Long-distance Dispersal Research Articles

VO Supported on Functionalized CNTs for Oxidative Conversion of Furfural to Maleic Anhydride

Commercial non-functionalized (CNTs) and functionalized carbon nanotubes (CNT-COOH and CNT-NH2) were used as supports to synthesize vanadium-supported catalysts to be used in the gas phase partial oxidation of furfural towards maleic anhydride (MA). The CNTs and the VO2-V2O5/CNTs, so-called VO/CNT catalysts, were characterized by AAS, TGA, XRD, N2 adsorption isotherms at −196 °C, Raman, NH3-TPD and XPS. The surface area values, TGA and XRD results indicate that the larger thermal stability and larger dispersion of vanadium species is reached for the VO/CNT-NH2 catalyst. XPS indicates presence of surface VO2 and V2O5 species for the non-functionalized (CNT) and functionalized (CNT-COOH and CNT-NH2) catalysts, with a large interaction of the functional group with the surface vanadium species only for the VO/CNT-NH2 catalyst. The catalytic activity, evaluated in the range 305 °C to 350 °C, indicates that CO, CO2 and MA yield (%) and MA productivity are associated to the redox properties of the vanadium species, the oxygen exchange ability of the support and the vanadium–support interaction. For the reaction temperatures between 320 °C and 335 °C, the maximum MA yield (%) is found in the functionalized VO/CNT-COOH and VO/CNT-NH2 catalysts. This behavior is attributed to a decreased oxidation capability of the CNT with the functionalization. In addition, VO/CNT-NH2 is the more active and selective catalyst for MA productivity at 305 °C and 320 °C, which is related to the greater interaction of the surface vanadium species with the -NH2 group, which enhances the redox properties and stabilization of the VO2 and V2O5 surface active sites. Recycling at 350 °C resulted in 100% furfural conversion for all catalysts and a similar MA yield (%) compared to the fresh catalyst, indicating no loss of surface active sites.

Wind-mediated Dispersal of Beet Leafhoppers and Pine Pollen in Southern Idaho

The management of beet leafhoppers (BLHs) is important for disease control since BLHs can vector important plant pathogens such as curly top viruses and phytoplasmas in southern Idaho. Historical data for southern Idaho suggests that BLH need approximately 130 growing degree days (GDDs; 12.8°C base) to initiate dispersal and around 382 GDDs until they reach peak dispersal. A recent study in southern Idaho identified large peak dispersal events of BLHs on May 19, 2020 and June 2, 2021 in Elmore Co. near Mt. Home, ID. Historically, BLH have been thought to originate from local areas. However, based on GDDs and dispersal numbers under optimal conditions for Mt. Home, the BLHs likely did not originate from local areas. Data for wind and pine pollen dispersal combined with GDDs for areas known to contain BLH suggest that the BLH could have originated outside the local area and possibly up to 142 to 515 km away. At least five conditions appear to be necessary for observation of BLH dispersal into southern Idaho: a wind event must occur (35 km/h avg. hourly wind speed), dispersal temperature threshold (16 to 18°C) must be met, >130 GDDs must be accumulated to initiate dispersal, daily peak temperatures should reach 24°C, and attractive BLH vegetation such as Russian thistle must be present. Combining wind event forecasts with temperature parameters in the future may make it possible to provide targeted timely insecticide sprays for BLH control.

Resonant and Ultra-short-period Planet Systems Are at Opposite Ends of the Exoplanet Age Distribution

Exoplanet systems are thought to evolve on secular timescales over billions of years. This evolution is impossible to directly observe on human timescales in most individual systems. While the availability of accurate and precise age inferences for individual exoplanet host stars with ages τ in the interval 1 Gyr ≲ τ ≲ 10 Gyr would constrain this evolution, accurate and precise age inferences are difficult to obtain for isolated field dwarfs like the host stars of most exoplanets. The Galactic velocity dispersion of a thin-disk stellar population monotonically grows with time, and the relationship between age and velocity dispersion in a given Galactic location can be calibrated by a stellar population for which accurate and precise age inferences are possible. Using a sample of subgiants with precise age inferences, we calibrate the age–velocity dispersion relation in the Kepler field. Applying this relation to the Kepler field’s planet populations, we find that Kepler-discovered systems plausibly in second-order mean-motion resonances have 1 Gyr ≲ τ ≲ 2 Gyr. The same is true for systems plausibly in first-order mean-motion resonances, but only for systems likely affected by tidal dissipation inside their innermost planets. These observations suggest that many planetary systems diffuse away from initially resonant configurations on secular timescales. Our calibrated relation also indicates that ultra-short-period (USP) planet systems have typical ages in the interval 5 Gyr ≲ τ ≲ 6 Gyr. We propose that USP planets tidally migrated from initial periods in the range 1 day ≲ P ≲ 2 days to their observed locations at P < 1 day over billions of years and trillions of cycles of secular eccentricity excitation and inside-planet damping.

Direct Microenvironment Modulation of CO2 Electroreduction: Negatively Charged Ag Sites Going beyond Catalytic Surface Reactions.

Electrochemical reduction of CO2 is an important way to achieve carbon neutrality, and much effort has been devoted to the design of active sites. Apart from elevating the intrinsic activity, expanding the functionality of active sites may also boost catalytic performance. Here we designed "negatively charged Ag (nc-Ag)" active sites featuring both the intrinsic activity and the capability of regulating microenvironment, through modifying Ag nanoparticles with atomically dispersed Sn species. Different from conventional active sites (which only mediate the surface processes by bonding with the intermediates), the nc-Ag sites could also manipulate environmental species. Therefore, the sites could not only activate CO2, but also regulate interfacial H2O and CO2, as confirmed by operando spectroscopies. The catalyst delivers a high current density with a CO faradaic efficiency of 97 %. Our work here opens up new opportunities for the design of multifunctional electrocatalytic active sites.

Direct Microenvironment Modulation of CO2 Electroreduction: Negatively Charged Ag Sites Going beyond Catalytic Surface Reactions

AbstractElectrochemical reduction of CO2 is an important way to achieve carbon neutrality, and much effort has been devoted to the design of active sites. Apart from elevating the intrinsic activity, expanding the functionality of active sites may also boost catalytic performance. Here we designed “negatively charged Ag (nc‐Ag)” active sites featuring both the intrinsic activity and the capability of regulating microenvironment, through modifying Ag nanoparticles with atomically dispersed Sn species. Different from conventional active sites (which only mediate the surface processes by bonding with the intermediates), the nc‐Ag sites could also manipulate environmental species. Therefore, the sites could not only activate CO2, but also regulate interfacial H2O and CO2, as confirmed by operando spectroscopies. The catalyst delivers a high current density with a CO faradaic efficiency of 97 %. Our work here opens up new opportunities for the design of multifunctional electrocatalytic active sites.

Pointwise estimates on the fundamental solution to nonlocal diffusion equation

In this article we study the pointwise behavior of the fundamental solution S ( t , x ) to the nonlocal diffusion model u t = D ( J ∗ u − u ) on R , S ( t , x ) can be written as e − Dt δ ( x ) + S 0 ( t , x ) , where S 0 ( t , x ) is called the regularizing part. Under some conditions on J and its Fourier transform J ^ , we obtain some new pointwise estimates on S 0 ( t , x ) . Then we apply our results to study the spatial dynamics of the nonlocal diffusion population model in a shifting environment considered in Li et al. [Spatial dynamics of a nonlocal dispersal population model in a shifting environment. J Nonlinear Sci. 2018;28:1189–1219]. In our study the convolution kernel J can be assumed to be more general, i.e. not necessarily of compact support, also the proof is greatly simplified. It is our believe that these pointwise estimates possess their own interest in further studying nonlocal diffusion models.

Association in Like-Charged Surfactant-Nanoparticle Systems: Interfacial and Bulk Effects.

The association of similarly charged surfactant molecules and nanoparticles in an aqueous solution remains unresolved, and the understandings reported in the literature are conflicting. To address this issue, we undertake a fundamental study to investigate bulk and interfacial phenomena in binary mixtures of (i) positively charged nanoparticles and cationic surfactants and (ii) negatively charged nanoparticles and anionic surfactants. We find that the surfactant molecules adsorb on the surface of the nanoparticle despite similar charge, leading to supercharging of particles and simultaneously driving more surfactant molecules to the air-dispersion interface. Hence, the properties of the dispersed species, such as the size and zeta potential, and the interfacial properties, such as the surface tension and surface excess concentration, change significantly. This effect is more pronounced at a low surfactant concentration and is observed irrespective of the size of nanoparticles and surfactant-particle combination. Further, we elucidate the important role of electrostatic interactions in the surfactant-particle complexation process by varying the pH of the dispersions. Contrary to changes in the properties of the dispersed species and interface, the presence of particles does not appreciably change the bulk property, such as the critical micelle concentration.

Intraspecific variation in group structure arises due to environmentally-mediated directional dispersal in a cooperative breeder.

Many cooperatively breeding species live in groups with complex structure-large group sizes, low and variable kin structure, and multiple breeding pairs. Since these mixed-kin groups typically form because of immigration of unrelated individuals of both sexes in addition to limited offspring dispersal, differences in patterns of dispersal can generate variation in group structure, even within the same species or population. Here, we examine how environmentally mediated dispersal patterns influence variation in group structure in the plural breeding superb starling (Lamprotornis superbus), an avian cooperative breeder that inhabits a spatiotemporally variable savanna environment and forms mixed-kin groups with variable group sizes and more than one breeding pair per group. Using 4068 genome-wide polymorphic loci and fine-scale, remotely sensed ecological data from 22 groups sampled across a nearly 200 km2 environmental gradient in central Kenya, we find evidence of not only frequent and long-distance dispersal in both sexes (low isolation-by-distance and weak genetic structure), but also directional dispersal from small groups in lower quality habitat with low normalised difference vegetation index (NDVI) to large groups in higher quality habitat with high NDVI. Additionally, we find stronger genetic structure among groups in lower quality habitat, and higher genetic diversity and lower relatedness of groups in higher quality habitat. Previous work using long-term data from groups in the same population has shown that groups with lower relatedness are larger and have more breeding pairs. Long-distance, directional dispersal to maximise individual fitness can thus lead to smaller and simpler kin-based social groups in lower quality habitat, but larger and more complex mixed-kin groups in higher quality habitat. Such intraspecific, within-population variation in group structure, including variation in kin structure of social groups, could have profound implications for the relative importance of the evolutionary mechanisms (i.e. direct vs. indirect fitness benefits) underlying the formation of cooperative societies.

Adaptation of Fusarium Head Blight Pathogens to Changes in Agricultural Practices and Human Migration.

Fusarium head blight (FHB) is one of the most destructive wheat diseases worldwide. To understand the impact of human migration and changes in agricultural practices on crop pathogens, here population genomic analysis with 245 representative strains from a collection of 4,427 field isolates of Fusarium asiaticum, the causal agent of FHB in Southern China is conducted. Three populations with distinct evolution trajectories are identifies over the last 10,000 years that can be correlated with historically documented changes in agricultural practices due to human migration caused by the Southern Expeditions during the Jin Dynasty. The gradual decrease of 3ADON-producing isolates from north to south along with the population structure and spore dispersal patterns shows the long-distance (>250km) dispersal of F. asiaticum. These insights into population dynamics and evolutionary history of FHB pathogens are corroborated by a genome-wide analysis with strains originating from Japan, South America, and the USA, confirming the adaptation of FHB pathogens to cropping systems and human migration.

MOFs-derived CuCeOx supported on H-zeolite socony mobile-5 for catalytic oxidation of 1,2-dichlorobenzene

The catalytic elimination of chlorinated aromatic hydrocarbons is the frontier of catalytic oxidation of organic pollutants, and the screen of efficient catalysts (low cost, high activity, durability and selectivity) remains challenging. In this work, the CuCeOx derived from metal-organic frameworks supported on H-zeolite socony mobile-5 (HZSM-5) complex catalyst (CuCeOx-H-C) was prepared through impregnation combined with pyrolysis and applied in the catalytic oxidation of 1,2-dichlorobenzene. The results indicate that the introduction of carboxyl groups on the surface of HZSM-5 is crucial for the formation of Ce-MOF/HZSM-COOH core–shell structure. This introduction facilitates the uniform deposition of CuCeOx on HZSM-5 and significantly enhances the overall acidity. Furthermore, the synergistic effect of Cu-Ce significantly enhanced the catalyst’s activity at low temperatures. Therefore, CuCeOx-H-C exhibited the highest activity, with a T90 of 236 °C. Additionally, the CuCeOx-H-C catalyst showed high selectivity for HCl and suppressed the generation of chlorinated by-products. The differences in catalyst activity among various catalysts arise from multiple factors, including specific surface area, dispersion of active species, oxygen mobility, oxygen storage capacity, redox properties, and others. Under the synergetic effects of above-mentioned factors, the activity of CuCeOx-H-C is remarkably enhanced and would be promising for potential industrial application.

Inbreeding avoidance, competition and natal dispersal in a pair-living, genetically monogamous mammal, Azara's owl monkey (Aotus azarae).

Natal dispersal is an important life-history stage influencing individual fitness, social dynamics of groups and population structure. Understanding factors influencing dispersal is essential for evaluating explanations for the evolution and maintenance of social organization, including parental care and mating systems. The social and mating systems of Azara's owl monkeys (Aotus azarae) are infrequent among mammals; these primates are pair-living, serially and genetically monogamous and both sexes directly care for offspring. To evaluate the role that competition and inbreeding avoidance play in shaping dispersal patterns, we used 25 years of demographic and genetic data to examine how variation in timing of natal dispersal is related to social (adult replacements, step-parents, births and group size) and ecological factors (seasonal abundance of resources) in a wild population of A. azarae in Formosa, Argentina. We found that all males and females dispersed from their natal groups, but subadults delayed dispersal when a step-parent of the opposite sex joined the group, indicating that they may perceive these step-parents as potential mates. Dispersal was more probable when resource conditions were better, regardless of age. Overall, agonistic conflict over food and potential mates with adults in the natal group, as well as inbreeding avoidance, contribute to regulating dispersal.

Dispersal of a wolf with complex ancestry from the Dinaric–Alpine region to Germany demonstrates the value of transboundary monitoring networks

AbstractLarge carnivores such as wolves (Canis lupus) at times disperse distances of several hundred kilometers, which may enable gene flow over vast distances. Such long‐distance dispersal events, however, often remain undetected, and documenting long‐distance dispersers and their movements typically depend on strong transboundary collaborations. Here, we report the dispersal of a wolf (MSV0FF) from the Alpine region of northern Slovenia to Bavaria in southern Germany, a straight‐line distance of around 300 km. The disperser originated in a pack with a genetically diverse breeding pair, where the father exhibited ancestry from the Dinaric and Italian Alpine populations. Genetic analysis of the mother indicated that she was an immigrant from a divergent population further south in the Dinaric‐Balkan region. Such a varied ancestry augments the probability of immigrants being genetically misclassified as wolf‐dog hybrids in their area of arrival, which increases the risk of unfavorable management decisions toward individuals that would be genetically highly valuable for the recipient population. Wolf MSV0FF therefore demonstrates the benefits of international collaborative monitoring networks and the value of sharing samples and analytical approaches for the monitoring of wide‐ranging species.

Gone with the waves: the role of sea currents as key dispersal mechanism for Mediterranean coastal and inland plant species.

Thalassochory, the dispersal of propagules through marine currents, is a key long-distance dispersal (LDD) mechanism with implications for global biogeography and particularly for island colonization. The propagules of coastal plant species are generally assumed to be better adapted for sea dispersal than those of inland plants, but this hypothesis remains largely untested. We conducted experiments on four genera (Juniperus, Daucus, Ferula, and Pancratium) and compared traits among nine species with different habitats and distributions. Our results showed that Juniperus spp. and P. maritimum have strong thalassochorous potential within the Mediterranean Basin. Interestingly, we did not find a clear association on the thalassochorous potential of coastal versus inland species within all the tested genera, apart from P. maritimum compared withthe endemic inland P.illyricum. These findings suggest that thalassochory may be a more common dispersal mechanism than previously assumed. The apparently weak link of dispersal syndrome with species ecology broadens the possibility of dispersal by the sea also for inland plants, although considered to be poorly salt-tolerant. Moreover, our results reveal significant differences in sea dispersal between endemic and widespread species, but do not rule out an important role of thalassochory in shaping the distribution patterns of archipelago endemic flora. The presented method is largely replicable and could be used for further studies with a larger set of species to better delineate trends of sea dispersal syndrome among species with different ecology or dispersal traits.

Landscape, demographic, and dispersal parameters influence the spread and establishment of an aquatic biological invasion

AbstractHuman‐mediated species introductions are contributing to the biotic homogenization of global flora and fauna. Despite extensive research, we lack simple methods of predicting how and where an introduced species will spread and establish, particularly in species with complex life histories in aquatic ecosystems. We predict that spread can be modeled simply using the characteristics of the invading population, specifically species growth rate and dispersal capacity. In addition, we predict that the establishment of introduced species should be explained by the characteristics of the receiving ecosystem. Using the brown trout (Salmo trutta) invasion on the Island of Newfoundland as a case study, we fit and test a reaction–diffusion model with brown trout population data collected from the literature. Next, we use statistical models to assess the influence of a suite of abiotic (conductivity, pH, turbidity, calcium), biotic (Atlantic salmon occurrence), and landscape (watershed relief, watershed area, distance to original introduction) variables on brown trout establishment (i.e., presence–absence) patterns. We find that observed coastal spread in Newfoundland is slow (~4.4 km/year), and that it lies on the lower end of the range of predictions made by the reaction–diffusion model parameterized based on the estimates of growth rate and movement from the literature (predicted spread range 1.4 to 92 km/year). Also, we did not find evidence for a relationship between abiotic or biotic variables and brown trout establishment. However, we did observe that landscape variables of the distance to trout introduction point and estuary area may explain establishment patterns along the south coast of Newfoundland. Our results suggest the importance of using population‐specific parameterization and the need to integrate regional landscape factors that are generally applicable across biological invasions (e.g., distance to introduction), and those that are more specific to the ecology of the invader (e.g., estuary area). Our study contextualizes the mechanisms that contribute to a slow invasion by an aquatic species with a complex life history and reveals that future studies need to integrate a variety of methods to elucidate the processes governing invasions.

Reliable biogeography requires fossils: insights from a new species-level phylogeny of extinct and living carnivores.

A central objective of historical biogeography is to understand where clades originated and how they moved across space and over time. However, given the dynamic history of ecosystem changes in response to climate change and geological events, the manifold long-distance dispersals over evolutionary timescales, and regional and global extinctions, it remains uncertain how reliable inferences based solely on extant taxa can be achieved. Using a novel species-level phylogeny of all known extant and extinct species of the mammalian order Carnivora and related extinct groups, we show that far more precise and accurate ancestral areas can be estimated by fully integrating extinct species into the analyses, rather than solely relying on extant species or identifying ancestral areas only based on the geography of the oldest fossils. Through a series of simulations, we further show that this conclusion is robust under realistic scenarios in which the unknown extinct taxa represent a biased subset of all extinct species. Our results highlight the importance of integrating fossil taxa into a phylogenetic framework to further improve our understanding of historical biogeography and reveal the dynamic dispersal and diversification history of carnivores.

Densovirus infection facilitates plant-virus transmission by an aphid.

The interactions among plant viruses, insect vectors, and host plants have been well studied; however, the roles of insect viruses in this system have largely been neglected. We investigated the effects of MpnDV infection on aphid and PVY transmission using bioassays, RNA interference (RNAi), and GC-MS methods and green peach aphid (Myzus persicae (Sulzer)), potato virus Y (PVY), and densovirus (Myzus persicae nicotianae densovirus, MpnDV) as model systems. MpnDV increased the activities of its host, promoting population dispersal and leading to significant proliferation in tobacco plants by significantly enhancing the titer of the sesquiterpene (E)-β-farnesene (EβF) via up-regulation of expression levels of the MpFPPS1 gene. The proliferation and dispersal of MpnDV-positive individuals were faster than that of MpnDV-negative individuals in PVY-infected tobacco plants, which promoted the transmission of PVY. These results combined showed that an insect virus may facilitate the transmission of a plant virus by enhancing the locomotor activity and population proliferation of insect vectors. These findings provide novel opportunities for controlling insect vectors and plant viruses, which can be used in the development of novel management strategies.

Enhancing Water Tolerance and N2 Selectivity in NH3-SCR Catalysts by Protecting Mn Oxide Nanoparticles in a Silicalite-1 Layer.

Mn-based catalysts are promising candidates for eliminating harmful nitrogen oxides (NOx) via selective catalytic reduction with ammonia (NH3-SCR) due to their inherent strong redox abilities. However, poor water tolerance and low N2 selectivity are still the main limitations for practical applications. Herein, we succeeded in preparing an active catalyst for NH3-SCR with improved water tolerance and N2 selectivity based on protecting MnOx with a secondary growth of a hydrophobic silicalite-1. This protection suppressed catalyst deactivation by water adsorption. Interestingly, impregnating MnOx on MesoTS-1 followed by silicalite-1 protection allowed for a higher dispersion of MnOx species, thus increasing the concentration of acid sites. Consequently, the level of N2O formation is decreased. These improvements resulted in a broader operating temperature of NOx conversion and a modification of the NH3-SCR mechanism. Diffuse reflectance infrared Fourier transform spectroscopy analysis revealed that unprotected Mn/MesoTS-1 mainly followed the Eley-Rideal mechanism, while Mn/MesoTS-1@S1 followed both Langmuir-Hinshelwood and Eley-Rideal mechanisms.

Seed dispersal effectiveness by greater one-horned rhinos and domestic bovids of a megafaunal fruit

The extinction of megafauna, such as rhinos, from tropical Asian forests may have detrimental effects on the regeneration of plant species that rely heavily on their dispersal services. Understanding the potential of other animals to substitute the ecological function of megafauna is important for predicting ecosystem change following declines or extirpation of megafauna. Anthropogenic activities have led to the presence of domestic bovids in many tropical habitats, and these animals could be capable of substituting seed dispersal roles of megafauna. Here, we conducted feeding trials, germination tests, and collected movement data on greater one-horned rhino (Rhinoceros unicornis) and domestic bovids (Bos primigenius; Bubalus bubalis) in the national parks of Chitwan and Shuklaphanta, Nepal. Our aim was to investigate whether domestic bovids can serve as effective substitutes for rhinos in dispersing the megafaunal-fruited Mallotus nudiflorus, which are specialized for rhino dispersal. Specifically, we assessed the long-distance seed dispersal capabilities of rhinos and bovids by combining gut passage times and movement patterns. Additionally, we compared the seed germination success rates among rhinos, bovids and other dispersal modes (e.g., deer and water dispersal). We found that rhinos dispersed 80 percent of seeds over distances greater than 1 km, with potential dispersal distances of up to approximately 5.4 km within their maximum gut passage time. In contrast, cattle dispersed 20 percent of seeds over 1 km, with a maximum distance of around 2.2 km, while buffaloes dispersed 15 percent of seeds over 1 km, with a maximum distance of around 1.4 km. Seeds consumed by rhinos exhibited similar or better germination rates compared to seeds retrieved from cattle and buffalo dung, as well as other dispersal modes. These findings indicate that rhinos provide a functionally unique dispersal service for this megafaunal fruit, especially in long-distance dispersal. While domestic bovids can partially substitute the seed dispersal functions of rhinos, they cannot fully compensate for the loss of seed dispersal that occurs with the disappearance of rhinos.

Stable and efficient chlorine evolution reaction with atomically dispersed Ru on surface tensile strained TiO2

Developing highly efficient and selective electrocatalysts for the chlorine evolution reaction (CER) in the chloralkali industry is of great importance. Here, we report the discovery of a new electrocatalyst for CER consisting of atomically dispersed Ru sites on surface tensile strained TiO2 (Ru-S-TiO2). The single-atom Ru species were stabilized on the strained TiO2 surface by strong metal-support interactions. The Ru-S-TiO2 is highly efficient, initiating CER at only 5 mV above the ECER, and has shown excellent stability for over 100 hours. It exhibited >95 % CER selectivity even in acidic media with low Cl− concentrations (0.2 M). Our results demonstrate that the strong metal-support interactions between the atomically dispersed Ru species and the strained TiO2 surface are crucial for the high catalytic activity, selectivity, and stability of Ru-S-TiO2 for CER. Ru-S-TiO2 holds great promise as a viable alternative to existing mixed metal oxides-based electrocatalysts for CER in the chloralkali industry.

Distinct Communities and Differing Dispersal Routes in Bacteria and Fungi of Honey Bees, Honey, and Flowers

Microbiota, the communities of microbes on and in organisms or organic matter, are essential for the functioning of ecosystems. How microbes are shared and transmitted delineates the formation of a microbiota. As pollinators forage, they offer a route to transfer microbes among the flowering plants, themselves, and their nests. To assess how the two components of the microbiota, bacteria and fungi, in pollination communities are shared and transferred, we focused on the honey bee Apis mellifera and collected honey bee, honey (representing the hive microbiota), and flower samples three times during the summer in Finland. We identified the bacteria and fungi by DNA metabarcoding. To determine the impact of honey bees’ flower choices on the honey bee and hive microbiota, we identified also plant DNA in honey. The bacterial communities of honey bees, honey, and flowers all differ greatly from each other, while the fungal communities of honey bees and honey are very similar, yet different from flowers. The time of the summer and the sampling area influence all these microbiota. For flowers, the plant identity impacts both bacterial and fungal communities’ composition the most. For the dispersal pathways of bacteria to honey bees, they are acquired directly from the honey and indirectly from flowers through the honey, while fungi are directly transmitted to honey bees from flowers. Overall, the distinctiveness of the microbiota of honey bees, honey, and the surrounding flowers suggests the sharing of microbes among them occurs but plays a minor role for the established microbiota.