Amorphophallus Species Research Articles

Complete chloroplast genome and phylogenetic analysis of Amorphophallus paeoniifolius (Araceae)

Amorphophallus paeoniifolius (Dennst.) Nicolson, 1885, often known as elephant foot yam, is a tropical tuber crop that originates from south-east Asia and belongs to the Araceae family. It is known for its high production potential and popularity as a medicinal plant. However, the phylogeny and genes for this species are still unavailable. In this study, the first complete chloroplast genome of A. paeoniifolius was reported and phylogenetic analysis was conducted with Araceae species. The chloroplast genome was 176,258 bp in length with 34.80% overall GC content and includes a large single-copy (LSC) region (93,951 bp), a small single-copy (SSC) region (15,013 bp), and a pair of inverted repeat (IRs) regions (33,647 bp). The chloroplast genome has 130 genes, which include 85 protein-coding genes, 37 tRNA genes, and eight rRNA genes. A maximum-likelihood (ML) phylogenetic analysis indicated that all Amorphophallus species formed a single monophyletic clade with a high bootstrap value and A. paeoniifolius was closely related to A. konjac, A. albus, A. krausei, and A. titanum. The chloroplast genome reported in this study will be useful for further taxonomic and evolutionary studies of Amorphophallus.

The complete chloroplast genome assembly of Amorphophallus tonkinensis Engler and Gehrmann 1911 from southwestern China

Species in the Amorphophallus genus are important cash crops in many tropical and subtropical Asian countries. Although several molecular markers have been employed to determine relationships and assess genetic variation in the Amorphophallus genus, some conflicts remain in infrageneric classification and evolution. To aid in the phylogenetic research of the Amorphophallus genus, we collected one sample of Amorphophallus tonkinensis Engler and Gehrmann 1911 from southwestern China. We assembled the first chloroplast genome of this species using high-throughput sequencing. The assembled genome was 169,341 bp long with a typical quadripartite structure (GenBank accession number: PP234804). The lengths of the large single-copy region, small single-copy region, and two inverted repeats were 90,705 bp, 15,640 bp, 31,498 bp, and 31,498 bp, respectively. We annotated 129 genes across the chloroplast genome of A. tonkinensis. The phylogenetic trees suggested that the Amorphophallus species distributed in continental Asia split into two main clades. The chloroplast genome reported in our study provided valuable genomic resources for the future phylogenetic research of the Amorphophallus genus.

Assembly and analysis of the chloroplast genome of Amorphophallus kachinensis Engler & Gehrmann (Araceae) from Southwestern China: implications for conservation and utilization

Konjac glucomannan consists of D-mannose and D-glucose units and is a hydrocolloid obtained from the corm of Amorphophallus species. Due to its bioactive properties, biodegradability, and hydrophilic ability, glucomannan is widely used in the fields of food, medicine, and industry. Amorphophallus species have been cultivated as cash crops in many Asian countries. Amorphophallus kachinensis Engler & Gehrmann 1911 is naturally distributed in southwestern China, Laos, and northern Thailand. To help the genetic assessment and conservation of this species, the first chloroplast genome of A. kachinensis was sequenced on the Illumina sequencing platform. We assembled the chloroplast genome using the software GetOrganelle and annotated the genome by Geseq and Cpgavas 2. The assembled chloroplast genome was 173,330 bp long, and the average GC content was 35% (GenBank accession number: PP072244). The chloroplast genome of A. kachinensis contained one large single copy, one small single copy, and two inverted repeats, with lengths of 92,030 bp, 15,118 bp, 33,091 bp, and 33,091 bp, respectively. We successfully annotated 132 genes across the genome, which was consistent with other Amorphophallus species. The phylogenetic tree indicates a sub-divergence in the Amorphophallus genus with two main genetic groups detected among eight species. The two genetic groups should be treated as distinct evolutionarily significant units when making conservation strategies. Our study enriched the chloroplast genome resources of the Amorphophallus genus and could help future phylogeographic studies, protection, and utilization of wild resources.

Habitat preferences of Amorphopahllus sp. Silaiya village, Sayur Matinggi District, North Sumatra, Indonesia

Amorphophallus is included in the understory plants, belonging to the Araceae family. Amorphophallus grows wild under agroforestry stands in North Sumatra, which in the last period of time its tubers have economic value. Amorphophalus tubers can be used as food ingredients and also medicines. This research was conducted in Silaiya Village, Sayur Matinggi District, North Sumatra Province, Indonesia. The purpose of this research is to determine the habitat conditions of Amorphophallus sp that grow wild. This information aims as basic data in insitu conservation efforts. The method of this research is to conduct vegetation analysis activities using nested plots measuring 1 ha, where there are sub-plots. There are two species of Amorphophallus found in Silaiya Village, namely Amorphophallus gigas and Amorphophallus paeoniifolius. The number of individuals of Amorphophallus gigas was 120, and the number of individuals of Amorphophallus paeoniifolius was 148. The conservation status of Amorphophallus paeoniifolius is Least Concern, while the conservation status of Amorphophallus gigas has not been registered.

An updated identification guide to the species of Amorphophallus (Araceae): new synonyms and a set of global dichotomous keys

This work presents updated dichotomous keys to the Amorphophallus (Araceae) species of the world based on morphological characters of the inflorescence. It is a compilation of data derived from herbarium specimens, living specimens, protologues, and following papers outlining taxonomic updates to the genus. Three new synonyms are proposed and a general key including 239 species of Amorphophallus distributed across the African, Asian, and Australian continents is included. Ten additional subkeys are provided by geographical subregion, making identification streamlined if the locality of the specimen is known. The aim of this paper is to make identification of Amorphophallus species accessible through a single manuscript, serving not only the research sector, but also citizen science. This guide provides a rapid and accurate means of species identification, directly benefiting data collection and assisting in species conservation.

The complete chloroplast genome assembly of Amorphophallus krausei Engler, Pflanzenr 1911 (Araceae) from southwestern China

Plants in the genus Amorphophallus, many of which possess high konjac glucomannan content, are considered important cash crops in many Asian countries. Wild relatives of cultivated Amorphophallus species are valuable resources for the genetic improvement of these crops. To aid in future genetic research of wild germplasm resources of Amorphophallus, a single individual of Amorphophallus krausei Engler, Pflanzenr 1911 was collected from southwestern China, and its chloroplast genome was sequenced using next-generation sequencing technologies. The assembled chloroplast genome was 172,418 bp in length with a GC content of 35.23% (GenBank accession no. OR416863). A typical quadripartite structure was found in the genome, which was comprised of one large single-copy (LSC), one small single-copy (SSC), and two inverted repeats (IRs), with lengths of 91,983 bp, 15,591 bp, 32,422 bp, and 32,422 bp, respectively. A total of 132 genes were annotated in the genome, including 86 protein-coding genes, 38 tRNAs, and 8 rRNAs. A maximum likelihood (ML) tree of A. krausei and 17 other species in the family Araceae suggested that all Amorphophallus species formed a single monophyletic clade. A close relationship among A. konjac, A. albus, and A. krausei was also revealed by the phylogenetic tree. The newly sequenced chloroplast genome of A. krausei will support future genetic studies, particularly the assessment of genetic diversity, resource conservation, and phylogeographic research.

Characterization of the complete chloroplast genome assembly of Amorphophallus yunnanensis Engler, Pflanzenr (Araceae) from southwestern China

Southwestern China is a biodiversity hotspot due to its diverse topography and environment. Amorphophallus yunnanensis is a species of perennial herb that is mainly distributed throughout southwestern China. The genetic diversity and divergence in this species have not been assessed largely due to a lack of genomic resources. To help with the phylogeographic study, we sequenced and assembled the first complete chloroplast genome sequence of A. yunnanensis. The length of the chloroplast genome was 164,417 bp, with an average GC content of 36% (GenBank accession no. OR400247). The genome possessed a typical quadripartite structure, and the lengths of the large single-copy (LSC), small single-copy (SSC), and two inverted repeat (IR) regions were 92,149 bp, 15,182 bp, 28,543bp, and 28,543bp, respectively. A total of 128 genes were annotated across the genome, including 82 protein-coding genes, 8 rRNAs, and 38 tRNAs. The maximum likelihood (ML) phylogeny confirmed the phylogenetic position of Amorphophallus within Araceae, with the Amorphophallus species forming a single monophyletic clade with a high bootstrap value. The ML tree also indicated that A. yunnanensis was most closely related to A. coaetaneus. This newly sequenced chloroplast genome assembly will aid in future studies of genetic diversity, historical population dynamics, and geographic differentiation patterns of A. yunnanensis.

Amorphophallus muelleri blume diversity based on morphological characteristics in South Sulawesi

Porang is an alternative carbohydrate source plant that contains the highest glucomannan among other Amorphophallus species in Indonesia. Amorphophallus muelleri Blume is an important species of the genus Amorphophallus, which belongs to the same subspecies A. bulbifer and A. yuloensis. A. muelleri Blume has good disease resistance and heat tolerance tendency, and high KGM content. Kinship relationships can be used as a basis for assembling more potential genotypes. To find out the kinship between existing varieties we must exploration and identify. This research was carried out using qualitative methods analysed in the numerical taxonomy and multivariate analysis system version 2.02i (NTSYS) program. The genetic materials used in this study were explored from wild growing habitat in agroforestry areas in South Sulawesi, Indonesia. The results obtained that the MR6 and BL10 genotypes which had a similarity coefficient value of 1.00, between genotypes MR4, MR9, MR10, BT4 and BT10 had a similarity coefficient value of 1.00, between genotypes BT5 and BT7 have a similarity coefficient value of 1.00, and between genotypes BT1, BT2, BT9 have a similarity coefficient value of 1.00. Furthermore, BL1, BL4, and BL6 contribute significantly of the variation as showed by PC1.

A new species of Amorphophallus (Araceae) from northeastern Thailand.

Amorphophallussakonnakhonensis Chatan & Promprom, a new species from northeastern Thailand, is described and illustrated. The new species is most similar to A.harmandii Engl. & Gehrm. and A.linearis Gagnep., but it is distinguished by the combination of characters as follows: clear differences with A.harmandii are shorter style; disc-like, slightly smooth surface, concave centre, ca. 0.2 × 0.1 mm stigma; slightly cylindrical, slightly narrower upper part of staminate flower zone; slightly cylindrical to elongate-fusiform, erect or slightly erect, creamy white appendix. The clearly distinct morphology with A.linearis are disc-like, slightly smooth surface, concave centre, ca. 0.2 × 0.1 mm stigma; elliptic or obovate leaflet; 1-3 cm long, creamy white appendix. The preliminary conservation status was assessed, and the distinct characteristics of similar species were discussed.

The complete mitochondrial genome of Amorphophallus albus and development of molecular markers for five Amorphophallus species based on mitochondrial DNA.

Amorphophallus albus is an herbaceous, cormous, perennial plant used as a food source and traditional medicine in Asia. In this study, we assembled and annotated the complete mitochondrial genome (mitogenome) of A. albus. Then we analyzed the repeated elements and mitochondrial plastid sequences (MTPTs), predicted RNA editing sites in mitochondrial protein-coding genes (PCGs). Lastly, we inferred the phylogenetic relationships of A. albus and other angiosperms based on mitochondrial PCGs, and designed two molecular markers based on mitochondrial DNA. The complete mitogenome of A. albus consists of 19 circular chromosomes. And the total length of A. albus mitogenome is 537,044 bp, with the longest chromosome measuring 56,458 bp and the shortest measuring 12,040 bp. We identified and annotated a total of 36 protein-coding genes (PCGs), 21 tRNA genes, and 3 rRNA genes in the mitogenome. Additionally, we analyzed mitochondrial plastid DNAs (MTPTs) and identified 20 MTPTs between the two organelle genomes, with a combined length of 22,421 bp, accounting for 12.76% of the plastome. Besides, we predicted a total of 676 C to U RNA editing sites on 36 protein-coding genes of high confidence using Deepred-mt. Furthermore, extensive genomic rearrangement was observed between A. albus and the related mitogenomes. We conducted phylogenetic analyses based on mitochondrial PCGs to determine the evolutionary relationships between A. albus and other angiosperms. Finally, we developed and validated two molecular markers, Ai156 and Ai976, based on two intron regions (nad2i156 and nad4i976) respectively. The discrimination success rate was 100 % in validation experiments for five widely grown konjac species. Our results reveal the multi-chromosome mitogenome of A. albus, and the developed markers will facilitate molecular identification of this genus.

Patterns and drivers of heat production in the plant genus Amorphophallus.

Thermogenesis - the ability to generate metabolic heat - is much more common in animals than in plants, but it has been documented in several plant families, most prominently the Araceae. Metabolic heat is produced in floral organs during the flowering time (anthesis), with the hypothesised primary functions being to increase scent volatilisation for pollinator attraction, and/or to provide a heat reward for invertebrate pollinators. Despite in-depth studies on the thermogenesis of single species, no attempts have yet been made to examine plant thermogenesis across an entire clade. Here, we apply time-series clustering algorithms to 119 measurements of the full thermogenic patterns in inflorescences of 80 Amorphophallus species. We infer a new time-calibrated phylogeny of this genus and use phylogenetic comparative methods to investigate the evolutionary determinants of thermogenesis. We find striking phenotypic variation across the phylogeny, with heat production in multiple clades reaching up to 15°C, and in one case 21.7°C above ambient temperature. Our results show that the thermogenic capacity is phylogenetically conserved and is also associated with inflorescence thickness. Our study paves the way for further investigations of the eco-evolutionary benefits of thermogenesis in plants.

Different Response Mechanisms of Rhizosphere Microbial Communities in Two Species of Amorphophallus to Pectobacterium carotovorum subsp. carotovorum Infection.

Soft rot is a widespread, catastrophic disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc) that severely damages the production of Amorphophallus spp. This study evaluated the rhizosphere bacterial and fungal communities in Pcc-infected and uninfected plants of two species of Amorphophallus, A. muelleri and A. konjac. Principal component analysis showed that the samples formed different clusters according to the Pcc infection status, indicating that Pcc infection can cause a large number of changes in the bacterial and fungal communities in the Amorphophallus spp. rhizosphere soil. However, the response mechanisms of A. muelleri and A. konjac are different. There was little difference in the overall microbial species composition among the four treatments, but the relative abundances of core microbiome members were significantly different. The relative abundances of Actinobacteria, Chloroflexi, Acidobacteria, Firmicutes, Bacillus, and Lysobacter were lower in infected A. konjac plants than in healthy plants; in contrast, those of infected A. muelleri plants were higher than those in healthy plants. For fungi, the relative abundances of Ascomycota and Fusarium in the rhizosphere of infected A. konjac plants were significantly higher than those of healthy plants, but those of infected A. muelleri plants were lower than those of healthy plants. The relative abundance of beneficial Penicillium fungi was lower in infected A. konjac plants than in healthy plants, and that of infected A. muelleri plants was higher than that of healthy plants. These findings can provide theoretical references for further functional research and utilization of Amorphophallus spp. rhizosphere microbial communities in the future.

First report of rust caused by Puccinia paullula on Monstera deliciosa and M. adansonii in Japan.

Monstera deliciosa Liebm. and M. adansonii Schott. (Araceae) have been cultivated for the commercial production in Hachijo Island, Tokyo, Japan, since the 1950s. A rust disease producing uredinial sori was found on the leaves of M. deliciosa and M. adansonii in the fields on Hachijo Island in February 2021. Symptoms were first observed on the leaf surfaces as small chlorotic spots, which expanded and became brown to reddish-brown, and produced uredinia with abundant urediniospores. The disease occurred on the whole island including the farm land, symptom incidence ranged from 5 to 30%. To confirm the pathogenicity of this rust fungus, ten plant species of Araceae (M. deliciosa, M. adansonii, Alocasia macrorrhizos, Anthurium andreauum, Dieffenbachia maculata, Epipremnum mirabile, E. pinnatum, Philodendron scandens, Spathiphyllum sp., Syngonium podophyllum) were inoculated with urediniospores obtained from infected samples on M. deliciosa and M. adansonii. Urediniospores were suspended in distilled water (1 × 106 conidia/ml) and sprayed on healthy plants. The inoculated plants were kept in a dark chamber at about 25°C for 48 h, and then transferred to a greenhouse. After 40 days, uredinia were reproduced only on M. deliciosa and M. adansonii. Morphological characteristics of the urediniospores obtained from inoculated monstera plants matched those obtained from the field plants and used as inoculum, thus fulfilling Koch's postulates. No symptoms were observed on the other plant species inoculated. For identification of the rust fungus, dry specimens obtained from both naturally infected plants and inoculated plants were used for morphological observations. Urediniospores with pedicels emerging from the stomata were densely aggregated, globose, and 24.8-29.3 µm (n = 30). Their walls were brown, echinulate, and 1.4-2.2 µm thick. Telia were found in the specimens collected in July and August. The teliospores with pedicels emerging from the stomata were two-celled, ellipsoid, and 21.3-27.5 × 19.5-23.4 µm. Their walls were pale yellow, smooth, and 1.0-1.9 µm thick. Morphological differences among the specimens on M. deliciosa and M. adansonii were not observed. These morphological characteristics were consistent with the description of P. paullula (Sydow and Sydow 1913). For molecular identification, the large subunit (LSU) rDNA and cytochrome oxidase III (cox3) were amplified. LRust1R, LR3, and LR5 (Vilgalys and Hester 1990) primers were used for sequencing of LSU region. Cox3_F (5'-GTTCAGTATGTTATTTTAACG-3') and cox3_R (5'-ATAGGAATAGTCAAACAACATC-3') primers were constructed here based on the P. paullula sequences (KX999927) for the cox3 region. The sequences were deposited in the GenBank as accession numbers OK509070, OK509071, LC663719 and LC663720. BLAST analysis showed that LSU and cox3 sequences shared 98.8% (902/913 nts) and 100% (632/632 nts) identities with P. paullula, KX999886 and KX999927 (Marin-Felix et al. 2017). This is the first report of a rust disease of M. deliciosa and M. adansonii caused by P. paululla in Japan. The rust fungi has been reported as the pathogen on the species of Amorphophallus, Rhaphidophora and Monstera (Sydow and Sydow 1913, Shaw 1991, Chen et al. 2009). The occurrence of this disease should be monitored because it can reduced yield and commercial value of monstera plants. All the specimens used in the experiments were deposited in the National Museum of Nature and Science, Japan (TNS-F82068-82077).

Taxonomic studies of Araceae in Myanmar IV: A new species, a new record and a new synonym for the genus Amorphophallus

Amorphophallus wasa Naive, K. Z. Hein & Hett., is described and illustrated as a species new to science from the Sagaing Region, Myanmar. It is morphologically similar to A. saraburiensis Gagnep., but can be easily distinguished by its unilocular ovaries and lack of staminodes between pistillate and staminate zones. A detailed description, colour plates, phenology, distribution map, provisional conservation status and a key to the Amorphophallus species from Myanmar are provided. In addition, A. elatus Hook. f. is reported as a newly recorded species for Myanmar, and the name A. corrugatus N.E.Br. is newly synonymized under A. kachinensis Engl. & Gehrm.

Identification of the generative phases of Amorphophallus gigas in the Sabungan Village and Langga Payung Village, Sungai Kanan District, North Sumatra Province, Indonesia

Amorphophallus gigas is one of Sumatra's endemic Amorphophallus species that is protected in Indonesia. Amorphophallus gigas is one type of Amorphophallus with the highest flowers in the world. The conversion of land to other uses is one of the causes of the reduced population of Amorphophallus gigas. One of the natural habitats of Amorphophallus gigas was found in the Sabungan Village and Langga Payung Village, North Sumatra. The purpose of this study was to determine the distribution of the generative phase of Amorphophallus gigas in the Sabungan Village and Langga Payung Village, to determine the types of plants found around the place of growth and to determine the morphology of the generative phase of Amorphophallus. The method used in this research is the roaming method and then records the coordinates of the generative phase of Amorphophallus, records the types of pollinating insects around it and observes the morphology of Amorphophallus gigas. The results showed that there were 11 individuals in the generative phase of Amorphophallus gigas in the Sabungan Village and Langga Payung Village, where 10 individuals grew on rubber land cover and 1 individual grew on oil palm land cover. A total of 2 individuals were found in bud condition, 1 individual was in bloom, 2 individuals were wilted and 6 individuals were found at the seed formation stage. There are 12 types of insects found around the generative phase of the Amorphophallus.

Weighted Gene Coexpression Network Analysis of Candidate Pathways and Genes in Soft Rot Resistance of Amorphophallus

Amorphophallus species are one of the main economic crops in the mountainous areas of southwest China. However, soft rot disease (Pectobacterium carotovorum ssp. carotovorum) is devastating for this crop. This study explored the Amorphophallus resistance mechanism against soft rot disease by analyzing transcriptome data using a weighted gene coexpression network analysis. The RNA sequencing of plants infected for 0, 12, 24, and 48 hours produced a total of 52.25 Gb of clean reads. A total of 29,096 genes were divided into 34 modules. Six modules of interest with the highest correlation with the target traits were selected to elucidate the resistance genes and pathways. The selected modules were enriched in the α-linolenic acid metabolism, phenylpropane biosynthesis, plant hormone signal transduction, and plant pathogen interaction pathways. Ultimately, AmBGLU, AmCAML, AmCDPK, AmLOX, and AmRBOHD were identified as genes of interest in the four significantly related metabolic pathways for real-time fluorescence quantitative polymerase chain reaction verification. The determination of salicylic acid (SA) and jasmonic acid (JA) in Amorphophallus muelleri and Amorphophallus konjac that suffered from soft rot disease showed that SA and JA were involved in the A. muelleri and A. konjac defense response against soft rot disease. Methyl jasmonate treatment delayed the onset of A. konjac soft rot disease. This study provides a reference for the interaction between Amorphophallus species and soft rot disease and the breeding of broad-spectrum and specific Amorphophallus cultivars that are resistant to soft rot disease.

A new species of Amorphophallus (Araceae) from Eastern D.R. Congo, and a new record of the genus from Rwanda

Background and aims – A new species of Amorphophallus (Araceae) is described from D.R. Congo in connection with preparing the family treatment for the Flore d’Afrique centrale. Another species is recorded for the first time from Rwanda. Methods – Standard herbarium practices were applied. Key results – Amorphophallus dumboi sp. nov. is related to A. margretae. The differences between these species are discussed and distribution maps for the taxa are presented. Both species are range-restricted in the Albertine Rift and preliminarily assessed as Critically Endangered. Amorphophallus mayoi is for the first time recorded for Rwanda. The taxon, originally described as a subspecies of A. calabaricus, is raised here to specific rank.

Metabolic Profiling of Three Species of Amorphophallus (Araceae)

A study was conducted to determine the metabolic contents of corms of three species of Amorphophallus, A. muelleri, A. paeoniifolius, and A. variabilis using gas chromatography mass spectrometry (GCMS) with water as polar solvent. The corms were collected from plants cultivated at the Leuwikopo Experimental Station, IPB University, Darmaga, Bogor, Indonesia. Metabolic profiling conducted at the Jakarta Regional Health Laboratory, Indonesian Ministry of Health, demonstrated that the three species of Amorphophallus vary in metabolic content, specifically for 12 compounds belonging to antioxidants, vitamins, saturated fatty acids, polyunsaturated fatty acids, phytosterol, alcohol, aldehyde, and alkane. Our study had shown that metabolic profiling is a potentially useful method of differentiating or determining species markers that in turn could be efficient way for genetic conservation and development of future food source.

Notes on the genus Amorphophallus Blume ex Decne. (Araceae) of northwestern Luzon, Philippines, including a new species

A botanical exploration conducted by the Northwestern University – Biodiversity Research Unit and other collaborating institutions in the Ilocos Region, Northwestern Luzon, Philippines, led to the documentation of five Amorphophallus species in the area. Here, we provide new distributional records for A. longispathaceus and A. urceolatus, an expanded description and new conservation status for A. adamsensis, and describe new color forms of A. paeoniifolius. Moreover, the new species Amorphophallus flammeus is described and compared to its closest relatives.

Population Distribution of Amorphophallus at Several Altitudes in Mount Poteng, Raya Pasi Nature Reserve, West Kalimantan

Amorphophallus is a protected and endangered plant. Some Amorphophallus species can only grow in certain environmental conditions. Differences in environmental conditions could cause differences in morphology and distribution of Amorphophallus. This study aimed to obtain the morphological characteristics of Amorphophallus and study the distribution of its population at several altitudes in Mount Poteng, Raya Pasi Nature Reserve, West Kalimantan. The study used an exploratory survey following the river’s flow at the Raya Pasi Nature Reserve, especially in Mount Poteng. The results found three species of Amorphophallus: Amorphophallus borneensis (109 individuals), Amorphophallus gigas (9 individuals), and Amorphophallus hewittii (27 individuals), showing clustered distribution patterns with a Morista index of > 1. The similarity index of six comparisons for each altitude showed five altitudes with similar categories and one altitude with a very different category. The results revealed that the Amorphophallus distribution positively correlated with air temperature and soil temperature but negatively correlated with air humidity. However, all correlations were weak. The morphological differences of the three Amorphophallus species were in the forms of the stem pattern, stem height, and fruit shape. The distribution of Amorphophallus can be found at an altitude of 200 – > 500 masl, which is not far from the river flow. Keywords: altitude, Amorphophallus, Mount Poteng, Raya Pasi Nature Reserve