The mitogenome of M. margheritae is 15376 bp in length, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The A + T content in the mitogenome was 79%. Most of PCGs started with ATN and stopped with TAA while ATP8 and ND5 started with TTG, COX2, ND1 stopped with incomplete T, Cytb stopped with TAG. We further constructed a Bayesian phylogenetic tree among M. margheritae, M. albovittata and other Cicadellidae species. The constructed phylogenetic tree suggests the close evolutionary relationship between M. margheritae and M. albovittata.

Characterization of the complete mitochondrial genome of Mitjaevia protuberanta（Hemiptera: Cicadellidae: Typhlocybinae）

The complete mitogenome of Mitjaevia protuberanta (GenBank accession number MN627216) is 14,032 bp (AT: 77.43%) in length, including 13 protein-coding, 22 transfer RNAs, and 2 ribosomal RNAs. All protein-coding genes used ATN as initiation codon except ND5 that used TTG as initiation codon, and TAA, TAG, and T were termination codons. We constructed a phylogenetic tree from 14 species (Hemiptera) based on the nucleotide sequence of 13 mitochondrial protein-coding genes. The phylogenetic analysis results showed that mitochondrial genome of M. protuberanta had the same characteristics as other Cicadellidae species.

The species of Cicadellidae, Cixiidae and Delphacidae (Hemiptera) on Karacadag rice and the effects of weeds on their populations

Karacadag celtiginde Cicadellidae, Cixiidae ve Delphacidae (Hemiptera) turleri ile yabanciotlarin bunlarin populasyonlarina olan etkileri, 2010-2012 yillari arasinda Diyarbakir ve Sanliurfa illerinde arastirilmistir. Turleri belirlemek icin orneklemelerde atrap ve D-vac. kullanilmis ve Cicadellidae ve Delphacidae familyasinin yabanciotlu ve yabanci otsuz parsellerdeki populasyon yogunluk ve degisimleri icin tesaduf bloklari deneme desenine gore sari yapiskan renk tuzaklar asilarak izlenmistir. Calisma ile Cicadellidae familyasindan 10, Cixiidae familtyasindan 3 ve Delphacidae familyasindan 4 tur olmak uzere toplam 17 tur belirlenmistir. Cicadellidlerden en yaygin ve yogun turlerin Agallia harrarensis Melichar, 1911 ile Empoasca sp., Cixiidlerden Tachycixius desertorum (Fieber, 1876) ve Hyalesthes obsoletus Signoret 1865, Delphacidlerden ise Laodelphax striatellus (Fallen), Sogotella vibix Mats oldugu tesbit edilmistir. Celtikteki yabanciotlarin cicadellid populasyonunu artirdigi belirlenmis, bireylerin %71.4’u yabanciotlu alandan, %28.6’si ise yabanciotsuz alandan elde edilmistir. Delphacid ve cixiid populasyonunun ise yabanciot bulunan parsellerde populasyon az miktarda yuksek bulunmustur. Cicadellidae ve Delphacidae populasyonun, celtigin vejetatif doneminde baslayarak artmaya basladigi generatif-olgunlasma doneminde en ust seviyeye ulastigi belirlenmistir. Sonuc olarak yabanciot mucadelesinin celtik alanlarinda onemle yapilmasi gerektigi dusunulmektedir.

Responses of community-level plant-insect interactions to climate warming in a meadow steppe.

Climate warming may disrupt trophic interactions, consequently influencing ecosystem functioning. Most studies have concentrated on the temperature-effects on plant-insect interactions at individual and population levels, with a particular emphasis on changes in phenology and distribution. Nevertheless, the available evidence from the community level is limited. A 3-year field manipulative experiment was performed to test potential responses of plant and insect communities, and plant-insect interactions, to elevated temperature in a meadow steppe. Warming increased the biomass of plant community and forbs, and decreased grass biomass, indicating a shift from grass-dominant to grass-forb mixed plant community. Reduced abundance of the insect community under warming, particularly the herbivorous insects, was attributed to lower abundance of Euchorthippus unicolor and a Cicadellidae species resulting from lower food availability and higher defensive herbivory. Lower herbivore abundance caused lower predator species richness because of reduced prey resources and contributed to an overall decrease in insect species richness. Interestingly, warming enhanced the positive relationship between insect and plant species richness, implying that the strength of the plant-insect interactions was altered by warming. Our results suggest that alterations to plant-insect interactions at a community level under climate warming in grasslands may be more important and complex than previously thought.

Complete mitochondrial genome of Drabescoides nuchalis (Hemiptera: Cicadellidae)

The complete mitochondrial genome (mitogenome) of Drabescoides nuchalis (Hemiptera: Cicadellidae) was sequenced. It is 15 309 bp in length with 75.62% (A + T) content and comprises 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNA genes, and a non-coding region (GenBank accession no. KR349344). Gene order is identical to that of the inferred ancestral insect genome. All PCGs start with an ATN codon and terminate with TAA except ND4, which has an incomplete stop codon (T). The anticodons are identical to those of Drosophila yakuba. The phylogenetic tree confirms D. nuchalis and two Cicadellidae species are clustered into a clade, and Cicadellidae is a monophyletic group and provides support for the sister relationship of leafhopper and treehopper.

Abundance and Species Richness of Leafhoppers and Planthoppers (Hemiptera: Cicadellidae and Delphacidae) in Brazilian Maize Crops

Insects in the Cicadellidae and Delphacidae families, common in grasses, are an important group of vectors of viruses and mollicutes, which cause diseases in several plant species. The goal of this study was to evaluate the abundance and species richness of Cicadellidae and Delphacidae and the presence of potential vectors of viruses and mollicutes in maize crops in Brazil. Insects were collected using sweep nets in maize crops in 48 counties of 8 states, distributed in 4 regions of Brazil in the yr 2005, 2006 and 2007, with a total of 198 samples. The collected material was screened, and the leafhoppers and planthoppers were identified at the species level. A total of 4233 Cicadellidae specimens, including 30 species, and 205 Delphacidae specimens, including 9 species, were collected. The most abundant species was Dalbulus maidis (Delong & Wolcott) representing 90.1% of leafhoppers and planthoppers. Abundance and species richness differences were observed between the northeast and center-south regions of Brazil. Eight species of leafhoppers and planthoppers were identified as vectors or potential vectors of viruses and mollicutes in maize, although some of these viruses are not reported in Brazil. Among these species, we identified the planthoppers, Caenodelphax teapae (Fowler), Peregrinus maidis Ashmed, Pyrophagus tigrinus Remes Lenicov & Varela and Toya propinqua (Fieber) are experimental vectors of Mal de Río Cuarto virus, which is a quarantine virus in Brazil. Two Cicadellidae species and 3 Delphacidae species were reported for the first time in Brazil.

Determination and population fluctuations of Cicadellidae (Hemiptera: Cicadomorpha) species in pomegranate orchards in Aydin Province, Turkey

Many Cicadellidae (Hemiptera: Cicadomorpha) species may reach high populations in fruit orchards. Cicadellids can cause damage not only by sucking plant sap, but also by transmitting detrimental plant pathogens, which may become more important in many cases. It was aimed to determine Cicadellidae species and population fluctuations of common species in pomegranate orchards in Aydin (Nazilli, Karacasu) province in Turkey. Studies were conducted in two pomegranate orchards through 2009-2010. Samplings were done with two yellow sticky traps and by sweep net with 100-sweep-collection on weeds in each orchard. Additionally, cicadellids were collected by shaking one branch of each tree and 20 trees in total in each orchard. Cicadellidae species and their populations were therefore investigated both on trees and weeds in orchards. As a result, 19 cicadellid species were found in orchards. Of these species, Docotettix cornutus Ribaut, Fieberiella anategea Meyer-Arndt, Asymmetrasca decedens (Paoli)+Empoasca decipiens Paoli and Zyginidia pullula (Boheman) were common on the trees and A. decedens (Paoli)+E. decipiens Paoli, Cicadulina bipunctella (Matsumura), Macrosteles quadripunctulatus (Kirschbaum) were common on the weeds in the orchards. Fieberiella anategea and D. cornutus populations reached high levels in JulyNovember on yellow sticky traps in 2009, but remain low during 2010. Asymmetresca decedens + E. decipiens populations reached high levels in May-June and October in 2009 and 2010. On the other hand, Z. pullula population stayed at a very low level in the years 2009 and 2010.

Leafhoppers and cixiids in phytoplasma-infected carrot fields: Species composition and potential phytoplasma vectors

The first molecular analysis of samples collected in southern Backa (Serbia) confirmed the presence of aster yellows (16SrI) and stolbur phytoplasmas (16SrXII) in insects belonging to the family Cicadellidae, as well as in carrot plants where the insects were collected. A correct identification of the phytoplasmas and their vectors is essential to arrange effective control strategies to prevent diseases associated with phytoplasmas from spreading to carrots and other vegetable crops. In order to enhance knowledge about insect vectors of aster yellows and stolbur phytoplasmas in Serbia, Cicadellidae and Cixiidae (Homoptera Auchenorrhyncha), the most common vectors of these phytoplasmas, were monitored in southern Backa during 2008. Adults leaf- and planthoppers were collected and identified at species level using standard entomological methods, and tested for phytoplasma presence by means of PCR/RFLP. A total of 13 insect species of Cicadellidae were identified, as follows: a) three species of the subfamily Agallinae: Anaceratagallia ribauti (Ossiannilsson), Anaceratagallia venosa (Fourcroy), and Anaceratagallia laevis (Ribaut); b) seven species of the subfamily Deltocephalinae: Psammotettix confinis (Dahlbom), Psammotettix striatus (Linnaues) Psammottettix alienus (Dahlbom), Macrosteles sexnotatus (Fall?n), Ophiola decumana (Kontkanen), Errastunus ocellaris Fall?n, and Scaphoideus titanus Ball; c) three species of the subfamily Typhlocibinae: Eupteryx atropunctata (Goeze), Eupteryx mellissae Curtis, Zyginidia pullula (Boheman). Female specimens of the genus Euscelis (Deltocephalinae) were also collected, as well as one species of Reptalus quinquecostatus (Dufour) of the family Cixiidae. Stolbur phytoplasmas were detected in A. laevis, A. ribauti, A. venosa, P. striatus, P. confinis and P. alienus. The species: A. laevis, O. decumana, and P. confinis were AY-infected (subgroup 16SrI-A), while subgroup 16SrI-C was found only in one specimen of P. confinis. Since some aster yellows- and stolbur-infected species of the genera Psammotettix and Anaceratagallia (especially P. confinis and A. laevis) were regularly and commonly found in the infected carrot fields during the whole vegetative period, they could play a significant role in transmitting and spreading these pathogens in natural environment.

Análise faunística e flutuação populacional de cigarrinhas (Hemiptera, Cicadellidae) em pomar cítrico no município de Chapecó, Santa Catarina

Faunistic analysis and population fl uctuation of leafhoppers (Hemiptera: Cicadellidae) in citrus orchards at Chapecó, Santa Catarina, Brazil. In order to characterize the citrus orchard ecosystems of the western region of Santa Catarina state in relation to the Cicadellidae species, faunistic analysis was carried out in Chapecó from August to July 2005. To characterize the lea- fhopper population of this municipality, the abundance index, constancy, dominance and frequency were analyzed. The survey was made using yellow tray traps. Dilobopterus costalimai, Acrogonia citrina and Scopogonalia subolivacea were the most abundant, constant, frequent and dominant species, and were therefore considered as predominant. Dilobopterus costalimai had two population peaks, one in August and another in December-January. Acrogonia citrina had two population peaks, one in August and another in January-February. In contrast, S. subolivacea presented only a single population peak in period December-January.

Ten polymorphic microsatellite markers for Scaphoideus titanus, the vector of flavescence dorée phytoplasma

AbstractThe leafhopper Scaphoideus titanus is a vector of flavescence dorée phytoplasma, the causal agent of the most important grapevine yellow disease in European vineyards. Ten polymorphic microsatellite loci were developed from a genomic library enriched for AC and AG repeats. Levels of polymorphism were evaluated in 106 individuals from S. titanus European and American populations. An average of 16 alleles per locus was detected and the observed heterozygosity ranged from 0.141 to 0.813. Cross‐species amplification was successful in four other Cicadellidae species. These 10 microsatellites are valuable markers for population genetic and phylogeographical studies of S. titanus.

Espécies de cigarrinhas (Hemiptera, Cicadellidae, Cicadellinae) em pomares de citros da região de Bebedouro, SP, com descrição de uma espécie nova de Acrogonia Stål

Sharpshooter leafhoppers (Hemiptera, Cicadellidae, Cicadellinae) in citrus groves of the area of Bebedouro, SP, with description of a new species of Acrogonia Stal. Sixteen species of Cicadellidae, subfamily Cicadellinae, were collected and identified: 11 of the tribe Cicadellini and five of the tribe Proconiini. The ten most common species, together with a new species, are characterized based on external morphology and male genitalia: Acrogonia citrina Marucci & Cavichioli sp. nov.; Bucephalogonia xanthophis (Berg, 1879); Dilobopterus costalimai Young, 1977; Ferrariana trivittata (Signoret, 1854); Hortensia similis (Walker, 1851); Macugonalia cavifrons (Stal, 1862); M. leucomelas (Walker, 1851); Oncometopia facialis (Signoret, 1854); Plesiommata corniculata Young, 1977 and P. mollicella (Fowler, 1900). The leafhopper samples were collected in citrus groves and in adjacent habitats (woods, swamp, cultivable plain, spontaneous vegetation of the grove, coffee crop and sugarcane) at the counties of Bebedouro and Monte Azul Paulista and in the district of Turvinia. The collecting methods were: insect net, yellow sticky cards, portable suction trap and Malaise trap. Identification keys were elaborated for the 16 species. There is a great diversity of Cicadellinae species in the citric groves. In the three areas sampled, it was collected a larger number of species of the tribe Cicadellini than of the tribe Proconiini. In the Bebedouro area, the vector species of Xylella fastidiosa, A. citrina sp. nov., B. xanthophis , D. costalimai and O. facialis , were collected in the citrus groves and in all adjacent habitats.