Species Groups Research Articles

The Application Timing of a Cytokinin B-Mo-based Product Affects the Characteristics of Rooted Cuttings and Nonstructural Carbohydrates of Coleus (Plectranthus scutellarioides cv. Wild Lime) during Adventitious Root Development

A large group of horticulture species are propagated vegetatively through shoot-tip cuttings harvested from stock plants and planted to form adventitious roots. Adventitious rooting leads to establishing a carbohydrate sink in the region of root regeneration that is highly dependent on energy and carbon skeletons. We hypothesized that the timing of exogenous applications of cytokinin (CK) and boron (B)–molybdenum (Mo)-based products during adventitious root development can affect the flow of sugars from leaves to sinks, carbon allocation to the adventitious roots, and the quality of rooted cuttings. During this project, we aimed to determine if the application time of a CK/B-Mo-based product during the adventitious root development of unrooted cuttings would impact the source-to-sink relationship and, hence, affect plug growth and quality. A sink-strengthening commercial product based on cytokinin, B, and Mo was applied at four plug development stages plus a negative control as follows: T1, plants without product (control); T2, sticking stage (starting 24 hours after the sticking); T3, callus formation stage; T4, root development stage; and T5, toning stage. The root and shoot lengths and dry matter, number of leaves, leaf chlorophyll content, root-to-shoot ratio (based on dry matter), and nonstructural carbohydrate contents were measured. The timing of the application of the product impacted the root development, quality of the cuttings, and nonstructural carbohydrate content. Product application during the adventitious root dedifferentiation and induction phases (T2) resulted in the shortest root and shoot lengths, lowest dry matter accumulation, lowest nonstructural carbohydrate contents, and some phytotoxicity. Application during the initiation phase (T3) resulted in greater root length, total dry matter, and total soluble sugar contents compared with the control. Application during the expression phase (T4) resulted in the largest root length and mass and the highest sucrose contents. Applying the product when the roots had grown and reached all the edges of the growing media (T5) did not have any benefits compared with the control. This study provides new insights into the application timing of exogenous CKs, B, and Mo to generate a well-toned rooted coleus cutting and potential explanations in relation to nonstructural carbohydrate metabolism.

Phylogenetic diversity of the Pristimantis anaiae species group, with a description of a new species of Pristimantis (Anura, Strabomantidae) from Cerro Candelaria, Tungurahua, Ecuador

Continuous field work on mountains in the upper Pastaza watershed, showed an unexpected cryptic diversity in the recently described Pristimantis anaiae species group. Based on molecular and morphological characters we describe a new frog species from the central region of the eastern Ecuadorian Andes. We present an updated phylogeny for the Pristimantis anaiae species group, based on widely used, ubiquitous, and available genes, three mitochondrial genes (12S rRNA, 16S rRNA, ND1) and RAG1 nuclear gene. Our phylogenetic hypothesis increases genetic diversity of the species group and shows two well-supported subclades. We show the relationships of the new species and include for the first time Pristimantis maryanneae, belonging to a clade related to P. resistencia + P. glendae. Our phylogeny shows that new species, from Cerro Candelaria, is more closely related to P. kunam from Sangay National Park and differs from it by having a less conspicuous dorsolateral row of enlarged tubercles, and sacral oval marks surrounded by thin white pale lines. The new species and two additional undescribed species occur on the south side of the Rio Pastaza Valley, at Cerro Candelaria within the Llanganates – Sangay Ecological Corridor, reflecting the high importance of this area for conservation and understanding of evolution processes in the area, suggesting a probably adaptive radiation zone for the P. anaiae species group.

Contribution to the Chinese Intybia Pascoe, 1866 (Coleoptera, Melyridae, Malachiinae), with descriptions of two new species groups and one new species

Contribution to the Chinese Intybia Pascoe, 1866 (Coleoptera, Melyridae, Malachiinae), with descriptions of two new species groups and one new species

Identification of main variables in forest management for recovery of endemic species habitat in burned areas of southern Chile

Wildfires are among the most serious disturbances that affect forest biodiversity, damaging both flora and fauna. In some burned areas, vegetation recovery is a long and complex process that requires the support of restorative actions. This study evaluated changes in forest structural features after a fire that affect habitat suitability for Rhinocryptids, an endemic group of bird species that are indicators of biodiversity in the temperate forests of South America. The study area was the Malleco National Reserve, Chile, where the main ecosystems are temperate forests. Four species from the Rhinocryptidae family of birds were used as bioindicator species. This group of species is endemic to temperate forests in South America. After fires occurred in 2002 and 2015, affecting temperate forests dominated by several Nothofagus species, two field inventories were carried out in 2016 and 2018. These inventories recorded the presence of Rhinocryptids and some forests structural features. Differences between the two stages of forest regeneration were assessed using statistical tests (T-test and Wilcoxon–Mann–Whitney test) and a classification model was proposed to determine the presence of Rhinocryptidae species. The statistical analysis of forest structural features showed that the variables more relevant in the habitat recuperation are vertical stratification (p-value < 0.001) and understory cover (p-value = 0.053). The trained decision tree to predict the presence of Rhinocryptidae species had an accuracy of 65.22%. This model showed that the coexistence of more than one Rhinocryptidae species occurs when vertical stratification is not mono-stratified and the density is lower than 335 trees ha−1. Our results can help forest managers to consider forest structure as a suitable habitat for certain major wildlife species when implementing post-fire recovery plans.

Insights into the Crystallinity-Dependent Photochemical Productions of Reactive Oxygen Species from Iron Minerals.

Iron minerals are widespread in earth's surface water and soil. Recent studies have revealed that under sunlight irradiation, iron minerals are photoactive on producing reactive oxygen species (ROS), a group of key species in regulating elemental cycling, microbe inactivation, and pollutant degradation. In nature, iron minerals exhibit varying crystallinity under different hydrogeological conditions. While crystallinity is a known key parameter determining the overall activity of iron minerals, the impact of iron mineral crystallinity on photochemical ROS production remains unknown. Here, we assessed the photochemical ROS production from ferrihydrites with different degrees of crystallinity. All examined ferrihydrites demonstrated photoactivity under irradiation, resulting in the generation of hydrogen peroxide (H2O2) and hydroxyl radical (•OH). The photochemical ROS production from ferrihydrites increased with decreasing ferrihydrite crystallinity. The crystallinity-dependent photochemical •OH production was primarily attributed to conduction band reduction reactions, with the reduction of O2 by conduction band electrons being the rate-limiting key process. Conversely, the crystallinity of iron minerals had a negligible influence on photon-to-electron conversion efficiency or surface Fenton-like activity. The difference in ROS productions led to a discrepant degradation efficiency of organic pollutants on iron mineral surfaces. Our study provides valuable insights into the crystallinity-dependent ROS productions from iron minerals in natural systems, emphasizing the significance of iron mineral photochemistry in natural sites with abundant lower-crystallinity iron minerals such as wetland water and surface soils.

Culture-based diversity of endophytic fungi of three species of Ferula grown in Iran

A total of 1,348 endophytic fungal strains were isolated from Ferula ovina, F. galbaniflua, and F. persica. They included Eurotiales (16 species), Pleosporales (11 species), Botryosphaeriales (1 species), Cladosporiales (2 species), Helotiales (6 species), Hypocreales (31 species), Sordariales (7 species), Glomerellales (2 species), and Polyporales (1 species). F. ovina had the richest species composition of endophytic fungi, and the endophytic fungi were most abundant in their roots compared to shoots. Chao, Margalef, Shannon, Simpson, Berger–Parker, Menhinick, and Camargo indices showed that F. ovina roots had the most endophytic fungal species. The frequency distribution of fungal species isolated from Ferula spp. fell into the log-series model, and F. ovina roots had the highest Fisher alpha. The dominance indices showed that there are no dominant species in the endophytic fungal community isolated from Ferula spp., indicating community stability. Evenness values were 0.69, 0.90, 0.94, and 0.57 for endophytic fungi isolated from F. ovina roots, F. ovina shoots, F. galbaniflua roots, and F. persica roots, respectively, indicating a species distribution that tends toward evenness. The fungal species community isolated from each of F. ovina roots, F. ovina shoots, F. galbaniflua roots, and F. persica roots was a diverse species group originating from a homogeneous habitat. Their distribution followed a log-normal distribution, suggesting that the interactions of numerous independent environmental factors multiplicatively control species abundances. Principal component analysis showed that the highest species diversity and dominance were observed in the endophytic fungal community isolated from F. ovina and F. persica roots, respectively.

Description of Trypoxylon sicklum sp. nov. from Vietnam and a key to species in the Trypoxylon fulvocollare group (Hymenoptera, Crabronidae)

Trypoxylon Latreille, 1796 (Hymenoptera, Crabronidae) consists of 633 known species worldwide and the genus is divided into numerous species groups. In Vietnam, 19 species of Trypoxylon have been recorded to date. In this study, a new species, Trypoxylon sicklum Pham and Antropov sp. nov. is described and illustrated from Vinh Phuc Province, Vietnam. The new species is the second member of the Trypoxylon fulvocollare species group. A key to species in the Trypoxylon fulvocollaris group is presented. Trypoxylon sicklum Pham and Antropov sp. nov., is described as a new species and is the second member of the Trypoxylon fulvocollare species group. A key to species in the Trypoxylon fulvocollaris group is presented.

Psychotria pendens (Rubiaceae, Psychotrieae), a new ultramafic species from Central Palawan, Philippines

Psychotria pendens from the lowland ultramafic forest of Mt. Victoria, Palawan Island, Philippines is herein described as a new species of Rubiaceae. The new species is morphologically similar to P. rubiginosa (Gitingensis species group) by having monochotomous inflorescence and oblanceolate to oblong-elliptic leaves but is distinguished by its large, leaf-like, valvate stipules with biattenuate to biacuminate tips, pendent inflorescence, glabrous abaxial leaf surface, narrowly triangular bracts, and glabrous calyx. Molecular analysis revealed that this newly discovered species belongs to the Pacific Psychotria clade, and is allied to the Philippine species P. luzoniensis and Hawaiian species P. mariniana. The species is assessed as Critically Endangered (CR) based on the IUCN Red List categories and criteria.

Morphological Characterization of Fossil Vitis L. Seeds from the Gelasian of Italy by Seed Image Analysis.

The discovery of well-preserved fossil Vitis L. seeds from the Gelasian stage in Italy has provided a unique opportunity to investigate the systematics of fossilized Vitis species. Through seed image analyses and elliptical Fourier transforms of fossil Vitis seeds from the sites Buronzo-Gifflenga and Castelletto Cervo II, we pointed out a strong relationship to the group of extant Eurasian Vitis species. However, classification analyses highlighted challenges in accurately assigning the fossil grape seeds to specific modern species. Morphological comparisons with modern Vitis species revealed striking similarities between the fossil seeds and V. vinifera subsp. sylvestris, as well as several other wild species from Asia. This close morphological resemblance suggests the existence of a population of V. vinifera sensu lato in Northen Italy during the Gelasian. These findings contributed to our understanding of the evolution and the complex interplay between ancient and modern Vitis species.

Biochemical Affinities of some Species of &lt;i&gt;Ficus&lt;/i&gt;

Polyphenolic constituents of eighteen Ficus species were studied as separated by paper chromatography. Their inter-relationship was examined on the basis of their paired affinity (PA) indices. A comparative relationship of each Species in respect of PA values with others, are shown in polygonal graphs. Each species posses a distinct shape and area of these polygons. F. eriobotryoides, the only species native of Tropical Africa included, has distinctly smaller PA values thereby indicating its remote relationship with others.About 25-30% of the polyphenolic constituents are common in all the species, probably an indication of common heritage. At least two phenolics are found to be common to all the species studied.PA indices between 30 and 60% are shown by a fairly large group of species in each case. Considering PA values above 60% or less than 30% as indicating more nearness or remoteness respectively, it is possible to show inter-relationship among them. The various species of the subgenus Urostigma clearly isolate themselves into one group. F. virens, F. elastica and F. drupaceae var. pubescens appear to be the connecting links between the species of the subgenus Urostigma and Ficus. Details of the relationship are discussed.

Tree demographic strategies largely overlap across succession in Neotropical wet and dry forest communities.

Secondary tropical forests play an increasingly important role in carbon budgets and biodiversity conservation. Understanding successional trajectories is therefore imperative for guiding forest restoration and climate change mitigation efforts. Forest succession is driven by the demographic strategies-combinations of growth, mortality and recruitment rates-of the tree species in the community. However, our understanding of demographic diversity in tropical tree species stems almost exclusively from old-growth forests. Here, we assembled demographic information from repeated forest inventories along chronosequences in two wet (Costa Rica, Panama) and two dry (Mexico) Neotropical forests to assess whether the ranges of demographic strategies present in a community shift across succession. We calculated demographic rates for >500 tree species while controlling for canopy status to compare demographic diversity (i.e., the ranges of demographic strategies) in early successional (0-30 years), late successional (30-120 years) and old-growth forests using two-dimensional hypervolumes of pairs of demographic rates. Ranges of demographic strategies largely overlapped across successional stages, and early successional stages already covered the full spectrum of demographic strategies found in old-growth forests. An exception was a group of species characterized by exceptionally high mortality rates that was confined to early successional stages in the two wet forests. The range of demographic strategies did not expand with succession. Our results suggest that studies of long-term forest monitoring plots in old-growth forests, from which most of our current understanding of demographic strategies of tropical tree species is derived, are surprisingly representative of demographic diversity in general, but do not replace the need for further studies in secondary forests.

Quantifying marine larval dispersal to assess MPA network connectivity and inform future national and transboundary planning efforts

A marine protected area (MPA) network of multiple reserves can protect biodiversity across space, but to be effective, network configuration should support dispersal among MPAs as well as spillover to unprotected habitats. The ability of MPAs to function as an interacting network of populations connected by dispersal, however, is difficult to estimate at broad spatial scales, and therefore, connectivity is often not integrated in MPA design. Here, we simulate passive larval dispersal using a biophysical model to estimate potential transboundary network connectivity of MPAs in western Canada and United States. Drift time was varied to represent generic functional groups of nearshore species with planktonic larvae and sedentary adult stages. We found that MPAs potentially act as an interacting network and meet connectivity design criteria for nearshore invertebrate species, many MPAs (65%–90%) possibly exchange individuals, and a third of larvae from MPAs (25%–32%) source areas of the unprotected coast. This analysis provides a first approximation of multispecies connectivity to inform ongoing transboundary MPA design, and it can be used as a foundation for future model development.

Similar looking sisters: A new sibling species in the Pristimantis danae group from the southwestern Amazon basin (Anura, Strabomantidae)

We describe a new frog species that is the sibling of Pristimantis reichlei. These two sister species inhabit the Amazonian lowlands and adjacent foothills of the Andes, from central Bolivia to central Peru. Pristimantis reichlei occurs from central Bolivia to southern Peru (Alto Purús National Park), while the new species occurs from northern Bolivia (Departamento Pando) to Panguana in central Peru (Departamento Huánuco), at elevations between 220 and 470 m a.s.l. In spite of their morphological crypsis, these siblings occur in syntopy without evidence of interbreeding (in the Alto Purús area) and are recovered as reciprocally monophyletic. Their uncorrected pairwise genetic distances in the 16S rRNA gene range from 9.5–13.5%, and their advertisement calls differ in both qualitative and quantitative traits. Moreover, our study found uncorrected pairwise distances within the new species of up to 5.0% and up to 9.3% within P. reichlei. We therefore cannot rule out the possible existence of hybrids or additional species-level lineages hidden in this complex. Furthermore, we found another potential pair of sibling species composed of P. danae and an unnamed lineage, with divergences of 9.4% in the 16S gene, whose in-depth analysis and taxonomic treatment are pending future revision. With the new nominal species, the Pristimantis danae species group now includes 20 species, distributed across the upper Amazon basin and in the eastern Andes, from western Brazil to Bolivia and Peru. Our study, together with an increasing number of other studies, indicates that sibling species are far from being rare among Amazonian amphibians and that species resolution remains low even for groups that have received considerable attention in recent years.

Three new feather mite species of the genus Proterothrix Gaud, 1968 (Analgoidea: Proctophyllodidae: Pterodectinae) from birds of paradise (Passeriformes: Paradisaeidae)

Three new feather mite species of the genus Proterothrix Gaud, 1968 (Proctophyllodidae: Pterodectinae) are described from birds of paradise (Corvida: Paradisaeidae): Proterothrix maior sp. n. and P. modestasimilis sp. n. from the Crinkle-collared Manucode, Manucodia chalybatus (Pennant, 1781), and P. papuensis from the Lesser Bird-of-paradise, Paradisaea minor Shaw, 1809. All three species belong to the wolffi species group, in having almost closed coxal fields III in males and parallel-sided terminal cleft in females.

A taxonomic review of Thiotricha Meyrick, 1886 (Lepidoptera, Gelechiidae, Thiotrichinae) from China, with descriptions of 84 new species

A taxonomic review of Thiotricha Meyrick, 1886 (Lepidoptera, Gelechiidae, Thiotrichinae) from China, with descriptions of 84 new species

Genomic analysis and phylogenetic characterization of Himalayan snow trout, Schizothorax esocinus based on mitochondrial protein-coding genes.

Mitochondrial DNA (mtDNA) has become a significant tool for exploring genetic diversity and delineating evolutionary links across diverse taxa. Within the group of cold-water fish species that are native to the Indian Himalayan region, Schizothorax esocinus holds particular importance due to its ecological significance and is potentially vulnerable to environmental changes. This research aims to clarify the phylogenetic relationships within the Schizothorax genus by utilizing mitochondrial protein-coding genes. Standard protocols were followed for the isolation of DNA from S. esocinus. For the amplification of mtDNA, overlapping primers were used, and then subsequent sequencing was performed. The genetic features were investigated by the application of bioinformatic approaches. These approaches covered the evaluation of nucleotide composition, codon usage, selective pressure using nonsynonymous substitution /synonymous substitution (Ka/Ks) ratios, and phylogenetic analysis. The study specifically examined the 13 protein-coding genes of Schizothorax species which belongs to the Schizothoracinae subfamily. Nucleotide composition analysis showed a bias towards A + T content, consistent with other cyprinid fish species, suggesting evolutionary conservation. Relative Synonymous Codon Usage highlighted leucine as the most frequent (5.18%) and cysteine as the least frequent (0.78%) codon. The positive AT-skew and the predominantly negative GC-skew indicated the abundance of A and C. Comparative analysis revealed significant conservation of amino acids in multiple genes. The majority of amino acids were hydrophobic rather than polar. The purifying selection was revealed by the genetic distance and Ka/Ks ratios. Phylogenetic study revealed a significant genetic divergence between S. esocinus and other Schizothorax species with interspecific K2P distances ranging from 0.00 to 8.87%, with an average of 5.76%. The present study provides significant contributions to the understanding of mitochondrial genome diversity and genetic evolution mechanisms in Schizothoracinae, hence offering vital insights for the development of conservation initiatives aimed at protecting freshwater fish species.

Podapolipid mites (Acari: Heterostigmata): key to genera, biogeographical history and description of four new Australian species of Eutarsopolipus

Four new species of parasitic mites of the genus Eutarsopolipus (Trombidiformes: Podapolipidae) are described from Australian carabid beetles: E. ampullaceous sp. nov. (ochoai species group) from Lecanomerus niger (Harpalini); E. tripodus sp. nov. (ochoai species group) from Notolestus sulcipennis (Pterostichini); E. vepreculus sp. nov. (leytei species group) from Coleolissus papua (Harpalini); and E. walteri sp. nov. (myzus species group) from Chlaenius ophonoides (Chlaeniini). Keys to the world genera of Podapolipidae and to the Australian species of Eutarsopolipus are provided. We further reviewed the world distribution of Eutarsopolipus species groups and postulated that the Australian representatives could be classified into two distinct faunas: a putatively Gondwanan fauna represented mainly by the ochoai species group and a more recent Laurasian fauna represented by just a few species in the myzus and acanthomus species groups.

Low temperature-mediated changes in soil free-living nitrogen fixation functional groups in tree species at different successional stages in subtropical forests

Low temperature-mediated changes in soil free-living nitrogen fixation functional groups in tree species at different successional stages in subtropical forests

Diversity of Pleurostomatid Ciliates: Morphology, Taxonomy and Molecular Phylogeny of Freshwater Isolates Found in a Northern China Wetland, with a Description of Two New Species

Ciliates of the order Pleurostomatida play essential functions in microbial food webs from a variety of habitats and have been thought to possess a high level of diversity. Due to undersampling and often absent molecular data, the actual diversity and phylogenetic relationships within this group remain unclarified. To help address this deficiency, a survey of freshwater pleurostomatid ciliates was undertaken in Lake Weishan Wetland, northern China. Here, two new Amphileptus species, Amphileptus sinicus sp. nov. and Amphileptus piscinarius sp. nov., were investigated using modern morphological and molecular techniques. Amphileptus sinicus sp. nov. is characterized by possessing a comparatively large cell size of 330–490 μm, contractile vacuoles on both ventral and dorsal margins, and 8–10 left and 42–61 right kineties. Amphileptus piscinarius sp. nov. is characterized by possessing a cell size of 140–210 μm, a large distinctly developed apical extrusome group, 3–4 contractile vacuoles on the ventral margin, and 6–8 left and 24–28 right kineties. Phylogenetic results based on the 18S rRNA gene data of these two species group them with other congeners, with these data suggesting the genus Amphileptus is paraphyletic.

Discovery of the Larvae and Pupae of the Black Fly Simulium (Gomphostilbia) khelangense and Breeding Habitats of Potential Pest Species of the S. (G.) chumpornense Subgroup (Simuliidae).

Two species of black flies (Simuliidae) in Thailand, Simulium chumpornense Takaoka and Kuvangkadilok, 2000, and S. khelangense Takaoka, Srisuka & Saeung, 2022, are potent vectors of avian blood protozoa of the genera Leucocytozoon and Trypanosoma and are pests of domestic avian species. Although the adults are abundant throughout Thailand, information on their breeding habitats is limited, and the immature stages of S. khelangense are unknown. We collected the larvae and pupae of S. khelangense from the Mekong River, the first-ever record of Simuliidae from this large continental river. Mitochondrial cytochrome c oxidase I and internal transcribed spacer 2 were used to associate the larvae and pupae with known adults. Both genetic markers strongly supported their identity as S. khelangense. The larvae and pupa of S. khelangense are described. The pupal gill filaments, larval abdominal protuberances, and setae distinguish this species from other members of the S. varicorne species group. The immature stages of S. chumpornense inhabit a wide variety of flowing waters, from small streams (3 m wide) to enormous continental rivers (400 m wide); thus, S. chumpornense is a habitat generalist. In contrast, S. khelangense was found only in the large Mekong River and is, therefore, a habitat specialist. Both species can exploit their principal habitats and produce abundant adult populations.